Tpr (NM_001107185) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208584 representing NM_001107185 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCCCGGGGTTTTGACGGGTCCCGTCGCGGGAGTCTCCGGCGGGCAGGCGCGCGGGAGACTGCGA GCGAGGCGGCGGACGGGGCGGCTCAGGCGTCTGGGCTCCGCGCATCTCCTTGCTCCTTCGCTTCTCCTTC AGCCGCTGCTGCCACGACCCCGGCCGACATGGCGGCGGTGTTGCAGCAAGTGCTGGAGCGCCCGGAGCTG AACAAGCTGCCTAAGTCGACCCAGAACAAACTTGAGAAGTTCCTGGCTGAACAGCAGTCCGAAATCGACT GCCTGAAGGGGCGGCACGAGAAATTTAAGGTGGAGAGTGAGCAACAATACTTTGAGATAGAGAAGAGACT ATCCCAGAGTCAGGAGAGGCTTGTTAATGAAACCCGGGAGTGTCAGAACTTGAGGCTGGAGCTTGAGAAG CTAAATAACCAAGTAAAAGTATTAACTGAGAAAAACAAAGAACTTGAAACTGCTCAAGACCGCAATCTAG GCATTCAGAGCCAGTTTACAAGAGCAAAGGAAGAGTTAGAAGCTGAAAAAAGAGATTTAATCAGAACCAA TGAGAGGTTATCTCAGGAAGTTGAATATTTAACAGAGGATGTTAAACGTCTAAACGAAAAACTTAAAGAA AGCAATACAACGAAGGGTGAACTTCAGTTAAAGCTGGATGAACTTCAAGCTTCTGATGTCACTGTGAAGT ACCGAGAAAAACGCTTAGAACAAGAAAAGGAATTGCTACACAATCAAAATTCATGGCTAAACACAGAGTT GAAAACCAAAACTGATGAGCTATTGGCTCTAGGAAGAGAAAAGGGAAATGAAATTCTGGAACTTAAGTGT ACTCTTGAAAACAAAAAGGAAGAGGTTTTGAGACTGGAAGAACAGATGAATGGCTTGAAAACATCAAATG AGCATCTTCAGAAACATGTAGAGGATTTGTTGACAAAATTGAAAGAGGCCAAAGAACAGCAGGCCAGTAT GGAAGAGAAATTCCACAATGAGTTAAATGCTCACATAAAGCTCTCTAACCTATACAAGAGCGCTGCTGAC GACTCAGAGGCGAAGAGTAATGAACTAACTCGGGCAGTGGATGAGCTGCACAAACTGCTCAAAGAAGCTG GAGAAGCCAACAAAACAATACAAGATCATCTTCTACAAGTGGAAGAATCTAAAGACCAAATGGAAAAAGA AATGCTTGAGAAAATAGGAAAATTGGAAAAGGAATTAGAGAATGCAAATGACCTGCTTTCCGCTACAAAA CGTAAAGGAGCTATATTATCTGAAGAAGAGCTTGCAGCCATGTCTCCTACCGCAGCAGCCGTCGCTAAAA TTGTAAAGCCCGGCATGAAACTAACTGAGCTATATAATGCTTATGTGGAAACTCAGGATCAGTTGCTGTT GGAAAAACTAGAAAATAAAAGGATTAATAAGTATCTGGATGAGATAGTAAAAGAAGTGGAAGCCAAAGCA CCGATTTTGAAACGCCAGCGTGAAGAGTATGAGCGTGCGCAGAAAGCTGTGGCCAGTCTGTCTGCAAAGC TTGAGCAAGCCATGAAGGAGATTCAGCGATTGCAGGAGGACACTGATAAAGCCAACAAACATTCATCAGT ACTTGAAAGGGATAATCAGAGGATGGAAATACAAATAAAAGATCTTTCACAACAGATAAGAGTGCTCTTG ATGGAACTTGAAGAAGCAAGGGGTAACCATGTAATTCGGGATGAGGAGGTCAGCTCTGCTGACATAAGTA GTTCATCTGAAGTAATATCGCAGCATCTAGTATCTTACAGGAACATTGAAGAGCTTCAGCAGCAGAATCA GCGTCTGCTGTTTGCTCTTCGAGAACTTGGGGAAACCAGGGAACGAGAAGAACAAGAGACAACTTCCTCT AAAATTGCTGAACTTCAGAATAAACTGGAGAACTCTCTCACTGAACTGGAACAGCTCCGTGAGTCACGAC AGCATCAGATGCAGCTTGTTGATTCCATAGTTCGTCAGCGTGACATGTACCGAATTTTGTTGTCACAAAC AACGGGAATGGCCATTCCATTACAAGCTTCAAGTTTAGATGATATTTCTCTTGTGTCAACTCCAAAACGT TCAAGTACATCACAGACTGTTTCTACTCCTGCTCCGGAACCCATCATTGAGTCTACAGAGACCATAGAAG CAAAGGCTGCCCTTAAGCAGTTGCAGGAAATCTTTGAGAACTATAAAAAAGAAAAAATGGACAGTGAGAA ACTGCAGAATGAGCAGCTAGAGAAACTTCAAGAGCAAGTCACAGACCTGCGCTCACAAAACACCAAAATT TCTACTCAGCTAGATTTCGCTTCTAAACGTTACGAAATGCTTCAAGATAATGTAGAAGGATATCGCCGAG AAATAACATCTCTACAAGAGAGAAATCAGAAACTCACTGCAACTACGCAGAAGCAGGAACAGATCATCAA CACGATGACACAAGACTTGAGAGGAGCCAATGAGAAGCTAGCTGTTGCAGAAGTAAGAGCAGAAAATTTG AAGAAGGAAAAAGAAATGCTTAAATTGTCTGAAGTCCGTCTGTCTCAGCAAAGAGAGTCTTTGTTAGCTG AACAGAGGGGACAGAACTTGTTGCTGACTAATCTGCAAACCATCCAGGGCATACTAGAGCGATCTGAGAC TGAAACCAAACAAAGGCTGAGTAGCCAAATAGAAAAATTGGAGCATGAGATCTCTCACCTGAAGAAGAAG TTGGAAAACGAGGTGGAACAAAGACATACACTTACGAGGAATCTAGATGTTCAACTACTAGATACCAAGA GACAGCTAGATACAGAAATAAATCTTCATCTTAACACAAAAGAGCTGTTAAAAAATGCTCAGAAGGATAT TGCTACACTGAAACAGCACCTTAATAATATGGAAGCCCAGCTTGCTTCTCAGTCCACACAGAGAACCGGT AAAGGTCAGCCTGGTGACAGAGATGATGTTGATGATCTTAAAAGTCAGCTAAGGCAGGCTGAAGAACAGG TCAATGACTTGAAGGAGAGACTCAAGACAAGTGCTAGTAACGTAGAGCAGTATAGAGCCATGGTGACCAG CTTAGAAGACTCCCTGAACAAGGAGAAACAGGTGACTGAGGAGGTACATAAGAACATTGAAGTGCGTTTG AAAGAGTCAGCAGAGTTTCAGACACAGTTGGAGAAAAAGCTGATGGAAGTAGAGAAGGAGAAGCAAGAAC TTCAGGATGACAAGAGGAAAGCCATCGAAAGCATGGAACAGCAATTAACTGAATTGAAGAAAACACTGTC TAGTGTTCAGAGTGAAGTACAAGAAGCTCTTCAGAGAGCAAGCACAGCTTTAAGTAATGAACAGCAAGCC AGGCGTGACTGTCAGGAACAAGCTAAAATAGCGGTGGAAGCTCAGAATAAGTATGAGCGAGAATTGATGC TACATGCTGCTGATGTCGAAGCTCTGCAAGCTGCCAAGGAACAGGTTTCAAAAATGGCATCAGTCCGTCA GCATTTGGAAGAAACAACTCAGAAAGCAGAATCACAGTTGTTGGAATGTAAAGCTTCTTGGGAAGAAAGA GAGAGAGTGTTGAAGGATGAAGTTTCCAAGAGTGTGTCTCGCTGTGAAGATCTAGAGAAACAAAACAGAC TACTTCATGATCAGATCGAAAAGTTAAGTGACAAGGTGGTCACCTCTATGAAGGAGGTTGTGCAATCACC ATTAAATATATCTCTCAATGAAGAAGGAAAATCTCAAGAACAAATTTTAGAAATTCTTAGATTTATTCGA CGAGAAAAAGAAATTGCTGAAACTAGGTTTGAGGTGGCTCAGGTTGAAAGTCTGCGATATCGACAAAGAG TTGAACTTCTGGAACGGGAGTTGCAGGAACTGCAGGATAGCCTGAACGCAGAAAGGGAAAAGGTGCAGGT AACTGCAAAAACAATGGCTCAACATGAAGAACTGATGAAGAAAACCGAAACAATGAATGTAGTTATGGAG ACCAATAAGATGCTAAGGGAGGAAAAGGAAAGGCTCGAACAGAACCTACAACAAATGCAAGCCAAGGTGA GGAAACTGGAATTGGACATTTTACCCTTACAAGAAGCCAATGCTGAGCTGAGTGAGAAAAGTGGCATGCT CCAGGCAGAAAAGAAGCTACTAGAAGAGGATGTCAAACGTTGGAAAGCGCGGAACCAGCATCTAATAAAT CAGCAGAAAGATCCAGATACAGAAGAATATCGAAAGTTGCTTTCTGAAAAGGAAATTCACACTAAGAGAA TTCAGCAGCTAAATGAAGAAGTTGGAAGACTTAAGGCTGAAATTGCAAGATCAAATGCATCCTTGACTAA CAACCAGAATTTAATCCAGAGTCTAAAGGAAGATTTAAGTAAAGTAAGAACTGAAAAGGAAAGCATTCAG AAAGATTTAGATGCCAAAATAATTGATATACAGGAAAAAGTGAAAACAATCACTCAAGTCAAGAAGATCG GACGCAGGTACAAGACTCAGTTTGAAGAACTTAAAGCACAGCAGAAGGCCATGGAGACTTCCACTCAGTC TTCAGGAGACCATCAGGAACAGCACATCTCTGTCCAGGAAATGCAAGAGCTCAAAGATAACCTCAGCCAA TCTGAAACAAAGACAAAGTCACTTGAGGGTCAAGTAGAGAATCTGCAGAAGACCTTATCTGAAAAAGAGA CAGAAGCAAGAAGCCTCCAGGAGCAGACAGCGCAGCTTCAGTCTGAGCTGTCCCGCCTCCGCCAGGAACT CCAGGATAAAACCACAAAGGAGGAGCAGCTCCGACAGCAAATGAATGAAAAGGATGAGAAAACATGGAAG GCCATTACAGTGGCCAGATCAAAAATTGCACACCTATCTGGTGTGAAAGATCAGTTAACTAAAGAAAATG AAGAGCTTAAACAAAGGAATGGAGCTTTAGATCAGCAGAAAGATGAACTCGATGTCCGAATGACTGCTCT GAAGTCCCAGTATGAAGGTCGCATCAGTCGCTTAGAGAGAGAGCTCAGAGAGCATCAAGAGAGACACCTT GAGCAGAGAGATGAACCTCAGGAACCCACTAATAAGGCTCCAGAACAGCAGAGACAAATCACACTGAAAA CCACTCCAGCTTCTGGTGAAAGAGGAATTGCCAGTACATCAGACCCACCAACAGCCAATATCAAGCCAAC TCCTGTTGTTTCTACTCCAAGTAAAGTGACAGCTGCAGCTATGGCTGGGAATAAGTCGACACCCAGGGCA AGTATCCGCCCAATGGTTACACCTGCAACTGTCACAAACCCAACCACTACCCCAACAGCAACCGTAATGC CTACTACACAGGTGGAGTCACAGGAAGCTATGCAGTCAGAAGGGCCTGTGGAGCACGTTCCAGTGTTCGG AAGCACAAGTGGATCTGTCCGTTCTACTAGTCCTAACGTCCAGCCCTCCATTTCTCAACCCCTCCTGACT GTACAGCAGCAAACACAGGCTACAGCTTTTGTACAGCCCACTCAGCAGAGTCACCCTCAGATTGAACCTG CAAATCAAGAGTTGTCCCCAAACATAGTTGAGGTGGTACAGAGTTCACCAGTGGAACGACCATCTACCTC CACAGCAGTATTTGGCACTGTTTCAGCTACCCCAAGCTCCTCCTTGCCAAAGCGCGCTCGGGAGGAAGAG GAGGACAGCACAATAGAAGCAGGAGACCAAGTCTCTGATGACACAGTGGAAATGCCTCTCCCAAAGAAGC TGAAGACGGTCACACCTGTAGGAACTGAGGAAGAAGTTATGGCGGAAGAAAGTACTGATGGAGAGGCAGA AACTCAGACCTATAATCAGGATTCTCAAGATTCCATTGGAGAAGGAGTCACCCAGGGAGATTATACACCA ATGGAAGACAGTGAAGAAACATCACAATCTCTACAAATTGATCTTGGGCCACTTCAATCAGATCAGCAGA CAACTTCCTCTCAGGATGGTCAAGGCAAAGGAGATGATGTAATTGTAATTGACAGTGATGATGAAGATGA TGATGAAGAAAATGATGGAGAACATGAAGATTATGAGGAAGATGAGGATGAGGACGACGATGAGGAAGAT GACACGGGGATGGGAGATGAGGGTGAAGACAGCAATGAGGGAACTGGCAGTGCAGATGGTAATGATGGAT ACGAAGCTGATGACGCCGAGGGTGGTGATGGAACTGATCCAGGCACAGAGACAGAAGAAAGTATGGGTGG AGCTGAAAGTAATCAGAGAGCTGCTGATTCTCAGAACAGTGGTGAAGGAAATACAAGTGCAGCAGAGTCT TCTTTCTCTCAGGAGGTTGCCAGAGAACAGCAGCCCACGTCAGCTTCTGAGAGACAGACCCCCCAAGCGC CACAGTCACCAAGGCGCCCACCACATCCTCTTCCCCCACGCCTGACCATCCATGCCCCGCCTCAGGAGTT GGGGCCACCAGTTCAGAGAATTCAGATGACCCGGAGGCAGTCTGTAGGGCGTGGTCTTCAGTTAACCCCA GGAATAGGTGGCATGCAACAACACTTTTTCGATGACGAAGACAGAACAGTTCCGAGTACCCCAACCCTTG TGGTGCCACATCGCACTGATGGGTTTGCTGAAGCTATTCATTCTCCACAGGTTGCCGGTGTTCCTAGATT CCGGTTTGGGCCACCTGAAGACATGCCACAGACAAGTTCCAGTCACTCTGATCTTGGCCAACTTGCTTCT CAAGGAGGTTTGGGAATGTATGAAACACCCCTCTTCCTGGCTCATGAAGAAGAGTCTGGTGGCCGCAGTG TTCCCACTACGCCTCTCCAAGTAGCAGCCCCAGTGACCGTGTTTACTGAGAGTACCACCTCTGATGCTTC AGAACATGCCTCTCAGTCTGTTCCAATGGTGACAACATCTACAGGCACCTTATCCACGACAAATGAAACC CCAGCCGGTGATGACGGAGATGAAGTATTTGTAGAGACGGAGTCTGAAGGTATTAGTTCAGAGGCAGGCC TAGAAATTGACAGCCAGCAGGAAGAGGAGCCTGTCCAGGCATCTGATGAATCAGACCTCCCCTCAACCAG CCAAGACCCCCCTTCCAGCTCCTCCGTAGATACCAGTAGTAGTCAGCCAAAGCCTTTCAGACGAGTAAGA CTTCAGACAACCTTGAGACAGGGTGTTCGTGGTCGTCAGTTTAACCGACAAAGAGGTATAAGCCATGCAA TGGGAGGAAGAGGAGGAATAAATAGAGGGAATATTAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208584 representing NM_001107185 Red=Cloning site Green=Tags(s) MTSRGFDGSRRGSLRRAGARETASEAADGAAQASGLRASPCSFASPSAAAATTPADMAAVLQQVLERPEL NKLPKSTQNKLEKFLAEQQSEIDCLKGRHEKFKVESEQQYFEIEKRLSQSQERLVNETRECQNLRLELEK LNNQVKVLTEKNKELETAQDRNLGIQSQFTRAKEELEAEKRDLIRTNERLSQEVEYLTEDVKRLNEKLKE SNTTKGELQLKLDELQASDVTVKYREKRLEQEKELLHNQNSWLNTELKTKTDELLALGREKGNEILELKC TLENKKEEVLRLEEQMNGLKTSNEHLQKHVEDLLTKLKEAKEQQASMEEKFHNELNAHIKLSNLYKSAAD DSEAKSNELTRAVDELHKLLKEAGEANKTIQDHLLQVEESKDQMEKEMLEKIGKLEKELENANDLLSATK RKGAILSEEELAAMSPTAAAVAKIVKPGMKLTELYNAYVETQDQLLLEKLENKRINKYLDEIVKEVEAKA PILKRQREEYERAQKAVASLSAKLEQAMKEIQRLQEDTDKANKHSSVLERDNQRMEIQIKDLSQQIRVLL MELEEARGNHVIRDEEVSSADISSSSEVISQHLVSYRNIEELQQQNQRLLFALRELGETREREEQETTSS KIAELQNKLENSLTELEQLRESRQHQMQLVDSIVRQRDMYRILLSQTTGMAIPLQASSLDDISLVSTPKR SSTSQTVSTPAPEPIIESTETIEAKAALKQLQEIFENYKKEKMDSEKLQNEQLEKLQEQVTDLRSQNTKI STQLDFASKRYEMLQDNVEGYRREITSLQERNQKLTATTQKQEQIINTMTQDLRGANEKLAVAEVRAENL KKEKEMLKLSEVRLSQQRESLLAEQRGQNLLLTNLQTIQGILERSETETKQRLSSQIEKLEHEISHLKKK LENEVEQRHTLTRNLDVQLLDTKRQLDTEINLHLNTKELLKNAQKDIATLKQHLNNMEAQLASQSTQRTG KGQPGDRDDVDDLKSQLRQAEEQVNDLKERLKTSASNVEQYRAMVTSLEDSLNKEKQVTEEVHKNIEVRL KESAEFQTQLEKKLMEVEKEKQELQDDKRKAIESMEQQLTELKKTLSSVQSEVQEALQRASTALSNEQQA RRDCQEQAKIAVEAQNKYERELMLHAADVEALQAAKEQVSKMASVRQHLEETTQKAESQLLECKASWEER ERVLKDEVSKSVSRCEDLEKQNRLLHDQIEKLSDKVVTSMKEVVQSPLNISLNEEGKSQEQILEILRFIR REKEIAETRFEVAQVESLRYRQRVELLERELQELQDSLNAEREKVQVTAKTMAQHEELMKKTETMNVVME TNKMLREEKERLEQNLQQMQAKVRKLELDILPLQEANAELSEKSGMLQAEKKLLEEDVKRWKARNQHLIN QQKDPDTEEYRKLLSEKEIHTKRIQQLNEEVGRLKAEIARSNASLTNNQNLIQSLKEDLSKVRTEKESIQ KDLDAKIIDIQEKVKTITQVKKIGRRYKTQFEELKAQQKAMETSTQSSGDHQEQHISVQEMQELKDNLSQ SETKTKSLEGQVENLQKTLSEKETEARSLQEQTAQLQSELSRLRQELQDKTTKEEQLRQQMNEKDEKTWK AITVARSKIAHLSGVKDQLTKENEELKQRNGALDQQKDELDVRMTALKSQYEGRISRLERELREHQERHL EQRDEPQEPTNKAPEQQRQITLKTTPASGERGIASTSDPPTANIKPTPVVSTPSKVTAAAMAGNKSTPRA SIRPMVTPATVTNPTTTPTATVMPTTQVESQEAMQSEGPVEHVPVFGSTSGSVRSTSPNVQPSISQPLLT VQQQTQATAFVQPTQQSHPQIEPANQELSPNIVEVVQSSPVERPSTSTAVFGTVSATPSSSLPKRAREEE EDSTIEAGDQVSDDTVEMPLPKKLKTVTPVGTEEEVMAEESTDGEAETQTYNQDSQDSIGEGVTQGDYTP MEDSEETSQSLQIDLGPLQSDQQTTSSQDGQGKGDDVIVIDSDDEDDDEENDGEHEDYEEDEDEDDDEED DTGMGDEGEDSNEGTGSADGNDGYEADDAEGGDGTDPGTETEESMGGAESNQRAADSQNSGEGNTSAAES SFSQEVAREQQPTSASERQTPQAPQSPRRPPHPLPPRLTIHAPPQELGPPVQRIQMTRRQSVGRGLQLTP GIGGMQQHFFDDEDRTVPSTPTLVVPHRTDGFAEAIHSPQVAGVPRFRFGPPEDMPQTSSSHSDLGQLAS QGGLGMYETPLFLAHEEESGGRSVPTTPLQVAAPVTVFTESTTSDASEHASQSVPMVTTSTGTLSTTNET PAGDDGDEVFVETESEGISSEAGLEIDSQQEEEPVQASDESDLPSTSQDPPSSSSVDTSSSQPKPFRRVR LQTTLRQGVRGRQFNRQRGISHAMGGRGGINRGNIN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107185
ORF Size	7248 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107185.1, NP_001100655.2
RefSeq Size	7527 bp
RefSeq ORF	7251 bp
Locus ID	304862
MW	272.8 kDa

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