Col11a2 (NM_212528) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209681 representing NM_212528 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGTGCAGCCGCTGCCACCGCCTCCTACTGCTCCTACCTCTGGTGCTGGGTCTGAGTGCTGCCC CAGGATGGGCAGGTGCACCCTCTGTGGATGTGCTACGTGCCCTGAGGTTCCCCTCCCTTCCCGATGGTGT TCGGAAATCAAAAGGGGTCTGTCCAGCTGATGTGGCTTACCGTGTGGCACGACCTGCCCAGCTCAGCGCA CCCACACGCCAGCTCTTCCCAGGAGGCTTTCCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACCCGCT CTGGCCTCCAAGCTCCCCTCCTGACTCTATACAGTGCCCAGGGAGTCCAGCAACTGGGCCTGGAGCTCGG TCGTCCAGTCCGCTTTCTCTATGAGGACCAGAGGGGACGGCCACAAGCCTCCGCTCAGCCCATCTTCCGA GGCCTGAGCCTCGCTGATGGCAAGTGGCACCATGTGGCTGTAGCTGTGAAGGGTCAGTCTGTCACTCTCA TTGTGGACTGTAAGAAGCGAGTCACCCGGCCCCTTCCCCGAAGCGTGCATCCGGTGCTGGACACCCACGG GGTGGTGATCTTTGGTGCCCACGTCCTCGATGATGAAGTCTTTGAAGGTGATGTTCAGGAACTCCTCATT GTCCCGGGCGTCCAAGCTGCCTATCAGTCTTGTGGACAGAAGGACCTGGAATGCGAGAGAGAACAGAGGG ACGGGCCTCAGACTCAGAAGCCTCACAGGGCCCAGAGATCTCCAAAGAAGGAGCCAGCCAGACTTCATAA GCCACAGAACCAGGAGCCCCAGCAGCAGTACCCCACCCCAGGTGAAGAGGAAGGAGTCCTGGAGTCCAGC CTCTTGCCATCTCTTGAAGAGGTTGCCCACGGACCCCGGGGGCTAAAGGGAGAGAAGGGAGAGCCTGCAG TGCTGGAGCCTGGTATGTTTGTAGAGGGACCTCCAGGCCCAGAAGGCCCAGCGGGGTTAGCTGGACCCCC TGGCATCCAGGGGAACCCAGGCCCAGTTGGAGACCCTGGCGAGAGGGGCCCCCCTGGCCGGGCAGGGCTC CCTGGATCAGACGGACCTCCTGGTCCTCCTGGTACATCTCTGATGCTCCCATTCCGGTTTGGCAGCAGTG GTGGTGACAAGGGCCCCGTGGTGGCAGCCCAGGAGGCCCAGGCCCAGGCAATTCTGCAGCAGGCACGGCT GGCCCTCCGTGGGCCCCCTGGCCCCATGGGATACACGGGCCGTCCTGGACCGTTGGGTCAGCCTGGGAGC CCTGGCTTGAAGGGAGAATCTGGAGACCTGGGCCCACAGGGCCCCAGAGGACCTCAGGGCCTCACAGGTC CTCCTGGCAAGGCTGGGCGAAGGGGCCGAGCAGGTGCTGATGGAGCCCGTGGGATGCCCGGAGAACCTGG CGTGAAGGGTGACCGAGGCTTTGACGGACTGCCTGGGCTACCTGGTGAAAAGGGACAAAGGGGTGACACA GGTGCTCAGGGCCTTCCTGGGCCTCCCGGTGAGGACGGAGAGAGGGGTGACGATGGAGAGATTGGGCCCC GCGGGCTGCCTGGAGAGTCAGGACCTAGAGGACTCCTTGGCCCTAAAGGCCCTCCTGGTATTCCTGGGCC CCCGGGACCTCAAGGAGAGCCAGGGCCTCCCGGACAACAGGGCACTCCTGGAACTCAGGGCCTCCCCGGA CCTCAGGGTGCCATTGGACCTCATGGAGAGAAGGGCCCTCGTGGGAAACCAGGCCTCCCTGGCATGCCGG GATCAGATGGACTCCCGGGCCACCCAGGGAAGGAAGGACCCCCTGGAACCAAAGGGAACCAGGGCCCCTC CGGACCTCAGGGTCCTTTAGGATACCCAGGCCCTCGAGGCGTCAAGGGCGTGGATGGGATTCGGGGCCTG AAGGGCCACAAGGGTGAAAAGGGTGAGGACGGCTTTCCTGGATTCAAAGGTGACATAGGTGTGAAAGGAG ACAGGGGCGAGGTCGGAGTCCCTGGTTCCAGGGGAGAAGATGGCCCCGAAGGGCCGAAAGGGCGCACTGG ACCTACCGGAGACCCCGGGCCCACGGGGCTCATGGGCGAGAAGGGCAAGCTAGGTGTTCCTGGTCTGCCT GGCTACCCCGGACGCCAGGGTCCCAAGGGATCTCTGGGTTTTCCTGGTTTTCCTGGAGCCAGTGGAGAGA AGGGAGCTCGGGGCCTGTCTGGGAAATCAGGACCTCGGGGAGAACGGGGCCCCACGGGTCCAAGGGGTCA GCGGGGACCTCGAGGTGCCACTGGGAAGTCTGGAGCTAAGGGAACATCAGGTGGTGATGGTCCTCACGGG CCGCCCGGAGAGAGGGGTCTTCCTGGACCGCAAGGCCCCAATGGATTCCCTGGCCCCAAAGGCCCTCCGG GCCCCGCAGGGAAAGACGGGCTGCCGGGACACCCAGGCCAGAGAGGAGAAGTGGGATTCCAAGGGAAGAC CGGCCCCCCAGGCCCGCCCGGAGTGGTGGGACCTCAGGGAGCAGCTGGGGAAAGCGGCCCCATGGGGGAG AGAGGTCACCCTGGCCCCCCAGGACCTCCTGGAGAGCAAGGGCTGCCCGGAACTGCTGGAAAAGATGGGA CCAAGGGTGACCCCGGGCCCCCCGGGGCCCCAGGGAAGGATGGCCCCGCTGGTCTGAGAGGCTTCCCAGG AGAGCGAGGCCTCCCGGGCACTGCTGGCGGACCTGGCTTGAAGGGAAATGAAGGCCCAGCTGGCCCTCCT GGCCCTGCAGGCTCTCCTGGCGAGCGAGGTGCAGCAGGATCAGGGGGCCCCATTGGTCCCCCTGGACGTC CAGGCCCACAGGGTCCCCCTGGGGCAGCAGGAGAGAAAGGCGTGCCGGGTGAGAAAGGCCCTATTGGTCC CACGGGTCGTGACGGGGTGCAGGGCCCTGTGGGGCTTCCTGGTCCCGCGGGACCCCCAGGCGTGGCAGGA GAGGATGGAGACAAGGGTGAGGTGGGCGACCCTGGGCAGAAGGGAACCAAAGGAAACAAGGGTGAGCATG GCCCTCCTGGCCCTCCTGGTCCCATCGGTCCTGTGGGGCAGCCTGGAGCTGCGGGAGCTGACGGCGAGCC CGGAGCCCGCGGACCCCAGGGACACTTTGGAGCCAAAGGTGACGAAGGAACAAGAGGGTTCAATGGGCCC CCTGGACCCATCGGCCTACAGGGCCTGCCAGGACCTTTTGGGGAAAAGGGAGAAACAGGAGACGTGGGGC CTATGGGACCCCCTGGCCCCCCAGGACCTCGAGGCCCCGCTGGACCCAATGGTGCTGATGGCCCACAAGG TCCCCCTGGAGGTGTCGGAAACTTGGGTCCCCCTGGAGAGAAGGGTGAACCAGGGGAGTCGGGGTCTCCA GGAGTCCAGGGTGAGCCGGGCGTCAAGGGTCCACGTGGAGAGCGTGGTGAGAAAGGAGAGTCTGGGCAGG CAGGAGAGGCTGGACCACCAGGGCCCAAAGGCCCTACAGGCGATGACGGCCCCAAGGGGAACCCTGGTCC CGTTGGCTTTCCTGGGGACCCTGGCCCCCCCGGAGAAGCTGGCCCAAGGGGCCAGGATGGTGCTAAGGGA AGCCAAGGCGAGGATGGCGAGCCGGGACAGCCTGGATCCCCCGGCCCCACCGGGGAGAACGGGCCCCCTG GACCTCTTGGAAAGCGGGGGGACCCTGGTGCTGTGGGGGCCCCAGGAAAGACGGGCCCTGTGGGCCCTGC AGGCCCAGCAGGGAAGCCTGGCCCCGATGGTCTTCGAGGGCTCCCTGGCTCAGTGGGTCAGCAAGGCCGC CCTGGAGCCACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGGACCCCCAGGGCTTCCTGGCCTCCGGGGTG ATGCTGGCGCCAAGGGGGAGAAGGGTCATCCAGGCCTCATCGGACTGATTGGGCCGACCGGAGAGCAAGG CGAGAAGGGTGACCGTGGCCTTCCTGGCCCTCAGGGCTCACCTGGACAAAAGGGAGAGACGGGTATCCCA GGAGCATCTGGTCCTATCGGTCCTGGAGGACCTCCTGGCCTGCCTGGACCGTCCGGCCCCAAAGGAGCTA AAGGAGCCACAGGCCCCGCTGGACCCAAAGGAGAGAAAGGTGTTCAGGGCCCTCCAGGACACCCGGGTCC CCCGGGCGAGGTGATCCAGCCACTGCCCATCCAGATGCCCAAGAAGACCCGGCGCTCTGTGGACGGAAGC AAACTGATGCAGGATGAGGAGGCCACACCCACTGGCGGTGCCCCGGGCAGTCCTGCAGGACTGGAGGAGA TCTTCGGTTCACTGGACTCTCTGCGGGAGGAGATCGAACAGATGAGGAGGCCGACAGGGACCCAGGACAG CCCTGCCCGCACCTGCCAGGACTTGAAGCTGTGCCACCCAGAGCTTCCCGATGGAGAGTACTGGGTTGAC CCCAACCAGGGCTGTGCCCGGGACGCCTTCCGGGTGTTCTGCAACTTTACAGCAGGAGGGGAGACTTGTG TCACACCCAGGGATGACGTCACACAGTTCTCCTACGTGGACTCTGAAGGCTCCCCAGTGGGCGTGGTCCA GCTCACCTTCCTGCGGCTGCTCAGCGTCTCTGCCCACCAGGATGTTTCCTACCCTTGCTCTGGGGTATCC CAGGACGGTCCCCTGAAACTCCGAGGGGCCAATGAGGATGAGCTGAGCCCTGAGACCAGCCCTTACGTCA AGGAGTTCAGAGATGGCTGTCAGACCCAGCAAGGCCGGACGGTGTTAGAGGTGCGCACGCCTGTGCTGGA GCAGCTGCCCGTGCTGGATGCTTCCTTCTCAGACCTGGGGGCCCCTACAAGACGGGGAGGGGTGCTTCTG GGGCCTGTCTGCTTCATGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209681 representing NM_212528 Red=Cloning site Green=Tags(s) MERCSRCHRLLLLLPLVLGLSAAPGWAGAPSVDVLRALRFPSLPDGVRKSKGVCPADVAYRVARPAQLSA PTRQLFPGGFPKDFSLLTVVRTRSGLQAPLLTLYSAQGVQQLGLELGRPVRFLYEDQRGRPQASAQPIFR GLSLADGKWHHVAVAVKGQSVTLIVDCKKRVTRPLPRSVHPVLDTHGVVIFGAHVLDDEVFEGDVQELLI VPGVQAAYQSCGQKDLECEREQRDGPQTQKPHRAQRSPKKEPARLHKPQNQEPQQQYPTPGEEEGVLESS LLPSLEEVAHGPRGLKGEKGEPAVLEPGMFVEGPPGPEGPAGLAGPPGIQGNPGPVGDPGERGPPGRAGL PGSDGPPGPPGTSLMLPFRFGSSGGDKGPVVAAQEAQAQAILQQARLALRGPPGPMGYTGRPGPLGQPGS PGLKGESGDLGPQGPRGPQGLTGPPGKAGRRGRAGADGARGMPGEPGVKGDRGFDGLPGLPGEKGQRGDT GAQGLPGPPGEDGERGDDGEIGPRGLPGESGPRGLLGPKGPPGIPGPPGPQGEPGPPGQQGTPGTQGLPG PQGAIGPHGEKGPRGKPGLPGMPGSDGLPGHPGKEGPPGTKGNQGPSGPQGPLGYPGPRGVKGVDGIRGL KGHKGEKGEDGFPGFKGDIGVKGDRGEVGVPGSRGEDGPEGPKGRTGPTGDPGPTGLMGEKGKLGVPGLP GYPGRQGPKGSLGFPGFPGASGEKGARGLSGKSGPRGERGPTGPRGQRGPRGATGKSGAKGTSGGDGPHG PPGERGLPGPQGPNGFPGPKGPPGPAGKDGLPGHPGQRGEVGFQGKTGPPGPPGVVGPQGAAGESGPMGE RGHPGPPGPPGEQGLPGTAGKDGTKGDPGPPGAPGKDGPAGLRGFPGERGLPGTAGGPGLKGNEGPAGPP GPAGSPGERGAAGSGGPIGPPGRPGPQGPPGAAGEKGVPGEKGPIGPTGRDGVQGPVGLPGPAGPPGVAG EDGDKGEVGDPGQKGTKGNKGEHGPPGPPGPIGPVGQPGAAGADGEPGARGPQGHFGAKGDEGTRGFNGP PGPIGLQGLPGPFGEKGETGDVGPMGPPGPPGPRGPAGPNGADGPQGPPGGVGNLGPPGEKGEPGESGSP GVQGEPGVKGPRGERGEKGESGQAGEAGPPGPKGPTGDDGPKGNPGPVGFPGDPGPPGEAGPRGQDGAKG SQGEDGEPGQPGSPGPTGENGPPGPLGKRGDPGAVGAPGKTGPVGPAGPAGKPGPDGLRGLPGSVGQQGR PGATGQAGPPGPVGPPGLPGLRGDAGAKGEKGHPGLIGLIGPTGEQGEKGDRGLPGPQGSPGQKGETGIP GASGPIGPGGPPGLPGPSGPKGAKGATGPAGPKGEKGVQGPPGHPGPPGEVIQPLPIQMPKKTRRSVDGS KLMQDEEATPTGGAPGSPAGLEEIFGSLDSLREEIEQMRRPTGTQDSPARTCQDLKLCHPELPDGEYWVD PNQGCARDAFRVFCNFTAGGETCVTPRDDVTQFSYVDSEGSPVGVVQLTFLRLLSVSAHQDVSYPCSGVS QDGPLKLRGANEDELSPETSPYVKEFRDGCQTQQGRTVLEVRTPVLEQLPVLDASFSDLGAPTRRGGVLL GPVCFMG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_212528
ORF Size	4851 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_212528.1, NP_997693.1
RefSeq Size	5951 bp
RefSeq ORF	4854 bp
Locus ID	294279
MW	159 kDa

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