Lig3 (NM_001012011) Rat Tagged ORF Clone
CAT#: RR207667
- TrueORF®
Lig3 (Myc-DDK-tagged ORF) - Rat ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_001012011" in other vectors (3)
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CNY 12,160.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR207667 representing NM_001012011
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTTGGCCTTTAAGACCCTCTTCCCGAGAAACCTCTGTGCCCTTGGCAGAAAAGAACTGTGCCTGT TTTCAGAACAGCATCACTGGCCTGCCATAAGACAGTTCAGCCAGTGGTCGGAAACAAATCTCCTTTGTGG GTGCTGCCTCCTCCAGAGAAGAAAGCCTGTCCTATCTTTCCAGAGAGGTCATCTAAGACCACGTGCCACC CACCTTATTTCGTGGTCAGGGTCGCATGTGGGACTCTGTACTGGCCCCTGTGAGATGGCCGAACAGCGGT TCTGTGTGGACTATGCCAAGCGGGGCACAGCTGGCTGCAAGAAATGCAAGGAGAAGATTGTAAAGGGCGT ATGCCGCATTGGCAAAGTGGTACCCAATCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCAC ATTAAGTGCATGTTTGAGAAACTGGAGCGGGCGCGGGCCACCACAAAAAAAATTGAAGACCTCACAGAGC TGGAAGGCTGGGAAGAGCTGGAAGATAATGAAAAGGAACAGATAAGCCAGCACATTGCAGACCTGTCTTC CAAGACAGCTGCCACACCGAAGAAGAAAGCTACTGTCCAGGCTAAGCTGACAACCACTGGCCAAGTGACA TCACCAGTGAAAGGCGCTTCGTTTATTACCAGTACCAATCCCCGGAAATTTTCTGGCTTTTCAGCAGCCA AACCGAACAACTCTGAGCAAGACCCCTCAAGCCCTGCTCCTAAGACCAGTCTGTCCGCAAGTAAATGTGA CCCTAAGCACAAAGATTGTCTACTACGAGAATTCCGGAAGCTGTGTGCCATGGTAGCTGAAAATCCTAGC TATAACACCAAGACCCAGATCATCCACGACTTCTTGCAGAAAGGCTCCACAGGAGATGGCTTCCGAGGCG ATGTGTACCTGACAGTGAAGCTGCTGCTGCCTGGTGTCATCAAGAGTGTGTACAACTTGAATGATAAGCA GATAGTGAAACTGTTCAGCCGAATTTTTAACTGCAACCCAGATGATATGGCCCGGGACCTAGAACAGGGT GACGTGTCCGAGACGATCAGAGTCTTCTTTGAGCAGAGCAAGTCTTTTCCCCCAGCTGCCAAAAGCCTCC TCACCATCCAGGAGGTGGATGCATTTCTTCTGCACCTCTCCAAGCTCACCAAAGAGGATGAGCAGCAGCA GGCCCTGCAGGACATTGCTTCCAGGTGTACAGCCAATGACCTTAAGTGCATTATCCGGCTGATCAAACAC GATCTGAAGATGAACTCGGGTGCAAAACACGTGTTAGATGCCCTTGACCCCAATGCTTATGAAGCCTTCA AAGCATCACGCAACCTGCAGGATGTGGTGGAGCGGGTCCTTCACAATGAGCAGGAGGTGGAGAAGGACCC AGGCCGGCGTCGGGCTCTGAGTGTCCAGGCCTCATTGATGACACCTGTGCAACCCATGCTGGCTGAGGCC TGCAAGTCCATTGAGTATGCAATGAAGAAGTGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGTG AGCGAGTCCAGGTGCATAAGAAGGGAGACCATTTCAGCTACTTCAGCCGCAGTCTCAAGCCTGTCTTGCC TCACAAGGTGGCCCACTTTAAGGACTATATTCCCAAAGCGTTTCCTGGGGGTCAAAGCATGATCTTGGAC TCTGAAGTGCTCCTGATTGATAACAACACAGGCAAACCACTGCCCTTTGGGACCCTGGGAGTACACAAGA AAGCTGCTTTCCAGGATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTT GATGGACAGGCCTCTCTGTGAGCGACGGAAGTTTCTTCATGATAACATGGTTGAAATCCGTAACCGGATC ATGTTCTCAGAAATGAAGCAAGTCACAAAAGCTTCAGACCTGGCTGACATGATAAACCGGGTGATCCGAG AGGGCCTGGAAGGTTTGGTGCTAAAGGATGTGAAGGGTACATATGAGCCTGGAAAACGACACTGGCTGAA AGTTAAGAAAGACTATTTGAACGAGGGGGCCATGGCTGATACAGCTGATCTGGTGGTTCTTGGGGCCTTC TATGGGCAAGGGAGCAAAGGTGGAATGATGTCCATCTTCCTCATGGGCTGCTATGACCCCGACAGCCAGA AGTGGTGCACTGTTACAAAATGTGCTGGAGGCCATGACGATGCCACACTTGCCCGCCTGCAGAAAGAGCT AGATATGGTGAAGATTAGCAAGGATCCTAGCAAGATACCCAGCTGGCTGAAGATCAACAAGATCTACTAT CCTGACTTTATCGTCCCAGACCCAAAGAAAGCTGCTGTGTGGGAGATCACAGGAGCAGAATTCTCTCGAT CTGAGGCGCATACTGCTGATGGGATCTCCATCCGATTTCCTCGATGCACCCGAATCCGGGATGACAAGGA CTGGAAATCTGCCACTAACCTCCCCCAACTCAAGGAACTGTACCAGCTGTCCAAAGACAAGGCAGACTTC GCTGTGGTGGCTGGAGATGAGGGGAGCTCCACTACTGGAGGCAGCAATGGTGAGAATGAGGGCACTGCTG GGTCTACTGTGCCCCGCAAGGGCCCCAAGGGACCCCCCAGTAAGTCCTCTGCCAGTGCCAAGAAGACTGA ACAGAAGCTGAATGATCCCAGTAGCAGAGGTGGAGAGAAGCTGGCTGTGAAGTCTTCTCCTGTCAAAGTG GGGATGAAGAGGAAAGCTGCTGATGAGACCCCAGGCCTGACAAAGAGGCGGCGGGCCAGCCGGCAAAGAG GAAGGAGAGCTATGCGAACAGGCAGGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207667 representing NM_001012011
Red=Cloning site Green=Tags(s) MTLAFKTLFPRNLCALGRKELCLFSEQHHWPAIRQFSQWSETNLLCGCCLLQRRKPVLSFQRGHLRPRAT HLISWSGSHVGLCTGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYH IKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQISQHIADLSSKTAATPKKKATVQAKLTTTGQVT SPVKGASFITSTNPRKFSGFSAAKPNNSEQDPSSPAPKTSLSASKCDPKHKDCLLREFRKLCAMVAENPS YNTKTQIIHDFLQKGSTGDGFRGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNCNPDDMARDLEQG DVSETIRVFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKH DLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNEQEVEKDPGRRRALSVQASLMTPVQPMLAEA CKSIEYAMKKCPNGMFSEIKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILD SEVLLIDNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIRNRI MFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAF YGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATLARLQKELDMVKISKDPSKIPSWLKINKIYY PDFIVPDPKKAAVWEITGAEFSRSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADF AVVAGDEGSSTTGGSNGENEGTAGSTVPRKGPKGPPSKSSASAKKTEQKLNDPSSRGGEKLAVKSSPVKV GMKRKAADETPGLTKRRRASRQRGRRAMRTGRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001012011 |
ORF Size | 2829 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001012011.1, NP_001012011.1 |
RefSeq Size | 3874 bp |
RefSeq ORF | 2832 bp |
Locus ID | 303369 |
MW | 105.4 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN207667 | Lig3 (untagged ORF) - Rat ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 12,160.00 |
|
RR207667L3 | Lenti ORF clone of Lig3 (Myc-DDK-tagged ORF) - Rat ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 13,780.00 |
|
RR207667L4 | Lenti ORF clone of Lig3 (mGFP-tagged ORF) - Rat ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 15,200.00 |