Tie1 (NM_053545) Rat Untagged Clone

CAT#: RN208009

Tie1 (untagged ORF) - Rat tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10 ug)



  "NM_053545" in other vectors (3)

CNY 18,340.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN208009 representing NM_053545
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCTGGTGGGGATCCCCTTTCCTGCTCCCCATTCTTTTTCTGGCCTCTCATGTTGGTGCATCCGTGG
ATTTAACACTGCTGGCCAATCTGCGTATCACCGAACCCCAGCGTTTCTTCCTGACCTGTGTGTCTGGTGA
GGCGGGAGCAGGGAGGAGCTCCGACGTCTGGGGCCCGCCCCTGCTGCTGGAAAAGGATGATCGCATAGTG
CGCACCTTCCCACCCGGGCAGCCCCTGCACCTCACTCGCAACGGTTCACACCAGGTCACGCTGCGGGGCT
TCTCCAAGCCCTCGGATTTAGTAGGCGTCTTCTCCTGCGTGGGTGGAGCTGGCACGAGGCGCACTCGAGT
CCTCTATGTGCACAACAGCCCGGGGGCACACCTGTTTCCAGACAAGGTCACACACACGGTGAACAAAGGT
GATACCGCTGTGCTTTCTGCACGAGTGCACAAGGAAAAGCAGACTGATGTGATTTGGAAGAACAATGGAT
CCTACTTCCATACCCTAGACTGGCATGAGGCCCATGATGGGCGGTTTCAACTGCAGCTCCAAAATGTACA
GCCACCATCGAGTGGCATCTACAGCGCCACCTACCTAGAGGCCAGCCCCTTGGGCAGTGCCTTCTTTCGG
CTCATCGTGCGAGGCTGTGAGGCTGGACGCTGGGGGCCGGGATGTGTCAAGGATTGCCCAGGCTGCCTGC
ATGGAGGTGTCTGTCATGACCACGATGGCGAATGTGTGTGCCCCCCTGGATTCACTGGCACCCGCTGTGA
GCAGGCCTGCAGAGAAGGTCGTTTTGGACAGAGCTGCCAGGAACAGTGCCCAGGCACAGCAGGCTGTCGG
GGTCTCACCTTCTGCCTCCCAGATCCCTATGGCTGTTCTTGTGGATCTGGCTGGAGGGGAAGCCAGTGCC
AGGAAGCATGTGCACCTGGTCATTTTGGGGCAGATTGTCGCCTCCAGTGTCAGTGTCAAAATGGTGGTAC
TTGTGATCGGTTCAGCGGCTGCGTCTGCCCCTCCGGGTGGCATGGAGTCCACTGTGAAAAGTCAGACCGG
ATCCCCCAGATCCTCAATGTGGCCACAGAGCTGGAGTTCAACTTAGGGACAATGCCCCGGATCAACTGTG
CAGCTGCCGGGAATCCCTTCCCAGTACGGGGCAGCATGGAACTCCGCAAGCCAGACGGCACCATGCTCCT
GTCTACCAAAGCCATTGTGGAGCCAGATAGGACCACAGCTGAGTTCGAGGTGCCCCGTTTGACTCTTGGG
GACAGCGGGTTCTGGGAATGCCGTGTATCGACGTCTGGTGGCCAAGATAGCCGGCGCTTCAAGGTCAATG
TCAAAGTACCCCCAGTGCCTCTGACTGCACCTCGGCTCCTGGCCAAGCAGAGCCGCCAGCTTGTGGTCTC
CCCACTGGTCTCCTTCGGTGGGGATGGACCCATCTCCTCTGTCCGCCTGCACTACCGGCCCCAGGACAGC
ATGATTACCTGGTCTGCCATTGTGGTGGATCCCAGTGAGAATGTGACATTAATGAACCTGAAGCCAAGGA
CAGGATACAACGTTCGGGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAAGGAGCCTGGGGTCCTTCGAC
TCTCATGACTACGGACTGTCCTGAGCCTTTGCTGCAGCCGTGGGTAGAGAGCTGGAACGTGGAGGGTCCT
GACAGGCTACGGGTGAGCTGGTCTCTACCCTCGGTGCCACTGTCTGGGGATGGTTTCCTGCTGCGTCTGT
GGGATGGGGCCCGGGGACAGGAGAGGCGGGAGAACATCTCATCCCCCCAGGCCCGCACTGCCCTCCTGAC
TGGACTCACGCCTGGCACGCACTACCAACTGGATGTGCGGCTGTATCACTGCACCCTCCTGGGCCCTGCC
TCACCCTCTGCGCATGTGCACCTGCCCCTCAGCGGGCCTCCAGCTCCCCGCCACCTCCGTGCCCAGGCCC
TCTCAGACTCTGAGATCCGTCTGATGTGGCAGCATCCTGAGGCTCCGCCTGGACCTATATCCAAGTACAT
TGTGGAGATTCAGGTGGCCGGGGGCTCAGGAGACCCTCAGTGGATGGATGTAGACAAACCCGAGGAGACG
AGCACCACCGTCCGTGGTCTCAATGCCAGCACACGCTACCTCTTCCGTGTGCGGGCCAGTGTGCAGGGTC
TCGGCGACTGGAGCAACACAGTGGAAGAGACCACGCTGGGTAATGGGCTGCAGAGTGCGAGCCCAGTCCA
AGAGAGCCGGGTGGCTGAAGACGGTCTGGATCAGCAGCTGGTCCTGGCTGTGGTAGGCTCCGTCTCTGCC
ACCTGCCTCACCATCCTGGCTGCCCTTTTAGCCCTGGTGTGTATCCGAAGAAGCTGCCTACATCGGAGAC
ATACCTTCACCTACCAGTCAGGATCGGGTGAAGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACATT
GACCCGACGGCCAAAGCCACAGCCTGAGCCTTTGAGTTACCCTGTGCTGGAGTGGGAGGACATCACCTTT
GAGGACCTCATCGGGGAGGGGAACTTCGGCCAAGTGATCCGGGCCATGATCAAGAAGGACGGACTCAAGA
TGAACGCAGCCATCAAGATGCTGAAAGAGTATGCGTCTGAAAATGACCACCGAGACTTTGCAGGGGAACT
AGAAGTTCTGTGCAAACTAGGACACCACCCCAATATTATCAACCTCTTGGGGGCCTGTGAGAACCGAGGC
TACTTGTATATTGCGATCGAATATGCCCCGTATGGAAACCTGCTAGATTTCCTGAGAAAGAGCAGGGTCC
TGGAGACTGATCCAGCTTTTGCTCGAGAGCATGGAACAGCCTCCACACTCAGCTCCCGGCAGCTGCTGCG
CTTTGCCAGTGATGCAGCCAACGGGATGCAGTACCTTAGTGAGAAGCAGTTCATTCACAGGGACCTGGCT
GCCCGAAATGTTCTGGTCGGAGAGAACCTGGCCTCCAAGATTGCAGACTTTGGCCTTTCTCGGGGGGAGG
AAGTATATGTGAAGAAGACGATGGGCCGTCTTCCTGTGCGTTGGATGGCCATTGAGTCCCTCAACTACAG
CGTCTACACTACCAAGAGTGACGTCTGGTCATTTGGGGTCCTCCTCTGGGAGATAGTGAGCCTTGGAGGC
ACGCCCTACTGTGGCATGACCTGTGCTGAGCTCTACGAGAAACTGCCTCAAGGCTATCGCATGGAGCAGC
CTCGAAACTGTGATGATGAAGTGTACGAGCTGATGCGGCAGTGCTGGCGGGACCGTCCCTATGAGCGCCC
CCCTTTTGCCCAGATTGCACTACAGTTGGGCCGCATGCTGGAAGCCAGGAAGGCCTACGTGAACATGTCA
CTGTTTGAGAACTTTACCTATGCAGGCATCGATGCCACAGCTGAGGAGGCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053545
Insert Size 3414 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053545.1, NP_445997.1
RefSeq Size 3848 bp
RefSeq ORF 3414 bp
Locus ID 89806
Gene Summary essential for vascular development and remodeling in the embryo; may mediate maintenance and repair of the adult vascular system [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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