Scap (NM_001100966) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207434 representing NM_001100966 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGACTGAAAGGCTTCGTGAGAAGATATCTCAGGCGTTCTACAACCATGGGCTGCTCTGCGCAT CCTACCCCATTCCCATCATCCTCTTCACAGGACTCTGCATCCTAGCCTGCTGCTACCCGCTGCTGAAGCT TCCCTTGCCTGGAACGGGACCCGTGGAATTCTCCACGCCTGTGAAGGGTTACTCGCCCCCGCCTGCGGAC TCTGACCACAAACAAGGAGAGCCCAGTGAGCAGCCAGAGTGGTATGTGGGTGCCCCCGTGGCATACATCC AGCAGATATTCGTGAAGTCATCAGTGTCTCCCTGGCACAGAAACCTTCTGGCAGTAGATGTGTTCCGGTC ACCTCTGTCCCGAGCATTCCAACTGGTGGAAGAGATCCGGAACCATGTGCTGAGAGACAGCTCAGGGACC AAGAGCCTGGAGGAAGTTTGCCTGCAGGTGACAGACCTGCTGCCAGGCCTCAGGAAACTCCGGAGCCTAC TTCCCGAACATGGCTGCCTGCTGCTGTCACCTGGGAACTTCTGGCAGAATGACTGGGAAAGATTCCATGC TGACCCTGACATCATTGGAACCATCCATCAGCATGAGCCTAAAACCCTACAGACATCAGCCACACTCAAA GACTTGCTGTTCGGTGTTCCTGGGAAGTACAGTGGGGTCAGCCTCTACACGAGGAAGAGGATGGTCTCAT ACACCATCACCCTGGTCTTCCAGCGCTACCATGCCAAGTTTCTGAGCAGCCTCCGTGCCCGGCTCATGCT TCTGCACCCCAGCCCCAACTGCAGCCTCCGAGCAGAGAACCTGGTGCATGTGCACTTCAAAGAGGAGATT GGCATTGCCGAGCTCATCCCCCTCGTGACCACCTACATCATCCTGTTTGCCTACATCTACTTCTCCACAC GCAAGATCGACATGGTCAAGTCCAAGTGGGGCCTCGCCCTGGCAGCCGTGGTCACAGTGCTTAGCTCGCT GCTCATGTCTGTGGGGCTCTGCACTCTCTTCGGCCTGACGCCCACACTCAATGGCGGCGAGATTTTCCCA TATCTGGTGGTGGTTATTGGGCTAGAGAATGTGTTGGTGCTCACCAAGTCAGTGGTATCAACTCCAGTGG ACCTTGAGGTGAAGCTTCGAATTGCACAAGGCTTAAGCAGTGAGAGCTGGTCCATCATGAAGAACGTAGC AACTGAACTGGGCATCATCCTCATTGGCTACTTCACCCTTGTGCCTGCCATCCAAGAGTTCTGCCTCTTT GCTGTGGTGGGCCTGGTGTCTGACTTCTTCCTCCAGATGCTGTTCTTCACCACCGTGCTGTCCATCGACA TTCGCCGGATGGAGCTAGCAGACCTGAACAAGCGGCTGCCCCCTGAGTCCTGCCTGCCCTCAGCCAAGCC TGTGGGGAGGCCAGCCCGATATGAGAGACAGCTAGCTGTACGGCCGTCCACACCACACACCATCACATTG CAACCATCTTCCTTCCGAAACCTGCGGCTTCCCAAAAGGCTGCGTGTCATCTACTTCCTGGCCCGCACTC GCCTGGCACAGCGCCTCATCATGGCTGGTACAGTTGTCTGGATTGGCATCCTGGTATATACAGACCCGGC AGGGCTGCGCACCTACCTCGCTGCCCAGGTGACAGAACAGAGCCCACTGGGTGAGGGTTCCCTGGGGCCC ATGCCTGTGCCTAGTGGAGTGCTGCCTGCCAGCCACCCGGACCCTGCCTTCTCCATCTTCCCACCTGATG CTCCTAAACTGCCAGAGAACCAGACGTTGCCAGGTGAGCTGCCTGAGCATGCCGTTCCAGCAGAGGGCGT CCAGGACAGCCGAGCCCCAGAGGTGACTTGGGGGCCCGAGGATGAGGAGCTGTGGAGGAAATTGTCCTTC CGCCACTGGCCCACACTCTTCAACTACTATAATATCACACTGGCCAAAAGGTACATCAGCCTGCTGCCTG TCATCCCTGTCACACTACACCTGAATCCACGGGAGGCTCTGGAGGGGCGACACCCTCAGGATGGCCGCAC TGCCTGGGCCCCACCAGAGCCTTTGCCTGCTGGCCTGTGGGAGACCGGACCTAAGGGGCCAGGTGGAACA CAGACCCATGGCGACATTACCTTGTACAAGGTGGCTGCACTTGGCCTGGCAGCGGGCATTGTCCTAGTGC TGCTGCTGCTCTGCCTCTACCGGGTGCTCTGCCCGCGAAACTACGGGCAGCCGGGTGGTGGTGCGGGCAG GCGGAGGCGCGGAGAGCTGCCTTGCGATGACTATGGCTACGCACCGCCTGAGACGGAGATAGTGCCGCTG GTGCTGCGAGGGCACCTCATGGACATCGAGTGTCTGGCTAGCGATGGGATGCTCCTGGTGAGCTGCTGCC TGGCTGGCCAAGTCTGCGTGTGGGATGCACAGACCGGGGACTGCCTCACTCGCATCCCGCGCCCTGGGCC ACGCCGGGACAGCTGCGGAGGCGGAGCTTTTGAAGCTCAGGAGAACTGGGAAAGACTGTCTGATGGGGGC AAAGCTAGCCCGGAAGAGCCTGGCGACAGCCCTCCGCTGCGACGCCGCCCTCGAGGGCCTCCACCGCCTT CCCTCTTTGGGGACCAGCCAGACCTCACCTGCTTAATCGACACCAACTTCTCAGTGCAGCTGCCCCCAGA GCCCACTCAGCCCGAGCCTCGGCACCGGGCGGGCTGTGGCCGCTCTAGAGACTCTGGTTACGACTTCAGC CGTCTGGTGCAGCGTGTGTACCAAGAGGAAGGCCTGGCTGCTGTGCACATGTCGGCCCTGCGCCCACCCT CCCCGGGACCTCCCCTGCCCCAGGCCTCTCAAGAAGAGGGGACTGCTCCCGAGAAGGGCTCCCCCCCTCT GGCCTGGGCCCCCAGCACAGCCGGTTCCATCTGGAGCTTAGAGTTGCAAGGCAGTCTCATCGTGGTTGGG CGAAGCAGCGGCCGGCTGGAGGTGTGGGATGCCATTGAGGGCGTGCTCTGCTGCAGCAATGAGGAGATCT CCTCAGGCATCACAGCCCTTGTCTTCTTAGACAGGAGGATTGTAGCTGCTCGGCTCAACGGTTCCCTCGA TTTCTTTTCCTTGGAGACCCACACTTCCCTCAGCCCCCTGCAGTTCAGAGGGACCCCAGGGAGAGGCAGT TCTCCTTCCTCGCCTGTGTACAGCAGCAGCAACACTGTGGCCTGTCACCTGACCCACACAGTCCCCTGTG CACACCAGAAACCCATCACAGCCCTGAGAGCAGCAGCGGGGCGCCTGGTGACAGGGAGCCAAGACCATAC TCTGAGAGTCTTCCGACTGGAGGATTCGTGTTGCCTCTTTACCCTGCAGGGCCACTCGGGGGCAATCACA ACTGTGTACATTGATCAGACCATGGTATTGGCCAGTGGAGGACAAGATGGAGCCATCTGCCTGTGGGATG TACTAACAGGCAGCCGGGTCAGCCATACATTTGCTCACCGTGGAGATGTCACCTCCCTCACCTGTACCAC TTCCTGTGTTATCAGTAGTGGCCTGGATGACTTCATCAACATCTGGGACCGAAGCACAGGCATCAAGCTG TACTCCATTCAGCAGGACCTGGGCTGTGGTGCAAGCTTGGGTGTCATCTCTGATAACCTTCTGGTGACCG GCGGCCAGGGATGTGTCTCCTTTTGGGACCTAAACTATGGGGACCTGTTACAGACAGTCTACTTGGGAAA GAACAGTGAAGCCCAGCCTGCCCGGCAGATTTTGGTGCTGGACAATGCTGCCATTGTCTGCAACTTTGGC AGTGAGCTCAGCCTAGTGTATGTGCCCTCTGTGCTGGAGAAACTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207434 representing NM_001100966 Red=Cloning site Green=Tags(s) MTLTERLREKISQAFYNHGLLCASYPIPIILFTGLCILACCYPLLKLPLPGTGPVEFSTPVKGYSPPPAD SDHKQGEPSEQPEWYVGAPVAYIQQIFVKSSVSPWHRNLLAVDVFRSPLSRAFQLVEEIRNHVLRDSSGT KSLEEVCLQVTDLLPGLRKLRSLLPEHGCLLLSPGNFWQNDWERFHADPDIIGTIHQHEPKTLQTSATLK DLLFGVPGKYSGVSLYTRKRMVSYTITLVFQRYHAKFLSSLRARLMLLHPSPNCSLRAENLVHVHFKEEI GIAELIPLVTTYIILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFP YLVVVIGLENVLVLTKSVVSTPVDLEVKLRIAQGLSSESWSIMKNVATELGIILIGYFTLVPAIQEFCLF AVVGLVSDFFLQMLFFTTVLSIDIRRMELADLNKRLPPESCLPSAKPVGRPARYERQLAVRPSTPHTITL QPSSFRNLRLPKRLRVIYFLARTRLAQRLIMAGTVVWIGILVYTDPAGLRTYLAAQVTEQSPLGEGSLGP MPVPSGVLPASHPDPAFSIFPPDAPKLPENQTLPGELPEHAVPAEGVQDSRAPEVTWGPEDEELWRKLSF RHWPTLFNYYNITLAKRYISLLPVIPVTLHLNPREALEGRHPQDGRTAWAPPEPLPAGLWETGPKGPGGT QTHGDITLYKVAALGLAAGIVLVLLLLCLYRVLCPRNYGQPGGGAGRRRRGELPCDDYGYAPPETEIVPL VLRGHLMDIECLASDGMLLVSCCLAGQVCVWDAQTGDCLTRIPRPGPRRDSCGGGAFEAQENWERLSDGG KASPEEPGDSPPLRRRPRGPPPPSLFGDQPDLTCLIDTNFSVQLPPEPTQPEPRHRAGCGRSRDSGYDFS RLVQRVYQEEGLAAVHMSALRPPSPGPPLPQASQEEGTAPEKGSPPLAWAPSTAGSIWSLELQGSLIVVG RSSGRLEVWDAIEGVLCCSNEEISSGITALVFLDRRIVAARLNGSLDFFSLETHTSLSPLQFRGTPGRGS SPSSPVYSSSNTVACHLTHTVPCAHQKPITALRAAAGRLVTGSQDHTLRVFRLEDSCCLFTLQGHSGAIT TVYIDQTMVLASGGQDGAICLWDVLTGSRVSHTFAHRGDVTSLTCTTSCVISSGLDDFINIWDRSTGIKL YSIQQDLGCGASLGVISDNLLVTGGQGCVSFWDLNYGDLLQTVYLGKNSEAQPARQILVLDNAAIVCNFG SELSLVYVPSVLEKLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100966
ORF Size	3828 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100966.1, NP_001094436.1
RefSeq Size	4301 bp
RefSeq ORF	3831 bp
Locus ID	301024
UniProt ID	A2RRU4
MW	139.5 kDa
Gene Summary	Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).[UniProtKB/Swiss-Prot Function]

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