Tarbp2 (NM_001034941) Rat Tagged ORF Clone

CAT#: RR206897

  • TrueORF®

Tarbp2 (Myc-DDK-tagged ORF) - Rat TAR (HIV-1) RNA binding protein 2 (Tarbp2), (10 ug)



  "NM_001034941" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC124970
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR206897 representing NM_001034941
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAAGAGGATCAGGGCTCCGGCTCTACTACAGGCTGCGGGCTGCCCAGCATAGAGCAAATGCTGG
CCGCCAACCCGGGCAAGACCCCGATCAGCCTTCTTCAGGAGTATGGGACCAGAATAGGGAAGACGCCCGT
GTACGACCTTCTCAAAGCCGAGGGCCAAGCCCATCAACCTAATTTCACCTTCCGGGTCACCGTTGGCGAC
ACCAGCTGCACTGGTCAGGGCCCCAGCAAGAAGGCAGCCAAGCACAAGGCAGCTGAGGTGGCCCTCAAAC
ACCTCAAAGGGGGGAGCATGCTGGAACCAGCCCTGGAGGACAGCAGTTCTTTTTCTCTCCTAGACTCTTC
AGTGCCTGAGGACACTGCTGTCATTGCTGCAGAAGCTGCTGCCCCTGTTCCATCTGCTTTACTAACCAGG
AGCCCTCCCATGGAGATGCAGCCCCCTGTCTCTCCTCAGCAGTCTGAGTGCAACCCTGTTGGTGCTCTGC
AGGAGCTGGTGGTGCAGAAAGGCTGGCGTTTGCCAGAGTACATGGTGACCCAGGAGTCTGGGCCTGCTCA
CCGCAAAGAGTTCACCATGACCTGCCGGGTGGAGCGTTTCATTGAGATTGGCAGTGGGACCTCCAAGAAG
CTGGCAAAGCGCAACGCGGCAGCTAAGATGCTGCTTCGAGTGCACACGGTACCTCTGGATGCCCGCGACG
GCAACGAGGCTGAACCTGATGACGATCATTTTTCCATTGGCGTGAGCTCCCGCCTGGATGGACTAAGGAA
CCGTGGGCCAGGCTGCACCTGGGATTCCTTACGGAATTCTGTGGGAGAAAAGATCCTGTCCCTTCGCAAT
TGCTCCGTGGGCTCTCTGGGGTCTTTGGGCTCTGCCTGCTGCAGCATCCTCAGTGAGCTCTCTGCGGAGC
AGGCTTTCCATGTCAGCTATCTGGATATTGAGGAACTGAGCCTGAGTGGGCTCTGCCAGTGTCTAGTGGA
GCTGTCCACGCAGCCAGCCACTGTGTGTTATGGTTCTGCCACTACCAGGGAGGCAGCCCGAGGTGATGCT
GCTCGCCGCGCACTACAGTACCTCAGGATCATGGCCGGTAGCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR206897 representing NM_001034941
Red=Cloning site Green=Tags(s)

MSEEDQGSGSTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGD
TSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSLLDSSVPEDTAVIAAEAAAPVPSALLTR
SPPMEMQPPVSPQQSECNPVGALQELVVQKGWRLPEYMVTQESGPAHRKEFTMTCRVERFIEIGSGTSKK
LAKRNAAAKMLLRVHTVPLDARDGNEAEPDDDHFSIGVSSRLDGLRNRGPGCTWDSLRNSVGEKILSLRN
CSVGSLGSLGSACCSILSELSAEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCYGSATTREAARGDA
ARRALQYLRIMAGSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001034941
ORF Size 1095 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001034941.1, NP_001030113.1
RefSeq Size 1495 bp
RefSeq ORF 1098 bp
Locus ID 363006
UniProt ID Q3SWU0
MW 38.9 kDa
Gene Summary Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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