Abcc6 (NM_031013) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Mrp6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208487 representing NM_031013 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGAGAGCACTCAATGGCCACGCCTGGAGAGTCCTGCGCAGGCCTGAGGGTCTGGAACCAGACAG AACAGGAGCCTGTGGCCTATCACTTGCTCAACCTGTGCTTCCTGCGAGCCGCCGGGAGCTGGGTGCCCCC CATGTACCTCTGGGTCCTTGGCCCCATCTACCTCCTCTACATCCATCGCCATGGCTGCTGCTACCTCCGG ATGTCCCGCCTCTTCAAAATCAAAATGGTGCTCGGCTTTGCCCTCATCCTTCTCTACACCTTCAACGCGG CCGTGCCTCTCTGGAGGATCCACCGGGGCATGCCCCAGGCCCCAGAGCTTCTCATTCACCCTACCGTGTG GCTCACCACCATGAGCTTCGCCACCTTCCTGATCCACATGGAGAGAAAGAAGGGGGTCCGTGCATCTGGG TTGTTGTTCGGGTACTGGCTGCTCTGCTGCCTCGTGCCAGCCATCGACACTGTCCAGCAGGCCTCCGCAG GGAGCTTCCGCCAGGAGCCCCTCCACCACCTGGCCACCTACCTGTGCTTGTCCCTGGTGGTGGCAGAGCT GGTGCTGTCTTGTCTGGTAGACCAGCCACCCTTCTTCTCGGAAGACTCCAAGCCATTGAATCCATGTCCA GAGGCCGAGGCCTCTTTTCCCTCCAAGGCCATGTTCTGGTGGGCCTCTGGACTGCTATGGAAGGGCTACA GGAAACTGCTGGGGCCAAAAGACCTCTGGTCACTTGAGAGAGAAAACTCTTCAGAAGAACTTGTTTCCCA GCTGGAAAGAGAATGGAGGAGGAACTTCAGTGAGCTGCCGGGGCACAAAGGGCACAGTGGTATGGGGACC CCCGAGACAGAGGCCTTCCTGCAGCCAGAGAGGAGCCAGCGGGGCCCGCTGCTCAGGGCTATCTGGCGTG TGTTCCGGTCCACTTTCCTGCTGGGGACCCTCAGCCTGGTCATTAGCGATGCCTTCAGGTTTGCTGTTCC CAAGCTCCTCAGTCTGTTTCTGGAGTTCATGGGCGACCTCGAGTCCTCGGCTTGGACGGGCTGGCTCCTG GCTGTGCTGATGTTCTTGTCGGCCTGCCTACAGACACTGTTTGAACAGCAGTACATGTACAGAGTCAAGG TCCTGCAGATGAGGCTGCGAACAGCCATCACTGGCCTGGTGTACAGAAAGGTCCTGGTCCTGTCCAGTGG TTCCAGAAAGTCCAGTGCAGCAGGGGACGTGGTCAACCTGGTGTCAGTGGACGTACAGCGGCTGGTCGAG AGCATCCTCCACCTCAACGGGCTGTGGCTGCTCTTCCTGTGGATCATTGTGTGCTTTGTCTACCTGTGGC AGCTCCTTGGGCCCTCTGCCCTCACAGCCGTTGCTGTCTTCCTGAGCCTTCTCCCCCTGAACTTCTTCAT TACCAAGAAGAGGAGCTTCCATCAGGAAGAACAGATGAGGCAGAAGGCCTCCCGAGCACGGCTCACCAGC TCCATGCTCAGAACTGTGAGAACCATCAAGTCCCACGGCTGGGAGTGTGCCTTCCTGGAGCGACTCCTGC ATATCCGGGGCCAGGAGCTAGGTGCCCTGAAGACCTCCGCCTTCCTCTTCTCTGTGTCTCTCGTGTCCTT CCAAGTGTCTACATTTCTGGTGGCGCTGGTTGTGTTTGCTGTCCACACCCTGGTGGCAGAGGACAACGCC ATGGATGCGGAGAAGGCGTTTGTGACGCTCACGGTGCTCAGCATCCTTAACAAAGCCCAGGCCTTCCTCC CCTTCTCTGTGCACTGCCTCGTTCAGGCTCGGGTGTCCTTTGACCGCCTAGCTGCTTTCCTGTGCCTGGA AGAAGTAGACCCCAATGGCATGGTCTTGAGTCCCTCCAGATGCTCCTCGAAGGATCGAATTTCTATACAC AATGGCACCTTCGCTTGGTCCCAGGAGAGCCCGCCCTGCCTGCACGGGATCAACCTCACCGTGCCCCAGG GCTGTCTGCTGGCTGTTGTGGGTCCAGTGGGGGCTGGAAAGTCCTCCCTGCTGTCTGCCCTGCTTGGGGA GCTGTTGAAGGTAGAAGGGTCTGTGAGCATTGAGGGTTCCGTGGCCTACGTGCCTCAGGAGGCTTGGGTC CAGAATACCTCTGTGGTGGAGAATGTGTGCTTCAGGCAGGAGCTGGATCTGCCATGGTTGCAGGAAGTTC TAGAAGCCTGTGCCTTGGGGTCTGATGTGGCCAGCTTCCCTGCAGGAGTTCACACCCCAGTAGGGGAGCA GGGCATGAATCTTTCTGGGGGCCAGAAGCAGCGGCTGAGCTTGGCTCGGGCTGTGTACAGAAGGGCTGCT GTGTACCTGATGGATGACCCCCTAGCAGCCCTGGATGCGCATGTCAGCCAGGAAGTCTTCAAACAGGTCA TTGGCCCCAGTGGACTTCTCCAAGGTACGACTCGGATCCTTGTAACACACACGCTGCATGTCCTGCCCCA AGCTGACCAGATCCTGGTGCTGGCCAATGGGACCATCGCAGAGATGGGCTCCTACCAAGACCTTCTGCAT AGGAACGGAGCCCTGGTGGGTCTTCTGGATGGAGCCAGACAGCCTGCAGGCGAAGGAGAAGGAGAAGCAC ATGCTGCAGCCACCAGTGATGACCTTGGAGGCTTTTCTGGAGGTGGGACGCCCACGCGCAGACCAGAGAG GCCCAGACCCAGTGACGCAGCCCCTGTGAAGGGCAGTACTTCAGAGGCACAGATGGAGCCTTCTCTGGAT GACGTTGAGGTCACTGGACTGACAGCAGGAGAGGACAGTGTGCAGTATGGCCGGGTGAAGAGCGCCACAT ACCTGAGCTACCTGCGGGCGGTGGGCACACCGCTCTGCACCTACACCCTGTTCCTCTTCCTCTGCCAGCA AGTGGCGTCCTTCTGCCAAGGCTACTGGCTGAGCCTCTGGGCCGACGACCCGGTCGTGGATGGGAAGCAG ATGCATTCAGCCCTGCGTGGCTCCATCTTTGGACTCCTTGGCTGTCTGCAAGCCATCGGACTGTTTGCCT CCATGGCTGCGGTGTTCCTGGGTGGAGCCCGAGCTTCATGCCTGCTTTTCCGGAGCCTCCTCTGGGACGT GGCTCGCTCTCCCATTGGCTTCTTTGAGCGCACACCAGTCGGGAACCTGCTGAACCGTTTTTCCAAGGAG ACGGACATAGTGGATGTGGACATCCCAGACAAGATGAGGACCCTGCTGACCTATGCCTTTGGACTCCTGG AGGTTGGCCTGGCAGTGTCGATGGCCACACCACTGGCTATTGTGGCCATCCTACCTCTTATGCTCCTTTA TGCTGGGTTTCAGAGCCTCTACGTGGCCACATGTTGCCAGCTGAGACGCCTGGAGTCGGCCAGTTACTCC TCAGTGTGTTCCCATCTGGCTGAGACCTTCCAGGGCAGTCAGGTGGTCAGGGCCTTCCAGGCCCAGGGGC CCTTCACAGCTCAGCACGATGCCCTCATGGATGAGAACCAGAGGATCAGTTTCCCGAGGCTGGTGGCTGA CAGGTGGCTGGCTGCCAACCTGGAGCTCCTGGGGAATGGCCTGGTGTTTGTGGCCGCTACATGTGCTGTG CTGAGCAAGGCTCACCTGAGTGCTGGCCTCGCGGGCTTCTCGGTTTCTGCTGCCCTCCAGGTAACACAGA CTCTGCAGTGGGTGGTCCGCAGCTGGACAGATCTGGAGAACAGCATGGTGGCCGTGGAGCGAGTACAGGA CTACGTTCACACCCCCAAGGAGGCTCCCTGGAGGCTGCCCTCCTCTGCAGCCCAGCCTCTCTGGCCCTGT GGGGGACAGATTGAGTTCCGAGACTTTGGGCTCAGACACCGACCAGAGCTGCCCATGGCTGTGCAGGGTG TGTCCCTGAAGATCCATGCAGGGGAGAAGGTGGGCATCGTGGGCAGGACAGGGGCCGGGAAGTCCTCCCT GACTTGGGGCCTGCTGCGGCTTCAGGAGGCCACTGAGGGTGGTATTTGGATCGATGGGGTCCCCATCACC GACATGGGGCTGCACACACTGCGGTCCAGAATCACCATCATCCCTCAGGACCCTGTCCTGTTCCCGGGCT CGCTGCGGATGAACCTGGACCTGCTTCAGGAGAACACAGATGAGGGCATCTGGGCAGCGCTGGAGACGGT GCAGCTCAAGGCCTTCGTGACCAGCCTGCCTGGCCAGCTGCAGTATGAGTGCTCAGGCCAGGGAGATGAC CTGAGTGTGGGTCAGAAGCAGCTCCTGTGTCTGGCACGTGCCCTTCTCCGGAAAACCCAGATCCTCATCC TGGATGAAGCCACTGCCTCCGTGGACCCAGGGACGGAGATACAGATGCAGGCGGCCCTCGAGCGCTGGTT TGCACAGTGTACAGTGCTGCTCATTGCTCACCGCCTGCGCTCCGTGATGAACTGCGCCAGGGTTCTAGTC ATGGATGAGGGGCAGGTGGCAGAGAGTGGCAGTCCAGCACAGCTGCTGGCCCAGAAAGGCCTGTTTTACA GGCTAGCCCAGGAGTCGGGCCTAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031013
Insert Size	4509 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031013.1, NP_112275.1
RefSeq Size	5775 bp
RefSeq ORF	4509 bp
Locus ID	81642
UniProt ID	O88269
Gene Summary	human homolog acts as a Mg-ATP-dependent efflux pump that transports glutathione S-conjugates and mediates a low level of resistance to some anticancer agents [RGD, Feb 2006]

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