Sema5b (NM_001107091) Rat Tagged ORF Clone
CAT#: RR208999
- TrueORF®
Sema5b (Myc-DDK-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), (10 ug)
"NM_001107091" in other vectors (3)
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CNY 16,720.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208999 representing NM_001107091
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGTCCCAGGACCCCTGGCTCTCTCGCTGCTGCTGCCTAGCTTCACCCTGCTGCTGTCCCACCTCT CCACCTCCCAGGACATCACCAGTGAGCCCAGCAGTGAGCAGCAGCTGTGCACCCGGAGGGAGCACCCCAT CGTGGCCTTCGAAGATCTGAAGCCGTGGGTGTCTAACTTCACCTACCCTGGAGTCCGGGATTTCTCTCAG CTTGCTCTGGATCCCTCCAGGAACCAGCTCATCGTGGGAGCCAGGAACTACCTCTTCAGACTCAGCCTTG CCAATGTTTCCCTCCTTCAGGCTACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAGAGCAA AGGGAAGACTGAGGAGGAATGTCAGAACTACGTTCGAGTCCTGATTGTTTCCGGCCGGAAGGTGTTCATG TGCGGTACCAATGCCTTTTCCCCTGTGTGCTCCAGCAGACAGGTGGGGAACCTCAGCCGGGCTATTGAGA AGATCAATGGCGTGGCCCGCTGCCCCTATGACCCACGCCACAACTCTACAGCCGTGATCTCCTCCCAGGG GGAGCTCTACGCAGCCACCGTCATTGACTTCTCCGGTCGGGACCCGGCCATCTACCGTAGCCTGGGCAGT GGGCCGCCGCTTCGCACTGCCCAGTATAACTCTAAATGGCTCAATGAGCCAAATTTTGTGGCAGCCTTTG ACATTGGGCTGTTTGCGTATTTCTTCCTTCGGGAGAATGCAGTGGAGCATGACTGTGGACGCACTGTGTA CTCTCGGGTGGCCCGAGTGTGCAAGAATGATGTAGGGGGTCGTTTCCTGCTGGAGGACACATGGACCACA TTCATGAAGGCCCGGCTCAACTGCTCCCGCCCAGGGGAGGTCCCCTTCTACTATAACGAGCTGCAGAGTG CCTTCCATCTGCCCGAACAGGACCTCATCTATGGCGTCTTCACCACTAACGTAAACAGCATTGCAGCTTC TGCCGTCTGCGCCTTCAACCTCAGTGCCATCTCCAAGGCTTTCAATGGCCCATTTCGTTACCAGGAAAAC CCCAGGGCTGCCTGGCTGCCTATCGCCAACCCCATCCCCAATTTCCAGTGTGGCACCCTGCCGGAGACTG GCCCTAACGAGAACCTCACGGAGCGCAGCCTGCAAGATGCACAGAGGCTCTTCCTGATGAGCGAAGCTGT GCAGCCGGTGACGCCAGAGCCCTGTGTCACCCAGGACAGCGTCCGCTTCTCACATCTTGTGGTAGACCTT GTGCAAGCTAAGGACACACTGTACCACGTACTCTACATAGGCACTGAGTCGGGTACCATCCTGAAAGCGC TGTCCACCGCCAGCCGCAGTCTGCGTGGCTGCTACCTAGAGGAACTGCATGTGCTGCCCCCTGGGCGCCT TGAACCTCTGCGGAGCCTGCGCATCCTGCACAGCGCGCGTGCGCTCTACGTGGGATTGAGCGACCGGGTG CTGCGGGTCCCGCTGGAGAGGTGCTCGGCCTACCTTAGCCAGGGGGCATGCCTGGGAGCACGGGACCCAT ACTGCGGCTGGGATGGGAAGCGGCAACTTTGCAGCACGCTTGAGGACAGTTCCAACATGAGCCTGTGGAT CCAGAACATCACAGCCTGTCCTGTACGAAATGTGACACGGGACGGGGGCTTCGGCCCATGGTCACCATGG AAACCATGTGAGCACTTAGATGGAGACAACTCGGGTTCTTGCCTATGCCGGGCCAGATCCTGTGACTCCC CAAGGCCCCGCTGTGGGGGCCTCGAATGCCTGGGGCCAGCCATCCATATCGCCAATTGCTCCAGGAATGG GGCGTGGACCGCATGGTCATCGTGGGCTCAGTGCAGCACGTCCTGTGGGATCGGCTTCCAGGTCCGTCAG CGAAGTTGCAGCAACCCAGCGCCCCGCCATGGGGGCCGCATCTGCGTGGGCAAGAGCCGGGAGGAGCGGT TCTGTAATGAAAATACGCCTTGCCCGGTGCCCATCTTCTGGGCTTCCTGGGGCTCGTGGAGCAAGTGCAG CAGCAACTGTGGGGGTGGCGTGCAGTCGCGCCGTCGTTCTTGCGAGAATGGCAACTCGTGCCCGGGTTGC GGCGTGGAGTTCAAGACCTGCAACCCCGAGGCCTGCCCAGAAGTGCGACGCAACACACCCTGGACGCCCT GGCTACCCGTGAATGTGACGCAGGGTGGCGCGCGCCAGGAGCAGCGATTCCGCTTCACCTGCCGCGCGCC GCTGCCGGACCCGCACGGTCTGCAGTTCGGCAAGAGGAGGACGGAGACCAGGACCTGCCCGGCTGATGGC ACCGGAGCATGCGACACTGACGCCCTAGTGGAGGATCTGCTGCGCAGCGGGAGCACGTCACCACACACTC TGAGTGGAGGCTGGGCCACCTGGGGCCTGTGGTCATCCTGCTCCCGGGACTGCGAGCTGGGCTTCCGCGT ACGTAAGAGAACTTGTACCAACCCGGAGCCCCGCAACGGGGGCTTGCCCTGCGTGGGAGATGCTGCAGAG TACCAGGACTGCAACCCTCAGGCTTGCCCAGTGCGGGGTGCCTGGTCCTGCTGGACTGCATGGTCCCAGT GCTCAGCATCCTGCGGTGGTGGCCACTATCAACGCACCCGTTCCTGCACCAGCCCCGCACCGTCCCCAGG TGAGGACATCTGCCTCGGCCTGCACACGGAGGAGGCACTATGTGCTACACAGGCCTGCCCAGAAGGCTGG TCACTGTGGTCTGAGTGGGGTGTCTGCACGGAGGATGGGGCCCAGAGCAGGAGCAGGAGCTGTGAGGAGC TTGTCCCAGGGCCAGGTGCCTGTGTTGGCAACAGCAGCCAGAGCCGGCCCTGCCCCTACAGTGAGATTCC TGTCATCCTGCCTGCTTCCAGTGTGGAGGAGACTGCCAGCTGTGGAGGGTTCAGTCTCATCCACCTGATA GTCACTGGCGTGTCCTGCTTCCTGGGATCTGGGCTACTGACCTTGGCAGTGTACCTGTCCTGCCAGCACT GCCAGCGCCAGTCTCAGGAGTCCACACTTGTCCATCCCGCCACCCCTAACCACTTGCACTACAAAAGCGG GGGCACTCCCAAGAATGAGAAGTACACCCCCATGGAATTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208999 representing NM_001107091
Red=Cloning site Green=Tags(s) MVVPGPLALSLLLPSFTLLLSHLSTSQDITSEPSSEQQLCTRREHPIVAFEDLKPWVSNFTYPGVRDFSQ LALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFM CGTNAFSPVCSSRQVGNLSRAIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGS GPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTT FMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQEN PRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDL VQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALYVGLSDRV LRVPLERCSAYLSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITACPVRNVTRDGGFGPWSPW KPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPAIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQ RSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQSRRRSCENGNSCPGC GVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGKRRTETRTCPADG TGACDTDALVEDLLRSGSTSPHTLSGGWATWGLWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAE YQDCNPQACPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGW SLWSEWGVCTEDGAQSRSRSCEELVPGPGACVGNSSQSRPCPYSEIPVILPASSVEETASCGGFSLIHLI VTGVSCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKSGGTPKNEKYTPMEFK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001107091 |
ORF Size | 3123 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001107091.1, NP_001100561.1 |
RefSeq Size | 3692 bp |
RefSeq ORF | 3126 bp |
Locus ID | 303901 |
MW | 114.2 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208999 | Sema5b (untagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), (10 ug) |
CNY 16,720.00 |
|
RR208999L3 | Lenti ORF clone of Sema5b (Myc-DDK-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), (10 ug) |
CNY 18,340.00 |
|
RR208999L4 | Lenti ORF clone of Sema5b (mGFP-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), (10 ug) |
CNY 20,140.00 |