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Tie1 (NM_053545) Rat Tagged ORF Clone
CAT＃: RR208009
- TrueORF®
Tie1 (Myc-DDK-tagged ORF) - Rat tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10 ug)
"NM_053545" in other vectors (3)
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CNY 18,340.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208009 representing NM_053545
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTGGTGGGGATCCCCTTTCCTGCTCCCCATTCTTTTTCTGGCCTCTCATGTTGGTGCATCCGTGG ATTTAACACTGCTGGCCAATCTGCGTATCACCGAACCCCAGCGTTTCTTCCTGACCTGTGTGTCTGGTGA GGCGGGAGCAGGGAGGAGCTCCGACGTCTGGGGCCCGCCCCTGCTGCTGGAAAAGGATGATCGCATAGTG CGCACCTTCCCACCCGGGCAGCCCCTGCACCTCACTCGCAACGGTTCACACCAGGTCACGCTGCGGGGCT TCTCCAAGCCCTCGGATTTAGTAGGCGTCTTCTCCTGCGTGGGTGGAGCTGGCACGAGGCGCACTCGAGT CCTCTATGTGCACAACAGCCCGGGGGCACACCTGTTTCCAGACAAGGTCACACACACGGTGAACAAAGGT GATACCGCTGTGCTTTCTGCACGAGTGCACAAGGAAAAGCAGACTGATGTGATTTGGAAGAACAATGGAT CCTACTTCCATACCCTAGACTGGCATGAGGCCCATGATGGGCGGTTTCAACTGCAGCTCCAAAATGTACA GCCACCATCGAGTGGCATCTACAGCGCCACCTACCTAGAGGCCAGCCCCTTGGGCAGTGCCTTCTTTCGG CTCATCGTGCGAGGCTGTGAGGCTGGACGCTGGGGGCCGGGATGTGTCAAGGATTGCCCAGGCTGCCTGC ATGGAGGTGTCTGTCATGACCACGATGGCGAATGTGTGTGCCCCCCTGGATTCACTGGCACCCGCTGTGA GCAGGCCTGCAGAGAAGGTCGTTTTGGACAGAGCTGCCAGGAACAGTGCCCAGGCACAGCAGGCTGTCGG GGTCTCACCTTCTGCCTCCCAGATCCCTATGGCTGTTCTTGTGGATCTGGCTGGAGGGGAAGCCAGTGCC AGGAAGCATGTGCACCTGGTCATTTTGGGGCAGATTGTCGCCTCCAGTGTCAGTGTCAAAATGGTGGTAC TTGTGATCGGTTCAGCGGCTGCGTCTGCCCCTCCGGGTGGCATGGAGTCCACTGTGAAAAGTCAGACCGG ATCCCCCAGATCCTCAATGTGGCCACAGAGCTGGAGTTCAACTTAGGGACAATGCCCCGGATCAACTGTG CAGCTGCCGGGAATCCCTTCCCAGTACGGGGCAGCATGGAACTCCGCAAGCCAGACGGCACCATGCTCCT GTCTACCAAAGCCATTGTGGAGCCAGATAGGACCACAGCTGAGTTCGAGGTGCCCCGTTTGACTCTTGGG GACAGCGGGTTCTGGGAATGCCGTGTATCGACGTCTGGTGGCCAAGATAGCCGGCGCTTCAAGGTCAATG TCAAAGTACCCCCAGTGCCTCTGACTGCACCTCGGCTCCTGGCCAAGCAGAGCCGCCAGCTTGTGGTCTC CCCACTGGTCTCCTTCGGTGGGGATGGACCCATCTCCTCTGTCCGCCTGCACTACCGGCCCCAGGACAGC ATGATTACCTGGTCTGCCATTGTGGTGGATCCCAGTGAGAATGTGACATTAATGAACCTGAAGCCAAGGA CAGGATACAACGTTCGGGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAAGGAGCCTGGGGTCCTTCGAC TCTCATGACTACGGACTGTCCTGAGCCTTTGCTGCAGCCGTGGGTAGAGAGCTGGAACGTGGAGGGTCCT GACAGGCTACGGGTGAGCTGGTCTCTACCCTCGGTGCCACTGTCTGGGGATGGTTTCCTGCTGCGTCTGT GGGATGGGGCCCGGGGACAGGAGAGGCGGGAGAACATCTCATCCCCCCAGGCCCGCACTGCCCTCCTGAC TGGACTCACGCCTGGCACGCACTACCAACTGGATGTGCGGCTGTATCACTGCACCCTCCTGGGCCCTGCC TCACCCTCTGCGCATGTGCACCTGCCCCTCAGCGGGCCTCCAGCTCCCCGCCACCTCCGTGCCCAGGCCC TCTCAGACTCTGAGATCCGTCTGATGTGGCAGCATCCTGAGGCTCCGCCTGGACCTATATCCAAGTACAT TGTGGAGATTCAGGTGGCCGGGGGCTCAGGAGACCCTCAGTGGATGGATGTAGACAAACCCGAGGAGACG AGCACCACCGTCCGTGGTCTCAATGCCAGCACACGCTACCTCTTCCGTGTGCGGGCCAGTGTGCAGGGTC TCGGCGACTGGAGCAACACAGTGGAAGAGACCACGCTGGGTAATGGGCTGCAGAGTGCGAGCCCAGTCCA AGAGAGCCGGGTGGCTGAAGACGGTCTGGATCAGCAGCTGGTCCTGGCTGTGGTAGGCTCCGTCTCTGCC ACCTGCCTCACCATCCTGGCTGCCCTTTTAGCCCTGGTGTGTATCCGAAGAAGCTGCCTACATCGGAGAC ATACCTTCACCTACCAGTCAGGATCGGGTGAAGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACATT GACCCGACGGCCAAAGCCACAGCCTGAGCCTTTGAGTTACCCTGTGCTGGAGTGGGAGGACATCACCTTT GAGGACCTCATCGGGGAGGGGAACTTCGGCCAAGTGATCCGGGCCATGATCAAGAAGGACGGACTCAAGA TGAACGCAGCCATCAAGATGCTGAAAGAGTATGCGTCTGAAAATGACCACCGAGACTTTGCAGGGGAACT AGAAGTTCTGTGCAAACTAGGACACCACCCCAATATTATCAACCTCTTGGGGGCCTGTGAGAACCGAGGC TACTTGTATATTGCGATCGAATATGCCCCGTATGGAAACCTGCTAGATTTCCTGAGAAAGAGCAGGGTCC TGGAGACTGATCCAGCTTTTGCTCGAGAGCATGGAACAGCCTCCACACTCAGCTCCCGGCAGCTGCTGCG CTTTGCCAGTGATGCAGCCAACGGGATGCAGTACCTTAGTGAGAAGCAGTTCATTCACAGGGACCTGGCT GCCCGAAATGTTCTGGTCGGAGAGAACCTGGCCTCCAAGATTGCAGACTTTGGCCTTTCTCGGGGGGAGG AAGTATATGTGAAGAAGACGATGGGCCGTCTTCCTGTGCGTTGGATGGCCATTGAGTCCCTCAACTACAG CGTCTACACTACCAAGAGTGACGTCTGGTCATTTGGGGTCCTCCTCTGGGAGATAGTGAGCCTTGGAGGC ACGCCCTACTGTGGCATGACCTGTGCTGAGCTCTACGAGAAACTGCCTCAAGGCTATCGCATGGAGCAGC CTCGAAACTGTGATGATGAAGTGTACGAGCTGATGCGGCAGTGCTGGCGGGACCGTCCCTATGAGCGCCC CCCTTTTGCCCAGATTGCACTACAGTTGGGCCGCATGCTGGAAGCCAGGAAGGCCTACGTGAACATGTCA CTGTTTGAGAACTTTACCTATGCAGGCATCGATGCCACAGCTGAGGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208009 representing NM_053545
Red=Cloning site Green=Tags(s) MVWWGSPFLLPILFLASHVGASVDLTLLANLRITEPQRFFLTCVSGEAGAGRSSDVWGPPLLLEKDDRIV RTFPPGQPLHLTRNGSHQVTLRGFSKPSDLVGVFSCVGGAGTRRTRVLYVHNSPGAHLFPDKVTHTVNKG DTAVLSARVHKEKQTDVIWKNNGSYFHTLDWHEAHDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFR LIVRGCEAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCR GLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDR IPQILNVATELEFNLGTMPRINCAAAGNPFPVRGSMELRKPDGTMLLSTKAIVEPDRTTAEFEVPRLTLG DSGFWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFGGDGPISSVRLHYRPQDS MITWSAIVVDPSENVTLMNLKPRTGYNVRVQLSRPGEGGEGAWGPSTLMTTDCPEPLLQPWVESWNVEGP DRLRVSWSLPSVPLSGDGFLLRLWDGARGQERRENISSPQARTALLTGLTPGTHYQLDVRLYHCTLLGPA SPSAHVHLPLSGPPAPRHLRAQALSDSEIRLMWQHPEAPPGPISKYIVEIQVAGGSGDPQWMDVDKPEET STTVRGLNASTRYLFRVRASVQGLGDWSNTVEETTLGNGLQSASPVQESRVAEDGLDQQLVLAVVGSVSA TCLTILAALLALVCIRRSCLHRRHTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITF EDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRG YLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLA ARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGG TPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMS LFENFTYAGIDATAEEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053545 |
| ORF Size | 3411 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053545.1, NP_445997.1 |
| RefSeq Size | 3848 bp |
| RefSeq ORF | 3414 bp |
| Locus ID | 89806 |
| MW | 125.2 kDa |
| Gene Summary | essential for vascular development and remodeling in the embryo; may mediate maintenance and repair of the adult vascular system [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208009 | Tie1 (untagged ORF) - Rat tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10 ug) |
CNY 18,340.00 |
|
| RR208009L3 | Lenti ORF clone of Tie1 (Myc-DDK-tagged ORF) - Rat tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10 ug) |
CNY 19,760.00 |
|
| RR208009L4 | Lenti ORF clone of Tie1 (mGFP-tagged ORF) - Rat tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10 ug) |
CNY 21,760.00 |
