Ptprj (NM_017269) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DEP-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205764 representing NM_017269 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCGGGGAAGCCCGGGGCGGGCGGAGCGGGGACTAGGCGGACCGGCTGGCGGAGAAGGAGGCGGA GGCGTCGGCTGGAGGCGGAGACCAGGGCGCCCGGCTTCGGGCACACGGCGGGGCGCGTCCCGGGCACGTT CCAGGGCGCGCAGGGCATGAAGCCCGCGGCGCGGGAGACGCGGACACCCCCGCGCTCGCCCGGGCTCCGC TGGGCGCTGCTGCCGCTGCTGCTGTTGCTACGCCAGGGCCAGGTTGTGTGCGCAGGTGCGGCGCCCAGTC CTGTATTTGACGTTGAAGCTGTCACCAGCCCTACCAGTGTGGTATTAACGTGGAAACACAATGATTCAGC CACTTCAGAATATAAGATAAATGAGGGAAACACGCTGCGGTATACTGTTAAAAACCAGACATCATTTAAC ATCACAGGCTTAAGCCCAGCTACTTCCTACAAATTCTCCATCACCTTGGGAACAGTCAATGAGACCTCAG GAAAGCCCACATACAAAAATATCACAACAGAGCCCTGGCCAGTGTCGGATCTCCAAGTCGCCTACATCGG TGTGACACAGGCTCTTCTCGCCTGGAGCAATGCAAACGGCACCGCTTCTTACCGGATGCAGATTGTAGAG CTGACCACAAATTCATCAGGTGGTATTTCAGATCTGAAGCCGGGGACCCATAAGAGCTTGGCTGTCCAAG GATCGAATGAGACCCAGCATGACTTATGGGTCACAGAAGGCGTCTCGGATGCCAATGGCACCGAGGGAAG CCCAGTGGCCAACATATCCCAACTACATAAGAATTCTCTTGCCTCTGCAGACCCACCCTCTGCCCGGGAT CCCTCCCTCACAGAGGTCTTGCTCACTGAGCTAAAGCCTGATACTCAGTACAAAGTCACCATTTATTCTC AAGCAGCAGATGGCACAGAAGGACAGCCTGGGAACAAAGTGTTTAAAACAAATCCCATCCAAGTTTCTGA CATCCGAGCTGTGAACATCAGTGACTCGAACATGACCCTGACCTGGAAAAGCAACAATAATGAGTCCCAT GCTTCATTTACCTACAAAATATACGTGGCTGGGGGTTCCGACTCCATCAACGAAACTGTCAATGAGACTC AGGCCGTCATCCGTGGACTCAGCTCCAGTACCTTGTACAACATCACAGTGCTTCCTTTCCTGGGTCAGAC CGCGGGTATACCAGGCTTCCTCCAAGTGTACACTTCCCCCCGTCCTGTTTCTGACTTCCGAGTGACAAAT GTCAGCCTGAGGGAAATCGGTTTGGCTTGGAGAAGCAATGACTCGGAGTCATTCGAGATTTTCATCACGC AGGAGGGAAGTGAGAAACGTTGGAATGCTTCGACGGGAGACCTGAGCTATATCGTTGAAAATTTAAAGCC TGGGACCAGTTACCAATTTGAAATATTCCCACGAGGACCAAATGGGACTGAAGGGCCATCCCAGACAGTT GTTGGTAGAACTGACTGCAGTGCTGTGACTGACATCCGCGTGGTCAGCGTTAGCACCACTGAAATACAGC TGGAGTGGCAGAATACAGACAGTGCTTCTGGATACACTTACCATTTAGTTCTAGAGTCTGAGAATGGCTC CATCAAGACCAACAGTTCTCAGAAATGGATCACAGTGGGGGGCCTTACCCCAGGCACCTTATACAATGTT ACAATCTTTCCAGAAGTGGACCAGATGGAGGGCAACTCCAGCTCTATTACCCAGTACACAAGGCCCAGCA ATGTGTCCTACATTGAAGTAAACACCAACACCACCGTGGCAGCCATCCAGTGGAAGAACCTGGACGCAGC CTCTGCTTCCTACTCCTACTCCGTTCTTATCTTGAAGGCTGGAGATGGCAGCAATGTAACCAGCAGGGTC AGAGACATCCCTTCTGTCACCATCCCTGGGCTAATCCCCGGCGTCTCTTACGAAGTGAAGATCTTTACAA AGATTAGGAATACAGAGGTTGGGAATGAGGTACCTGGTCAGAAGCTGTTCTGTATGGAACCTGCACAAGT GGATTCCTTACACTGTGAAGTGGTCCCGAAGGAGCCAGCGCTGGTTCTGAAGTGGGCCTGCCCCCCTGGC ATGAACTCAGGCTTTGAACTGGGGGTCAGGAGTGATGCTTGGGACAATATGACACACTTGGAGAACTGCA CTTTGGACAATGACACAGAGTGCAGGACAGAAGTCACCTATTTGAATTTTTCTACCTCGTACAACATCAG CATTGCCACCTTGTCATGTGGAAAGATGGCATTGCCCACCCAGAGCACCTGCACCACTGGCATCACAGAC CCACCTCCTCCAGATGGATCCCCTAATATTACATCGGTCAGTCACAATTCAGTAAAGGTGAAGTTCAGTG GTTTTGAAGCCAGCCATGGACCTATCAAAGCCTATGCTGTCCTTCTCACCACAGGGGAAGCTGGCCAACC CTCCACAGATGTTTTGAAGTACACATATGAAGATTTCAAAAAGGGAGCCTCCGATACTTATGTGACATAC CTCATAAGGATAGAAGAGAAGGGACAGTCTCAGGGCTTGTCTGAAGCATTGAACTATGAGATTGATGTGG GGAACCAATCCACTACCCTCGGCTACTACAACGGGAGGCTAGAGCCTCTGGGCTCCTACCGGGCTTGCGT TGCTGGCTTTACCAATATTACCTACAACCTTCAGAACGATGGCCTTATTAATGGGGATGAGAGCTATGTA TCTTTCAGTCCGTATTCAGAGGCCGTGTCCTTGCCTCAAGATCCAGGTGTCATCTGCGGAGCGGTGTTCG GATGTATCTTTGGTGCCCTGGCCATTGTGGCTGTGGGAGGCTTCATCTTCTGGAGAAAGAAAAGGAAAGA TGCCAAGAATAATGAAGTGTCCTTTTCTCAAATTAAATCCAAGTTAATCCGAGTGGAGAATTTTGAGGCC TACTTTAAGAAACAGCAAGCCGACTCCAACTGTGGGTTTGCAGAGGAATATGAGGATCTGAAGCTGATTG GAATAAGTTTACCTAAATATGCAGCGGAAATAGCTGAAAACAGGGGGAAGAACCGCTACAACAACGTCCT GCCCTATGATATTTCTCGAGTCAAACTTTCAGTCCAGACCCATTCGACAGACGACTACATCAATGCCAAC TATATGCCTGGCTACCATTCCAAGAAAGATTTCATTGCCACACAAGGACCTTTACCCAACACTTTGAAAG ATTTTTGGCGTATGGTTTGGGAGAAAAACGTATATGCCATTGTTATGCTGACCAAATGTGTGGAGCAGGG AAGGACCAAATGTGAAGAGTACTGGCCTTCCAAGCAGGCTCAGGACTACGGGGACATAACTGTGGCAATG ACATCAGAAGTTGTTCTTCCGGAATGGACCATCAGAGATTTTGTGGTGAAAAATATGCAGAGTAGTGAGA GTCATCCTCTGCGGCAGTTCCATTTCACCTCCTGGCCTGACCATGGTGTTCCTGACACCACCGACCTGCT CATCAACTTTCGGTACCTGGTCCGGGATTACATGAAGCAGATCCCCCCTGAGTCACCAATCCTGGTGCAT TGCAGTGCTGGGGTTGGAAGGACGGGCACTTTCATTGCTATTGATCGCCTGATCTATCAGATAGAGAACG AGAACACCGTGGATGTGTATGGCATTGTCTACGATCTTCGGATGCACAGGCCTCTGATGGTACAGACAGA GGACCAGTATGTTTTCCTCAATCAGTGTGTGTTGGATATTATCAGAGCCCAGAAAGACTCAAAAGTTGAC CTCATCTATCAGAACACAACCGCAATGACAATCTATGAAAACCTCGAGCCAGTGAGCATGTTTGGAAAGA CTAATGGTTACATCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205764 representing NM_017269 Red=Cloning site Green=Tags(s) MSPGKPGAGGAGTRRTGWRRRRRRRRLEAETRAPGFGHTAGRVPGTFQGAQGMKPAARETRTPPRSPGLR WALLPLLLLLRQGQVVCAGAAPSPVFDVEAVTSPTSVVLTWKHNDSATSEYKINEGNTLRYTVKNQTSFN ITGLSPATSYKFSITLGTVNETSGKPTYKNITTEPWPVSDLQVAYIGVTQALLAWSNANGTASYRMQIVE LTTNSSGGISDLKPGTHKSLAVQGSNETQHDLWVTEGVSDANGTEGSPVANISQLHKNSLASADPPSARD PSLTEVLLTELKPDTQYKVTIYSQAADGTEGQPGNKVFKTNPIQVSDIRAVNISDSNMTLTWKSNNNESH ASFTYKIYVAGGSDSINETVNETQAVIRGLSSSTLYNITVLPFLGQTAGIPGFLQVYTSPRPVSDFRVTN VSLREIGLAWRSNDSESFEIFITQEGSEKRWNASTGDLSYIVENLKPGTSYQFEIFPRGPNGTEGPSQTV VGRTDCSAVTDIRVVSVSTTEIQLEWQNTDSASGYTYHLVLESENGSIKTNSSQKWITVGGLTPGTLYNV TIFPEVDQMEGNSSSITQYTRPSNVSYIEVNTNTTVAAIQWKNLDAASASYSYSVLILKAGDGSNVTSRV RDIPSVTIPGLIPGVSYEVKIFTKIRNTEVGNEVPGQKLFCMEPAQVDSLHCEVVPKEPALVLKWACPPG MNSGFELGVRSDAWDNMTHLENCTLDNDTECRTEVTYLNFSTSYNISIATLSCGKMALPTQSTCTTGITD PPPPDGSPNITSVSHNSVKVKFSGFEASHGPIKAYAVLLTTGEAGQPSTDVLKYTYEDFKKGASDTYVTY LIRIEEKGQSQGLSEALNYEIDVGNQSTTLGYYNGRLEPLGSYRACVAGFTNITYNLQNDGLINGDESYV SFSPYSEAVSLPQDPGVICGAVFGCIFGALAIVAVGGFIFWRKKRKDAKNNEVSFSQIKSKLIRVENFEA YFKKQQADSNCGFAEEYEDLKLIGISLPKYAAEIAENRGKNRYNNVLPYDISRVKLSVQTHSTDDYINAN YMPGYHSKKDFIATQGPLPNTLKDFWRMVWEKNVYAIVMLTKCVEQGRTKCEEYWPSKQAQDYGDITVAM TSEVVLPEWTIRDFVVKNMQSSESHPLRQFHFTSWPDHGVPDTTDLLINFRYLVRDYMKQIPPESPILVH CSAGVGRTGTFIAIDRLIYQIENENTVDVYGIVYDLRMHRPLMVQTEDQYVFLNQCVLDIIRAQKDSKVD LIYQNTTAMTIYENLEPVSMFGKTNGYIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017269
ORF Size	3867 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017269.2, NP_058965.2
RefSeq Size	7500 bp
RefSeq ORF	3870 bp
Locus ID	29645
MW	141.9 kDa
Gene Summary	protein tyrosine phosphatase expressed in smooth muscle cells; may be involved in cell adhesion or cell-cell signaling [RGD, Feb 2006]

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