Nedd4l (NM_001008300) Rat Tagged ORF Clone

CAT＃: RR206542

  • TrueORF®

Nedd4l (Myc-DDK-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 4-like (Nedd4l), (10 ug)

Datasheet
SDS
Plasmid Map

  "NM_001008300" in other vectors (3)

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CNY 12,350.00

货期*
7周
规格
    • 10 ug
Cited in 1 publication.

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Nedd4-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR206542 representing NM_001008300
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAGGGAGAATCCCGTATTCTCAGAGTAAAAGTTGTTTCTGGGATTGACCTCGCCAAAAAGGACA
TATTTGGAGCCAGTGACCCATACGTGAAGCTCTCGTTGTACGTAGCTGACGAGAACAGAGAACTCGCTCT
GGTTCAGACTAAGACAATTAAAAAGACGCTGAACCCAAAGTGGAATGAAGAGTTTTATTTTAGAGTAAAT
CCATCTAATCACAGGCTCTTATTTGAAGTATTTGACGAGAACAGATTGACACGAGACGACTTTCTGGGCC
AGGTGGACGTGCCTCTTAGTCACCTTCCGACAGAAGATCCAACCATGGAGAGACCCTATACATTTAAGGA
TTTTCTCCTGCGACCTAGAAGTCATAAATCACGCGTCAAGGGGTTTTTGAGGTTGAAAATGGCCTACATG
CCGAAAAACGGAGGTCAGGATGAAGAAAACAGCGAGCAGAGGGACGACATGGAGCATGGATGGGAAGTTG
TTGACTCAAACGACTCAGCTTCCCAGCACCAGGAGGAGCTCCCTCCTCCTCCCCTGCCACCAGGATGGGA
AGAGAAAGTGGACAATTTAGGCCGAACTTACTATGTCAACCACAACAACAGGAGTACGCAGTGGCACCGA
CCTAGCTTGATGGATGTATCGTCAGAGTCAGACAATAACATCAGGCAGATCAATCAGGAGGCCGCACACC
GGCGTTTCCGCTCTCGGAGGCACATTAGTGAAGACTTGGAGCCTGAGGCCTCTGAAGGTGGTGGAGAAGG
CCCTGAGCCTTGGGAGACCATTTCAGAGGAAGTGAACATGGCGGGAGATTCTCTCAGCCTGGCTCTACCC
CCACCTCCTGCCTCCCCAGTGTCAAGGACCAGCCCCCAGGAGCTGTCGGAAGAACTGAGCAGAAGGTTGC
AGATCACTCCGGACTCCAATGGGGAACAGTTCAGTGCTCTGATTCAGAGAGAGCCCTCGTCTAGGCTCCG
GTCCTGCAGTGTTACCGACACGGTTGCTGAACAAGCTCACCTTCCACCGCCCAGCACCCCAACTAGGCGA
GCCCGTTCGTCAACTGTCACGGGTGGTGAGGAATCAACGCCATCAGTGGCCTATGTACATACCACGCCGG
GCCTACCTTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGACGCACATACTATGTCAATCATAACAATCG
AACCACAACTTGGACTCGACCAATCATGCAGCTTGCAGAAGACGGTGCCTCTGGATCAGCCACAAACAGT
AACAACCACCTAGTCGAACCCCAGATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAACCTTATCTG
CCCCACTGGAGGGTGCCAAGGATTCACCCATACGCCGTGCTGTGAAAGATACCCTTTCCAATCCACAGTC
CCCTCAGCCATCACCTTACAACTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCCTGCCACCAGGC
TGGGAGATGAGGATAGCGCCAAACGGCCGACCCTTCTTCATTGACCATAACACAAAGACGACAACCTGGG
AAGATCCACGGCTGAAATTTCCAGTACATATGCGGTCAAAAGCATCTTTAAACCCCAATGACCTGGGCCC
TCTTCCTCCTGGCTGGGAAGAAAGGATCCACCTGGACGGCCGCACATTTTACATTGACCATAATAGCAAA
ATTACTCAGTGGGAAGATCCAAGACTACAGAATCCAGCCATAACTGGTCCGGCTGTGCCGTACTCCAGAG
AATTTAAGCAGAAATACGACTACTTTAGGAAGAAATTAAAGAAACCTGCTGATATTCCAAACAGGTTTGA
AATGAAACTCCACAGAAACAACATATTTGAAGAATCCTATCGGAGGATCATGTCTGTAAAGAGACCTGAC
GTCCTAAAGGCTAGACTGTGGATCGAGTTTGAATCAGAGAAAGGCCTGGACTATGGGGGCGTGGCCAGAG
AGTGGTTCTTCTTACTGTCCAAAGAGATGTTCAATCCCTACTATGGCCTCTTCGAGTACTCTGCCACGGA
CAACTACACACTTCAGATCAATCCCAACTCAGGCCTGTGTAACGAAGATCACTTATCCTATTTCACTTTC
ATTGGGAGAGTTGCTGGCCTGGCAGTGTTTCATGGGAAACTCTTAGATGGATTCTTCATTCGACCTTTCT
ACAAGATGATGCTGGGGAAGCAAATAACTCTGAATGACATGGAGTCTGTGGACAGCGAGTACTACAACTC
TTTGAAGTGGATCTTGGAAAACGACCCCACGGAACTTGACCTCATGTTCTGCATAGATGAAGAGAACTTT
GGGCAGACTTACCAAGTGGACCTGAAGCCCAATGGGTCAGAAATCATGGTAACCAATGAGAACAAGCGAG
AATATATTGACTTGGTCATCCAGTGGAGATTTGTGAACAGGGTCCAGAAGCAAATGAATGCCTTCTTGGA
GGGATTTACAGAACTTCTTCCAATCGACTTGATTAAAATTTTTGATGAAAATGAGCTGGAGTTGCTGATG
TGCGGCCTTGGTGATGTGGACGTGAATGATTGGAGACAGCACTCCATTTACAAGAACGGCTACTGCCCCA
ACCACCCTGTCATCCAGTGGTTCTGGAAGGCCGTGCTGCTGATGGATGCTGAGAAGCGGATCCGGTTACT
ACAGTTTGTCACAGGCACCTCCAGAGTACCCATGAATGGATTTGCCGAACTTTATGGTTCCAATGGTCCT
CAGCTGTTTACAATAGAGCAATGGGGTAGTCCTGAAAAACTGCCCAGAGCTCATACATGCTTTAACCGCC
TTGATTTACCTCCGTATGAAACCTTTGAAGATCTACGGGAGAAGCTTCTCATGGCCGTGGAGAATGCTCA
AGGCTTCGAAGGTGTGGAT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
>RR206542 representing NM_001008300
Red=Cloning site Green=Tags(s)

MEQGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVN
PSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYM
PKNGGQDEENSEQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRSTQWHR
PSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEASEGGGEGPEPWETISEEVNMAGDSLSLALP
PPPASPVSRTSPQELSEELSRRLQITPDSNGEQFSALIQREPSSRLRSCSVTDTVAEQAHLPPPSTPTRR
ARSSTVTGGEESTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNS
NNHLVEPQIRRPRSLSSPTVTLSAPLEGAKDSPIRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPG
WEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSK
ITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPD
VLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTF
IGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENF
GQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLM
CGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGP
QLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001008300
ORF Size 2889 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001008300.2, NP_001008301.2
RefSeq Size 4213 bp
RefSeq ORF 2892 bp
Locus ID 291553
MW 110.9 kDa
Gene Summary mouse homolog appears to be involved in binding and regulating the amiloride-sensitive epithelial sodium channel which plays a role in sodium homeostasis and control of blood volume and blood pressure [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone ,null, Frontiers in Physiology ,PubMed ID 29184507 [Nedd4l]

Other Versions

SKU Description Size Price
RN206542 Nedd4l (untagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 4-like (Nedd4l), (10 ug)
    • 10 ug

CNY 12,350.00

RR206542L3 Lenti ORF clone of Nedd4l (Myc-DDK-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 4-like (Nedd4l), (10 ug)
    • 10 ug

CNY 14,060.00

RR206542L4 Lenti ORF clone of Nedd4l (mGFP-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 4-like (Nedd4l), (10 ug)
    • 10 ug

CNY 15,490.00

You may also need
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RN206542

Nedd4l (untagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 4-like (Nedd4l), (10 ug)

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RR206542L3V

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RR206542L3

Lenti ORF clone of Nedd4l (Myc-DDK-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 4-like (Nedd4l), (10 ug)

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CNY 14,060.00

RR206542L4V

Lenti ORF particles, Nedd4l (GFP-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 4-like (Nedd4l), 200ul, >10^7 TU/mL

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RR206542L4

Lenti ORF clone of Nedd4l (mGFP-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 4-like (Nedd4l), (10 ug)

    • 10 ug

CNY 15,490.00

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