Col11a2 (NM_212528) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209681 representing NM_212528 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGTGCAGCCGCTGCCACCGCCTCCTACTGCTCCTACCTCTGGTGCTGGGTCTGAGTGCTGCCC CAGGATGGGCAGGTGCACCCTCTGTGGATGTGCTACGTGCCCTGAGGTTCCCCTCCCTTCCCGATGGTGT TCGGAAATCAAAAGGGGTCTGTCCAGCTGATGTGGCTTACCGTGTGGCACGACCTGCCCAGCTCAGCGCA CCCACACGCCAGCTCTTCCCAGGAGGCTTTCCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACCCGCT CTGGCCTCCAAGCTCCCCTCCTGACTCTATACAGTGCCCAGGGAGTCCAGCAACTGGGCCTGGAGCTCGG TCGTCCAGTCCGCTTTCTCTATGAGGACCAGAGGGGACGGCCACAAGCCTCCGCTCAGCCCATCTTCCGA GGCCTGAGCCTCGCTGATGGCAAGTGGCACCATGTGGCTGTAGCTGTGAAGGGTCAGTCTGTCACTCTCA TTGTGGACTGTAAGAAGCGAGTCACCCGGCCCCTTCCCCGAAGCGTGCATCCGGTGCTGGACACCCACGG GGTGGTGATCTTTGGTGCCCACGTCCTCGATGATGAAGTCTTTGAAGGTGATGTTCAGGAACTCCTCATT GTCCCGGGCGTCCAAGCTGCCTATCAGTCTTGTGGACAGAAGGACCTGGAATGCGAGAGAGAACAGAGGG ACGGGCCTCAGACTCAGAAGCCTCACAGGGCCCAGAGATCTCCAAAGAAGGAGCCAGCCAGACTTCATAA GCCACAGAACCAGGAGCCCCAGCAGCAGTACCCCACCCCAGGTGAAGAGGAAGGAGTCCTGGAGTCCAGC CTCTTGCCATCTCTTGAAGAGGTTGCCCACGGACCCCGGGGGCTAAAGGGAGAGAAGGGAGAGCCTGCAG TGCTGGAGCCTGGTATGTTTGTAGAGGGACCTCCAGGCCCAGAAGGCCCAGCGGGGTTAGCTGGACCCCC TGGCATCCAGGGGAACCCAGGCCCAGTTGGAGACCCTGGCGAGAGGGGCCCCCCTGGCCGGGCAGGGCTC CCTGGATCAGACGGACCTCCTGGTCCTCCTGGTACATCTCTGATGCTCCCATTCCGGTTTGGCAGCAGTG GTGGTGACAAGGGCCCCGTGGTGGCAGCCCAGGAGGCCCAGGCCCAGGCAATTCTGCAGCAGGCACGGCT GGCCCTCCGTGGGCCCCCTGGCCCCATGGGATACACGGGCCGTCCTGGACCGTTGGGTCAGCCTGGGAGC CCTGGCTTGAAGGGAGAATCTGGAGACCTGGGCCCACAGGGCCCCAGAGGACCTCAGGGCCTCACAGGTC CTCCTGGCAAGGCTGGGCGAAGGGGCCGAGCAGGTGCTGATGGAGCCCGTGGGATGCCCGGAGAACCTGG CGTGAAGGGTGACCGAGGCTTTGACGGACTGCCTGGGCTACCTGGTGAAAAGGGACAAAGGGGTGACACA GGTGCTCAGGGCCTTCCTGGGCCTCCCGGTGAGGACGGAGAGAGGGGTGACGATGGAGAGATTGGGCCCC GCGGGCTGCCTGGAGAGTCAGGACCTAGAGGACTCCTTGGCCCTAAAGGCCCTCCTGGTATTCCTGGGCC CCCGGGACCTCAAGGAGAGCCAGGGCCTCCCGGACAACAGGGCACTCCTGGAACTCAGGGCCTCCCCGGA CCTCAGGGTGCCATTGGACCTCATGGAGAGAAGGGCCCTCGTGGGAAACCAGGCCTCCCTGGCATGCCGG GATCAGATGGACTCCCGGGCCACCCAGGGAAGGAAGGACCCCCTGGAACCAAAGGGAACCAGGGCCCCTC CGGACCTCAGGGTCCTTTAGGATACCCAGGCCCTCGAGGCGTCAAGGGCGTGGATGGGATTCGGGGCCTG AAGGGCCACAAGGGTGAAAAGGGTGAGGACGGCTTTCCTGGATTCAAAGGTGACATAGGTGTGAAAGGAG ACAGGGGCGAGGTCGGAGTCCCTGGTTCCAGGGGAGAAGATGGCCCCGAAGGGCCGAAAGGGCGCACTGG ACCTACCGGAGACCCCGGGCCCACGGGGCTCATGGGCGAGAAGGGCAAGCTAGGTGTTCCTGGTCTGCCT GGCTACCCCGGACGCCAGGGTCCCAAGGGATCTCTGGGTTTTCCTGGTTTTCCTGGAGCCAGTGGAGAGA AGGGAGCTCGGGGCCTGTCTGGGAAATCAGGACCTCGGGGAGAACGGGGCCCCACGGGTCCAAGGGGTCA GCGGGGACCTCGAGGTGCCACTGGGAAGTCTGGAGCTAAGGGAACATCAGGTGGTGATGGTCCTCACGGG CCGCCCGGAGAGAGGGGTCTTCCTGGACCGCAAGGCCCCAATGGATTCCCTGGCCCCAAAGGCCCTCCGG GCCCCGCAGGGAAAGACGGGCTGCCGGGACACCCAGGCCAGAGAGGAGAAGTGGGATTCCAAGGGAAGAC CGGCCCCCCAGGCCCGCCCGGAGTGGTGGGACCTCAGGGAGCAGCTGGGGAAAGCGGCCCCATGGGGGAG AGAGGTCACCCTGGCCCCCCAGGACCTCCTGGAGAGCAAGGGCTGCCCGGAACTGCTGGAAAAGATGGGA CCAAGGGTGACCCCGGGCCCCCCGGGGCCCCAGGGAAGGATGGCCCCGCTGGTCTGAGAGGCTTCCCAGG AGAGCGAGGCCTCCCGGGCACTGCTGGCGGACCTGGCTTGAAGGGAAATGAAGGCCCAGCTGGCCCTCCT GGCCCTGCAGGCTCTCCTGGCGAGCGAGGTGCAGCAGGATCAGGGGGCCCCATTGGTCCCCCTGGACGTC CAGGCCCACAGGGTCCCCCTGGGGCAGCAGGAGAGAAAGGCGTGCCGGGTGAGAAAGGCCCTATTGGTCC CACGGGTCGTGACGGGGTGCAGGGCCCTGTGGGGCTTCCTGGTCCCGCGGGACCCCCAGGCGTGGCAGGA GAGGATGGAGACAAGGGTGAGGTGGGCGACCCTGGGCAGAAGGGAACCAAAGGAAACAAGGGTGAGCATG GCCCTCCTGGCCCTCCTGGTCCCATCGGTCCTGTGGGGCAGCCTGGAGCTGCGGGAGCTGACGGCGAGCC CGGAGCCCGCGGACCCCAGGGACACTTTGGAGCCAAAGGTGACGAAGGAACAAGAGGGTTCAATGGGCCC CCTGGACCCATCGGCCTACAGGGCCTGCCAGGACCTTTTGGGGAAAAGGGAGAAACAGGAGACGTGGGGC CTATGGGACCCCCTGGCCCCCCAGGACCTCGAGGCCCCGCTGGACCCAATGGTGCTGATGGCCCACAAGG TCCCCCTGGAGGTGTCGGAAACTTGGGTCCCCCTGGAGAGAAGGGTGAACCAGGGGAGTCGGGGTCTCCA GGAGTCCAGGGTGAGCCGGGCGTCAAGGGTCCACGTGGAGAGCGTGGTGAGAAAGGAGAGTCTGGGCAGG CAGGAGAGGCTGGACCACCAGGGCCCAAAGGCCCTACAGGCGATGACGGCCCCAAGGGGAACCCTGGTCC CGTTGGCTTTCCTGGGGACCCTGGCCCCCCCGGAGAAGCTGGCCCAAGGGGCCAGGATGGTGCTAAGGGA AGCCAAGGCGAGGATGGCGAGCCGGGACAGCCTGGATCCCCCGGCCCCACCGGGGAGAACGGGCCCCCTG GACCTCTTGGAAAGCGGGGGGACCCTGGTGCTGTGGGGGCCCCAGGAAAGACGGGCCCTGTGGGCCCTGC AGGCCCAGCAGGGAAGCCTGGCCCCGATGGTCTTCGAGGGCTCCCTGGCTCAGTGGGTCAGCAAGGCCGC CCTGGAGCCACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGGACCCCCAGGGCTTCCTGGCCTCCGGGGTG ATGCTGGCGCCAAGGGGGAGAAGGGTCATCCAGGCCTCATCGGACTGATTGGGCCGACCGGAGAGCAAGG CGAGAAGGGTGACCGTGGCCTTCCTGGCCCTCAGGGCTCACCTGGACAAAAGGGAGAGACGGGTATCCCA GGAGCATCTGGTCCTATCGGTCCTGGAGGACCTCCTGGCCTGCCTGGACCGTCCGGCCCCAAAGGAGCTA AAGGAGCCACAGGCCCCGCTGGACCCAAAGGAGAGAAAGGTGTTCAGGGCCCTCCAGGACACCCGGGTCC CCCGGGCGAGGTGATCCAGCCACTGCCCATCCAGATGCCCAAGAAGACCCGGCGCTCTGTGGACGGAAGC AAACTGATGCAGGATGAGGAGGCCACACCCACTGGCGGTGCCCCGGGCAGTCCTGCAGGACTGGAGGAGA TCTTCGGTTCACTGGACTCTCTGCGGGAGGAGATCGAACAGATGAGGAGGCCGACAGGGACCCAGGACAG CCCTGCCCGCACCTGCCAGGACTTGAAGCTGTGCCACCCAGAGCTTCCCGATGGAGAGTACTGGGTTGAC CCCAACCAGGGCTGTGCCCGGGACGCCTTCCGGGTGTTCTGCAACTTTACAGCAGGAGGGGAGACTTGTG TCACACCCAGGGATGACGTCACACAGTTCTCCTACGTGGACTCTGAAGGCTCCCCAGTGGGCGTGGTCCA GCTCACCTTCCTGCGGCTGCTCAGCGTCTCTGCCCACCAGGATGTTTCCTACCCTTGCTCTGGGGTATCC CAGGACGGTCCCCTGAAACTCCGAGGGGCCAATGAGGATGAGCTGAGCCCTGAGACCAGCCCTTACGTCA AGGAGTTCAGAGATGGCTGTCAGACCCAGCAAGGCCGGACGGTGTTAGAGGTGCGCACGCCTGTGCTGGA GCAGCTGCCCGTGCTGGATGCTTCCTTCTCAGACCTGGGGGCCCCTACAAGACGGGGAGGGGTGCTTCTG GGGCCTGTCTGCTTCATGGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_212528
Insert Size	4854 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_212528.1, NP_997693.1
RefSeq Size	5951 bp
RefSeq ORF	4854 bp
Locus ID	294279

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.