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Gpr149 (NM_138891) Rat Tagged ORF Clone
CAT＃: RR205861
- TrueORF®
Gpr149 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 149 (Gpr149), (10 ug)
"NM_138891" in other vectors (3)
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CNY 5,344.00
CNY 9,310.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ieda |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205861 representing NM_138891
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTTCTTCCTCAGCAACTTAACAAATGACTCTAGACTGTGGAAAGTAAGTCATAATTCTACGGACC TTATGAATTCGCCAGAAACTCTGACTCTTTCTCTCTTTTGCCTGATCTGTTTAATGACTCTCGTAGCTCT GGTAGGCAGCATTTTCTCACTGGTTTCTCTGCTCACCATGCAGTACAGAACTGTTGTGTCTATGCTTGTG ACCTCTTGGTCTGTGGATGATCTCTTGAGCGTCTTGTCAGTGGCCATCTTCATGGTTTTGCAGTGGCCAA GAGAGGCACCGGGCTACTTCCAGTCTCTGTGTACCACCTCTGCCTTACTGTATATGTGCCAGGGCCTCTC CAGCAACTTGAAGGCGACTCTTATAGTCTTCTACAACTTTTATACGATGCACAGGACAGTGGTGAGTCAG TCAAGCTCCTGGCGATCAGGACAGGTACTCGGTGTGGCTTTAACCGTGTGGGCAGTCAGCCTGCTGCTGG CCTCGCTCCCGCTGTGCGGCTGGGGTGTCTTCGTGCGCACGCCTTGGGGCTGCCTGACCGACTGCTCCAG CCCATACGTGCTGCTCCTCTTCGCGGTGTACGCTTCCGCTTTCGGGCTCCTGGCGGTTCTTTCTGTCCCT CTCACTCACCAGCTGCTGTGTTCAGAGGAGCCGCCAAGACTCCATGCCAACTACCAGGAAATTTCTCGTG GCGCCTCTACTCCTGGGACCCCTGCTGCTGGGGGAAGAGTGCTCTGTCTGTTGCCAGAGGATGTTGAAAT CCCTGCTCTGCCAGGCACTGGGAGCTCCCTGAGCTCCGACATGGTGTTTGCACCGGGTCAGCCTGCTGCC TCTAGCGCTGGAGCCGGCAAGCGAGAGAATCTCTGGACTCCCCGTGGCTCGAGCAGCTTCCCCGTGAGCC TGGCACAGAAGCGCTTTGCTTTGATCCTAGCGCTGACTAAAGTCATCCTCTGGCTGCCTATGATGATCCA CATGGTGGTAAAACACGTGGTGGGGTTTCAGAGCCTTCCGGTCGATATGCTCAGCTTTCTACTAACCCTG CTGGCCAGCACTGTAACACCAGTGTTTGTCTTGTCTAAACGATGGGCCCACTTGCCTTGTGGCTGCATCA TCAACTGCCAGCCTGACACCTACTCCGTTGCATTCGATGGGAAAAAGTCAAAGAGGAAAGGCTTTGAATT CAATTTATCGTTCCAACAAAGTTATGGACTCTATAAGATGACACATGCAGATTACTATGACGATGATGAT GAAAATCCCATATCCTACCACAACCCAAAGAAATATGAGTGTGAAGCTACAAAGGAGCCTCGGGAAGACA ACCACGGTGTGTTCAATACCATCACAGTAGAAATCAGCACCACGCCACCGCTGGACAGCGCCACACTGAC CGGTGTCAACAAGTGCACCAACACGGATATCCCTGAGCCCAAACAGGCCGTGAGCGAAGAAAAGGGTGCC TTTTCTATTAAAACAGAATGTGCTATTAACTATGGAGAAGCCACCTCGTTTGAAGGTCCAGAGAGACGAC TGTCCCATGAGGAGACTCAGAAACCAGATCTTTCAGACTGGGAGTGGTGCAGGAGTAAGTCAGAACGAAC ACCTCGCCAGCGTTCTGGTGGTGGGCTGGCTATTCCCATTTGTGCGTTCCAAGGGACTGTGTCTCTCCAA GCACCAACTGGGAAAACCCTCTCTCTTTCTACTTATGAGGTCAGTGCAGAAGGGCAAAAGATAACCCCGC CCTCTAAGAAAATAGAAGTCTATCGATCTAAAAGTGTTGGCCATGAACCGAACTCAGAAGAGTCTCCCTC CACGTTTGCAGACACCAACGTAAAAATACACTTGGAAGTTCTTGAAATTTGTGACAACGATGAGGCTTTA GACACTGTGTCAATCATCAGCAACATCAGCCAGTCCTCTACAAAAGTTCGGTCTCCTTCTCTGCGCTACT CACGGAAGGAAAACAGGTTTGTCTCATGCGACTTAGGAGAAACTGCCTCCTACTCCCTCTTCTTACCCAC AAGTGATCCTGACGGAGATATCAACATCTCTATCCCAGACACAGTGGAGGCACACAGACAGAACAGCAGG CGGCAGCATCAAGACAGGGATGGCTACCAGGAAGAGATCCAGTTGTTAAACAAAGCCTACAGGAAAAGAG AAGCAGAAAGCAAGGGTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205861 representing NM_138891
Red=Cloning site Green=Tags(s) MSFFLSNLTNDSRLWKVSHNSTDLMNSPETLTLSLFCLICLMTLVALVGSIFSLVSLLTMQYRTVVSMLV TSWSVDDLLSVLSVAIFMVLQWPREAPGYFQSLCTTSALLYMCQGLSSNLKATLIVFYNFYTMHRTVVSQ SSSWRSGQVLGVALTVWAVSLLLASLPLCGWGVFVRTPWGCLTDCSSPYVLLLFAVYASAFGLLAVLSVP LTHQLLCSEEPPRLHANYQEISRGASTPGTPAAGGRVLCLLPEDVEIPALPGTGSSLSSDMVFAPGQPAA SSAGAGKRENLWTPRGSSSFPVSLAQKRFALILALTKVILWLPMMIHMVVKHVVGFQSLPVDMLSFLLTL LASTVTPVFVLSKRWAHLPCGCIINCQPDTYSVAFDGKKSKRKGFEFNLSFQQSYGLYKMTHADYYDDDD ENPISYHNPKKYECEATKEPREDNHGVFNTITVEISTTPPLDSATLTGVNKCTNTDIPEPKQAVSEEKGA FSIKTECAINYGEATSFEGPERRLSHEETQKPDLSDWEWCRSKSERTPRQRSGGGLAIPICAFQGTVSLQ APTGKTLSLSTYEVSAEGQKITPPSKKIEVYRSKSVGHEPNSEESPSTFADTNVKIHLEVLEICDNDEAL DTVSIISNISQSSTKVRSPSLRYSRKENRFVSCDLGETASYSLFLPTSDPDGDINISIPDTVEAHRQNSR RQHQDRDGYQEEIQLLNKAYRKREAESKGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138891 |
| ORF Size | 2190 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138891.1, NP_620246.1 |
| RefSeq Size | 3845 bp |
| RefSeq ORF | 2193 bp |
| Locus ID | 192251 |
| UniProt ID | Q924Y8 |
| MW | 81 kDa |
| Gene Summary | induced during early steps of astrocyte differentiation [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205861 | Gpr149 (untagged ORF) - Rat G protein-coupled receptor 149 (Gpr149), (10 ug) |
CNY 9,310.00 |
|
| RR205861L3 | Lenti ORF clone of Gpr149 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 149 (Gpr149), (10 ug) |
CNY 11,120.00 |
|
| RR205861L4 | Lenti ORF clone of Gpr149 (mGFP-tagged ORF) - Rat G protein-coupled receptor 149 (Gpr149), (10 ug) |
CNY 12,260.00 |
