Postn (NM_001108550) Rat Tagged ORF Clone

CAT＃: RR204952

TrueORF^®

Postn (Myc-DDK-tagged ORF) - Rat periostin, osteoblast specific factor (Postn), (10 ug)

Datasheet

SDS

Plasmid Map

"NM_001108550" in other vectors (3)

Need custom modification / cloning service?
Get a free quote

CNY 10,360.00

货期*

5周

规格

Cited in 1 publication.

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Plf
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204952 representing NM_001108550 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCCTCTCCTGCCCTTATCTGCTCTGCTGCTGCTGTTCCTGTGTGACGTTGACCCCGCAAATGCCA ACAGTTACTATGACAAGGTCCTAGCTCACAGCCGCATCAGGGGTCGGGATCAGGGCCCAAATGTCTGTGC CCTCCAGCAGATTCTGGGCACCAAAAAGAAATACTTCAGCTCCTGTAAGAACTGGTATCAAGGTGCTATC TGCGGGAAGAAAACCACTGTGCTATATGAATGCTGCCCCGGCTATATGAGAATGGAAGGGATGAAAGGCT GCCCAGCAGTGATGCCCATTGACCATGTTTATGGCACGCTGGGCATCGTGGGAGCCACGACCACTCAACA CTATTCTGATGTCTCGAAGCTCAGGGAAGAGATTGAAGGAAAAGGGTCCTACACATACTTCGCGCCGAGT AACGAAGCTTGGGACAACCTGGATTCCGACATCCGCAGAGGACTAGAGAACAATGTCAATGTTGAGTTAC TGAACGCTTTACACAGCCACATGGTTAATAAGAGAATGCTAACCAAGGACCTGAAACACGGCATGGTTAT TCCTTCAATGTACAACAATCTGGGGCTTTTTATCAATCATTATCCCAATGGGGTTGTCACTGTGAACTGT GCTCGAGTAATCCACGGGAACCAGATTGCCACAAATGGTGTTGTCCATGTCATCGACCGTGTCCTGACAC AAATTGGCACCTCCATCCAAGACTTCATTGAAGCAGAAGATGAGCTTTCATCATTCAGAGCGGCTGCCAT CACTTCTGACCTTTTGGAGTCCCTTGGAAGAGACGGTCACTTCACACTCTTTGCTCCCACCAATGAGGCT TTCGAGAAACTCCCACGAGGAGTCCTAGAAAGGATCATGGGAGACAAAGTGGCTTCTGAAGCTCTCATGA AGTACCACATCCTGAATACCCTCCAGTGCTCTGAGGCTATCACAGGAGGAGCGGTGTTTGAGACCATGGA AGGAAACACTATTGAAATAGGGTGTGAGGGAGACAGCATCTCCATTAACGGAATCAAGATGGTGAACAAG AAAGACATTGTGACGAAGAATGGTGTCATCCACCTGATTGATGAAGTCCTCATTCCTGATTCTGCTAAAC AAGTTATTGAGCTGGCTGGAAAACAGCAAACCACTTTCACGGACCTGGTAGCCCAGTTAGGGTTGGCGTC TTCTCTGAAGCCGGATGGAGAGTACACGCTGTTAGCGCCTGTGAACAATGCGTTCTCTGATGACACTCTG AGCATGGACCAGCGCCTTCTTAAGCTAATTCTGCAAAATCACATATTGAAAGTAAAAGTCGGCCTTAGTG ATCTCTACAATGGACAGATTCTGGAGACCATTGGAGGCAAACAACTCCGTGTCTTCGTGTATCGGACGGC TATCTGCATAGAAAACTCATGCATGGTGAGAGGAAGCAAGCAGGGGAGGAACGGTGCCATTCACATATTC CGAGAGATCATCCAACCGGCGGAGAAGTCCCTGCACGAAAAACTGCGCCAAGATAAGCGCTTCAGCATCT TCCTCAGCCTCCTCGAAGCTGCAGATCTGAAAGATCTTCTGACACAGCCCGGAGATTGGACCTTGTTTGC ACCAACCAATGATGCCTTCAAGGGAATGACTAATGAAGAAAGGGAGATTCTGATTGGGGATAAAAATGCT CTCCAAAACATCATTCTTTACCACCTGACCCCAGGGGTTTATATTGGAAAGGGATTTGAACCCGGAGTCA CCAACATCCTGAAGACCACACAGGGAAGCAAAATCTATGTGAAAGGAGTCAATGAGACGCTTTTGGTGAA TGAGTTGAAGTCCAAAGAATCTGACATCATGACAACAAACGGCGTCATTCACGTTGTGGACAAACTCCTC TATCCAGCAGACATTCCGGTTGGAAATGATCAGCTCTTGGAATTACTGAACAAACTGATAAAATACATCC AAATTAAGTTCGTTCGTGGCAGCACCTTCAAAGAAATCCCCATGACTGTCTATACAACTAAAATTATAAC CAAACTCGTGGAACCAAAAATTAAAGTCATTCAAGGCAGTCTTCAGCCTATTATCAAAACAGAAGGACCT GCAATGACGAAGATCCACATTGAAGGCGAGCCTGACTTCAGGCTGATTAAAGAAGGTGAAACAGTGACAG AAGTGATCCACGGAGAACCAGTCATTAAAAAGTACACCAAAATCATAGACGGGGTTCCTGTTGAAATAAC TGAAAAAGAGACCCGGGAAGAACGCATCATCACAGGTCCTGAGATAAAATACACTAGGATTTCCACAGGA GGTGGGGAAACAGAAGAGACCCTGCAGAAATTCTTGCAAAAAGACACACCTGCAAAGAAGATACAAGCCA ACAAAAGGGTTCAAGGGTCTAGAAGGCGATCAAGAGAAGGCCGTTCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204952 representing NM_001108550 Red=Cloning site Green=Tags(s) MVPLLPLSALLLLFLCDVDPANANSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAI CGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSYTYFAPS NEAWDNLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNC ARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDELSSFRAAAITSDLLESLGRDGHFTLFAPTNEA FEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNK KDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTL SMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIF REIIQPAEKSLHEKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTNEEREILIGDKNA LQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYVKGVNETLLVNELKSKESDIMTTNGVIHVVDKLL YPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYTTKIITKLVEPKIKVIQGSLQPIIKTEGP AMTKIHIEGEPDFRLIKEGETVTEVIHGEPVIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTG GGETEETLQKFLQKDTPAKKIQANKRVQGSRRRSREGRSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001108550
ORF Size	2430 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108550.1, NP_001102020.1
RefSeq Size	3177 bp
RefSeq ORF	2433 bp
Locus ID	361945
MW	90.1 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Ageing related periostin expression increase from cardiac fibroblasts promotes cardiomyocytes senescent. ,null, Biochemical and biophysical research communications ,PubMed ID 25173938 [Postn]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RN204952	Postn (untagged ORF) - Rat periostin, osteoblast specific factor (Postn), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 10,360.00
RR204952L3	Lenti ORF clone of Postn (Myc-DDK-tagged ORF) - Rat periostin, osteoblast specific factor (Postn), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,260.00
RR204952L4	Lenti ORF clone of Postn (mGFP-tagged ORF) - Rat periostin, osteoblast specific factor (Postn), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 13,400.00