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Itgam (NM_012711) Rat Tagged ORF Clone
CAT＃: RR203904
- TrueORF®
Itgam (Myc-DDK-tagged ORF) - Rat integrin alpha M (Itgam), (10 ug)
"NM_012711" in other vectors (3)
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CNY 18,530.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cd11b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203904 representing NM_012711
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCTTAAAGTTCTTCTGGCGACAGTCCTAACCTTGTGTCATGGCTTCAATTTGGACACTGAAAATC CCATGACCTTCCAAGAGAATGCAAGTGGCTTCGGGCAGAGTGTGATCCAGCTTGGTGAAACCCGAGTGGT TGTTGCAGCCCCCCAGGAGGTAAAGGCGGTTAACCAGACAGGTGCCCTCTACCAGTGTGACTACAGCACA AACCGGTGCGACCCCATTCCCCTGCAAGTACCTCCAGAGGCTGTGAATATGTCCTTGGGCCTGTCCCTGG CTGCTACTACCGTCCCTCCACAGCTGCTGGCTTGTGGCCCCACAGTACACCAGAACTGCAAGGAGAACAC TTACGTCAATGGATTGTGCTACCTATTCGGCTCCAACCTGCTGAGGAAGCCCCAGCAGTTCCCAGAGGCT CTCAGAGGATGTCCTCAGCAGGAGAGCAACATTGCCTTCTTGATTGACGGCTCCGGTAGCATCAACACGA TCGACTTTCAGAAGATGAAGGAATTTGTCTCAACTGTGATGGACCAGTTCCAAAAGTCTAAAACCTTGTT CTCTTTGATGCAGTACTCTGATGAATTCCGGACTCACTTCACCTTCAATGATTTCAAGAGAAACCCTGAC CCAAAATCACATGTGAGACCCATAAGGCAGCTGAATGGAAGGACAAAAACTGCCTCAGGGATCCGTAAAG TAGTGAGAGAACTGTTTCAGAAAATCAATGGGGCCCGGGACAATGCCGCGAAGATCCTAGTTGTCATCAC AGACGGAGAAAAGTTTGGTGACCCCCTAAATTATGAGGATGTCATTCCTGAGGCAGAGGAAGCAGGGATC ATTCGCTACGTTATTGGGGTGGGAAACGCCTTCCACAAACCACAGTCCCGCAGAGAGCTTGACACCATCG CGTCTAAGCCAGCTGGTGATCACGTGTTCCAAGTGGACAACTTTGAAGCTCTGAATACCATTCGGAACCA GCTCCAGGAAAAGATCTTTGCAATTGAGGGCACCCAGACAGGAAGTACCAGCTCCTTTGAGCATGAGATG TCTCAAGAAGGCTTTAGCGCTGCCATTACCTCCAACGGTCCCTTGCTGGGCTCCGTGGGGAGCTTTGACT GGGCAGGTGGAGCCTTCCTGTATCCATCAAAGGATAAAGCCAGTTTCATCAACACAACCAGAATAGATTC AGACATGAACGATGCTTACTTGGGTTATGCTTCTGCAGTCATCTCGAGGAACCGCGTCCAAAGCTTGGTT TTAGGGGCACCTCGATATCAGCATATCGGCCTGGTGGTGATGTTCAAGCAGAATTTCGGTGCCTGGGAGC CCCACACTGATATCAAGGGCAGCCAGATCGGCTCTTATTTTGGGGCCTCCCTCTGTTCTGTGGACATGGA CGCTGATGGCAATACCAACTTGATCCTCATTGGGGCCCCTCATCACTATGAGAAGACCCGAGGGGGCCAG GTGTCAGTGTGCCCCTTGCCTAGGGGGAGAGCACGGTGGCAGTGTGAAGCTATTCTCCATGGCGATCAGG GCCATCCATGGGGTCGCTTTGGGGCAGCATTGACAGTGCTGGGAGATGTGAATGGAGACAAACTGACAGA TGTGGCCATTGGGGCCCCAGGAGAGCAGGAGAATCAGGGTGCTGTCTACATTTTTCATGGAGCATCAGTA GCCAGCATCAGTACCCCTCACAGCCAGCGGATCGCAGGCGCCCGCTTCTCCCCCGGGCTCCAGTACTTTG GGCAGTCTCTGAGTGGGGGCAAGGATCTCACAATGGACGGCCTGATGGACTTGGCTGTGGGGGCGCAGGG GCGTCTTCTGCTGATCAGAGCCCAGCCTGTGCTGAGACTGGAGGCAACCATGGAATTCAGCCCCAAGAAT CTAGCAAGGAGTGTGTTTGCGTGTCAAGAAGAAGTAGTCAAAAACAAGGATGCTGGGGAGGTCAGAGTCT GCCTCCAAGTCCGCAAGAACACCAAGGACAGGCTGCGAGAAGGAGACATCCAGAGCACTGTCACTTATGA CCTGGCTTTAGACCCCGGCCGCTCAGTTGTCCGAGCCTTCTTTGATGAAACAAAGAACGGCATACTCAGG CGCATCCGGGTCTTTGGATTGACACAGAAATGTGAAACCCTGAAGCTAATTTTACCGGACTGTGTGGACA ACTCAGTGAGTCCCATCATCCTGCGCCTTAATTACACACTGGTTGGGGAGCCCTTGCGGTCCTCTAGGGA CCTTCGGCCAGTTCTGGCTATGGAGGCTCAGAGGATCTTCACAGCTATGTTTCCCTTTGAGAAGAATTGT GGCAATGACACCATTTGCCAGGATGATCTCAGCATCACCGTGAGTTCCACACGCGTGAACACTTTGGTGG TGGGAGACTCCCGGGACTTTGACGTGAGTGTGACTCTGAGAAATGATGGCGAGGATTCCTATGGGACCAA GGTTACCTGCTATTACCCATCTGGCTTGTCTTACCGGAAGGTGTCAGCAAGCCAGAACCAGTTCTCCAAG AAGCCTTGGCGTGTGATAGCTGAGCCCAGTTCTTCTGAAGGGCAAGGGGTTCTGAAGAGCACCATCTGGG ACATAAACCATCCCATCTTTCCCGCTAATTCTGAGGTCACGTTTAATGTCACATTTGATGTGGACTCTGA TGCCTCCCTTGGGAACAAACTGCTCCTCAAGGTCGTTGTGACCAGTGAGAACAACGTGTCCAGGACCGAC AAAACTGAGTCTCAGTTGGAGTTGCCTGTGAAGTACGCCATCTACATGGTCGTCACCAGTGGTGAGAGTT CTATCAAATATCTCAACTTCACAGCTTCAGAGATGACCAGTAAGGTCATACAGCATCAGTACCAGTTCAA CAACCTGGGCCAGAGGAGCTTCCCTGTCAGCGTGGTCTTCTGGATCCCTGTCCAGATCAACAAGGTGACC ATATGGGATCCTCCCCAGGTCACCTTCTCCCAGAACCTCTCAAGTGTCTGTCGCACTGAGCAGAAATCCC CCTCTCACTCCAAGTTCCAGGATGAGCTTGAAAGGACCCCAGTGCTGAACTGCTCTGTTGCAGTCTGCAA GAGAATCCAGTGTGACATCCCTTCCTTCAACAGTAAAGAAATATTCAACGTCACCCTCCAGGGCAATCTG CTATTTGACTGGTACATCGAGACTTCTCATGACCACCTCCTGCTTGTGAGCACTGCCGAGATCCTCTTTA ACGACTCCGCATTTGCCCTACTTCCGGGGCAGGAGACGTTTGTGAAGGCTCAGACAGAGACCAAAGTGGA GCCATATACAGTTCACAATCCTGTACCACTCATTGTGGGCAGCTCCGTCGGAGGCCTGGTGCTCTTGGCT CTCATCACTGCTGGCCTATACAAGCTTGGCTTTTTCAAACGACAGTACAAGGACATGATGAATGAAGCCG GTGGCCAAGATGGCCCACCTCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR203904 representing NM_012711
Red=Cloning site Green=Tags(s) MTLKVLLATVLTLCHGFNLDTENPMTFQENASGFGQSVIQLGETRVVVAAPQEVKAVNQTGALYQCDYST NRCDPIPLQVPPEAVNMSLGLSLAATTVPPQLLACGPTVHQNCKENTYVNGLCYLFGSNLLRKPQQFPEA LRGCPQQESNIAFLIDGSGSINTIDFQKMKEFVSTVMDQFQKSKTLFSLMQYSDEFRTHFTFNDFKRNPD PKSHVRPIRQLNGRTKTASGIRKVVRELFQKINGARDNAAKILVVITDGEKFGDPLNYEDVIPEAEEAGI IRYVIGVGNAFHKPQSRRELDTIASKPAGDHVFQVDNFEALNTIRNQLQEKIFAIEGTQTGSTSSFEHEM SQEGFSAAITSNGPLLGSVGSFDWAGGAFLYPSKDKASFINTTRIDSDMNDAYLGYASAVISRNRVQSLV LGAPRYQHIGLVVMFKQNFGAWEPHTDIKGSQIGSYFGASLCSVDMDADGNTNLILIGAPHHYEKTRGGQ VSVCPLPRGRARWQCEAILHGDQGHPWGRFGAALTVLGDVNGDKLTDVAIGAPGEQENQGAVYIFHGASV ASISTPHSQRIAGARFSPGLQYFGQSLSGGKDLTMDGLMDLAVGAQGRLLLIRAQPVLRLEATMEFSPKN LARSVFACQEEVVKNKDAGEVRVCLQVRKNTKDRLREGDIQSTVTYDLALDPGRSVVRAFFDETKNGILR RIRVFGLTQKCETLKLILPDCVDNSVSPIILRLNYTLVGEPLRSSRDLRPVLAMEAQRIFTAMFPFEKNC GNDTICQDDLSITVSSTRVNTLVVGDSRDFDVSVTLRNDGEDSYGTKVTCYYPSGLSYRKVSASQNQFSK KPWRVIAEPSSSEGQGVLKSTIWDINHPIFPANSEVTFNVTFDVDSDASLGNKLLLKVVVTSENNVSRTD KTESQLELPVKYAIYMVVTSGESSIKYLNFTASEMTSKVIQHQYQFNNLGQRSFPVSVVFWIPVQINKVT IWDPPQVTFSQNLSSVCRTEQKSPSHSKFQDELERTPVLNCSVAVCKRIQCDIPSFNSKEIFNVTLQGNL LFDWYIETSHDHLLLVSTAEILFNDSAFALLPGQETFVKAQTETKVEPYTVHNPVPLIVGSSVGGLVLLA LITAGLYKLGFFKRQYKDMMNEAGGQDGPPQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012711 |
| ORF Size | 3453 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012711.1, NP_036843.1 |
| RefSeq Size | 3456 bp |
| RefSeq ORF | 3456 bp |
| Locus ID | 25021 |
| MW | 126.9 kDa |
| Gene Summary | mouse homolog is an integrin alpha subunit that interacts with integrin beta 2 subunit to form Mac-1; involved in immune response [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203904 | Itgam (untagged ORF) - Rat integrin alpha M (Itgam), (10 ug) |
CNY 8,024.00 |
|
| RR203904L3 | Lenti ORF clone of Itgam (Myc-DDK-tagged ORF) - Rat integrin alpha M (Itgam), (10 ug) |
CNY 20,050.00 |
|
| RR203904L4 | Lenti ORF clone of Itgam (mGFP-tagged ORF) - Rat integrin alpha M (Itgam), (10 ug) |
CNY 22,040.00 |
