Adamts12 (NM_001106420) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204380 representing NM_001106420 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATGTGCTCAGGGGAACTGGATGGCAAAGCTCTCCATGGTGGCCCAGCTTCTTAACTTTGGGGCTT TTTGCCATGGGAGACAAGCTCAGCCCTGGCCGGTTCGCTTCCCAGACCCGAAGCAAGAACATTTTATCAA GTCCCTGCCAGAATACCACATAGTGAGTCCTGTCCAAGTAGATGCCAGTGGCCACTTTCTGTCCTATGGC CTGCATCACCCTGTCACTGGCAGCAGGAAGAAGAGAGCTGCAGGTGGTTCTGGGGACCAGGTGTACTACA GGATTTCACACGAAGAAAAAAACCTGTTTTTCAATCTGACAGTCAACTGGGAATTTCTTTCCAATGGCTA TGTCGTGGAGAGGAGATATGGGAACCTTTCCCATGTGAAGATGGCGGCTTCCTCAGGGCAGCCCTGCCAT CTCAGGGGCACAGTTCTGCAACAGGGCCCCACAATCAGGATGGGGACAGCAGCTCTTAGTGCCTGCCAAG GACTGACTGGATTTTTCCATCTACCACATGGAGACTTTTTCATTGAGCCTGTTAAAAAGCATCCACTGAC CGAGGAAGGTTACCAGCCTCATGTCATATACAGGAGGCAGAGCTACAGAGTTCCAGAGACAAAGGAGCCC ACCTGCGGATTAAAGGACAGCCTTGATAACTCTGTGAAGCAAGAGCTCCAGCGGGAGAAGTGGGAGAGGA AGAACTGGCCAAGCAGAAGCCTCTCCCGACGCTCCATAAGCAAGGAGAGATGGGTGGAGACCCTCGTGGT AGCTGACACGAAGATGGTAGAGTACCATGGAAGTGAGAACGTGGAGTCGTACATCCTCACTATCATGAAT ATGGTCACTGGGTTGTTTCATAACCCAAGCATTGGAAACGCAGTGCACATTGTTGTGGTTCGGCTCATTC TACTTGAAGAAGAGGAGCAAGGATTGAAAATAGTTCACCATGCAGAGAAGACACTGTCCAGCTTCTGCAA GTGGCAGAAGAGCATCAATCCCAAGAGTGACCTCAACCCTGTCCATCATGATGTGGCTGTCCTTATCACC AGAAAGGACATCTGTGCTGGTGTCAATCGCCCTTGTGAGACTCTGGGGCTGTCCCAGCTGTCAGGAATGT GCCAGCCTCACAGGAGCTGTAACATCAACGAAGACTCCGGTCTCCCCCTGGCTTTCACCATTGCGCATGA ACTTGGGCACAGCTTCGGCATCCAACATGATGGGAAGGAAAATGACTGTGAGCCCGTGGGCAGACACCCA TACATCATGTCCCAACAAATTCAGTATGACCCAACTCCACTGACATGGTCGAAATGCAGCAAGGAGTACA TCACTCGCTTCTTGGACCGAGGCAGGGGGTTCTGTCTTGATGACGTCCCCAGAAAGAAAGGCTTAAAGTC CAATGTCATTGCCCCTGGAGTGATTTATGATGTTCATCACCAGTGCCAGCTCCAATACGGCCCAAATGCT ACATTCTGCCAGGAAGTGGAAAATGTTTGCCAGACACTGTGGTGCTCAGTGAAAGGCTTCTGTCGCTCTA AGCTGGATGCTGCTGCAGATGGGACACGTTGTGGTGAGAAGAAGTGGTGTATGGCTGGAAAGTGTATCAC AGTGGGGAAGAAACCAGAGAGCATCCCTGGAGGCTGGGGACGCTGGTCACCCTGGTCACACTGTTCGAGG ACATGTGGGGCAGGAGCTCAGAGCGCAGAGAGGCTCTGCAACAACCCAGAACCAAAGTTTGGAGGGAAAT ACTGCACCGGAGAAAGAAAACGCTATCGCTTATGCAATGTCCACCCCTGCCGCTCAGACACTCCAACTTT CCGACAGATGCAGTGCAGTGAATTTGACACAGTCCCCTACAAAAACCAATTCTACCGCTGGTTCCCAGTG TTTAATCCAGCACATCCTTGTGAGCTGTACTGCAGACCCATAGATGAACAGTTTTCTGAGAGAATGCTGG AAGCCGTCATTGATGGCACCCCGTGCTTTGAAGGTGGCAACAGCAGAAATGTCTGTATTAATGGCATATG TAAGAGGGTTGGCTGTGACTATGAGATTGATTCCAATGCTACAGAGGATCGCTGTGGCGTGTGCCTTGGA GATGGCTCTGCCTGCCAGACTGTGAAGAAGGTGTTTAGACAGAAGGAAGGATCCGGTTACATTGACATCG GACTTATTCCCAAAGGTGCAAGGGATATAAGAGTGATGGAAATCAAGGCAGCCGGCAATTTCCTGGCCAT TAGGAGTGAAGATCCAGAAAAATATTACCTCAACGGAGGATTTATCATCCAGTGGAATGGAAACTATAAA CTGGCAGGGACCGTCTTCCAGTATGACAGGAAAGGAGATCTGGAGAGACTTATGGCTCCAGGCCCCACCA ACGAGTCAGTGTGGCTCCAGCTACTATTCCAGGTGACTAACCCCGGTATCAAGTATGAGTACACAGTCCG GAAAGATGGCCTTGACAATGATGTGGAGAAGTTGCTGTACTTCTGGCAATTTGGCCGCTGGACAGAGTGC AGTGTAACATGTGGAACAGGTATCCGCCGGCAGACTGCTCATTGTGTCAAGAAGGGCCATGGGATAGTGA AAACTACCTTCTGCAACCCAGAAACACAGCCCAGCGTGAGACAGAAAAAGTGCTATGAAAAGGACTGTCC ACCCAGGTGGTGGGCCGGAGAGTGGGAAGCATGTTCAATGACATGTGGGCCGTACGGAGAAAAAAAGAGA ACAGTGCTGTGCATCCAGACCATGGGCTCTGATGAGCAGGCTCTCCCAGCTACAGACTGCCAGCACCTGC TGAAGCCCAAGACACTGGTTTCCTGCAACAGAGACATTCTGTGTCCATCAGATTGGACAGTAGGCAACTG GAGTGAGTGCTCTGTTTCCTGTGGTGGTGGAGTGCGGATTCGCAGTGTCACATGTGCCAAGAACCTTAAT GAACCCTGTGACAAGACACGGAAACCCAACAGTCGAGCTCTGTGTGGCCTCCAGCAGTGCCCATTCAGCC GTAGAGTTCTGAAACCCAATAAAGATACAGTTCCCAGTGGGAAAAATCCAACAACATCAGAACATGACCA CTTCAAGCCCATCCCAGCATCTACATCCAGGCCCACGCCACTGTCCACACCCACAGTGCCTGAGTCTGTG AGCACAAGTACCCCCACAATCAACAGCCTTGGCTCCACCATAACATCCCAAGAAGAACCAGATGGGATAG GATGGCAGAATAATTCAACCCAAGCTGAAGAGGACTCCCACATCCCTACTTCTGTTGGAAGCACTTCCCA GACTCCCCTCACTTCCTGGTCTTGGAGTATGCAGCCTGATGATGAAAACGTTTCCAGTTCAGCTATAGGT CCAACTTCAGAGAGTGACTTTTGGGCAACCACAAGTGATTCTGGCTTATCATCTAGCAATGCTATGACTT GGCAGGTAACTCCATTTTATAGCACTGCGACCACAGAACCGGAAGTGGAGATACATAGTGGCTCAGGGGA AGACAGTGACCAGCCTTTGAACAAGGAGGAAAACAACTCTGTGCTATGGAACAAGATCAGAGTACCTGAA CGTGATGCTCCCATGGAGATGGATGCAGAAATACCACTTGGACCTCCACCAACATCTTATGTGACAGAAG AGTCCTCATGGCCTCCCTTCAGTACAATGATGAAAAGCTCACTACCTGCCTGGTCCTTCAAAAATGAGAC ACCCAGGGATGAGGGGATGATCACTGAAAAATCAGGGAATATTCCACTCCCTCTTGGAGGAGACCACCAG ACAACATCCCCAGAGAAGTTAGGAAACAATGACCAACTGGCATCAGCAAATAGTACAAATCCAACCCAAG GCTCTGGGCCTGTCCTGACTGAGGAAGATGCCTCCACTCTGATTGAGGAGGGATTTTTGCTAAATGCTTC CAATTACAAGCATCTCATGAAGGACCACAGCCCTGCACACTGGATTGTTGGAAATTGGAGTAAATGCTCT ACCACCTGTGGGCTGGGGGCCTACTGGAGGAGTGTGGAGTGTAGCACCGGGATGAACGCTGACTGCGCCG CCATTCAGAGGCCAGACCCTGCTAAGAAGTGCCACCTGCGCCCCTGTGCTGGCTGGCGAGTGGGAAACTG GAGCAAGTGCTCTCGAAACTGCAGTGGAGGTTTCAAGATTCGTGAGGTTCAGTGCATGGACGGCGTGGAC CATCACCGGAGCCTGAGGCCATTTCACTGTCAGTTCCTGGCCGGCGTTCCTCCTCCGCTGAGTATGAGCT GTAATCTGGAGCCTTGCGAAGAGTGGAAAGTGGAACCCTGGAGCCAGTGTTCTAGGTCCTGTGGAGGCGG TGTTCAGGAGAGAGGAGTGTTTTGCCCAGGAGGTCTCTGCGACTGGACAAAAAGACCTGCATCCACTGTG CCCTGCAACAGACACCTTTGCTGTCACTGGGCCACTGGGAACTGGGAACTGTGCACCACTTCCTGTGGTG GTGGCTCTCAGAAGAGGACCGTTCATTGCATCCCTTCAGAAAACAGTACAACTGAAGATCAAGACCAGTG TTTCTGTGACCACCAAGCCAGACCCCCAGAATTCCAAAACTGCAACCAGCAAGCCTGCCGGAAGAGTGCT GATTTAACTTGCACGAAGGACAGACTTTCAACCAGCTTCTGCCAGACACTAAAATCCATGAAGAAGTGCT CGGTGCCATCAGTACGAGTCCAGTGCTGTCTCTCGTGTCCACAGACACAGAGCATCCACACCCAGAGGCA AAGAAAACAGCAGATGCTCCAAAATCATGACACGCTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106420
Insert Size	4800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106420.3, NP_001099890.2
RefSeq Size	8571 bp
RefSeq ORF	4800 bp
Locus ID	294809

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