Cilp2 (NM_001107307) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208414 representing NM_001107307 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCACCTCTGCCCTTGCTCTATCTCTGCATCGCTGCCGCACACCTAGCGGGAGCGCGAGATGCCA CCCCCACCGAGGAGCACACTGCAACCGTCAGGGGTCTTCAGGGACGGCCACCAGACCCCGGCCAGCCCTC GCCGGCCCTGGAGGACTGGGAGGAGGCCAGCGAGTGGACCTCGTGGTTCAACGTGGACCACCCGGGAGGA GATGGTGACTTCGAGAGCCTGGCAGCCATTCGCTTCTACTATGGTCCCGCGCGCGTGTGCCCGAGGCCGC TGGCACTGGAGGCGCGCACCACGGACTGGGCACTACCCGCTGCAATAGGCGAGAGGGTGCACGCCAACCC AGAGCGCGGATTCTGGTGTCTTAATCGCGAGCAGCCGCGAGGCCGCCGCTGCTCCAACTACCACGTGCGC TTCCGCTGCCCACTGGATCCACCTTATCTCCCTCCTTGCTCTGCAGAGGCGGCATGGGGCGCTTGGGGCG CTTGGGGCCTCTGTTCCAAGAGTTGCGGGCCAGGCCGTCGCCTGCGCCGTCGCAGCTGTCAAAGCTCATC TGGGGACACATGTCCTGGGAGCCCCCAGGAGGCACAGAAGTGCGTGAGATCCCGTTGCCCAGCTCCCCCC AAGAGTTCTCTCTGCCCCTGCTGCTCCCATGTTCCCCTAGGATGTAGCTCTGACACCTGCGGGTGCCCCA ACCACATCCTCTTGGGCTCGGTGGTCACCCCATCTGGGCGACCGCTGTCAGGAGCCAGGGTCTCCCTGCG AACACGACCTGGCACCGTAGCCACCAGTGACACCCATGGAACCTTCCGGGTGCCTGGAGTCTGCGCTGGC AGCAAGGCCAACGTCAGTGCCCGGATGAATGGCTTCTCTACTGGTACAGCCCAGGCCCATGCCAACACCT CCAACTCTGCTGTGGTCACCATCGTCCTTGAAGAGTTAGGGAAGCCGTACCTGGTAAAGCACCCTGAGTC TCGAGTTCGAGAGGCAGGTCAGAATGTGACCTTCTGCTGCAAGGCCTCTGGGACACCCATGCCCAAGAAA TATTCCTGGTTCCACAACGGGACCTTACTGGACCGACGCCAGCAGGGGTCTGGGCCACACCTAGAGCTCC AGGGGCTTCGTGAGGAGCAGGCAGGGGAGTACCACTGCAAAGCCTGGAACGAGGCGGGCACCGTGCGATC CCGCGCTGCCCACCTCACGGTGCTCGCTCCCGGCCAGCCAGCCTGTGACCCCCGACCTCAGGAACACCTG ATCAAGCTTCCGGATGACTGCGGCCAGCCGGGTGGCGGCCCCACGTACCTAGACGTGGGACTGTGCGCAG ACACGCGCTGCCCGGGCCCCGTGGGCTCCGGCCCGCGCTGTGGGGACGCGGGCTCTCGCTGCTGCTCAGT GCTGCGCCTGGAGAGCAGAGATATCCGCTGCTCTGGCTATGTGCTCCCAGTGAAGGTGGTGGCTGAGTGT GGCTGTCGCAAGTGCCTGCCCCGTAGGGGATTGGTGCGAGGCCGCGTGGTGGCTGCGGACTCTGGGGAGC CCCTGCGTTTCGCCAGGATTCTATTGGGCCTTGCACCCATCGGCTTCACCTCCTACCAGGGCGACTTCAC CATCGAGGTGCCGCCGGCCACCGAGCGCCTCGTGGTGACCTTCGTGGACTCGAGTGGTGAGTTCGTGGAC AGCATCCGCGTGTTGCCTTTCGACCCTCGCGGCGCTGGTGTGTACCACGAGATCCGCGTGCTGCGCAAGG CGGCCGCAGTGCTGCTGGACGCGGAGCAGGGCGGTGAAATTCCGCTGGGCGGCACAGAGGAGGCGCCAGC GCTAGGCGAGCTAGTCTTCCCGCCCCGGACCTTCCACCACCCCGACGGCCGTCCCTACTCGGGGCCCGTG GAGGCGCGTGTCACCTTCGTGGACCCTCGTGACCTGGCGTCGGCGGCCGCGGCGTCCAGCGACCTGCGCT ACCTGGATAGCTCGGGTGAGCTGGCGCCGCTGCGCACCTATGGCATGTTTGCAGTGGACCTGCACGCGCC AGGCTCCACCGAGCAGCTGCACGTGGCGCGCGCGGACGTGCGCGTGGACGCCGACCACGTGCGCATGCCC GGACACGCCGATGCTCTGGCACTCTGGTCGTTGGACCCAGAGACCGGCCTGTGGGAGGAGGAGGGGGCTG AGCAGGGAGGAGGCGGCTTCCGGCGTGAGAAGGAGGCGGTGCGCGTGCGCAGGGAGGAGCGCACGTTCCT CGTGGGCGCGCTCACGGTGCGCGAGCGCCGCTTATTCAACCTAGACGTGCCCGAGCGGCGCCGCTGCTTC GTGAAGGTTCGCGCCTACGGTACAGATCGCTTCGCGCCCTCAGAGCAGGTGCAGGGCGTGGTGGTGACGC TGCTCAATCTGGAACCCGCGCCCGGTTTCACGGCCAACCCACGCGCGTGGGGCCGCTTCGACAGCGCGGT CACAGGGCCCAACGGCGCGTGCGTGCCGGCCTTCTGCGACGCCGAGCGGCCCGACGCCTACACGGCCTTC GTGACCGCGTCTCTAGGAGGCGAGGAGCTGGAGGCCGCGCCGTCGCGGCCACGCGCGACCGCGGCCACCG TGGGCGTGGCAGAGCCCTACCTGGAGCGCCTCGGCTACCAGCGCACCGATCACGACGACCCCGCGCTCAA GCGCACCGGCTTCCGCTTGAACCTTGCACGTCCACGCACGGGCCACGAGTCCGAGGCGCACGGGCCCGTG TATCCGTGGCGCCGCCTGCGCGACTGCGAGGACGCGCCGGTCACCGACAGTCACTTCCGATTCTCGCGCG TAGAGGCAGACAAGTACGAGTACGACGTGGTGCCATTCCACGAGGGAGCGCCCGCCTCGTGGACAGGCGA CCTGCTGGCCTGGTGGCCCAACCCGCAGGAGTTCCGAGCGTGCTTCCTGAAGGTGCGGCTGCAGGGCCCC CAGGAGTACATGGTGCGTTCGCACAACGCGGGCGGCACCCACGAAGACACGCGCGGCCACCTCTATGGGC TGCGCGACACTCGCAGCGTGCGCAACCCTGAGCGCCCGGGCGCCTCGGCCGCCTGCGTGGAGTTTAAGTG CGGTGGCATGCTGTTTGACCAGCGCCAAGTGGACAGGACACTGGTGACTGTGACCCCGCAGGGCAGCTGC CGCCGCGTGGCCGTCAACACGCTCCTGCAGGACTATCTAGCCCGGCACCCGCCGCTCGCCCCCGCGGACG ACCCCGCCACCTTCGCCATGCTGGCTCCGCTTGACGCCCTGGGCCACAACTATGGTGTCTACACCGTCAC CGACCAGAGCCCACGGCTGGCCAAGGAAATCGCCATCGGCCGCTGCTTCGACGGCTCCTCGGACGGCTTC TCCCGGGAGATGAAGGCGGACGCGGGCACAGCGGTTACCTTCCAGTGCCGAGAGCCGCCCGCCAGACCCA GCCTCTTCCAGAGGCTGCTGGAGAACCCCGCATCGGCGCTTGGCGACATTCGTAGGGAGATGGGCCAAGC CGCCCGCGATTCCCGGGTTACCCAAACCCAGGCTGGGGACACCGGCCCCTTCGGTTCTGGACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208414 representing NM_001107307 Red=Cloning site Green=Tags(s) MSSPLPLLYLCIAAAHLAGARDATPTEEHTATVRGLQGRPPDPGQPSPALEDWEEASEWTSWFNVDHPGG DGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPAAIGERVHANPERGFWCLNREQPRGRRCSNYHVR FRCPLDPPYLPPCSAEAAWGAWGAWGLCSKSCGPGRRLRRRSCQSSSGDTCPGSPQEAQKCVRSRCPAPP KSSLCPCCSHVPLGCSSDTCGCPNHILLGSVVTPSGRPLSGARVSLRTRPGTVATSDTHGTFRVPGVCAG SKANVSARMNGFSTGTAQAHANTSNSAVVTIVLEELGKPYLVKHPESRVREAGQNVTFCCKASGTPMPKK YSWFHNGTLLDRRQQGSGPHLELQGLREEQAGEYHCKAWNEAGTVRSRAAHLTVLAPGQPACDPRPQEHL IKLPDDCGQPGGGPTYLDVGLCADTRCPGPVGSGPRCGDAGSRCCSVLRLESRDIRCSGYVLPVKVVAEC GCRKCLPRRGLVRGRVVAADSGEPLRFARILLGLAPIGFTSYQGDFTIEVPPATERLVVTFVDSSGEFVD SIRVLPFDPRGAGVYHEIRVLRKAAAVLLDAEQGGEIPLGGTEEAPALGELVFPPRTFHHPDGRPYSGPV EARVTFVDPRDLASAAAASSDLRYLDSSGELAPLRTYGMFAVDLHAPGSTEQLHVARADVRVDADHVRMP GHADALALWSLDPETGLWEEEGAEQGGGGFRREKEAVRVRREERTFLVGALTVRERRLFNLDVPERRRCF VKVRAYGTDRFAPSEQVQGVVVTLLNLEPAPGFTANPRAWGRFDSAVTGPNGACVPAFCDAERPDAYTAF VTASLGGEELEAAPSRPRATAATVGVAEPYLERLGYQRTDHDDPALKRTGFRLNLARPRTGHESEAHGPV YPWRRLRDCEDAPVTDSHFRFSRVEADKYEYDVVPFHEGAPASWTGDLLAWWPNPQEFRACFLKVRLQGP QEYMVRSHNAGGTHEDTRGHLYGLRDTRSVRNPERPGASAACVEFKCGGMLFDQRQVDRTLVTVTPQGSC RRVAVNTLLQDYLARHPPLAPADDPATFAMLAPLDALGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGF SREMKADAGTAVTFQCREPPARPSLFQRLLENPASALGDIRREMGQAARDSRVTQTQAGDTGPFGSGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107307
ORF Size	3564 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107307.1, NP_001100777.1
RefSeq Size	4081 bp
RefSeq ORF	3567 bp
Locus ID	306356
MW	128.9 kDa

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