Map3k1 (NM_053887) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Mekk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208228 representing NM_053887 Red=Cloning site Blue=ORF Orange=Stop codon GCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTC AAGCTTAACTAGCTAGCGGACCGAC ATGGCGGCGGCGGCGGGCGATCGCGCCTCGTCGTCGGGATTCCCGGGCGCCGCGGCGGCGAGCCCCGAGG CGGGCGGCGGCGGCGGAGCTCTCCAGGGAAGCGGCGCGCCCGCAGCGGGCGCGGGGCTGCTGCGGGAGAC TGGCAGCGCGGGCCGCGAGCGCGCGGACTGGCGGCGGCAGCAGCTGCGCAAAGTAAGGAGTGTGGAGCTG GACCAGCTGCCGGAGCAGCCGCTCTTCCTCACCGCCTCGCCGCCCTGCCCATCTACTTCCCCGTCGCCGG AGCCCGCGGACGCGGCTGCAGGAGCGAGTGGCTTCCAGCCTGCGGCGGGACCGCCACCCCCGGGAGCAGC GAGTCGCTGCGGCTCCCACTCTGCCGAGCTGGCGGCCGCGCGGGACAGCGGCGCCCGGAGCCCCGCGGGG GCCGAGCCGCCCTCTGCAGCGGCCCCCTCCGGTCGAGAGATGGAGAATAAAGAAACCCTCAAAGGATTGC ACAAGATGGATGACCGCCCAGAGGAGCGAATGATCAGGGAGAAGCTCAAGGCGACCTGTATGCCTGCCTG GAAGCACGAGTGGTTAGAAAGGAGGAACAGGAGAGGCCCTGTGGTGGTGAAACCAATCCCTATTAAAGGA GATGGATCTGAAATGAGTAACTTGGCAGCTGAGCTCCAAGGAGAGGGCCAGGCAGGCTCTGCTGCACCAG CTCCCAAGGGCCGACGAAGCCCGTCTCCTGGAAGCTCCCCATCAGGGCGCTCAGGGAAGCCGGAATCCCC AGGAGTGAGACGGAAACGAGTGTCCCCAGTGCCTTTCCAGAGTGGCAGAATCACACCACCTCGAAGAGCC CCGTCGCCAGATGGCTTCTCACCGTACAGCCCAGAGGAGACGAGCCGCCGTGTGAACAAAGTGATGAGGG CCAGGCTGTACCTCCTGCAGCAGATAGGACCCAACTCTTTCCTGATTGGAGGAGACAGTCCAGACAATAA ATACCGCGTGTTTATTGGGCCACAGAACTGCAGCTGTGGGCGTGGGACATTCTGCATTCACCTCTTGTTT GTCATGCTCCGGGTGTTCCAGCTAGAACCCTCCGACCCCATGCTATGGAGAAAAACTTTAAAAAATTTTG AGGTTGAGAGTTTGTTCCAGAAATACCACAGTAGGCGTAGCTCAAGAATCAAAGCTCCATCTCGGAACAC CATCCAGAAGTTTGTCTCACGCATGTCAAACTGTCACACATTGTCATCGTCTAGCACATCCACATCTAGT TCAGAAAACAGCATCAAGGATGAAGAGGAGCAGATGTGTCCCATCTGCTTGTTGGGCATGCTGGATGAGG AGAGCCTGACTGTGTGTGAAGACGGCTGCAGGAACAAGCTGCACCACCACTGCATGTCAATCTGGGCAGA AGAATGTAGAAGAAATAGAGAACCGTTAATATGTCCCCTTTGTAGATCTAAGTGGAGATCCCATGACTTC TACAGCCATGAGTTATCAAGCCCTGTGGATTCCCCTACCTCCCTGCGAGGTGTACAGCAGCCCTCCTCCC CGCAACAGCCTGTGGCCGGATCACAGCGGAGGAATCAGGAAAGCAATTTTAACCTTACTCATTATGGAAC CCAGCAGATTCCTCCCGCTTACAAAGATTTAGCCGAGCCATGGATTCAGGCATTCGGAATGGAGCTCGTT GGCTGCTTATTCTCTAGAAACTGGAACGTAAGGGAGATGGCCCTTAGGCGCCTTTCCCACGATGTTAGCG GGGCCCTGTTGTTGGCGAACGGGGAGAGCACTGGAACTTCTGGAGGCGGCAGCGGAGGCAGCCTAAGTGC TGGCGCCGCCAGTGGGTCTTCCCAGCCCAGCATCTCAGGGGACGTGGTGGAGGCGTTCTGCAGCGTCCTG TCTATCGTGTGCGCTGACCCTGTCTACAAAGTGTACGTTGCTGCTTTAAAAACACTGAGAGCCATGCTGG TATACACTCCTTGCCACAGCCTGGCAGAAAGAATCAAACTTCAGAGACTTCTCCGGCCAGTGGTAGACAC CATCCTAGTGAAGTGCGCAGACGCCAATAGCCGCACGAGTCAGCTGTCCATATCAACACTGTTGGAGCTC TGCAAGGGCCAAGCGGGGGAGTTGGCAGTTGGACGAGAAATACTTAAAGCTGGATCCATCGGTGTTGGTG GCGTTGATTACGTCTTAAGTTGTATTCTTGGAAACCAAGCTGAATCAAACAACTGGCAAGAACTCCTGGG TCGCCTCTGTCTTATAGACAGATTGCTCTTGGAAATTTCTGCGGAATTTTATCCTCATATTGTCAGTACC GATGTCTCACAAGCCGAGCCTGTTGAAATCAGGTATAAGAAGCTGCTGTCCCTCTTAGCCTTTGCCTTGC AATCCATTGACAATTCCCACTCTATGGTCGGCAAACTCTCGCGGAGGATTTATCTGAGCTCAGCACGAAT GGTGACCACAGTGCCCCCCCTGTTTTCCAAGCTGGTAACCATGCTAAGTGCTTCTGGATCCTCCCACTTC GCCAGGATGCGCCGGCGTCTGATGGCCATTGCAGACGAGGTAGAAATTGCCGAGGTCATCCAGCTGGGTT CGGAAGACACTTTGGATGGCCAGCAGGACAGCTCGCAGGCATTGGCCCCTCCCCGCTATCCAGAGAGCAG CTCCCTTGAGCACACAGCCCATGTAGAGAAAACTGGAAAAGGATTAAAAGCTACGAGACTGAGTGCCAGC TCAGAGGACATTTCTGACAGACTGGCTGGCGTTTCTGTAGGACTTCCTAGTTCAGCAACAACAGAACAAC CAAAGCCAACAGTTCAAACAAAAGGCAGACCCCACAGTCAGTGTTTGAACTCCTCTCCTTTGTCTCCTCC TCAGTTAATGTTTCCAGCAATATCAGCCCCATGTTCATCTGCCCCGTCTGTCCCAGCTGGCTCTGTAACA GATGCTTCTAAGCATAGACCTCGGGCGTTTGTTCCCTGTAAAATACCCTCTGCGTCTCCCCAAACACAGC GCAAGTTTTCCCTACAGTTCCAGAGGACCTGCTCTGAGAACCGAGACTCAGAAAAACTCTCCCCAGTCTT TACTCAGTCGAGACCCCCACCCTCCAGTAACATACACAGGGCAAAGGCATCCCGACCCGTTCCGGGTAGC ACAAGCAAACTAGGGGATGCCTCAAAAAACAGCATGACACTCGACCTGAACAGTGCTTCCCAGTGTGATG ACAGCTTTGGCAGTGGCAGCAACAGTGGCAGCGCCGTCATACCCAGTGAGGAGACGGCATTCACCCCAGC AGAGGACAAGTGCAGGTTAGATGTCAACCCCGAGCTCAACTCCAGTATCGAGGACCTTCTTGAAGCATCT ATGCCTTCAAGTGACACAACAGTCACTTTCAAGTCCGAAGTCGCCGTCCTCTCTCCTGAGAAGGCCGAAA GTGATGACACCTACAAAGATGACGTCAATCACAATCAAAAGTGCAAAGAGAAGATGGAGGCTGAGGAAGA GGAGGCGCTCGCGATTGCCATGGCAATGTCGGCTTCTCAGGACGCCCTCCCCATAGTCCCTCAGCTGCAG GTGGAAAATGGAGAGGATATCATCATCATCCAGCAGGATACACCAGAAACCCTACCAGGGCATACCAAAG CGAACGAGCCCTACAGAGAAGACACCGAGTGGCTGAAAGGGCAGCAGATAGGCCTCGGAGCATTTTCTTC TTGTTACCAAGCACAGGATGTGGGCACTGGAACTTTAATGGCTGTGAAACAGGTGACCTACGTCAGAAAC ACATCTTCTGAGCAGGAGGAAGTGGTGGAAGCCTTGAGGGAAGAGATCAGGATGATGAGCCACCTCAACC ATCCGAACATCATCAGGATGCTGGGGGCCACGTGCGAGAAGAGCAACTACAACCTCTTCATCGAGTGGAT GGCGGGAGCCTCCGTGGCTCACCTCTTGAGTAAATATGGAGCTTTCAAGGAGTCAGTGGTCATTAACTAC ACAGAGCAGTTACTCCGTGGCCTTTCCTATCTCCACGAGAACCAGATCATTCACAGAGACGTCAAAGGGG CCAATCTGCTCATTGACAGCACCGGTCAGCGGCTGAGAATTGCAGACTTCGGTGCTGCCGCCAGGTTGGC ATCCAAAGGAACTGGTGCAGGAGAGTTCCAGGGACAGTTACTGGGGACAATTGCATTCATGGCGCCTGAG GTACTAAGAGGTCAGCAGTACGGTAGGAGCTGTGACGTCTGGAGTGTTGGCTGCGCCATTATAGAAATGG CCTGTGCAAAACCACCCTGGAACGCAGAAAAGCACTCCAATCATCTTGCTTTGATATTTAAGATTGCGAG TGCAACTACTGCACCATCCATCCCGTCACACCTGTCCCCGGGCCTGCGAGACGTGGCTCTGCGCTGTTTA GAACTTCAACCTCAGGACAGACCTCCGTCAAGAGAGCTACTGAAGCATCCTGTCTTCCGTACAACATGGT AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	RsrII-MluI
ACCN	NM_053887
Insert Size	4482 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053887.1, NP_446339.1
RefSeq Size	5180 bp
RefSeq ORF	4482 bp
Locus ID	116667
UniProt ID	Q62925
Gene Summary	kinase that has E3 ubiquitin ligase to mediate ubiquitination and degradation of ERK1/2 [RGD, Feb 2006]

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