Irs1 (NM_012969) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IRS1IRM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206729 representing NM_012969 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCCCTCCGGATACCGATGGCTTCTCAGACGTGCGCAAGGTGGGTTACCTGCGCAAACCCAAGA GTATGCATAAGCGCTTTTTCGTGCTGCGGGCGGCCAGCGAGGCCGGGGGCCCGGCGCGCCTGGAGTATTA TGAGAACGAGAAGAAGTGGCGGCACAAGTCGAGCGCCCCCAAACGCTCGATCCCCCTCGAGAGCTGTTTC AACATCAACAAGCGGGCTGACTCCAAGAACAAGCACCTGGTGGCTCTCTACACCCGAGACGAACACTTTG CCATTGCGGCGGATAGCGAGGCTGAACAAGACAGCTGGTACCAGGCTCTTCTGCAGCTGCATAATCGGGC AAAGGCCCACCATGACGGGGCTGGAGGAGGCTGCGGTGGTAGCTGCAGCGGCAGCTCTGGCGTCGGAGAG GCAGGGGAGGACTTGAGCTATGACACGGGCCCAGGACCCGCGTTCAAGGAGGTCTGGCAGGTTATCCTGA AACCCAAGGGCTTAGGTCAGACAAAGAACTTGATTGGTATCTACCGCCTCTGCCTGACCAGCAAGACCAT CAGCTTTGTGAAGCTCAACTCTGAGGCTGCCGCTGTGGTGCTGCAGCTGATGAACATCAGACGCTGTGGC CACTCAGAGAACTTCTTCTTCATCGAGGTGGGGCGGTCAGCTGTGACCGGGCCCGGCGAGTTCTGGATGC AAGTGGATGACTCCGTGGTGGCCCAGAACATGCATGAGACCATTCTAGAGGCCATGCGGGCCATGAGCGA TGAGTTTCGCCCGCGCACGAAAAGCCAATCTTCATCCAGTTGCTCCAACCCCATCAGTGTTCCCCTGCGC AGGCACCATCTCAACAATCCTCCGCCCAGCCAAGTGGGGCTGACTCGGAGATCTCGAACTGAGAGCATCA CTGCCACCTCCCCTGCCAGTATGGTGGGTGGGAAACCAGGTTCCTTCAGGGTGCGTGCCTCCAGCGATGG CGAAGGCACCATGTCCCGTCCAGCATCAGTGGATGGCAGTCCTGTGAGCCCTAGCACCAACAGGACCCAC GCCCATCGGCATCGAGGCAGCTCCAGGTTGCACCCCCCACTCAACCACAGCCGCTCCATCCCTATGCCTT CTTCACGATGCTCCCCTTCAGCCACCAGCCCAGTGAGCCTGTCATCCAGTAGTACCAGTGGCCACGGCTC CACTTCAGACTGTCTCTTCCCGAGGCGCTCTAGTGCTTCCGTGTCCGGTTCTCCTAGCGATGGCGGTTTC ATCTCTTCTGATGAGTATGGCTCTAGTCCCTGCGATTTCCGAAGTTCCTTCCGCAGTGTCACCCCAGATT CCCTGGGCCACACCCCACCAGCCAGGGGTGAGGAGGAGCTGAGCAACTATATCTGCATGGGTGGCAAGGG AGCCTCCACCTTGACAGCTCCCAATGGTCACTACATTTTGTCTAGGGGTGGCAACGGCCATCGCTACATC CCAGGTGCTACCATGGGGACAAGCCCGGCGCTGACTGGAGACGAAGCCGCTGGTGCAGCAGATCTGGATA ACCGGTTTCGGAAGAGAACTCACTCGGCTGGCACGTCCCCCACCATATCCCACCAGAAGACCCCCTCGCA GTCCTCAGTGGTTTCTATTGAGGAATATACAGAGATGATGCCCGCTGCCTACCCACCAGGAGGTGGCAGT GGAGGCCGACTGCCCGGCTACCGGCATTCCGCCTTCGTGCCCACCCACTCCTATCCCGAGGAGGGTCTAG AGATGCACCACTTGGAACGTCGTGGGGGCCACCACCGTCCAGACTCCTCCAACCTCCACACCGATGATGG CTACATGCCCATGTCTCCCGGAGTGGCTCCAGTGCCCAGCAACCGCAAAGGAAATGGGGACTATATGCCC ATGAGCCCCAAGAGTGTATCTGCCCCCCAGCAGATCATTAACCCCATCAGGCGCCACCCACAGAGAGTGG ACCCCAATGGCTACATGATGATGTCTCCCAGTGGTAGTTGCTCTCCTGACATTGGAGGTGGGTCTTGCAG CAGTAGCAGCATCAGCGCAGCCCCTTCTGGGAGCAGCTATGGGAAGCCATGGACAAACGGAGTAGGGGGG CACCATACCCATGCCCTTCCCCATGCCAAACCTCCTGTTGAGAGCGGTGGTGGTAAGCTCTTGCCTTGCA CTGGTGACTACATGAACATGTCGCCAGTGGGAGATTCCAACACCAGCAGCCCCTCAGAATGCTACTATGG CCCAGAAGATCCCCAGCACAAGCCTGTCCTCTCCTACTACTCATTACCAAGGTCCTTTAAGCACACCCAG CGCCCTGGGGAGCCAGAGGAGGGTGCCAGGCACCAGCATCTTCGTCTCTCTTCAAGCTCTGGACGCCTTC GCTATACCGCAACTGCCGAAGATTCCTCCTCTTCCACCAGCAGCGACAGCCTGGGTGGGGGTTACTGTGG GGCTAGGCCAGAGTCTAGCGTCACACATCCCCACCACCATGCCTTGCAGCCCCATCTGCCTCGAAAGGTA GACACAGCTGCACAGACCAACAGCCGCCTGGCTCGACCCACAAGGCTGTCCTTGGGGGATCCCAAGGCAA GCACTTTACCCCGGGTACGAGAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGTCTTCCCTGCACCCTCC CGAGCCCAAAAGCCCAGGAGAATATGTGAATATTGAATTCGGGAGTGGCCAGCCAGGCTATTTAGCTGGC CCTGCAACTTCCCGTAGCTCCCCTTCAGTTCGATGTCTACCCCAGCTCCACCCAGCTCCCAGAGAAGAGA CTGGCTCGGAAGAGTACATGAACATGGACTTGGGGCCAGGCCGGAGGGCAACCTGGCAGGAGAGTGGTGG AGTTGAGTTGGGCAGAGTAGGCCCTGCACCTCCAGGGGCTGCTTCCATTTGTAGGCCAACCCGGTCGGTG CCAAATAGCCGTGGTGATTACATGACCATGCAGATAGGTTGTCCTCGTCAAAGCTATGTGGATACCTCAC CAGTGGCCCCAGTCAGCTATGCTGACATGCGGACAGGCATTGCTGCAGAGAAGGTGAGCCTGCCCAGAAC CACAGGAGCTGCCCCCCCTCCATCCTCCACAGCCTCTGCTTCTGCTTCTGTTACACCTCAAGGGGCCGCT GAGCAGGCCGCTCACTCTTCCTTGCTGGGAGGCCCTCAGGGACCTGGGGGCATGAGCGCATTCACCAGGG TGAACCTAAGTCCCAACCATAACCAGAGTGCCAAAGTGATTCGTGCAGACACTCAAGGCTGCCGGAGGAG GCACAGCTCCGAGACCTTCTCGGCGCCTACGCGGGCTGCCAACACAGTGTCTTTTGGAGCAGGGGCTGCA GGAGGGGGCAGCGGTGGTGGCAGTGAGGATGTGAAACGCCACAGCTCTGCATCCTTTGAGAATGTGTGGC TGAGACCCGGGGATCTAGGGGGAGCATCCAAGGAGTCGGCTCCAGGGTGCGGGGCTGCCGGGGGATTGGA GAAGAGTCTTAACTATATAGACTTGGATTTGGTCAAGGATGTTAAGCAGCACCCTCAAGACTGCCCCTCT CAACAGCAGTCCCTGCCACCCCCTCCCCCTCACCAACCCTTAGGCAGCAATGAGGGCAGCTCCCCAAGAC GCTCCAGTGAGGATTTAAGCACCTATGCCAGCATCAACTTCCAGAAGCAACCAGAGGACCGTCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206729 representing NM_012969 Red=Cloning site Green=Tags(s) MASPPDTDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCF NINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKAHHDGAGGGCGGSCSGSSGVGE AGEDLSYDTGPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCG HSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRTKSQSSSSCSNPISVPLR RHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTH AHRHRGSSRLHPPLNHSRSIPMPSSRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGF ISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGASTLTAPNGHYILSRGGNGHRYI PGATMGTSPALTGDEAAGAADLDNRFRKRTHSAGTSPTISHQKTPSQSSVVSIEEYTEMMPAAYPPGGGS GGRLPGYRHSAFVPTHSYPEEGLEMHHLERRGGHHRPDSSNLHTDDGYMPMSPGVAPVPSNRKGNGDYMP MSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGSCSPDIGGGSCSSSSISAAPSGSSYGKPWTNGVGG HHTHALPHAKPPVESGGGKLLPCTGDYMNMSPVGDSNTSSPSECYYGPEDPQHKPVLSYYSLPRSFKHTQ RPGEPEEGARHQHLRLSSSSGRLRYTATAEDSSSSTSSDSLGGGYCGARPESSVTHPHHHALQPHLPRKV DTAAQTNSRLARPTRLSLGDPKASTLPRVREQQQQQQQQQQSSLHPPEPKSPGEYVNIEFGSGQPGYLAG PATSRSSPSVRCLPQLHPAPREETGSEEYMNMDLGPGRRATWQESGGVELGRVGPAPPGAASICRPTRSV PNSRGDYMTMQIGCPRQSYVDTSPVAPVSYADMRTGIAAEKVSLPRTTGAAPPPSSTASASASVTPQGAA EQAAHSSLLGGPQGPGGMSAFTRVNLSPNHNQSAKVIRADTQGCRRRHSSETFSAPTRAANTVSFGAGAA GGGSGGGSEDVKRHSSASFENVWLRPGDLGGASKESAPGCGAAGGLEKSLNYIDLDLVKDVKQHPQDCPS QQQSLPPPPPHQPLGSNEGSSPRRSSEDLSTYASINFQKQPEDRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012969
ORF Size	3705 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012969.1, NP_037101.1
RefSeq Size	5365 bp
RefSeq ORF	3708 bp
Locus ID	25467
UniProt ID	P35570
MW	131.2 kDa
Gene Summary	a docking protein; may act to link the insulin receptor kinase with enzymes regulating cellular growth and metabolism [RGD, Feb 2006]

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