Eri1 (NM_001014143) Rat Tagged ORF Clone
CAT#: RR204120
- TrueORF®
Eri1 (Myc-DDK-tagged ORF) - Rat three prime histone mRNA exonuclease 1 (Thex1), (10 ug)
"NM_001014143" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RGD1308378; Thex1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204120 representing NM_001014143
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGAGCGGGGCCGGGAGCATGGCGGCGACGCGGCGCAGCAGAAGACGCCGCGGCCAGAGTGTG AGGAGTCCCGGCCGCTGAGCGTGGAGAAAAAGCAGCGATGCAGACTTGATGGCAAAGACACAGATGGATC TAAGTTCATAACCTCCAATGGCGGTGACTTCAGTGACCCGGTTTACAAAGAGATTGCAATGACAAATGGT TGTATCAACAGGATGAGCAAGGAGGAGCTCAGAGCTAAACTTTCAGAATTCAAGCTTGAAACAAGAGGAG TCAAGGATGTTCTAAAGAAGAGACTGAAAAACTATTACAAGAAGCAGAAGCTGATGCTGAAAGAAAGCAA CGCCGTGGACAGTTACTATGACTATATTTGTATTATTGACTTTGAGGCCACCTGTGAAGAGGGGAACCCA GCCGAGTTCCTACATGAAATTATTGAGTTTCCTGTTGTTCTGTTGAATACACACAGCTTAGAGATCGAAG ACACGTTTCAGCAGTATGTGAGACCAGAGGTGAATTCTCAGCTTTCAGAGTTCTGCATTGGTCTTACTGG AATTACTCAGGATCAGGTAGACAGAGCCGATGCTTTCCCTCAGGTCCTGAAAAAAGTAATCGAGTGGATG AAGTCAAAGGAGTTGGGAACTAAGTACAAATACTGCATATTAACAGATGGTTCCTGGGATATGAGTAAGT TCTTAAACATCCAGTGCCAACTAAGCAGACTCAAGTACCCTTCTTTTGCTAAAAAGTGGATCAATATTCG CAAGTCTTACGGAAACTTTTATAAGGTTCCAAGAAGCCAGACCAAACTAACAATAATGCTTGAAAAATTA GGCATGGATTATGATGGGCGACCTCACAGTGGTCTTGATGACTCTAAGAACATTGCTAGAATAGCGGTTC GGATGCTTCAGGATGGATGTGAACTTCGAATCAATGAAAAACTGCACGGAGGACAGCTAATGAGTGTGTC CTCATCCTTACCAGTAGAGGGCGCTCCAGCCCCACAGATGCCGCATAGTAGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204120 representing NM_001014143
Red=Cloning site Green=Tags(s) MEDERGREHGGDAAQQKTPRPECEESRPLSVEKKQRCRLDGKDTDGSKFITSNGGDFSDPVYKEIAMTNG CINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESNAVDSYYDYICIIDFEATCEEGNP AEFLHEIIEFPVVLLNTHSLEIEDTFQQYVRPEVNSQLSEFCIGLTGITQDQVDRADAFPQVLKKVIEWM KSKELGTKYKYCILTDGSWDMSKFLNIQCQLSRLKYPSFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKL GMDYDGRPHSGLDDSKNIARIAVRMLQDGCELRINEKLHGGQLMSVSSSLPVEGAPAPQMPHSRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001014143 |
ORF Size | 1035 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001014143.1, NP_001014165.1 |
RefSeq Size | 2896 bp |
RefSeq ORF | 1038 bp |
Locus ID | 361159 |
UniProt ID | Q5FVR4 |
MW | 39.6 kDa |
Gene Summary | RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Binds with high affinity to the 3' side of the stem-loop structure and to the downstream cleavage product (DCP) of histone pre-mRNAs. Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204120 | Eri1 (untagged ORF) - Rat three prime histone mRNA exonuclease 1 (Thex1), (10 ug) |
CNY 3,990.00 |
|
RR204120L3 | Lenti ORF clone of Eri1 (Myc-DDK-tagged ORF) - Rat three prime histone mRNA exonuclease 1 (Thex1), (10 ug) |
CNY 6,080.00 |
|
RR204120L4 | Lenti ORF clone of Eri1 (mGFP-tagged ORF) - Rat three prime histone mRNA exonuclease 1 (Thex1), (10 ug) |
CNY 6,650.00 |