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Large2 (NM_199107) Rat Tagged ORF Clone
CAT＃: RR205806
- TrueORF®
Gyltl1b (Myc-DDK-tagged ORF) - Rat glycosyltransferase-like 1B (Gyltl1b), (10 ug)
"NM_199107" in other vectors (3)
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CNY 8,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gyltl1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205806 representing NM_199107
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCTCGAGGGCGCCCCCGGGCACTGGGGGCCGCACTGCTGTTGTTGCTGCTGCTAGTGGTTGGCT TCTTCCTATTCGGCCGGGACCCGGAGTATGGACTAGGCACAACCGCCACCCTCGATGGAGACCCGTACGG GAGCCGCAACCGCTCCACCTCCAGCCTGCAGCTTCTACTGCCACCCAAGTGCGAGATGTTGCATGTGGCC ATCGTGTGTGCGGGATACAACTCCAGCCGAGAGATCATCACCCTAATGAAGTCCGTGCTATTCTACAGGA AAAATCCGCTGCACCTCCACCTGATAACCGATGCCGTAGCCAGAAACATCCTGGAGACACTCTTCCGAAC ATGGATGGTACCAGCTGTGGTGGTCAGCTTCTATGATGCTGAAGAGCTCAAGCCCCTGGTTTCCTGGATT CCCAACAAGCACTACTCTGGCCTCTATGGGCTCATGAAGCTAGTACTTCCCAGCGTCCTGCCTCTCAGCC TGGCCCGAGTCATTGTCCTGGACACCGATGTCACTTTCTCCTCTGACATTATGGAGCTCTGGGCACTCTT TGGTCATTTTTCTGACAAGCAGGTGGTCGGTCTTGTGGAGAACCAGAGCGACTGGTACCTGGGCAACCTC TGGAAGAACCATAGGCCCTGGCCTGCCTTGGGCAGGGGATTTAACACAGGTGTGATCCTGCTTTGGCTGG ACAGATTACAGCAGATTGGCTGGGAGCAGATGTGGAAGCTGACGGCCAAACGGGAGCTGCTTACTTTGAC TGCCACTTCCTTGGCTGACCAGGACATCTTCAATGCCGTCATCAAGGAGCATCCCGAGCTGGTGCATCCC CTGCCCTGTGTCTGGAACGTGCAGCTGTCAGACCACACTCTGGCTGAGCGCTGCTACTTGGAAGCAGCTG ACCTCAAAGTGATCCACTGGAATTCACCAAAGAAGCTTCGAGTGAAGAACAAGCATGCAGAATTCTTCCG TGATCTACACTTGACCTTCCTGGGGTTTGACGGGAAGCTACTGTGTAGAGAGCTCTTTGGTTGCCCCAAC CAGTTCCCTCCTGGGGTTGAGCAGTTGCAACAGGCCCTAGCACAGCTGGATGAGGAAGAGCCCTGCTTTG AGTTCCGCCAACAGCAGCTCACTGTGCACCGTGTACACATCACCTTTCTGTCTCACCAGCCGCCACCTCC TCGGCCTCACGATGTCACCTTGGTGGCCCAGCTCTCTATGGACCGGCTGCAGATGCTGGAAGCCCTGTGC AGGCACTGGCGGGGTCCCATGAGCCTGGCCTTGTACCTGACAGACGCAGAGGCTCAACAGTTCCTGCGTT TCGTGGAGACGTCACCAGTACTCTCTGCAAGGAAGGATGTGGCCTATCATGTAGTGTACCGGGACGGCCC CCTCTACCCAGTCAACCAGCTCCGAAATGTGGCCTTGGCCCAGGCTCTCACACCCTACGTCTTCCTCAGT GATATTGACTTCTTACCTGCCTACTCCCTCTATGACTACCTCAGGGCCTCTATTGAGCAACTGGCGCTGG GCAGGCGGCAGAGGAAGGCTGCTTTGGTAGTGCCTGCATTTGAGACCCTGCACTACCGTTTCAGCTTCCC AAACTCTAAGGCAGAGTTGTTGACCTTACTGGATGCTGGCTCTCTCCACACCTTTAGGTACCACGAGTGG CCACAGGGTCATGCATCCACAGACTATACCCGCTGGCGGGAAGCCCAGGCACCATACCGAGTGCAGTGGT CAGCTGACTATGAGCCTTACGTGGTGGTACCCCGTGACTGTCCCCGCTATGACCCTCGCTTTGTGGGCTT TGGCTGGAACAAGGTGGCCCATATCATAGAGCTGGATGCTCAGGAATATGAATTCCTGGTACTTCCTGAG GCCTTCTCTATCCACTTGCCCCATGCCCCAAGTCTGGACATCTCCCGCTTCCGCTCCAGCCCCACCTACC GCGACTGCCTCCAGGCCCTCAAGGAGGAGTTCCACCAGGACTTGTCTAGGCGCTATGGGTCTGCAGCCCT CAAATACCTCACTGCCCTGCAGCAGTCCCGAAGCCGGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205806 representing NM_199107
Red=Cloning site Green=Tags(s) MLPRGRPRALGAALLLLLLLVVGFFLFGRDPEYGLGTTATLDGDPYGSRNRSTSSLQLLLPPKCEMLHVA IVCAGYNSSREIITLMKSVLFYRKNPLHLHLITDAVARNILETLFRTWMVPAVVVSFYDAEELKPLVSWI PNKHYSGLYGLMKLVLPSVLPLSLARVIVLDTDVTFSSDIMELWALFGHFSDKQVVGLVENQSDWYLGNL WKNHRPWPALGRGFNTGVILLWLDRLQQIGWEQMWKLTAKRELLTLTATSLADQDIFNAVIKEHPELVHP LPCVWNVQLSDHTLAERCYLEAADLKVIHWNSPKKLRVKNKHAEFFRDLHLTFLGFDGKLLCRELFGCPN QFPPGVEQLQQALAQLDEEEPCFEFRQQQLTVHRVHITFLSHQPPPPRPHDVTLVAQLSMDRLQMLEALC RHWRGPMSLALYLTDAEAQQFLRFVETSPVLSARKDVAYHVVYRDGPLYPVNQLRNVALAQALTPYVFLS DIDFLPAYSLYDYLRASIEQLALGRRQRKAALVVPAFETLHYRFSFPNSKAELLTLLDAGSLHTFRYHEW PQGHASTDYTRWREAQAPYRVQWSADYEPYVVVPRDCPRYDPRFVGFGWNKVAHIIELDAQEYEFLVLPE AFSIHLPHAPSLDISRFRSSPTYRDCLQALKEEFHQDLSRRYGSAALKYLTALQQSRSRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199107 |
| ORF Size | 2070 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199107.1, NP_954538.1 |
| RefSeq Size | 2455 bp |
| RefSeq ORF | 2073 bp |
| Locus ID | 311202 |
| UniProt ID | Q6P7A1 |
| MW | 79.3 kDa |
| Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205806 | Gyltl1b (untagged ORF) - Rat glycosyltransferase-like 1B (Gyltl1b), (10 ug) |
CNY 8,840.00 |
|
| RR205806L3 | Lenti ORF clone of Gyltl1b (Myc-DDK-tagged ORF) - Rat glycosyltransferase-like 1B (Gyltl1b), (10 ug) |
CNY 10,640.00 |
|
| RR205806L4 | Lenti ORF clone of Gyltl1b (mGFP-tagged ORF) - Rat glycosyltransferase-like 1B (Gyltl1b), (10 ug) |
CNY 11,780.00 |
