Adamts12 (NM_001106420) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204380 representing NM_001106420 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATGTGCTCAGGGGAACTGGATGGCAAAGCTCTCCATGGTGGCCCAGCTTCTTAACTTTGGGGCTT TTTGCCATGGGAGACAAGCTCAGCCCTGGCCGGTTCGCTTCCCAGACCCGAAGCAAGAACATTTTATCAA GTCCCTGCCAGAATACCACATAGTGAGTCCTGTCCAAGTAGATGCCAGTGGCCACTTTCTGTCCTATGGC CTGCATCACCCTGTCACTGGCAGCAGGAAGAAGAGAGCTGCAGGTGGTTCTGGGGACCAGGTGTACTACA GGATTTCACACGAAGAAAAAAACCTGTTTTTCAATCTGACAGTCAACTGGGAATTTCTTTCCAATGGCTA TGTCGTGGAGAGGAGATATGGGAACCTTTCCCATGTGAAGATGGCGGCTTCCTCAGGGCAGCCCTGCCAT CTCAGGGGCACAGTTCTGCAACAGGGCCCCACAATCAGGATGGGGACAGCAGCTCTTAGTGCCTGCCAAG GACTGACTGGATTTTTCCATCTACCACATGGAGACTTTTTCATTGAGCCTGTTAAAAAGCATCCACTGAC CGAGGAAGGTTACCAGCCTCATGTCATATACAGGAGGCAGAGCTACAGAGTTCCAGAGACAAAGGAGCCC ACCTGCGGATTAAAGGACAGCCTTGATAACTCTGTGAAGCAAGAGCTCCAGCGGGAGAAGTGGGAGAGGA AGAACTGGCCAAGCAGAAGCCTCTCCCGACGCTCCATAAGCAAGGAGAGATGGGTGGAGACCCTCGTGGT AGCTGACACGAAGATGGTAGAGTACCATGGAAGTGAGAACGTGGAGTCGTACATCCTCACTATCATGAAT ATGGTCACTGGGTTGTTTCATAACCCAAGCATTGGAAACGCAGTGCACATTGTTGTGGTTCGGCTCATTC TACTTGAAGAAGAGGAGCAAGGATTGAAAATAGTTCACCATGCAGAGAAGACACTGTCCAGCTTCTGCAA GTGGCAGAAGAGCATCAATCCCAAGAGTGACCTCAACCCTGTCCATCATGATGTGGCTGTCCTTATCACC AGAAAGGACATCTGTGCTGGTGTCAATCGCCCTTGTGAGACTCTGGGGCTGTCCCAGCTGTCAGGAATGT GCCAGCCTCACAGGAGCTGTAACATCAACGAAGACTCCGGTCTCCCCCTGGCTTTCACCATTGCGCATGA ACTTGGGCACAGCTTCGGCATCCAACATGATGGGAAGGAAAATGACTGTGAGCCCGTGGGCAGACACCCA TACATCATGTCCCAACAAATTCAGTATGACCCAACTCCACTGACATGGTCGAAATGCAGCAAGGAGTACA TCACTCGCTTCTTGGACCGAGGCAGGGGGTTCTGTCTTGATGACGTCCCCAGAAAGAAAGGCTTAAAGTC CAATGTCATTGCCCCTGGAGTGATTTATGATGTTCATCACCAGTGCCAGCTCCAATACGGCCCAAATGCT ACATTCTGCCAGGAAGTGGAAAATGTTTGCCAGACACTGTGGTGCTCAGTGAAAGGCTTCTGTCGCTCTA AGCTGGATGCTGCTGCAGATGGGACACGTTGTGGTGAGAAGAAGTGGTGTATGGCTGGAAAGTGTATCAC AGTGGGGAAGAAACCAGAGAGCATCCCTGGAGGCTGGGGACGCTGGTCACCCTGGTCACACTGTTCGAGG ACATGTGGGGCAGGAGCTCAGAGCGCAGAGAGGCTCTGCAACAACCCAGAACCAAAGTTTGGAGGGAAAT ACTGCACCGGAGAAAGAAAACGCTATCGCTTATGCAATGTCCACCCCTGCCGCTCAGACACTCCAACTTT CCGACAGATGCAGTGCAGTGAATTTGACACAGTCCCCTACAAAAACCAATTCTACCGCTGGTTCCCAGTG TTTAATCCAGCACATCCTTGTGAGCTGTACTGCAGACCCATAGATGAACAGTTTTCTGAGAGAATGCTGG AAGCCGTCATTGATGGCACCCCGTGCTTTGAAGGTGGCAACAGCAGAAATGTCTGTATTAATGGCATATG TAAGAGGGTTGGCTGTGACTATGAGATTGATTCCAATGCTACAGAGGATCGCTGTGGCGTGTGCCTTGGA GATGGCTCTGCCTGCCAGACTGTGAAGAAGGTGTTTAGACAGAAGGAAGGATCCGGTTACATTGACATCG GACTTATTCCCAAAGGTGCAAGGGATATAAGAGTGATGGAAATCAAGGCAGCCGGCAATTTCCTGGCCAT TAGGAGTGAAGATCCAGAAAAATATTACCTCAACGGAGGATTTATCATCCAGTGGAATGGAAACTATAAA CTGGCAGGGACCGTCTTCCAGTATGACAGGAAAGGAGATCTGGAGAGACTTATGGCTCCAGGCCCCACCA ACGAGTCAGTGTGGCTCCAGCTACTATTCCAGGTGACTAACCCCGGTATCAAGTATGAGTACACAGTCCG GAAAGATGGCCTTGACAATGATGTGGAGAAGTTGCTGTACTTCTGGCAATTTGGCCGCTGGACAGAGTGC AGTGTAACATGTGGAACAGGTATCCGCCGGCAGACTGCTCATTGTGTCAAGAAGGGCCATGGGATAGTGA AAACTACCTTCTGCAACCCAGAAACACAGCCCAGCGTGAGACAGAAAAAGTGCTATGAAAAGGACTGTCC ACCCAGGTGGTGGGCCGGAGAGTGGGAAGCATGTTCAATGACATGTGGGCCGTACGGAGAAAAAAAGAGA ACAGTGCTGTGCATCCAGACCATGGGCTCTGATGAGCAGGCTCTCCCAGCTACAGACTGCCAGCACCTGC TGAAGCCCAAGACACTGGTTTCCTGCAACAGAGACATTCTGTGTCCATCAGATTGGACAGTAGGCAACTG GAGTGAGTGCTCTGTTTCCTGTGGTGGTGGAGTGCGGATTCGCAGTGTCACATGTGCCAAGAACCTTAAT GAACCCTGTGACAAGACACGGAAACCCAACAGTCGAGCTCTGTGTGGCCTCCAGCAGTGCCCATTCAGCC GTAGAGTTCTGAAACCCAATAAAGATACAGTTCCCAGTGGGAAAAATCCAACAACATCAGAACATGACCA CTTCAAGCCCATCCCAGCATCTACATCCAGGCCCACGCCACTGTCCACACCCACAGTGCCTGAGTCTGTG AGCACAAGTACCCCCACAATCAACAGCCTTGGCTCCACCATAACATCCCAAGAAGAACCAGATGGGATAG GATGGCAGAATAATTCAACCCAAGCTGAAGAGGACTCCCACATCCCTACTTCTGTTGGAAGCACTTCCCA GACTCCCCTCACTTCCTGGTCTTGGAGTATGCAGCCTGATGATGAAAACGTTTCCAGTTCAGCTATAGGT CCAACTTCAGAGAGTGACTTTTGGGCAACCACAAGTGATTCTGGCTTATCATCTAGCAATGCTATGACTT GGCAGGTAACTCCATTTTATAGCACTGCGACCACAGAACCGGAAGTGGAGATACATAGTGGCTCAGGGGA AGACAGTGACCAGCCTTTGAACAAGGAGGAAAACAACTCTGTGCTATGGAACAAGATCAGAGTACCTGAA CGTGATGCTCCCATGGAGATGGATGCAGAAATACCACTTGGACCTCCACCAACATCTTATGTGACAGAAG AGTCCTCATGGCCTCCCTTCAGTACAATGATGAAAAGCTCACTACCTGCCTGGTCCTTCAAAAATGAGAC ACCCAGGGATGAGGGGATGATCACTGAAAAATCAGGGAATATTCCACTCCCTCTTGGAGGAGACCACCAG ACAACATCCCCAGAGAAGTTAGGAAACAATGACCAACTGGCATCAGCAAATAGTACAAATCCAACCCAAG GCTCTGGGCCTGTCCTGACTGAGGAAGATGCCTCCACTCTGATTGAGGAGGGATTTTTGCTAAATGCTTC CAATTACAAGCATCTCATGAAGGACCACAGCCCTGCACACTGGATTGTTGGAAATTGGAGTAAATGCTCT ACCACCTGTGGGCTGGGGGCCTACTGGAGGAGTGTGGAGTGTAGCACCGGGATGAACGCTGACTGCGCCG CCATTCAGAGGCCAGACCCTGCTAAGAAGTGCCACCTGCGCCCCTGTGCTGGCTGGCGAGTGGGAAACTG GAGCAAGTGCTCTCGAAACTGCAGTGGAGGTTTCAAGATTCGTGAGGTTCAGTGCATGGACGGCGTGGAC CATCACCGGAGCCTGAGGCCATTTCACTGTCAGTTCCTGGCCGGCGTTCCTCCTCCGCTGAGTATGAGCT GTAATCTGGAGCCTTGCGAAGAGTGGAAAGTGGAACCCTGGAGCCAGTGTTCTAGGTCCTGTGGAGGCGG TGTTCAGGAGAGAGGAGTGTTTTGCCCAGGAGGTCTCTGCGACTGGACAAAAAGACCTGCATCCACTGTG CCCTGCAACAGACACCTTTGCTGTCACTGGGCCACTGGGAACTGGGAACTGTGCACCACTTCCTGTGGTG GTGGCTCTCAGAAGAGGACCGTTCATTGCATCCCTTCAGAAAACAGTACAACTGAAGATCAAGACCAGTG TTTCTGTGACCACCAAGCCAGACCCCCAGAATTCCAAAACTGCAACCAGCAAGCCTGCCGGAAGAGTGCT GATTTAACTTGCACGAAGGACAGACTTTCAACCAGCTTCTGCCAGACACTAAAATCCATGAAGAAGTGCT CGGTGCCATCAGTACGAGTCCAGTGCTGTCTCTCGTGTCCACAGACACAGAGCATCCACACCCAGAGGCA AAGAAAACAGCAGATGCTCCAAAATCATGACACGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204380 representing NM_001106420 Red=Cloning site Green=Tags(s) MPCAQGNWMAKLSMVAQLLNFGAFCHGRQAQPWPVRFPDPKQEHFIKSLPEYHIVSPVQVDASGHFLSYG LHHPVTGSRKKRAAGGSGDQVYYRISHEEKNLFFNLTVNWEFLSNGYVVERRYGNLSHVKMAASSGQPCH LRGTVLQQGPTIRMGTAALSACQGLTGFFHLPHGDFFIEPVKKHPLTEEGYQPHVIYRRQSYRVPETKEP TCGLKDSLDNSVKQELQREKWERKNWPSRSLSRRSISKERWVETLVVADTKMVEYHGSENVESYILTIMN MVTGLFHNPSIGNAVHIVVVRLILLEEEEQGLKIVHHAEKTLSSFCKWQKSINPKSDLNPVHHDVAVLIT RKDICAGVNRPCETLGLSQLSGMCQPHRSCNINEDSGLPLAFTIAHELGHSFGIQHDGKENDCEPVGRHP YIMSQQIQYDPTPLTWSKCSKEYITRFLDRGRGFCLDDVPRKKGLKSNVIAPGVIYDVHHQCQLQYGPNA TFCQEVENVCQTLWCSVKGFCRSKLDAAADGTRCGEKKWCMAGKCITVGKKPESIPGGWGRWSPWSHCSR TCGAGAQSAERLCNNPEPKFGGKYCTGERKRYRLCNVHPCRSDTPTFRQMQCSEFDTVPYKNQFYRWFPV FNPAHPCELYCRPIDEQFSERMLEAVIDGTPCFEGGNSRNVCINGICKRVGCDYEIDSNATEDRCGVCLG DGSACQTVKKVFRQKEGSGYIDIGLIPKGARDIRVMEIKAAGNFLAIRSEDPEKYYLNGGFIIQWNGNYK LAGTVFQYDRKGDLERLMAPGPTNESVWLQLLFQVTNPGIKYEYTVRKDGLDNDVEKLLYFWQFGRWTEC SVTCGTGIRRQTAHCVKKGHGIVKTTFCNPETQPSVRQKKCYEKDCPPRWWAGEWEACSMTCGPYGEKKR TVLCIQTMGSDEQALPATDCQHLLKPKTLVSCNRDILCPSDWTVGNWSECSVSCGGGVRIRSVTCAKNLN EPCDKTRKPNSRALCGLQQCPFSRRVLKPNKDTVPSGKNPTTSEHDHFKPIPASTSRPTPLSTPTVPESV STSTPTINSLGSTITSQEEPDGIGWQNNSTQAEEDSHIPTSVGSTSQTPLTSWSWSMQPDDENVSSSAIG PTSESDFWATTSDSGLSSSNAMTWQVTPFYSTATTEPEVEIHSGSGEDSDQPLNKEENNSVLWNKIRVPE RDAPMEMDAEIPLGPPPTSYVTEESSWPPFSTMMKSSLPAWSFKNETPRDEGMITEKSGNIPLPLGGDHQ TTSPEKLGNNDQLASANSTNPTQGSGPVLTEEDASTLIEEGFLLNASNYKHLMKDHSPAHWIVGNWSKCS TTCGLGAYWRSVECSTGMNADCAAIQRPDPAKKCHLRPCAGWRVGNWSKCSRNCSGGFKIREVQCMDGVD HHRSLRPFHCQFLAGVPPPLSMSCNLEPCEEWKVEPWSQCSRSCGGGVQERGVFCPGGLCDWTKRPASTV PCNRHLCCHWATGNWELCTTSCGGGSQKRTVHCIPSENSTTEDQDQCFCDHQARPPEFQNCNQQACRKSA DLTCTKDRLSTSFCQTLKSMKKCSVPSVRVQCCLSCPQTQSIHTQRQRKQQMLQNHDTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106420
ORF Size	4797 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106420.3, NP_001099890.2
RefSeq Size	8571 bp
RefSeq ORF	4800 bp
Locus ID	294809
MW	178.5 kDa

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