Srd5a2 (NM_022711) Rat Tagged ORF Clone
CAT#: RR208105
- TrueORF®
Srd5a2 (Myc-DDK-tagged ORF) - Rat steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (Srd5a2), (10 ug)
"NM_022711" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | S5AR 2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208105 representing NM_022711
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGATTGTCTGCCATCAGGTCCCGGTGCTGGCAGGTAGCGCCACATTGGCCACTATGGGGACCCTGA TCCTGTGCTTAGGGAAACCCGCCAGTTACGGGAAACACACAGAGAGTGTGTCGTCGGGAGTTCCCTTCCT GCCGGCACGCATCGCCTGGTTCCTGCAGGAGTTGCCTTCCTTTGTGGTGTCGGTAGGGATGCTCGCTTGG CAGCCGCGCTCCCTCTTCGGACCGCCCGGGAATGTCCTGCTGGCTCTCTTCTCTGCACATTACTTCCACA GGACATTTATTTACTCGTTGCTCACAAGAGGGAGGCCTTTCCCAGCGGTGCTGTTTTTGAGAGCCACTGC CTTCTGCATAGGGAACGGACTCCTTCAAGCCTACTACCTGGTTTACTGCGCAGAATACCCCGAGGAGTGG TACACAGATGTGCGGTTTAGCTTTGGTGTCTTCCTGTTTATTCTGGGGATGGGAATCAACATCCACAGTG ACTACACCCTGCGCCAGCTCAGGAAGCCTGGAGAAGTCATCTATAGGATTCCTCGAGGTGGCTTGTTTAC GTATGTCTCTGGAGCCAATTTCCTGGGCGAGATTATTGAATGGATTGGCTACGCCTTGGCCACGTGGTCC GTCCCAGCCTTCGCTTTCGCCTTTTTCACACTTTGTTTCCTGGGGATGCAAGCCTTTTACCACCACAGGT TCTACCTTAAGATGTTTAAGGATTACCCCAAATCTAGGAAAGCTCTCATTCCATTCATCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208105 representing NM_022711
Red=Cloning site Green=Tags(s) MQIVCHQVPVLAGSATLATMGTLILCLGKPASYGKHTESVSSGVPFLPARIAWFLQELPSFVVSVGMLAW QPRSLFGPPGNVLLALFSAHYFHRTFIYSLLTRGRPFPAVLFLRATAFCIGNGLLQAYYLVYCAEYPEEW YTDVRFSFGVFLFILGMGINIHSDYTLRQLRKPGEVIYRIPRGGLFTYVSGANFLGEIIEWIGYALATWS VPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_022711 |
ORF Size | 762 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022711.4, NP_073202.1 |
RefSeq Size | 1798 bp |
RefSeq ORF | 765 bp |
Locus ID | 64677 |
UniProt ID | P31214 |
MW | 28.8 kDa |
Gene Summary | enzyme which converts testosterone to dihydrotestosterone (DHT); also acts on progesterone and corticosterone; plays a central role in sexual differentiation and androgen physiology [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208105 | Srd5a2 (untagged ORF) - Rat steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (Srd5a2), (10 ug) |
CNY 3,990.00 |
|
RR208105L3 | Lenti ORF clone of Srd5a2 (Myc-DDK-tagged ORF) - Rat steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (Srd5a2), (10 ug) |
CNY 6,080.00 |
|
RR208105L4 | Lenti ORF clone of Srd5a2 (mGFP-tagged ORF) - Rat steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (Srd5a2), (10 ug) |
CNY 6,650.00 |