Map3k1 (NM_053887) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mekk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208228 representing NM_053887 Red=Cloning site Blue=ORF Green=Tags(s) GCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTC AAGCTTAACTAGCTAGCGGACCGAC ATGGCGGCGGCGGCGGGCGATCGCGCCTCGTCGTCGGGATTCCCGGGCGCCGCGGCGGCGAGCCCCGAGG CGGGCGGCGGCGGCGGAGCTCTCCAGGGAAGCGGCGCGCCCGCAGCGGGCGCGGGGCTGCTGCGGGAGAC TGGCAGCGCGGGCCGCGAGCGCGCGGACTGGCGGCGGCAGCAGCTGCGCAAAGTAAGGAGTGTGGAGCTG GACCAGCTGCCGGAGCAGCCGCTCTTCCTCACCGCCTCGCCGCCCTGCCCATCTACTTCCCCGTCGCCGG AGCCCGCGGACGCGGCTGCAGGAGCGAGTGGCTTCCAGCCTGCGGCGGGACCGCCACCCCCGGGAGCAGC GAGTCGCTGCGGCTCCCACTCTGCCGAGCTGGCGGCCGCGCGGGACAGCGGCGCCCGGAGCCCCGCGGGG GCCGAGCCGCCCTCTGCAGCGGCCCCCTCCGGTCGAGAGATGGAGAATAAAGAAACCCTCAAAGGATTGC ACAAGATGGATGACCGCCCAGAGGAGCGAATGATCAGGGAGAAGCTCAAGGCGACCTGTATGCCTGCCTG GAAGCACGAGTGGTTAGAAAGGAGGAACAGGAGAGGCCCTGTGGTGGTGAAACCAATCCCTATTAAAGGA GATGGATCTGAAATGAGTAACTTGGCAGCTGAGCTCCAAGGAGAGGGCCAGGCAGGCTCTGCTGCACCAG CTCCCAAGGGCCGACGAAGCCCGTCTCCTGGAAGCTCCCCATCAGGGCGCTCAGGGAAGCCGGAATCCCC AGGAGTGAGACGGAAACGAGTGTCCCCAGTGCCTTTCCAGAGTGGCAGAATCACACCACCTCGAAGAGCC CCGTCGCCAGATGGCTTCTCACCGTACAGCCCAGAGGAGACGAGCCGCCGTGTGAACAAAGTGATGAGGG CCAGGCTGTACCTCCTGCAGCAGATAGGACCCAACTCTTTCCTGATTGGAGGAGACAGTCCAGACAATAA ATACCGCGTGTTTATTGGGCCACAGAACTGCAGCTGTGGGCGTGGGACATTCTGCATTCACCTCTTGTTT GTCATGCTCCGGGTGTTCCAGCTAGAACCCTCCGACCCCATGCTATGGAGAAAAACTTTAAAAAATTTTG AGGTTGAGAGTTTGTTCCAGAAATACCACAGTAGGCGTAGCTCAAGAATCAAAGCTCCATCTCGGAACAC CATCCAGAAGTTTGTCTCACGCATGTCAAACTGTCACACATTGTCATCGTCTAGCACATCCACATCTAGT TCAGAAAACAGCATCAAGGATGAAGAGGAGCAGATGTGTCCCATCTGCTTGTTGGGCATGCTGGATGAGG AGAGCCTGACTGTGTGTGAAGACGGCTGCAGGAACAAGCTGCACCACCACTGCATGTCAATCTGGGCAGA AGAATGTAGAAGAAATAGAGAACCGTTAATATGTCCCCTTTGTAGATCTAAGTGGAGATCCCATGACTTC TACAGCCATGAGTTATCAAGCCCTGTGGATTCCCCTACCTCCCTGCGAGGTGTACAGCAGCCCTCCTCCC CGCAACAGCCTGTGGCCGGATCACAGCGGAGGAATCAGGAAAGCAATTTTAACCTTACTCATTATGGAAC CCAGCAGATTCCTCCCGCTTACAAAGATTTAGCCGAGCCATGGATTCAGGCATTCGGAATGGAGCTCGTT GGCTGCTTATTCTCTAGAAACTGGAACGTAAGGGAGATGGCCCTTAGGCGCCTTTCCCACGATGTTAGCG GGGCCCTGTTGTTGGCGAACGGGGAGAGCACTGGAACTTCTGGAGGCGGCAGCGGAGGCAGCCTAAGTGC TGGCGCCGCCAGTGGGTCTTCCCAGCCCAGCATCTCAGGGGACGTGGTGGAGGCGTTCTGCAGCGTCCTG TCTATCGTGTGCGCTGACCCTGTCTACAAAGTGTACGTTGCTGCTTTAAAAACACTGAGAGCCATGCTGG TATACACTCCTTGCCACAGCCTGGCAGAAAGAATCAAACTTCAGAGACTTCTCCGGCCAGTGGTAGACAC CATCCTAGTGAAGTGCGCAGACGCCAATAGCCGCACGAGTCAGCTGTCCATATCAACACTGTTGGAGCTC TGCAAGGGCCAAGCGGGGGAGTTGGCAGTTGGACGAGAAATACTTAAAGCTGGATCCATCGGTGTTGGTG GCGTTGATTACGTCTTAAGTTGTATTCTTGGAAACCAAGCTGAATCAAACAACTGGCAAGAACTCCTGGG TCGCCTCTGTCTTATAGACAGATTGCTCTTGGAAATTTCTGCGGAATTTTATCCTCATATTGTCAGTACC GATGTCTCACAAGCCGAGCCTGTTGAAATCAGGTATAAGAAGCTGCTGTCCCTCTTAGCCTTTGCCTTGC AATCCATTGACAATTCCCACTCTATGGTCGGCAAACTCTCGCGGAGGATTTATCTGAGCTCAGCACGAAT GGTGACCACAGTGCCCCCCCTGTTTTCCAAGCTGGTAACCATGCTAAGTGCTTCTGGATCCTCCCACTTC GCCAGGATGCGCCGGCGTCTGATGGCCATTGCAGACGAGGTAGAAATTGCCGAGGTCATCCAGCTGGGTT CGGAAGACACTTTGGATGGCCAGCAGGACAGCTCGCAGGCATTGGCCCCTCCCCGCTATCCAGAGAGCAG CTCCCTTGAGCACACAGCCCATGTAGAGAAAACTGGAAAAGGATTAAAAGCTACGAGACTGAGTGCCAGC TCAGAGGACATTTCTGACAGACTGGCTGGCGTTTCTGTAGGACTTCCTAGTTCAGCAACAACAGAACAAC CAAAGCCAACAGTTCAAACAAAAGGCAGACCCCACAGTCAGTGTTTGAACTCCTCTCCTTTGTCTCCTCC TCAGTTAATGTTTCCAGCAATATCAGCCCCATGTTCATCTGCCCCGTCTGTCCCAGCTGGCTCTGTAACA GATGCTTCTAAGCATAGACCTCGGGCGTTTGTTCCCTGTAAAATACCCTCTGCGTCTCCCCAAACACAGC GCAAGTTTTCCCTACAGTTCCAGAGGACCTGCTCTGAGAACCGAGACTCAGAAAAACTCTCCCCAGTCTT TACTCAGTCGAGACCCCCACCCTCCAGTAACATACACAGGGCAAAGGCATCCCGACCCGTTCCGGGTAGC ACAAGCAAACTAGGGGATGCCTCAAAAAACAGCATGACACTCGACCTGAACAGTGCTTCCCAGTGTGATG ACAGCTTTGGCAGTGGCAGCAACAGTGGCAGCGCCGTCATACCCAGTGAGGAGACGGCATTCACCCCAGC AGAGGACAAGTGCAGGTTAGATGTCAACCCCGAGCTCAACTCCAGTATCGAGGACCTTCTTGAAGCATCT ATGCCTTCAAGTGACACAACAGTCACTTTCAAGTCCGAAGTCGCCGTCCTCTCTCCTGAGAAGGCCGAAA GTGATGACACCTACAAAGATGACGTCAATCACAATCAAAAGTGCAAAGAGAAGATGGAGGCTGAGGAAGA GGAGGCGCTCGCGATTGCCATGGCAATGTCGGCTTCTCAGGACGCCCTCCCCATAGTCCCTCAGCTGCAG GTGGAAAATGGAGAGGATATCATCATCATCCAGCAGGATACACCAGAAACCCTACCAGGGCATACCAAAG CGAACGAGCCCTACAGAGAAGACACCGAGTGGCTGAAAGGGCAGCAGATAGGCCTCGGAGCATTTTCTTC TTGTTACCAAGCACAGGATGTGGGCACTGGAACTTTAATGGCTGTGAAACAGGTGACCTACGTCAGAAAC ACATCTTCTGAGCAGGAGGAAGTGGTGGAAGCCTTGAGGGAAGAGATCAGGATGATGAGCCACCTCAACC ATCCGAACATCATCAGGATGCTGGGGGCCACGTGCGAGAAGAGCAACTACAACCTCTTCATCGAGTGGAT GGCGGGAGCCTCCGTGGCTCACCTCTTGAGTAAATATGGAGCTTTCAAGGAGTCAGTGGTCATTAACTAC ACAGAGCAGTTACTCCGTGGCCTTTCCTATCTCCACGAGAACCAGATCATTCACAGAGACGTCAAAGGGG CCAATCTGCTCATTGACAGCACCGGTCAGCGGCTGAGAATTGCAGACTTCGGTGCTGCCGCCAGGTTGGC ATCCAAAGGAACTGGTGCAGGAGAGTTCCAGGGACAGTTACTGGGGACAATTGCATTCATGGCGCCTGAG GTACTAAGAGGTCAGCAGTACGGTAGGAGCTGTGACGTCTGGAGTGTTGGCTGCGCCATTATAGAAATGG CCTGTGCAAAACCACCCTGGAACGCAGAAAAGCACTCCAATCATCTTGCTTTGATATTTAAGATTGCGAG TGCAACTACTGCACCATCCATCCCGTCACACCTGTCCCCGGGCCTGCGAGACGTGGCTCTGCGCTGTTTA GAACTTCAACCTCAGGACAGACCTCCGTCAAGAGAGCTACTGAAGCATCCTGTCTTCCGTACAACATGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208228 representing NM_053887 Red=Cloning site Green=Tags(s) MAAAAGDRASSSGFPGAAAASPEAGGGGGALQGSGAPAAGAGLLRETGSAGRERADWRRQQLRKVRSVEL DQLPEQPLFLTASPPCPSTSPSPEPADAAAGASGFQPAAGPPPPGAASRCGSHSAELAAARDSGARSPAG AEPPSAAAPSGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKG DGSEMSNLAAELQGEGQAGSAAPAPKGRRSPSPGSSPSGRSGKPESPGVRRKRVSPVPFQSGRITPPRRA PSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLF VMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNCHTLSSSSTSTSS SENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDF YSHELSSPVDSPTSLRGVQQPSSPQQPVAGSQRRNQESNFNLTHYGTQQIPPAYKDLAEPWIQAFGMELV GCLFSRNWNVREMALRRLSHDVSGALLLANGESTGTSGGGSGGSLSAGAASGSSQPSISGDVVEAFCSVL SIVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLEL CKGQAGELAVGREILKAGSIGVGGVDYVLSCILGNQAESNNWQELLGRLCLIDRLLLEISAEFYPHIVST DVSQAEPVEIRYKKLLSLLAFALQSIDNSHSMVGKLSRRIYLSSARMVTTVPPLFSKLVTMLSASGSSHF ARMRRRLMAIADEVEIAEVIQLGSEDTLDGQQDSSQALAPPRYPESSSLEHTAHVEKTGKGLKATRLSAS SEDISDRLAGVSVGLPSSATTEQPKPTVQTKGRPHSQCLNSSPLSPPQLMFPAISAPCSSAPSVPAGSVT DASKHRPRAFVPCKIPSASPQTQRKFSLQFQRTCSENRDSEKLSPVFTQSRPPPSSNIHRAKASRPVPGS TSKLGDASKNSMTLDLNSASQCDDSFGSGSNSGSAVIPSEETAFTPAEDKCRLDVNPELNSSIEDLLEAS MPSSDTTVTFKSEVAVLSPEKAESDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQ VENGEDIIIIQQDTPETLPGHTKANEPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRN TSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGASVAHLLSKYGAFKESVVINY TEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPE VLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCL ELQPQDRPPSRELLKHPVFRTTW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	RsrII-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053887
ORF Size	4479 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053887.1, NP_446339.1
RefSeq Size	5180 bp
RefSeq ORF	4482 bp
Locus ID	116667
UniProt ID	Q62925
MW	161.3 kDa
Gene Summary	kinase that has E3 ubiquitin ligase to mediate ubiquitination and degradation of ERK1/2 [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.