Haus1 (NM_138864) Rat Tagged ORF Clone

CAT#: RR209269

  • TrueORF®

Haus1 (Myc-DDK-tagged ORF) - Rat coiled-coil domain containing 5 (Ccdc5), (10 ug)



  "NM_138864" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Ccdc5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR209269 representing NM_138864
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCTGGAGGAGAAAGCATCACAGGTCGCTGAGTGGTTGAAAAAGATATTTGGAGACCATCCCA
TTCCACAATATGAGATGAACGCACGGACGACAGAAATTTTATATCACCTTTCAGAACGCAACAGGGTCCG
GGACAGGGACGTCAACCTGGTAATAGAGGACTTAAGACAGAAAGCGAGTGAATATGAGTCGGAAGCCAAA
CGTCTTGAAGACTTTCTCATGGAGAGTGTGAATTTTTCCCCTGCCAATCTGTCAAACACTGGTTCCCGGT
TTCTGAATGCATTGGTGGACAGTGCAATTGCCCTGGAAATAAAAGATACGTCACTAGCAAGTTTTATCCC
CGCAGTGAACGATTTGACTTCTGACCTTTTTCGTACCAAATCCAAAAGTGAAGAAATCAAGCTTGAACTG
GGGAAACTTGAAAAGAATCTGACGGCAACGTTGGTGTTAGAAAAATGTCTCCGAGAAGACCTCAAGAAAG
CAGATGTGCACCTGTCTGCAGAAAGGGCCAAAGCCGAGGGCCGCCTCCAGAACATGGACTTCCTGAAAGC
CAAGGCGGCGGAGTTCCGGTTTGGGATCCGAGCTGCAGAGGAGCAACTTTCATCCAGAGGCATGGACGCG
TCCCTGTCTCATCGGTCACTAGTGGCACTTTCAGACAAACTTTCGGAATTAAAACAGCAGACTATCCCTC
TGAAGAAGAAATTGGAGTCCTATTTAGATCTAATGCCGAATCCATCTCTTGCTCAGGTGAAAATTGAAGA
GGCAAAGAGAGAATTAGATGCCATCGAGGCTGAACTCACAAAGAAAGTAGATATGATGGAGCTG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RR209269 representing NM_138864
Red=Cloning site Green=Tags(s)

MAALEEKASQVAEWLKKIFGDHPIPQYEMNARTTEILYHLSERNRVRDRDVNLVIEDLRQKASEYESEAK
RLEDFLMESVNFSPANLSNTGSRFLNALVDSAIALEIKDTSLASFIPAVNDLTSDLFRTKSKSEEIKLEL
GKLEKNLTATLVLEKCLREDLKKADVHLSAERAKAEGRLQNMDFLKAKAAEFRFGIRAAEEQLSSRGMDA
SLSHRSLVALSDKLSELKQQTIPLKKKLESYLDLMPNPSLAQVKIEEAKRELDAIEAELTKKVDMMEL

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_138864
ORF Size 834 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_138864.2, NP_620219.2
RefSeq Size 1598 bp
RefSeq ORF 837 bp
Locus ID 192228
UniProt ID Q9R0A8
MW 31.5 kDa
Gene Summary Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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