Mfn1 (NM_138976) Rat Tagged ORF Clone
CAT#: RR209047
- TrueORF®
Mfn1 (Myc-DDK-tagged ORF) - Rat mitofusin 1 (Mfn1), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_138976" in other vectors (3)
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CNY 9,600.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Fzo1b |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209047 representing NM_138976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAAACGGTATCTCCACTGAAGCACTTCGTGCTGGCAAAGAAGGCCATCACTGCGATCTTCGGCC AGTTACTGGAGTTTGTTACTGAGGGCTCACATTTTGTTGAAGCAACATACAGGAACCCGGAACTTGATCG GATAGCAACCGAGGATGATCTGGTGGAGATACAGGGCTACAGAAACAAGCTCGCTGTCATTGGGGAGGTG CTGTCTCGGAGGCATATGAAAGTGGCATTTTTTGGCAGGACAAGTAGTGGCAAGAGCTCTGTCATCAACG CAATGCTGTGGGATAAAGTCCTCCCCAGCGGGATTGGTCACACAACCAACTGCTTCCTGAGTGTGGAGGG AACTGATGGAGATAAAGCCTACCTTATGACCGAAGGGTCAGATGAGAAGAAAAGCGTGAAGACTGTTAAT CAGCTGGCCCATGCCCTCCATATGGATAAAGACTTGAAAGCTGGTTGTCTTGTGCACGTATTTTGGCCCA AAGCAAAATGTGCCCTCTTGAGAGATGACCTGGTTTTAGTAGACAGCCCAGGTACAGATGTCACCACAGA GCTGGACATCTGGATTGATAAGTTCTGCCTTGATGCTGATGTTTTTGTTTTGGTGGCAAACTCGGAATCA ACGCTGATGAACACGGAGAAACATTTTTTCCATAAGGTGAATGAGCGGCTCTCCAAGCCAAACATTTTTA TCCTGAATAATCGTTGGGATGCTTCTGCTTCAGAGCCAGAATATATGGAGGATGTGCGCAGACAGCACAT GGAAAGATGTCTTCACTTCTTGGTAGAAGAGCTCAAGGTTGTGAGTCCTTTAGAAGCTCGGAATCGGATC TTTTTTGTTTCAGCCAAGGAAGTTCTCAACTCCAGAATGAATAAAGCCCAGGGGATGCCAGAAGGTGGAG GGGCACTTGCAGAAGGATTTCAGGCAAGATTACAGGAGTTTCAAAATTTTGAACAAACTTTTGAGGAGTG TATCTCGCAGTCAGCAGTGAAAACAAAGTTTGAACAGCACACTATCAGAGCTAAACAGATACTAGACACT GTGAAAAACATACTGGACTCAGTAAACGTGGCAGCAGCAGAGAAGAGGGTTTATTCAATGGAAGAGAGGG AAGACCAAATCGACAGACTGGACTTTATTCGAAACCAGATGAACCTTTTAACAATGGATGTTAAGAAGAA GATCAAGGAGGTCACGGAGGAGGTGGCAAACAAGGTTTCTTGTGCAATGACAGATGAGATTTGTCGCCTG TCTGTTTTGGTTGATGAGTTTTGTTCTGAGTTTCATCCTACCCCCAGTGTGCTGAAAGTGTATAAGAGTG AATTAAATAAGCACATAGAAGATGGCATGGGAAGAAATTTGGCTGATCGGTGCACCAGTGAAGTCAATGC CTCCATTCTTCAATCTCAGCAAGAAATCATTGAAAATTTGAAGCCACTACTTCCAGCTGGTATACAGAAT AAACTTCATACATTAATCCCTTGCAAAAAGTTTGATCTCAGCTATGATCTCAATTGCCACAAGCTGTGTT CGGATTTTCAAGAAGACATTGTGTTTCGGTTTTCCCTGGGCTGGTCTTCCCTTGTACATCGATTCCTGGG TTCTACGAATGCACAGAGGGTGCTGCTTGGGCTGTCAGAGCCTATCTTTCAGGTCCCTAGATCTTTAGCT TCAACTCCTACTGCTCCTTCTAACCCAGCAGCACCAGATAATGCAGCCCAGGAAGAGCTCATGATCACAC TGATCACAGGATTGGCATCCCTCACGTCTAGAACCTCCATGGGCATCATAGTTGTTGGGGGCGTGATTTG GAAAACAGTGGGCTGGAAACTCATCTCTGTCACCTTAAGTATGTATGGAGCTTTGTACCTTTATGAAAGA CTGACTTGGACTACTCGTGCGAAAGAGAGAGCGTTTAAGCAGCAGTTTGTAAACTATGCAACTGAAAAAC TGCAGATGATTGTGAAGTTCACAAGTGCAAACTGCAGCCACCAAGTCCAGCAAGAAATGGCCACCACTTT TGCTCGACTGTGCCAACAAGTTGATGTTACTCAGAAACATCTGGAAGAAGAAATTGCTAGATTATCCAAA GAGATTGACCAACTGGAGAAAATACAAAACAATTCAAAGCTCTTAAGAAATAAAGCTGTTCAACTTGAAC GTGAGCTGGAAAATTTTTCGAAGCAGTTTCTCCACCCGAGCAGTGGAGAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209047 representing NM_138976
Red=Cloning site Green=Tags(s) MAETVSPLKHFVLAKKAITAIFGQLLEFVTEGSHFVEATYRNPELDRIATEDDLVEIQGYRNKLAVIGEV LSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHTTNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVN QLAHALHMDKDLKAGCLVHVFWPKAKCALLRDDLVLVDSPGTDVTTELDIWIDKFCLDADVFVLVANSES TLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVSPLEARNRI FFVSAKEVLNSRMNKAQGMPEGGGALAEGFQARLQEFQNFEQTFEECISQSAVKTKFEQHTIRAKQILDT VKNILDSVNVAAAEKRVYSMEEREDQIDRLDFIRNQMNLLTMDVKKKIKEVTEEVANKVSCAMTDEICRL SVLVDEFCSEFHPTPSVLKVYKSELNKHIEDGMGRNLADRCTSEVNASILQSQQEIIENLKPLLPAGIQN KLHTLIPCKKFDLSYDLNCHKLCSDFQEDIVFRFSLGWSSLVHRFLGSTNAQRVLLGLSEPIFQVPRSLA STPTAPSNPAAPDNAAQEELMITLITGLASLTSRTSMGIIVVGGVIWKTVGWKLISVTLSMYGALYLYER LTWTTRAKERAFKQQFVNYATEKLQMIVKFTSANCSHQVQQEMATTFARLCQQVDVTQKHLEEEIARLSK EIDQLEKIQNNSKLLRNKAVQLERELENFSKQFLHPSSGES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138976 |
ORF Size | 2223 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138976.1, NP_620432.1 |
RefSeq Size | 2226 bp |
RefSeq ORF | 2226 bp |
Locus ID | 192647 |
UniProt ID | Q8R4Z9 |
MW | 83.8 kDa |
Gene Summary | mouse homolog is involved in regulation of mitochondrial fusion and is essential for embryonic development [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209047 | Mfn1 (untagged ORF) - Rat mitofusin 1 (Mfn1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 9,600.00 |
|
RR209047L3 | Lenti ORF clone of Mfn1 (Myc-DDK-tagged ORF) - Rat mitofusin 1 (Mfn1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 11,310.00 |
|
RR209047L4 | Lenti ORF clone of Mfn1 (mGFP-tagged ORF) - Rat mitofusin 1 (Mfn1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 12,450.00 |