Tle3 (NM_053400) Rat Tagged ORF Clone
CAT#: RR204731
- TrueORF®
Tle3 (Myc-DDK-tagged ORF) - Rat transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (Tle3), (10 ug)
"NM_053400" in other vectors (3)
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CNY 9,880.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Esp3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204731 representing NM_053400
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATCCGCAAGGCAGACATCCGGCACCCCATCAACCCGGGCAGCCAGGATTTAAATTCACTGTGGCCG AGTCCTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA CGACAAGCTGGCGAACGAGAAGACAGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTACGGC TTGAATATCGAAATGCACAAGCAGACAGAGATCGCGAAGAGACTGAACACGATTCTAGCCCAGATCATGC CTTTTTTGTCACAGGAGCATCAGCAGCAAGTGGCGCAGGCTGTGGAACGCGCCAAGCAGGTCACCATGAC GGAGCTGAACGCCATCATCGGGCAGCAGCAACTCCAGGCACAGCACCTCTCCCATGCCACACATGGTCCC CCGGTCCAGCTGCCACCCCACCCGTCAGGCCTCCAACCTCCTGGGATTCCCCCAGTGACAGGAAGCAGCT CTGGGTTGCTGGCACTTGGTGCCCTGGGAAGCCAAGCCCACTTGGCAGTGAAGGATGAGAAGAACCATCA TGAACTGGATCACAGAGAGAGAGAGTCCAGCACGAACAATTCTGTGTCACCCTCAGAAAGCCTCCGGGCC AGTGAGAAGCACCGGGGCTCTGCAGACTACAGCATGGAAGCCAAGAAGCGGAAAGCGGAAGAGAAAGACA GCCTGAGCCGATACGACAGCGATGGGGACAAGAGTGATGACCTGGTAGTGGATGTCTCCAATGAGGACCC AGCGACACCCCGGGTGAGCCCAGCACACTCCCCTCCTGAAAATGGGCTGGACAAAGCCCGAGGTCTGAAG AAAGACGCCCCCACCAGCCCAGCCTCTGTGGCTTCCTCCAGCAGCACGCCTTCCTCCAAGACCAAAGACC TTGGTCATAATGACAAATCTTCCACACCTGGGCTCAAGTCCAACACACCAACGCCAAGGAACGATGCCCC AACTCCAGGCACCAGCACCACCCCGGGGCTCCGGTCAATGCCGGGCAAACCTCCAGGCATGGACCCGATA GCCTCGGCCCTGCGCACACCCATCTCCCTCACCAGCTCCTATGCAGCACCCTTTGCCATGATGAGCCACC ACGAGATGAATGGCTCCCTCACCAGCCCAAGCGCCTATGCTGGCCTACACAACATCCCATCCCAGATGAG CGCCGCCGCAGCAGCTGCAGCCGCCGCCTATGGCCGATCGCCAATGGTTGGTTTTGACCCTCATCCCCCG ATGCGGGCCACAGGCCTGCCCTCCAGTCTTGCCTCCATTCCTGGAGGGAAACCGGCGTACTCCTTCCATG TGAGTGCCGACGGGCAGATGCAACCTGTGCCCTTCCCCCATGATGCGCTAGCAGGCCCCGGCATTCCCAG GCATGCCCGGCAGATCAACACACTCAGCCATGGGGAGGTGGTATGTGCCGTGACCATCAGCAACCCCACG AGGCACGTCTACACAGGCGGCAAGGGCTGTGTGAAGATATGGGACATCAGCCAGCCGGGCAGCAAGAGTC CCATCTCCCAGCTGGACTGCCTGAACAGGGACAACTACATCCGCTCATGCAAGCTTCTCCCTGATGGGCG CACGCTCATTGTGGGTGGTGAGGCCAGCACACTCACCATCTGGGACCTGGCCTCGCCCACGCCCCGCATC AAGGCTGAGCTGACGTCCTCGGCTCCAGCCTGTTACGCCCTGGCCATCAGTCCTGATGCCAAAGTCTGTT TCTCCTGCTGCAGTGATGGGAACATTGCAGTTTGGGATCTGCACAACCAGACCCTGGTCAGGCAGTTCCA GGGCCACACGGATGGGGCCAGCTGTATAGACATCTCTCATGATGGCACTAAACTGTGGACCGGGGGCCTG GACAACACTGTGCGCTCCTGGGACCTGCGTGAAGGGCGGCAGCTACAGCAACACGACTTCACCTCCCAGA TCTTCTCCCTGGGTTACTGCCCCACTGGGGAGTGGCTCGCCGTGGGCATGGAGAGCAGCAACGTAGAGGT TCTGCACCACACCAAGCCGGACAAGTACCAACTGCATCTGCACGAGAGCTGCGTGCTGTCCCTCAAGTTT GCCTACTGTGGCAAGTGGTTTGTGAGCACTGGGAAAGACAACCTTCTCAATGCCTGGAGGACGCCTTACG GAGCCAGCATCTTCCAGTCTAAAGAATCCTCGTCCGTCTTGAGCTGTGATATTTCAGCGGATGACAAATA TATTGTAACGGGCTCTGGTGACAAGAAGGCCACAGTTTACGAGGTCATCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204731 representing NM_053400
Red=Cloning site Green=Tags(s) MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGP PVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLAVKDEKNHHELDHRERESSTNNSVSPSESLRA SEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARGLK KDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI ASALRTPISLTSSYAAPFAMMSHHEMNGSLTSPSAYAGLHNIPSQMSAAAAAAAAAYGRSPMVGFDPHPP MRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEVVCAVTISNPT RHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASPTPRI KAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGL DNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKF AYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053400 |
ORF Size | 2292 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053400.1, NP_445852.1 |
RefSeq Size | 4776 bp |
RefSeq ORF | 2295 bp |
Locus ID | 84424 |
UniProt ID | Q9JIT3 |
MW | 82.6 kDa |
Gene Summary | may be involved in transcription regulation in the CNS both during development and in the adult [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204731 | Tle3 (untagged ORF) - Rat transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (Tle3), (10 ug) |
CNY 9,880.00 |
|
RR204731L3 | Lenti ORF clone of Tle3 (Myc-DDK-tagged ORF) - Rat transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (Tle3), (10 ug) |
CNY 11,690.00 |
|
RR204731L4 | Lenti ORF clone of Tle3 (mGFP-tagged ORF) - Rat transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (Tle3), (10 ug) |
CNY 12,830.00 |