Synrg (NM_053419) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ap1gbp1; Syng
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209031 representing NM_053419 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCGGCCGGGAGCCGGAGCCAGCGGCGCGGCGGGGGCGGGCACAGGGCCCGGCGGGGCAGGCA GCTTCATGTTTCCTGTCGCAGGTGGCATGAGACCCCCTCAAGGCCTGATTCCAATGCAGCAGCAAGGATT TCCTATGGTCTCTGTCATGCAGCCCAATATGCAAGGCATGATGGGAATGAATTACAGCTCTCAGATGTCC CAGGGACCCATTGCAATGCAGGCAGGAATACCAATGGGGCCAATGCCAGCAGCGGGTGTACCTTTCCTGG GTCAGCCACCTTTTCTGGGCATGCGTCCTGCAGCCCCACAGTACACTCCAGACATGCAGAAGCAGTTTGC GGAAGAGCAACAAAAACGATTTGAACAACAACAAAAGCTCTTAGAAGAAGAAAGAAAACGACGTCAGTTT GAAGAGCAAAAACAAAAGCTCAGACTTCTGAGTAGCGTGAAACCCAAGACAGGAGAGAAGAACAGAGACG ACGCTTTGGAAGCCATCAAAGGAAACTTGGATGGGTTTTCCAGAGATGCTAAGATGCACCCCACTCCCGC ATCTCACCCAAAGAAACCAGACTGCCCCACATCATCTCATTCTACTAAAACTGTCTCCCCGTCATCTGCC TTTCTTGGTGAAGATGAATTCAGTGGCTTTATGCAGGGGCCTGTTGAGCTTCCCACTTGTGGGCCTTCTT CCACAGCCCAGCCCTTTCAGTCTTTCCTCCCCAGCACCCCACTCGGCCAGTTACACACACAGAAGGCTGG GGCACAGCCTCTACCTCCAGGTCAGGCTCCAGTGTCTTTTGCCGTACATGGTGTCCATGGGCAAATTCCT TGTTTATCTGCTGCTTCAGCTTCCCACAGTATGCAGAAAGCAGGCCCTTCCTTGGAAGAGAAGCTCCTAG TATCTTGTGATATAAGTGCATCTGGGCAGGAACACATTAAATTAAGCTCTCCTGAAGCTGGGCACAGAGC CGTAGTCCCAGGTTCCAGTAAGAACTCTCCCGGTTTAATGGCCCATAACGGGGGTGCTGTAGATGGATGT GTGAGTGGTCCCACCACTGCAGTCGCAGAAAAGACTTCAGATCAAAACCTGTCAAAGGAAGAGAGTGGTG TGGGCGTGTTTCCCTCTCAGGATCCTGTTCAGCCCAGAATGCCTCCTTGGATTTACAATGAGAGTTTGGT TCCAGATGCTTATAAGAAAATTTTAGAAACCACAATGACTCCAACCGGAATAGACACTGCTAAACTTTAT CCCATTCTGATGTCATCTGGACTTCCCAGGGAAACTCTTGGACAGATATGGGCCTTAGCTAATCGAACTA CGCCTGGCAAGCTCACTAAGGAGGAGCTTTACACCGTTCTCGCCATGGTAGCAGTAACACAGAGAGGTGT TCCTGCCATGAGCCCTGATACTTTGAACCAGTTCCCTGCGGCTCCCATTCCTACCTTAAGCGGCTTTCCT ATGACTCTGCCTACCCCAGTGAGCCAGCCAACAGCGATGACCTCTGGCCCCGCAGGCTCCATACCCCTCA GCCTTGGACAGCCCATCATGGGCATTAACCTTGTTGGACCAGTGGGTGGAGCTGCAGCGCCAACTTCCAG TGGCTTCATGCCAGCCTACCCCTCAAACCAGGTGGGAAAGACAGAAGAAGATGACTTCCAGGATTTTCAA GATGCTTCGAAGTCAGGATCCATCGATGATTCTTTCACTGACTTCCAAGAGGTGCCTGCGTCCTCAAAAA CCAGTAATTCCCAGCATGGAAACAGTGCTCCCTCACTCTTGATCCCACTTCCTGGAACTAAAGCCTCCAC AGACAAATATGCAGTGTTTAAAGGGATTTCTGCTGAGAAGCCCTCTGAAAACCCTGCTTCATTTGGAGAG TCCGGTGATAAATACAGTGCTTTCAGGGAACTCGAGCCAACAGCAGACAGTAAGCCTTTAGGAGAGAGCT TTGCGGAGTTCAGATCCACAGGAACCGATGATGGCTTCACTGACTTCAAGACTGCTGATAGTGTGTCACC CCTAGAGCCACCAACAAAAGACTCTTTTCCATCGGCCTTTGCCTCAGGAGCTGCACAGCAGACACAGACA CAGGTGAAAACCCCTCTGAACTTAGCAGACCTAGATATGTTCTCCTCAGTGGATTGCAGTGGGGAGAAAC CAGTGCCCTTTTCAGCTGCATTTAGCACCTCAAAGTCAGTTTCCTCAAGACCTCAGCCGGCGGGTTCTGC AGCAGCCCCAGCATCACTGGCATCCACTAAAGCGTCCAGTTTGGCAGATGATTTTGGAGAGTTCAACCTC TTTGGGGAATATTCGAACCCTGCGTCTGTTGGGGAGCAGGACGACTTTGCAGATTTCATGGCTTTCGGTA ACAGCTCCATCCCCTCTGAGCCAAAAGCAGATGACAAGTATGAAGCCCTCAGAGAAGAAGGGAGCCCCGG CGCCTTGTCCACCAGCACTGTGGAGGGTGCACACAACCCACCTGTTTCGTCATCCAAGTACGACGTCTTC AAACAGCTTTCCCTAGAGGGAGCTGGACTGGCCATCGAGGAGTTCAAAGAGAACACTCCTTCCACAAAAA GTGACGGTGACTTTGCTGACTTCCACTCCAGTAAATTCTCATCCACGAGCTCAGACAAATCCCTGGGAGA GAAAGCAGTGGCTTTCAGACATGCCAAAGAAGACTCCGCCTCGGTGAAGTCTTTAGACCTCCCCTCCATC GGTGGCAGCAGTGTTGGCAAGGAGGACTCTGAAGATGCGCTTTCTGTTCAGTTTGACATGAAGTTGGCTG ATGTAGGAGGAGATCTTAAGCATGTCATGTCTGATAGCTCTTTGGATTTACCAACAGTTAGTGGCCAACA TCCTCCTGCCGCAGGATCAGCCTTGGCCAGTGAGGACGCTCTTCCAGAGACTCCCTTCCCAGCTTTTGCC AGCTTTAAAGACATGATGCCCCAGACCACTGAGCAGAAAGAGTATGAAAGTGGGGACTTCCAGGATTTCA CAAGGCAGGACATGCCCATGGTGGACCGGAGCCAAGAGAACACATGTCCCAGCCCAGCTTCCAGTGTGGC CTCGCACGAAACCCCCAAGGAGGGTGCAGACGACTTTGGAGAATTTCAAAGTGAAAAGCCCAAAATCAGC AAGTTTGACTTTTTAGTAGCTAATTCACAGAGCAAAATGAAGTCTAGTGAAGAAATGATCAAAAGTGAGC TGGCAACCTTTGACCTTTCTGTTCAAGGATCACACAAGAGGAGCTTGAGCCTTGGGGATAAAGAAATAAG CAGGTCCTCTCCTTCCCCGGCCCTGGAGCAGCCCTTCAGAGACCGGTCCAACACTCTGAGTGAGAGAGCT GCACTGCCCGTCATCCGAGACAAGTACAAGGATCTGACTGGCGAAGTGGAGGAAAATGAGAGATACGCGT ATGAGTGGCAGAGATGCCTGGGGAGCGCCCTGGATGTCATTAAGAAGGCAAACGACACCTTAAACGGCAT TAGCAGCAGTGCTGTCTGCACAGAAGTTATCCAGTCAGCCCAAGGCATGGAGTATCTCCTAGGCGTGGTG GAAGTGTACCGAGTAACCAAGCGCGTGGAGCTGGGGATCAAAGCCACTGCTGTGTGTAGTGAGAAACTCC AGCAGTTGCTGAAGGACATCGATAAAGTGTGGAACAACTTAATCGGCTTCATGTCACTTACCACACTCAC GCCAGATGAGAATTCACTGGATTTTTCCTCCTGTATGTTACGGCCTGGGATCAAAAACGCCCAGGAGCTG GCTTGTGGAGTGTGTCTCCTAAATGTGGACTCCAGAAGCCGGAAAGAAGAGACACCTGCAGAAGAACAGC CTAAAAAAGCATTCAACTCAGAAACAGACAGTTTCAAGCTGGCCTATGGGGGACATCAGTACCACGCCAG CTGTGCCAACTTCTGGATCAACTGTGTTGAACCCAAGCCTCCCGGCCTCCTCTTGCCAGATCTGCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209031 representing NM_053419 Red=Cloning site Green=Tags(s) MALRPGAGASGAAGAGTGPGGAGSFMFPVAGGMRPPQGLIPMQQQGFPMVSVMQPNMQGMMGMNYSSQMS QGPIAMQAGIPMGPMPAAGVPFLGQPPFLGMRPAAPQYTPDMQKQFAEEQQKRFEQQQKLLEEERKRRQF EEQKQKLRLLSSVKPKTGEKNRDDALEAIKGNLDGFSRDAKMHPTPASHPKKPDCPTSSHSTKTVSPSSA FLGEDEFSGFMQGPVELPTCGPSSTAQPFQSFLPSTPLGQLHTQKAGAQPLPPGQAPVSFAVHGVHGQIP CLSAASASHSMQKAGPSLEEKLLVSCDISASGQEHIKLSSPEAGHRAVVPGSSKNSPGLMAHNGGAVDGC VSGPTTAVAEKTSDQNLSKEESGVGVFPSQDPVQPRMPPWIYNESLVPDAYKKILETTMTPTGIDTAKLY PILMSSGLPRETLGQIWALANRTTPGKLTKEELYTVLAMVAVTQRGVPAMSPDTLNQFPAAPIPTLSGFP MTLPTPVSQPTAMTSGPAGSIPLSLGQPIMGINLVGPVGGAAAPTSSGFMPAYPSNQVGKTEEDDFQDFQ DASKSGSIDDSFTDFQEVPASSKTSNSQHGNSAPSLLIPLPGTKASTDKYAVFKGISAEKPSENPASFGE SGDKYSAFRELEPTADSKPLGESFAEFRSTGTDDGFTDFKTADSVSPLEPPTKDSFPSAFASGAAQQTQT QVKTPLNLADLDMFSSVDCSGEKPVPFSAAFSTSKSVSSRPQPAGSAAAPASLASTKASSLADDFGEFNL FGEYSNPASVGEQDDFADFMAFGNSSIPSEPKADDKYEALREEGSPGALSTSTVEGAHNPPVSSSKYDVF KQLSLEGAGLAIEEFKENTPSTKSDGDFADFHSSKFSSTSSDKSLGEKAVAFRHAKEDSASVKSLDLPSI GGSSVGKEDSEDALSVQFDMKLADVGGDLKHVMSDSSLDLPTVSGQHPPAAGSALASEDALPETPFPAFA SFKDMMPQTTEQKEYESGDFQDFTRQDMPMVDRSQENTCPSPASSVASHETPKEGADDFGEFQSEKPKIS KFDFLVANSQSKMKSSEEMIKSELATFDLSVQGSHKRSLSLGDKEISRSSPSPALEQPFRDRSNTLSERA ALPVIRDKYKDLTGEVEENERYAYEWQRCLGSALDVIKKANDTLNGISSSAVCTEVIQSAQGMEYLLGVV EVYRVTKRVELGIKATAVCSEKLQQLLKDIDKVWNNLIGFMSLTTLTPDENSLDFSSCMLRPGIKNAQEL ACGVCLLNVDSRSRKEETPAEEQPKKAFNSETDSFKLAYGGHQYHASCANFWINCVEPKPPGLLLPDLL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053419
ORF Size	3987 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053419.1, NP_445871.1
RefSeq Size	3990 bp
RefSeq ORF	3990 bp
Locus ID	84479
UniProt ID	Q9JKC9
MW	141.4 kDa
Gene Summary	may link the AP1 adaptor complex to other proteins [RGD, Feb 2006]

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