Abcc6 (NM_031013) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mrp6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208487 representing NM_031013 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGAGAGCACTCAATGGCCACGCCTGGAGAGTCCTGCGCAGGCCTGAGGGTCTGGAACCAGACAG AACAGGAGCCTGTGGCCTATCACTTGCTCAACCTGTGCTTCCTGCGAGCCGCCGGGAGCTGGGTGCCCCC CATGTACCTCTGGGTCCTTGGCCCCATCTACCTCCTCTACATCCATCGCCATGGCTGCTGCTACCTCCGG ATGTCCCGCCTCTTCAAAATCAAAATGGTGCTCGGCTTTGCCCTCATCCTTCTCTACACCTTCAACGCGG CCGTGCCTCTCTGGAGGATCCACCGGGGCATGCCCCAGGCCCCAGAGCTTCTCATTCACCCTACCGTGTG GCTCACCACCATGAGCTTCGCCACCTTCCTGATCCACATGGAGAGAAAGAAGGGGGTCCGTGCATCTGGG TTGTTGTTCGGGTACTGGCTGCTCTGCTGCCTCGTGCCAGCCATCGACACTGTCCAGCAGGCCTCCGCAG GGAGCTTCCGCCAGGAGCCCCTCCACCACCTGGCCACCTACCTGTGCTTGTCCCTGGTGGTGGCAGAGCT GGTGCTGTCTTGTCTGGTAGACCAGCCACCCTTCTTCTCGGAAGACTCCAAGCCATTGAATCCATGTCCA GAGGCCGAGGCCTCTTTTCCCTCCAAGGCCATGTTCTGGTGGGCCTCTGGACTGCTATGGAAGGGCTACA GGAAACTGCTGGGGCCAAAAGACCTCTGGTCACTTGAGAGAGAAAACTCTTCAGAAGAACTTGTTTCCCA GCTGGAAAGAGAATGGAGGAGGAACTTCAGTGAGCTGCCGGGGCACAAAGGGCACAGTGGTATGGGGACC CCCGAGACAGAGGCCTTCCTGCAGCCAGAGAGGAGCCAGCGGGGCCCGCTGCTCAGGGCTATCTGGCGTG TGTTCCGGTCCACTTTCCTGCTGGGGACCCTCAGCCTGGTCATTAGCGATGCCTTCAGGTTTGCTGTTCC CAAGCTCCTCAGTCTGTTTCTGGAGTTCATGGGCGACCTCGAGTCCTCGGCTTGGACGGGCTGGCTCCTG GCTGTGCTGATGTTCTTGTCGGCCTGCCTACAGACACTGTTTGAACAGCAGTACATGTACAGAGTCAAGG TCCTGCAGATGAGGCTGCGAACAGCCATCACTGGCCTGGTGTACAGAAAGGTCCTGGTCCTGTCCAGTGG TTCCAGAAAGTCCAGTGCAGCAGGGGACGTGGTCAACCTGGTGTCAGTGGACGTACAGCGGCTGGTCGAG AGCATCCTCCACCTCAACGGGCTGTGGCTGCTCTTCCTGTGGATCATTGTGTGCTTTGTCTACCTGTGGC AGCTCCTTGGGCCCTCTGCCCTCACAGCCGTTGCTGTCTTCCTGAGCCTTCTCCCCCTGAACTTCTTCAT TACCAAGAAGAGGAGCTTCCATCAGGAAGAACAGATGAGGCAGAAGGCCTCCCGAGCACGGCTCACCAGC TCCATGCTCAGAACTGTGAGAACCATCAAGTCCCACGGCTGGGAGTGTGCCTTCCTGGAGCGACTCCTGC ATATCCGGGGCCAGGAGCTAGGTGCCCTGAAGACCTCCGCCTTCCTCTTCTCTGTGTCTCTCGTGTCCTT CCAAGTGTCTACATTTCTGGTGGCGCTGGTTGTGTTTGCTGTCCACACCCTGGTGGCAGAGGACAACGCC ATGGATGCGGAGAAGGCGTTTGTGACGCTCACGGTGCTCAGCATCCTTAACAAAGCCCAGGCCTTCCTCC CCTTCTCTGTGCACTGCCTCGTTCAGGCTCGGGTGTCCTTTGACCGCCTAGCTGCTTTCCTGTGCCTGGA AGAAGTAGACCCCAATGGCATGGTCTTGAGTCCCTCCAGATGCTCCTCGAAGGATCGAATTTCTATACAC AATGGCACCTTCGCTTGGTCCCAGGAGAGCCCGCCCTGCCTGCACGGGATCAACCTCACCGTGCCCCAGG GCTGTCTGCTGGCTGTTGTGGGTCCAGTGGGGGCTGGAAAGTCCTCCCTGCTGTCTGCCCTGCTTGGGGA GCTGTTGAAGGTAGAAGGGTCTGTGAGCATTGAGGGTTCCGTGGCCTACGTGCCTCAGGAGGCTTGGGTC CAGAATACCTCTGTGGTGGAGAATGTGTGCTTCAGGCAGGAGCTGGATCTGCCATGGTTGCAGGAAGTTC TAGAAGCCTGTGCCTTGGGGTCTGATGTGGCCAGCTTCCCTGCAGGAGTTCACACCCCAGTAGGGGAGCA GGGCATGAATCTTTCTGGGGGCCAGAAGCAGCGGCTGAGCTTGGCTCGGGCTGTGTACAGAAGGGCTGCT GTGTACCTGATGGATGACCCCCTAGCAGCCCTGGATGCGCATGTCAGCCAGGAAGTCTTCAAACAGGTCA TTGGCCCCAGTGGACTTCTCCAAGGTACGACTCGGATCCTTGTAACACACACGCTGCATGTCCTGCCCCA AGCTGACCAGATCCTGGTGCTGGCCAATGGGACCATCGCAGAGATGGGCTCCTACCAAGACCTTCTGCAT AGGAACGGAGCCCTGGTGGGTCTTCTGGATGGAGCCAGACAGCCTGCAGGCGAAGGAGAAGGAGAAGCAC ATGCTGCAGCCACCAGTGATGACCTTGGAGGCTTTTCTGGAGGTGGGACGCCCACGCGCAGACCAGAGAG GCCCAGACCCAGTGACGCAGCCCCTGTGAAGGGCAGTACTTCAGAGGCACAGATGGAGCCTTCTCTGGAT GACGTTGAGGTCACTGGACTGACAGCAGGAGAGGACAGTGTGCAGTATGGCCGGGTGAAGAGCGCCACAT ACCTGAGCTACCTGCGGGCGGTGGGCACACCGCTCTGCACCTACACCCTGTTCCTCTTCCTCTGCCAGCA AGTGGCGTCCTTCTGCCAAGGCTACTGGCTGAGCCTCTGGGCCGACGACCCGGTCGTGGATGGGAAGCAG ATGCATTCAGCCCTGCGTGGCTCCATCTTTGGACTCCTTGGCTGTCTGCAAGCCATCGGACTGTTTGCCT CCATGGCTGCGGTGTTCCTGGGTGGAGCCCGAGCTTCATGCCTGCTTTTCCGGAGCCTCCTCTGGGACGT GGCTCGCTCTCCCATTGGCTTCTTTGAGCGCACACCAGTCGGGAACCTGCTGAACCGTTTTTCCAAGGAG ACGGACATAGTGGATGTGGACATCCCAGACAAGATGAGGACCCTGCTGACCTATGCCTTTGGACTCCTGG AGGTTGGCCTGGCAGTGTCGATGGCCACACCACTGGCTATTGTGGCCATCCTACCTCTTATGCTCCTTTA TGCTGGGTTTCAGAGCCTCTACGTGGCCACATGTTGCCAGCTGAGACGCCTGGAGTCGGCCAGTTACTCC TCAGTGTGTTCCCATCTGGCTGAGACCTTCCAGGGCAGTCAGGTGGTCAGGGCCTTCCAGGCCCAGGGGC CCTTCACAGCTCAGCACGATGCCCTCATGGATGAGAACCAGAGGATCAGTTTCCCGAGGCTGGTGGCTGA CAGGTGGCTGGCTGCCAACCTGGAGCTCCTGGGGAATGGCCTGGTGTTTGTGGCCGCTACATGTGCTGTG CTGAGCAAGGCTCACCTGAGTGCTGGCCTCGCGGGCTTCTCGGTTTCTGCTGCCCTCCAGGTAACACAGA CTCTGCAGTGGGTGGTCCGCAGCTGGACAGATCTGGAGAACAGCATGGTGGCCGTGGAGCGAGTACAGGA CTACGTTCACACCCCCAAGGAGGCTCCCTGGAGGCTGCCCTCCTCTGCAGCCCAGCCTCTCTGGCCCTGT GGGGGACAGATTGAGTTCCGAGACTTTGGGCTCAGACACCGACCAGAGCTGCCCATGGCTGTGCAGGGTG TGTCCCTGAAGATCCATGCAGGGGAGAAGGTGGGCATCGTGGGCAGGACAGGGGCCGGGAAGTCCTCCCT GACTTGGGGCCTGCTGCGGCTTCAGGAGGCCACTGAGGGTGGTATTTGGATCGATGGGGTCCCCATCACC GACATGGGGCTGCACACACTGCGGTCCAGAATCACCATCATCCCTCAGGACCCTGTCCTGTTCCCGGGCT CGCTGCGGATGAACCTGGACCTGCTTCAGGAGAACACAGATGAGGGCATCTGGGCAGCGCTGGAGACGGT GCAGCTCAAGGCCTTCGTGACCAGCCTGCCTGGCCAGCTGCAGTATGAGTGCTCAGGCCAGGGAGATGAC CTGAGTGTGGGTCAGAAGCAGCTCCTGTGTCTGGCACGTGCCCTTCTCCGGAAAACCCAGATCCTCATCC TGGATGAAGCCACTGCCTCCGTGGACCCAGGGACGGAGATACAGATGCAGGCGGCCCTCGAGCGCTGGTT TGCACAGTGTACAGTGCTGCTCATTGCTCACCGCCTGCGCTCCGTGATGAACTGCGCCAGGGTTCTAGTC ATGGATGAGGGGCAGGTGGCAGAGAGTGGCAGTCCAGCACAGCTGCTGGCCCAGAAAGGCCTGTTTTACA GGCTAGCCCAGGAGTCGGGCCTAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208487 representing NM_031013 Red=Cloning site Green=Tags(s) MNGEHSMATPGESCAGLRVWNQTEQEPVAYHLLNLCFLRAAGSWVPPMYLWVLGPIYLLYIHRHGCCYLR MSRLFKIKMVLGFALILLYTFNAAVPLWRIHRGMPQAPELLIHPTVWLTTMSFATFLIHMERKKGVRASG LLFGYWLLCCLVPAIDTVQQASAGSFRQEPLHHLATYLCLSLVVAELVLSCLVDQPPFFSEDSKPLNPCP EAEASFPSKAMFWWASGLLWKGYRKLLGPKDLWSLERENSSEELVSQLEREWRRNFSELPGHKGHSGMGT PETEAFLQPERSQRGPLLRAIWRVFRSTFLLGTLSLVISDAFRFAVPKLLSLFLEFMGDLESSAWTGWLL AVLMFLSACLQTLFEQQYMYRVKVLQMRLRTAITGLVYRKVLVLSSGSRKSSAAGDVVNLVSVDVQRLVE SILHLNGLWLLFLWIIVCFVYLWQLLGPSALTAVAVFLSLLPLNFFITKKRSFHQEEQMRQKASRARLTS SMLRTVRTIKSHGWECAFLERLLHIRGQELGALKTSAFLFSVSLVSFQVSTFLVALVVFAVHTLVAEDNA MDAEKAFVTLTVLSILNKAQAFLPFSVHCLVQARVSFDRLAAFLCLEEVDPNGMVLSPSRCSSKDRISIH NGTFAWSQESPPCLHGINLTVPQGCLLAVVGPVGAGKSSLLSALLGELLKVEGSVSIEGSVAYVPQEAWV QNTSVVENVCFRQELDLPWLQEVLEACALGSDVASFPAGVHTPVGEQGMNLSGGQKQRLSLARAVYRRAA VYLMDDPLAALDAHVSQEVFKQVIGPSGLLQGTTRILVTHTLHVLPQADQILVLANGTIAEMGSYQDLLH RNGALVGLLDGARQPAGEGEGEAHAAATSDDLGGFSGGGTPTRRPERPRPSDAAPVKGSTSEAQMEPSLD DVEVTGLTAGEDSVQYGRVKSATYLSYLRAVGTPLCTYTLFLFLCQQVASFCQGYWLSLWADDPVVDGKQ MHSALRGSIFGLLGCLQAIGLFASMAAVFLGGARASCLLFRSLLWDVARSPIGFFERTPVGNLLNRFSKE TDIVDVDIPDKMRTLLTYAFGLLEVGLAVSMATPLAIVAILPLMLLYAGFQSLYVATCCQLRRLESASYS SVCSHLAETFQGSQVVRAFQAQGPFTAQHDALMDENQRISFPRLVADRWLAANLELLGNGLVFVAATCAV LSKAHLSAGLAGFSVSAALQVTQTLQWVVRSWTDLENSMVAVERVQDYVHTPKEAPWRLPSSAAQPLWPC GGQIEFRDFGLRHRPELPMAVQGVSLKIHAGEKVGIVGRTGAGKSSLTWGLLRLQEATEGGIWIDGVPIT DMGLHTLRSRITIIPQDPVLFPGSLRMNLDLLQENTDEGIWAALETVQLKAFVTSLPGQLQYECSGQGDD LSVGQKQLLCLARALLRKTQILILDEATASVDPGTEIQMQAALERWFAQCTVLLIAHRLRSVMNCARVLV MDEGQVAESGSPAQLLAQKGLFYRLAQESGLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031013
ORF Size	4506 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031013.1, NP_112275.1
RefSeq Size	5775 bp
RefSeq ORF	4509 bp
Locus ID	81642
UniProt ID	O88269
MW	165 kDa
Gene Summary	human homolog acts as a Mg-ATP-dependent efflux pump that transports glutathione S-conjugates and mediates a low level of resistance to some anticancer agents [RGD, Feb 2006]

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