Wdr45 (NM_001013958) Rat Tagged ORF Clone
CAT#: RR209129
- TrueORF®
Wdr45 (Myc-DDK-tagged ORF) - Rat WD repeat domain 45 (Wdr45), (10 ug)
"NM_001013958" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209129 representing NM_001013958
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCAGCAGCCACTTCGAGGTGTGACCAGCCTCCATTTCAACCAAGACCAAAGCTGCTTTTGCTGTG CCATGGAGACAGGTGTACGGATCTACAATGTGGAACCACTGATGGAGAAAGGGCATCTTGACCACGAGCA GGTAGGCAGCGTGGGCCTTGTAGAGATGCTGCACCGATCCAACCTGCTGGCCCTGGTGGGCGGTGGTAGC AGCCCCAAGTTCTCCGAGATCTCGGTGCTGATCTGGGACGATGCCCGAGAAGGCAAGGACTCCAAGGACA AACTGGTGCTGGAGTTCACCTTCACCAAGCCAGTGCTAGCTGTGCGAATGCGCCATGACAAGATTGTGAT TGTGCTGAGGAACCGCATCTATGTGTACTCCTTCCCTGACAACCCTCGGAAGCTGTTTGAGTTTGACACT CGGGACAACCCCAAGGGGCTGTGTGACCTCTGCCCAAGCCTGGAGAAGCAGCTGCTTGTGTTTCCTGGAC ACAAGTGTGGAAGTCTGCAACTTGTGTCCAAGGAAAAGCTGGTAGAGCTTCGAAGAGGCACTGACCCTGC CACCCTATACTGCATTAACTTCAGCCATGACTCCTCCTTCCTCTGTGCTTCCAGTGACAAGGGCACTGTC CATATCTTTGCTCTTAAAGACACCCGCCTTAACCGCCGCTCTGCGTTGGCACGTGTGGGCAAAGTGGGAC CTATGATTGGGCAATACGTGGATTCTCAGTGGAGCCTGGCCAGCTTTACTGTGCCTGCTGAGTCAGCCTG CATCTGCGCCTTTGGTCGAAATACTTCCAAAAACGTCAATTCTGTAATCGCCATCTGCGTAGATGGGACC TTCCACAAATATGTCTTCACTCCTGATGGAAACTGCAACAGAGAGGCCTTTGACGTGTACCTTGACATCT GTGATGACGATGACTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209129 representing NM_001013958
Red=Cloning site Green=Tags(s) MTQQPLRGVTSLHFNQDQSCFCCAMETGVRIYNVEPLMEKGHLDHEQVGSVGLVEMLHRSNLLALVGGGS SPKFSEISVLIWDDAREGKDSKDKLVLEFTFTKPVLAVRMRHDKIVIVLRNRIYVYSFPDNPRKLFEFDT RDNPKGLCDLCPSLEKQLLVFPGHKCGSLQLVSKEKLVELRRGTDPATLYCINFSHDSSFLCASSDKGTV HIFALKDTRLNRRSALARVGKVGPMIGQYVDSQWSLASFTVPAESACICAFGRNTSKNVNSVIAICVDGT FHKYVFTPDGNCNREAFDVYLDICDDDDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001013958 |
ORF Size | 927 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001013958.1, NP_001013980.1 |
RefSeq Size | 1478 bp |
RefSeq ORF | 930 bp |
Locus ID | 302559 |
UniProt ID | Q5U2Y0 |
MW | 34.6 kDa |
Gene Summary | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed:21802374). Activated by the STK11/AMPK signaling pathway upon starvation, WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes. Probably recruited to membranes through its PtdIns3P activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209129 | Wdr45 (untagged ORF) - Rat WD repeat domain 45 (Wdr45), (10 ug) |
CNY 3,990.00 |
|
RR209129L3 | Lenti ORF clone of Wdr45 (Myc-DDK-tagged ORF) - Rat WD repeat domain 45 (Wdr45), (10 ug) |
CNY 6,080.00 |
|
RR209129L4 | Lenti ORF clone of Wdr45 (mGFP-tagged ORF) - Rat WD repeat domain 45 (Wdr45), (10 ug) |
CNY 6,650.00 |