Usp54 (NM_001008863) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204003 representing NM_001008863 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTGGAAAAGAAATTACTTTTCTGGAGGCCGAGGTAGCGTTCAAGGAATGTTTGCACCTCGGAGCT CAATGTCTATTGCCCCCAGCAAAGGCCTCAGCAATGAGCCAGGGCAAAACAGCTGCTTCCTCAATAGCGC GCTACAGGTTTTGTGGCACTTGGACATCTTCCGCCGCAGCTTTCGGCAGCTCACATCTCACAAGTGCATG GGCGATTCCTGCATCTTCTGTGCGCTCAAGGGCATCTTTAAGCAGTTCCAGTGCAGTAGTGAAAAAGTGC TTCCTTCTGACACTCTCCGTAGTGCTCTGGCGAAGACTTTCCAGGATGAACAGCGTTTCCAGCTGGGGAT TATGGATGACGCTGCAGAATGCTTTGAAAACCTCCTGATGAGAATTCACTTCCATATCGCGGACGAAACC AAAGAGGATATATGTACTGCTCCACACTGCATTTCCCACCAGAAGTTTGCGATGACGTTGTTTGAGCAGT GTGTGTGCACGAGCTGCGGTGCCACCTCTGACCCACTGCCCTTCATCCAGATGGTGCACTACATCTCCAC CACTTCCCTTTGTAACCAGGCCATTTGTATGCTGGAAAAGCGGGAGAAACCTTCACCGGGTATGTTTGGT GAGCTGCTTCAGAATGCCAGCACCATGGGGGACCTGCGGGACTGTCCGAGCAACTGTGGGGAGAGAATTC GGATCCGCCGTGTGTTGATGAATGCACCACAGATTATCACGATTGGGCTGGTTTGGGACTCAGACCATTC CGACTTGGCAGAGGATGTCATCCACAGTCTGGGCACCTGCCTTAAGTTGGGTGATCTGTTTTTCAGAGTG ACAGATGACCGAGCCAAGCAGTCGGAGCTGTACTTAGTAGGAATGATCTGTTACTATGGCAAGCATTATT CTACATTCTTCTTTCAAACCAAGATCCGAAAATGGATGTATTTTGATGATGCTCATGTCAAGGAGATTGG TCCGAAGTGGAAGGACGTGGTGACCAAATGCATTAAAGGGCACTATCAGCCCTTGCTGCTGCTCTATGCA GATCCCCAGGGTACCCCCGTGTCTGCCCAGGACCTGCCTCCCCACGCTCAGTTGCTGTCACACACCAAGA CATGCTACGACAGTGAAGACTCAGGACACCTGACTGACAGTGAATGTAATCAGAAACACACAACGAAGAA AGGGCCCCTGGTGGAGCGCAGGAGAGGCTCTGGCCGCATTCGGAGGAAAGCTGATGCCCCACAGGCCTCA GGCTATCACAGTGAAGGAGAAACACTGAAAGAGAAGCAGGCTCCTAGGAATGCCTCCAAATCCAGCAGCA CCAGCAGGCTAAAGGATTTCAAAGAGACAGTCAGCAATATGATCCACAGCAGGCCTTCCTTGGCTTCTCA GACCAATGCAGCATCTCCCTGTGTAGGCAGGGCAGGAGACCAGCCAGACAAGATACCAGCAAGGAACTTA CCTCTACACTCTCGGGGCTGGGAAACGGAGAGTACCAGCAGTGAGGCAAGATCCAGTTCTTCCAGCAAAT ACCGCCCAACATGGAGACCTAAACGAGAATCCCTAAATATTGACAGCATTTTCAGTAAGGACAAAAGGAA GCATTGTGGTTACACCCAGCTTAAGACCTTTCCTGAAGATGCAGCTAAAGAATTTGCACAAGATGAAGTA AGCAATCCAGTTGCTAACGATATTAAAGATGGTGGACCAAGCTCACAGCATAAACTCTGGGGAACAGCCA GGCCAGGCTCTCATCTTTTAGAACAGCATCCTCGACTAATCCAGCGGATGGAATCTGGCTATGAGAGCAG CGAGAGGAACAGCAGCAGCCCTGTCAGTTTGGATGCTGCCCCGCCTGAATGCTTGAATGTCTACAGGGAT CAAAGCACAAAGAGAGCTGTTGGGTTTGTTCCTTCTTGGCGTCATATCCCAAAGTCTCACAGCAGTAGCA TCCTGGAAGTGGACTCCACAGCACCCGTGACTGGCTGGACAGAGACCCAACCTTTCTCAGATGGGGAGAT CACCTCTAAAAGTGAGTTGGATGAACTTCAGGAAGAAGTGGCCAGGCGGGCCCAGGAACAGGAACTTCGA AAGAAACGGGAGAAGGAATTAGAGGCTGCTAAAGGCTTTAATCCCCATCCTAGCCGATACATGGACTTGG ATGAACTACAGAATCAGGGGAGGAGTGACGGCTTTGAAAGGTCCTTGCAAGAGGCAAATTCAGTATTTGA AGAGTCGCTGCATCTGGAGCAAGAGGGAGACTGTGCTGCCGCTCTGGCTCTCTGTAACGAAGCTATCTCT AAACTAAGACTTACCCTGCATGATGCCAGCTCTAGCACGCACAGCAGAGCCCTAGTCGATAAGAAGTTGC AAATCAGTATTCGAAAAGCTCGAAGCCTGCAAGATCGCATGCAGCAGCAGCCACCATCGCAGCAGCCGGT GCAGCCCTCAGCCTCTCTCCCATCACAGGGGGGCGGCCTCCCTCAGCCGACCAGTGAACAGTCTGTCCCG CTCCAAGTACTGCTAAGCCAGCAGACACAGCTGGAACCCTGCAAGGATTCAGAGCTCGGCGCCACTTCCC CTTTCTTCCACTCACCTGCTTCCTGCCCTGAGCCACACTCATCCCTCGTCTCACCGTCACCTGCTCAGTC TGTCTCTCAGCACAGTCCTCCTGGGACGTCTGCCTTGAAGCTCCTGAAGTCTTTTGAGGTGGATATCGTC AACCACTCTGCATTTCACAGGCAGGATTTACCTAAAGCAACAGGTAGAACTGAGATGAATTCTCAACATG AATGCCTGCCCTTTGATGCCCTTGAAGATAGGCTCCAAGGTCACAGGGAGGACAACAGCTGTTGTAGCAA ATTCCCACCCCAAGAAGGAAGAGACACAACTCAAGATCAGCTGTTGGAAGGAAGGAAGACTCCTGTTGAC ATATCCATGGGGATGCCTTGGTCACATTCAGCAGGAGGAGCTACTTCTGAGAGAGTTATGCATAGCCTAA ACAGTCCTTCAAGTCCATCAGCTCAGCCTGCTGTTCCCCCCTACGGTGCCTGCCACCCTATAATGTCTGC TGCTGCTTCACCTGTGCTTCATGCTGCTGACCCCATGCAGAAACTTAACCAACATCTCCAAGCCCAAAGT CTCCAAACTTCATTGGCTTCAAAAGTGGTCAGAGGCAGTGAGGAGCCCTATAGGCCAGAGTTCCCCAGTA CAAAAGGCCTTGTCCGCTCATTGGCAGAGCAGTTCCAGAAGATTCGGAATACTTCCACGAGAGATGTTAT AGGTTCCCAGGATCGAAGTTTGCCAAATGGTCTAAGGAAGAGCTCTTCTCCTTCTGACTTTATGCTTCCA TTATCCCAGGGTCCAGAAAAAGAACACTGTCGTTGGGTAAACCAGCCCCCTTCTCCAGATGGCAGGGAAA GACAGCCCTGCTGGGAAGAACCTGCAGGGCATCCTTCTGTTTCCATTGACTCTGGGCTTCCTAATGGTGA AGCCTCGAGGAGAAGACAGCCCAGACTGGCCGAGGCAGACATATACCAAGGAAAGCTACCCCAAGTGACA GATACTAGGCCCACGGAGCTGGGCAGTAGTGTCAGCTTGGGGACTTCTTTGCCTTTGGATTCTTGGGTAA ATGTCACAAGGCTCTGTGATTCTCAAGTTAAACACAGGGCTCCTGGGCTAGGAGTCAAGTCCTCCTCCCA TGATTCCCGTACATGTGTAACCTATCCAGAAAGAAATCCTATCCTTTTGCATCCACACTGGAACCAAGAC ACAGAGCAGGAGACGTCTGAACTGGAGTCTCTCTACCAAGCCAGTCTCCAGGCCTCCTCTCATACTGGCT ATTCTGATTGGAGGTCACAGGATGTGGCCTGGCAGCCACTTAGCCTAACAGGTTCTGCAGATGGCATGGG GAGGAGACTGCACTCAGCCCCTGGTCTTGATCTTTCAAAGACTCCAACAGCAGAAATGGAGCGCATTCTT TATGAACCGAGTACAGTGCCTGTCTCTCAGGATTCATCAAACGTGAGGAAAAAGACTTTGGAAACCGGGC ACCATTGTTCCAGCTCCTCTTCCCTCCCTGTCATCCATGATCCTCCTGTATTTCTCCTTGATCCTAAACT CTACCCTCCCCAACCACAGTTCCTGTCCCCAGATGTCCTGATGCCCAGCATGGCAGGGGAGCCCTATAGA TCCCCAGGAACTTCGAGGAGTGTCCAACAGTTTCTGGCTATGTGTGACAGGGATGAAACTCCCCAAGGGG TCAAGTACACAGGAAGGACTTTGAACTACCGAAGTCTACCTCATCGTTCCAGAACTGATGCTTCCTGGGG ACCAGGGACAGAAACCAACCAGCACATTGGGGCCAGAGTCTTAACTGCGCCAGCATGCAAGCCTCAGCTA ACCTACACTGCCACACTACCAGAAAGACACCAGGGCCTTCAGGTTCCTCATGCTCAGTCCTGGGGCAATC TTTTCCACTTACCCTCCCATCCTTCTGCTGTTCACCCAGGGTCCCCACCATCCAGCAGTCTTCATGTACT CCATAGGTCATCTTGGAATTCGGGTTCTGTTCTGGGGTCCCGAACACCTGGTCCTCGACGAATAGACGTG CCCCCAGATGATGACGGGAGACAGAGCCAATACCCCTCTCAGTATAGACACAGGAGTGCAGGGGAGGAGA GAGTTAGGTTTGCTCTGTCAAACACTGCGGGCACAGAGCAGAGCAGGGTGAGACTGCTACAGCACAGCAG GTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204003 representing NM_001008863 Red=Cloning site Green=Tags(s) MSWKRNYFSGGRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTSHKCM GDSCIFCALKGIFKQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADET KEDICTAPHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLEKREKPSPGMFG ELLQNASTMGDLRDCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRV TDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYA DPQGTPVSAQDLPPHAQLLSHTKTCYDSEDSGHLTDSECNQKHTTKKGPLVERRRGSGRIRRKADAPQAS GYHSEGETLKEKQAPRNASKSSSTSRLKDFKETVSNMIHSRPSLASQTNAASPCVGRAGDQPDKIPARNL PLHSRGWETESTSSEARSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLKTFPEDAAKEFAQDEV SNPVANDIKDGGPSSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPECLNVYRD QSTKRAVGFVPSWRHIPKSHSSSILEVDSTAPVTGWTETQPFSDGEITSKSELDELQEEVARRAQEQELR KKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSVFEESLHLEQEGDCAAALALCNEAIS KLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQPPSQQPVQPSASLPSQGGGLPQPTSEQSVP LQVLLSQQTQLEPCKDSELGATSPFFHSPASCPEPHSSLVSPSPAQSVSQHSPPGTSALKLLKSFEVDIV NHSAFHRQDLPKATGRTEMNSQHECLPFDALEDRLQGHREDNSCCSKFPPQEGRDTTQDQLLEGRKTPVD ISMGMPWSHSAGGATSERVMHSLNSPSSPSAQPAVPPYGACHPIMSAAASPVLHAADPMQKLNQHLQAQS LQTSLASKVVRGSEEPYRPEFPSTKGLVRSLAEQFQKIRNTSTRDVIGSQDRSLPNGLRKSSSPSDFMLP LSQGPEKEHCRWVNQPPSPDGRERQPCWEEPAGHPSVSIDSGLPNGEASRRRQPRLAEADIYQGKLPQVT DTRPTELGSSVSLGTSLPLDSWVNVTRLCDSQVKHRAPGLGVKSSSHDSRTCVTYPERNPILLHPHWNQD TEQETSELESLYQASLQASSHTGYSDWRSQDVAWQPLSLTGSADGMGRRLHSAPGLDLSKTPTAEMERIL YEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYR SPGTSRSVQQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGTETNQHIGARVLTAPACKPQL TYTATLPERHQGLQVPHAQSWGNLFHLPSHPSAVHPGSPPSSSLHVLHRSSWNSGSVLGSRTPGPRRIDV PPDDDGRQSQYPSQYRHRSAGEERVRFALSNTAGTEQSRVRLLQHSRW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001008863
ORF Size	4764 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001008863.3, NP_001008863.2
RefSeq Size	5738 bp
RefSeq ORF	4767 bp
Locus ID	408223
UniProt ID	Q6IE24
MW	176.2 kDa
Gene Summary	Has no peptidase activity.[UniProtKB/Swiss-Prot Function]

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