Nrxn1 (NM_021767) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nrxn1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208761 representing NM_021767 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACGGCGCTGGTCCAGCATGGGGGCTGCTGTCTCCTCTGCCTGTCGCTGCTGCTGTTGGGCTGCT GGGCAGAGCTGGGCAGTGGGCTGGAGTTCCCAGGCGCTGAGGGCCAGTGGACGCGCTTCCCCAAGTGGAA CGCGTGCTGCGAGAGTGAGATGAGCTTCCAGCTGAAGACACGCAGCGCCCGCGGCCTCGTGCTCTACTTC GACGACGAGGGCTTCTGCGACTTCCTCGAGCTCATCCTGACACGCGGCGGCCGCTTGCAGCTCAGCTTCT CCATCTTCTGCGCCGAGCCCGCCACGCTGTTGGCCGACACGCCAGTCAACGACGGCGCCTGGCACAGCGT GCGCATCCGCCGCCAGTTCCGCAACACCACTCTCTACATCGACCGCGCTGAGGCCAAGTGGGTGGAGGTC AAGTCCAAGCGCAGGGACATGACGGTGTTCAGTGGGCTGTTCGTGGGCGGCCTGCCGCCTGAACTGCGAG CCGCTGCGCTCAAGCTCACCCTGGCCTCGGTGCGTGAGCGGGAGCCCTTCAAGGGCTGGATCCGTGATGT CAGGGTCAACTCGTCCCAGGCGCTGCCGGTGGACGGCAGCGAGGTCAAGCTGGACGAAGAGCCGCCCAAT AGCGGGGGCGGGAGCCCGTGCGAGGCGGGCGATGAGGGCGACGGCGGCGTGTGCCTCAACGGGGGCGTGT GCTCCGTGGTCGACGACCAGGCCGTGTGTGACTGCTCGAGGACCGGTTTCCGTGGCAAAGACTGCAGCCA AGAAGACAACAATGTAGAAGGTCTGGCGCACCTGATGATGGGCGACCAAGGTAAAAGTAAAGGAAAAGAA GAGTATATTGCCACATTCAAGGGATCTGAGTACTTCTGCTACGACTTATCCCAAAACCCCATTCAAAGCA GCAGCGACGAAATAACTCTGTCCTTTAAAACTCTTCAGAGGAATGGACTGATGCTTCACACAGGGAAATC GGCTGATTATGTCAACCTTGCCTTGAAAAATGGAGCTGTCTCTCTGGTCATTAATTTGGGATCAGGGGCC TTTGAAGCGCTAGTAGAGCCCGTGAATGGAAAGTTTAATGATAATGCCTGGCATGATGTGAAAGTCACCA GGAATCTGCGTCAGCACTCAGGCATTGGACACGCTATGGTGACAATATCAGTGGATGGGATTCTTACCAC AACGGGCTACACGCAAGAAGATTACACCATGCTGGGGTCTGATGACTTTTTCTATGTTGGAGGCAGCCCC AGCACAGCCGACCTTCCAGGGTCACCAGTCAGTAACAACTTTATGGGCTGTCTCAAAGAGGTCGTATATA AAAATAATGATGTAAGACTGGAGTTATCGCGACTTGCCAAGCAAGGAGATCCTAAGATGAAGATCCATGG TGTGGTGGCTTTTAAGTGTGAAAATGTGGCAACCTTAGACCCAATCACTTTTGAGACCCCAGAATCTTTC ATTTCTTTGCCTAAGTGGAATGCAAAGAAAACAGGCTCAATATCATTTGATTTCCGTACAACGGAGCCAA ATGGCCTCATCTTGTTTAGCCATGGCAAGCCAAGACATCAGAAGGATGCCAAGCACCCGCAGATGATTAA GGTTGACTTTTTTGCTATTGAAATGCTGGATGGCCACTTGTACCTCCTCTTAGACATGGGATCAGGTACT ATAAAAATCAAAGCCCTTCAGAAGAAAGTCAATGATGGAGAATGGTATCACGTGGATTTCCAGCGAGATG GACGGTCAGGTACCATTTCTGTCAACACACTTCGTACTCCCTACACAGCTCCCGGTGAGAGTGAGATCCT AGACCTCGATGATGAGTTGTATCTGGGTGGCTTGCCAGAAAATAAGGCTGGCCTGGTCTTCCCCACTGAG GTGTGGACTGCTCTGCTCAACTATGGCTACGTAGGCTGCATCCGGGATCTATTCATTGATGGCCAGAGCA AAGATATCCGGCAGATGGCGGAAATTCAGAGTACTGCTGGAGTGAAGCCATCCTGCTCAAGGGAGACTGC AAAACCATGCCTTAGCAATCCTTGCAAAAACAATGGCATGTGCAGGGATGGCTGGAACAGATACGTCTGT GATTGCTCTGGAACAGGCTATCTCGGCAGGTCCTGTGAGAGAGAGGCAACGGTTTTGAGCTACGATGGGA GTATGTTTATGAAAATTCAGCTCCCTGTGGTGATGCACACTGAGGCCGAGGACGTTTCCTTACGGTTCAG ATCCCAGCGTGCGTACGGCATTCTGATGGCGACCACTTCTAGAGACTCCGCAGATACCCTTCGACTGGAG CTAGATGCAGGGCGCGTGAAACTCACGGTCAATCTAGATTGTATCAGGATTAACTGTAATTCCAGCAAAG GTCCTGAGACCCTTTTTGCCGGCTATAACCTCAATGATAATGAATGGCACACAGTACGTGTGGTTCGGCG AGGAAAAAGTTTAAAGCTAACCGTGGATGATCAACAAGCCATGACAGGCCAAATGGCAGGTGATCATACA AGGCTGGAGTTTCATAACATAGAGACAGGCATCATCACAGAACGACGCTATCTTTCTTCTGTCCCCTCCA ACTTCATTGGGCACCTGCAAAGCCTCACATTTAATGGAATGGCATACATTGACTTGTGCAAAAATGGTGA TATAGATTATTGTGAACTCAATGCCAGGTTTGGATTCAGGAACATCATCGCGGATCCTGTCACCTTCAAG ACCAAATCAAGCTATGTTGCCTTAGCTACATTGCAAGCCTACACTTCTATGCATCTATTTTTCCAATTCA AGACAACATCCCTAGATGGACTAATTCTGTATAACAGTGGGGATGGAAATGACTTTATTGTGGTTGAATT AGTTAAAGGGTACTTACATTATGTGTTTGACTTGGGAAATGGTGCTAACCTCATCAAAGGGAGCTCAAAC AAACCACTCAACGACAATCAGTGGCACAATGTGATGATTTCAAGGGACACCAGCAATCTCCACACAGTAA AGATCGACACAAAAATCACAACACAAATCACTGCGGGAGCCAGAAACTTAGACCTCAAGAGTGACTTGTA TATTGGAGGAGTGGCTAAAGAAACATACAAATCGTTGCCGAAACTGGTCCATGCTAAAGAAGGCTTTCAA GGTTGCCTGGCATCTGTGGACTTGAATGGCAGGCTTCCAGACCTCATATCTGATGCTCTTTTCTGCAATG GGCAAATTGAGAGAGGATGCGAAGGGCCCAGCACAACCTGTCAAGAGGACTCATGTTCAAATCAAGGCGT GTGCTTGCAGCAATGGGATGGCTTTAGCTGTGACTGTAGCATGACTTCCTTCAGTGGCCCGCTCTGCAAT GACCCTGGGACGACGTATATCTTTAGCAAAGGTGGTGGACAAATTACACATAAGTGGCCTCCTAATGACC GACCCAGTACACGAGCAGACAGGCTGGCCATCGGGTTTAGCACTGTTCAGAAGGAAGCAGTGTTGGTGCG TGTGGACAGTTCCTCAGGCCTGGGTGACTACCTTGAGCTGCACATACACCAAGGAAAAATTGGAGTTAAA TTTAATGTTGGGACAGATGACATCGCCATTGAGGAGTCCAATGCAATCATTAATGATGGAAAATACCATG TAGTGCGTTTCACGAGGAGTGGTGGCAATGCCACGTTACAGGTGGACAGCTGGCCAGTCATCGAACGATA CCCTGCAGGAAACAATGATAACGAGCGCCTGGCGATTGCTAGACAGCGAATTCCATATCGACTTGGTCGA GTAGTTGATGAATGGCTACTCGACAAAGGGCGTCAGCTCACAATCTTCAATAGCCAAGCAACCATAATAA TTGGCGGGAAAGAGCAGGGCCAGCCCTTCCAGGGCCAGCTCTCTGGGCTTTACTACAATGGCTTGAAAGT TCTGAATATGGCGGCAGAAAACGATGCCAACATCGCCATAGTGGGAAATGTGAGGCTGGTCGGTGAAGTG CCTTCCTCTATGACAACTGAGTCGACAGCCACTGCCATGCAGTCCGAGATGTCCACCTCAATCATGGAGA CCACCACAACCCTGGCTACCAGCACAGCTCGACGAGGAAAGCCCCCCACAAAGGAGCCTATCAGCCAGAC CACGGATGATATCCTTGTGGCCTCGGCAGAGTGTCCCAGCGACGATGAGGACATTGACCCCTGTGAGCCG AGCTCAGGTGGGTTAGCCAACCCCACCCGAGTGGGCGGCCGCGAACCATACCCAGGCTCGGCAGAGGTGA TCCGGGAGTCTAGCAGTACCACTGGCATGGTGGTGGGGATTGTCGCAGCAGCAGCTCTGTGCATCCTCAT CCTCCTCTATGCCATGTACAAGTACAGAAACCGGGATGAAGGCTCATATCACGTGGATGAGAGTCGAAAC TACATCAGTAACTCAGCACAGTCCAATGGGGCTGTGGTCAAGGAGAAGCAGCCCAGCAGTGCGAAAAGCG CCAACAAAAACAAGAAGAACAAGGATAAGGAGTATTATGTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208761 representing NM_021767 Red=Cloning site Green=Tags(s) MGTALVQHGGCCLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFPKWNACCESEMSFQLKTRSARGLVLYF DDEGFCDFLELILTRGGRLQLSFSIFCAEPATLLADTPVNDGAWHSVRIRRQFRNTTLYIDRAEAKWVEV KSKRRDMTVFSGLFVGGLPPELRAAALKLTLASVREREPFKGWIRDVRVNSSQALPVDGSEVKLDEEPPN SGGGSPCEAGDEGDGGVCLNGGVCSVVDDQAVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKSKGKE EYIATFKGSEYFCYDLSQNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGA FEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSP STADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKQGDPKMKIHGVVAFKCENVATLDPITFETPESF ISLPKWNAKKTGSISFDFRTTEPNGLILFSHGKPRHQKDAKHPQMIKVDFFAIEMLDGHLYLLLDMGSGT IKIKALQKKVNDGEWYHVDFQRDGRSGTISVNTLRTPYTAPGESEILDLDDELYLGGLPENKAGLVFPTE VWTALLNYGYVGCIRDLFIDGQSKDIRQMAEIQSTAGVKPSCSRETAKPCLSNPCKNNGMCRDGWNRYVC DCSGTGYLGRSCEREATVLSYDGSMFMKIQLPVVMHTEAEDVSLRFRSQRAYGILMATTSRDSADTLRLE LDAGRVKLTVNLDCIRINCNSSKGPETLFAGYNLNDNEWHTVRVVRRGKSLKLTVDDQQAMTGQMAGDHT RLEFHNIETGIITERRYLSSVPSNFIGHLQSLTFNGMAYIDLCKNGDIDYCELNARFGFRNIIADPVTFK TKSSYVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLHYVFDLGNGANLIKGSSN KPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLKSDLYIGGVAKETYKSLPKLVHAKEGFQ GCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCN DPGTTYIFSKGGGQITHKWPPNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVK FNVGTDDIAIEESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGNNDNERLAIARQRIPYRLGR VVDEWLLDKGRQLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEV PSSMTTESTATAMQSEMSTSIMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECPSDDEDIDPCEP SSGGLANPTRVGGREPYPGSAEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRN YISNSAQSNGAVVKEKQPSSAKSANKNKKNKDKEYYV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021767
ORF Size	4521 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021767.2, NP_068535.2
RefSeq Size	5609 bp
RefSeq ORF	4524 bp
Locus ID	60391
UniProt ID	Q63372
MW	165.4 kDa
Gene Summary	This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are synaptic transmembrane receptors that bind endogenous ligands that include neuroligins, dystroglycan, and neurexophilins. Neurexin complexes are required for efficient neurotransmission and are involved in synaptogenesis. In vertebrates, alternate promoter usage results in multiple isoform classes, of which the alpha and beta classes are the best characterized. In humans, allelic variants in this gene are associated with Pitt-Hopkins-like syndrome-2, while deletions have been associated with autism and schizophrenia. Mouse knockouts display decreased spontaneous and evoked vesicle release resulting in impaired synaptic transmission. In addition, knockout mice show altered social approach, reduced social investigation, reduced locomotor activity, and in males, increased aggression. [provided by RefSeq, Nov 2016]

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