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Elavl4 (NM_001077651) Rat Tagged ORF Clone
CAT＃: RR206728
- TrueORF®
Elavl4 (Myc-DDK-tagged ORF) - Rat ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) (Elavl4), (10 ug)
"NM_001077651" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HuD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206728 representing NM_001077651
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGGAATGGCTTGAAGATGATAATTAGCACCATGGAGCCTCAGGTGTCAAATGGACCGACATCCA ATACAAGCAATGGACCCTCCAGCAACAACAGAAACTGTCCTTCTCCCATGCAGACAGGCGCTGCCACAGA CGACAGCAAAACCAACCTCATCGTCAACTATTTACCCCAGAATATGACCCAAGAGGAATTCAGGAGTCTC TTTGGGAGCATTGGTGAAATCGAATCCTGCAAACTCGTGAGAGACAAAATTACAGGACAGAGTTTAGGGT ATGGATTTGTTAACTATATTGATCCAAAGGATGCAGAGAAAGCCATCAACACTTTAAATGGACTCAGACT CCAGACCAAAACCATAAAGGTGTCGTACGCCCGTCCGAGCTCGGCCTCAATCAGGGATGCTAACCTGTAC GTTAGTGGCCTTCCCAAGACCATGACCCAGAAGGAGCTGGAGCAGCTTTTCTCTCAGTACGGTCGCATCA TCACCTCACGCATCCTGGTTGATCAAGTCACAGGAGTCTCTAGAGGGGTGGGATTCATCCGCTTTGATAA GAGGATTGAGGCAGAAGAAGCCATCAAAGGGCTGAATGGCCAGAAGCCCAGCGGTGCTACAGAACCGATT ACTGTGAAGTTTGCCAACAACCCCAGCCAGAAGTCCAGCCAGGCCCTGCTCTCCCAGCTGTACCAGTCCC CCAACAGGCGATACCCTGGCCCTCTCCACCACCAGGCTCAAAGATTCAGGCTGGACAATTTGCTTAATAT GGCCTATGGCGTAAAGAGACTGATGTCTGGACCAGTCCCCCCTTCTGCTTGTCCCCCCAGGTTCTCCCCA ATCACCATTGACGGGATGACAAGTCTTGTGGGAATGAACATCCCTGGTCACACAGGGACAGGCTGGTGCA TCTTCGTCTATAACCTGTCCCCTGATTCTGATGAGAGTGTCCTCTGGCAGCTCTTTGGCCCCTTTGGCGC AGTGAACAACGTCAAGGTCATCCGTGACTTCAACACCAACAAATGCAAGGGATTCGGCTTTGTCACCATG ACCAACTACGATGAGGCAGCCATGGCCATCGCCAGCCTCAATGGTTACCGCCTGGGAGACAGAGTGTTGC AAGTTTCCTTTAAAACCAACAAAGCCCACAAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206728 representing NM_001077651
Red=Cloning site Green=Tags(s) MEWNGLKMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGAATDDSKTNLIVNYLPQNMTQEEFRSL FGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLY VSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPI TVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRLMSGPVPPSACPPRFSP ITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTM TNYDEAAMAIASLNGYRLGDRVLQVSFKTNKAHKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077651 |
| ORF Size | 1155 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001077651.2, NP_001071119.2 |
| RefSeq Size | 3873 bp |
| RefSeq ORF | 1158 bp |
| Locus ID | 432358 |
| UniProt ID | O09032 |
| MW | 42.4 kDa |
| Gene Summary | RNA-binding protein that is involved in the post-transcriptional regulation of mRNAs (PubMed:10982410, PubMed:16508003, PubMed:17577668). Plays a role in the regulation of mRNA stability, alternative splicing and translation (PubMed:10982410, PubMed:16508003, PubMed:17577668). Binds to AU-rich element (ARE) sequences in the 3' untranslated region (UTR) of target mRNAs, including GAP43, VEGF, FOS, CDKN1A and ACHE mRNA (PubMed:10982410). Many of the target mRNAs are coding for RNA-binding proteins, transcription factors and proteins involved in RNA processing and/or neuronal development and function (By similarity). By binding to the mRNA 3' UTR, decreases mRNA deadenylation and thereby contributes to the stabilization of mRNA molecules and their protection from decay (By similarity). Also binds to the polyadenylated (poly(A)) tail in the 3' UTR of mRNA, thereby increasing its affinity for mRNA binding (By similarity). Mainly plays a role in neuron-specific RNA processing by stabilization of mRNAs such as GAP43, ACHE and mRNAs of other neuronal proteins, thereby contributing to the differentiation of neural progenitor cells, nervous system development, learning and memory mechanisms (PubMed:10982410, PubMed:17577668). Involved in the negative regulation of the proliferative activity of neuronal stem cells and in the positive regulation of neuronal differentiation of neural progenitor cells (By similarity). Promotes neuronal differentiation of neural stem/progenitor cells in the adult subventricular zone of the hippocampus by binding to and stabilizing SATB1 mRNA (By similarity). Binds and stabilizes MSI1 mRNA in neural stem cells (By similarity). Exhibits increased binding to ACHE mRNA during neuronal differentiation, thereby stabilizing ACHE mRNA and enhancing its expression (By similarity). Protects CDKN1A mRNA from decay by binding to its 3' UTR (PubMed:16508003). May bind to APP and BACE1 mRNAS and the BACE1AS lncRNA and enhance their stabilization (By similarity). Plays a role in neurite outgrowth and in the establishment and maturation of dendritic arbors, thereby contributing to neocortical and hippocampal circuitry function (By similarity). Stabilizes GAP43 mRNA and protects it from decay during postembryonic development in the brain (PubMed:10982410, PubMed:17234598). By promoting the stabilization of GAP43 mRNA, plays a role in NGF-mediated neurite outgrowth (PubMed:10982410). Binds to BDNF long 3' UTR mRNA, thereby leading to its stabilization and increased dendritic translation after activation of PKC (PubMed:25692578). By increasing translation of BDNF after nerve injury, may contribute to nerve regeneration (By similarity). Acts as a stabilizing factor by binding to the 3' UTR of NOVA1 mRNA, thereby increasing its translation and enhancing its functional activity in neuron-specific splicing (By similarity). Stimulates translation of mRNA in a poly(A)- and cap-dependent manner, possibly by associating with the EIF4F cap-binding complex (By similarity). May also negatively regulate translation by binding to the 5'UTR of Ins2 mRNA, thereby repressing its translation (By similarity). Upon glucose stimulation, Ins2 mRNA is released form ELAVL4 and translational inhibition is abolished (By similarity). Also plays a role in the regulation of alternative splicing (By similarity). May regulate alternative splicing of CALCA pre-mRNA into Calcitonin and calcitonin gene-related peptide 1 (CGRP) by competing with splicing regulator TIAR for binding to U-rich sequences of CALCA pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206728 | Elavl4 (untagged ORF) - Rat ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) (Elavl4), (10 ug) |
CNY 3,990.00 |
|
| RR206728L3 | Lenti ORF clone of Elavl4 (Myc-DDK-tagged ORF) - Rat ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) (Elavl4), (10 ug) |
CNY 6,080.00 |
|
| RR206728L4 | Lenti ORF clone of Elavl4 (mGFP-tagged ORF) - Rat ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) (Elavl4), (10 ug) |
CNY 6,650.00 |
