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Bag1 (NM_001106647) Rat Tagged ORF Clone
CAT＃: RR203877
- TrueORF®
Bag1 (Myc-DDK-tagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug)
"NM_001106647" in other vectors (3)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Bag-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203877 representing NM_001106647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGCGGATCGCGGCGGCGCGCGGCGACCGCGAGGCGACCAGGAGCCGCTGGGACCCCGGCTGCGCGCCC CTCGGTCGGCCCGGGAGACGCGCCAGTCGGAGTCCCGGGCCGAGCGTGGCTTGCCTCCCTCTCAGCGTTC ATCTGTCCGCAGTGCAGCCAGCGGGCATGACCGATCCACCAGGGGCGCCGCCTCCGGCGCTTGCAAGCCG CGGGTGAAGAAGAAAGTCCGGCCCCGCTCTTCTCAGAGCGAGAAGGTAGCTCACAGCAAGGAGTTGACTA GAAGTAAGAAGTTGACCCGCAGCAAGAAAGTGACCGGGACCCAGGAGGCGACCCAAGTAGAGGAGGTGAC CACGATCGAGGAGGCGACCCAGACCGAGGAGATTACGGTAGCGGAAGAGGTGACCCAGACCGAGAACATG GCCCAGACCGAGGAGATGGTCCAGACGGAGGAAATGGAACCACCCACACTCAGCGTGGTCGTCACCCACA GCAATGAGAGGTATGACCTTCTTGTTACCCCACAGCAAGGTAACAGTGAGCCAATTGTCCAAGACCTGGC TCAGCTTGTTGAAGAGGCCACAGGAGTTCCACTACCTTTTCAGAAGCTCATATTTAAGGGCAAATCTCTG AAAGAAATGGAAACACCCTTGTCAGCACTTGGAATGCAAAATGGTTGCCGAGTCATGTTAATTGGTGAAA AGAGCAATCCAGAAGAAGAGGCTGAGTTGAAAAAGCTGAAGGACTTGGAGGTATCTGTGGAGAAGACAGC TAACCACCTGGAAGAGTTGAATAAAGAGCTTTCTGACATCCAGCAGGGTTTTCTGGCTAAGGAATTACAA GCGGAGGCTCTCTGCAGACTTGATAGGAAAATAAAGGCCACAATTGAGCAATTCATGAAGATCTTGGAGG AGATTGACACAATGGTCCTACCAGAAAACTTTAAAGACAGCAGGCTAAAAAGGAAGAATTTGGTGAAAAA GGTTCAGGTGTTCCTAGCAGAGTGTGATACAGTGGAGCAGTACATCTGCCAAGAGACAGAGCGGCTGCAG TCTACAAACTTGGCCCTGCCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203877 representing NM_001106647
Red=Cloning site Green=Tags(s) LADRGGARRPRGDQEPLGPRLRAPRSARETRQSESRAERGLPPSQRSSVRSAASGHDRSTRGAASGACKP RVKKKVRPRSSQSEKVAHSKELTRSKKLTRSKKVTGTQEATQVEEVTTIEEATQTEEITVAEEVTQTENM AQTEEMVQTEEMEPPTLSVVVTHSNERYDLLVTPQQGNSEPIVQDLAQLVEEATGVPLPFQKLIFKGKSL KEMETPLSALGMQNGCRVMLIGEKSNPEEEAELKKLKDLEVSVEKTANHLEELNKELSDIQQGFLAKELQ AEALCRLDRKIKATIEQFMKILEEIDTMVLPENFKDSRLKRKNLVKKVQVFLAECDTVEQYICQETERLQ STNLALPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106647 |
| ORF Size | 1074 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106647.3, NP_001100117.2 |
| RefSeq Size | 1308 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 297994 |
| UniProt ID | B0K019 |
| MW | 40.1 kDa |
| Gene Summary | The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. Studies in human and mouse suggest that the protein encoded by this gene (referred to as Bcl2-associated athanogene) binds to Bcl2 protein. It enhances the anti-apoptotic effects of Bcl2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least two protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site and an alternative, downstream, AUG translation initiation site. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203877 | Bag1 (untagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug) |
CNY 3,990.00 |
|
| RR203877L3 | Lenti ORF clone of Bag1 (Myc-DDK-tagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug) |
CNY 6,080.00 |
|
| RR203877L4 | Lenti ORF clone of Bag1 (mGFP-tagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug) |
CNY 6,650.00 |
