Atcay (NM_001040190) Rat Tagged ORF Clone

CAT#: RR203203

  • TrueORF®

Atcay (Myc-DDK-tagged ORF) - Rat ataxia, cerebellar, Cayman type (Atcay), (10 ug)



  "NM_001040190" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR203203 representing NM_001040190
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACCACAGAAGCTACATTACGAATGGAAAATGTGGACGTGAGGGATGAATGGCAGGATGAGGATC
TGCCCAGACCGCTCCCAGAAGACACCGGGGAGGATCACCTGGGCGGCACGGTGGAAGACTCCTCCTCTCC
TCCCTCCACCCTAAACCTGAGTGGAGCACATCGGAAGAGAAAGACGCTGGTGGCCCCAGAGATCAACATC
TCTCTGGACCAAAGTGAGGGCTCTCTGCTGTCTGATGACTTCCTCGACACCCCTGATGACCTGGACATCA
ATGTGGATGACATTGAGACGCCAGATGAAACTGACTCTCTGGAGTTCTTGGGAAATGGCAATGAGCTTGA
GTGGGAAGATGACACCCCAGTGGCCACCGCTAAAAACATGCCTGGTGACAGCGCAGACCTGTTTGGGGAC
GGCTCTGGGGAAGACGGCAGTGCAGCCAATGGCCGTCTGTGGCGGACTGTCATCATCGGGGAGCAGGAGC
ATCGCATTGACCTGCATATGATCCGGCCCTACATGAAGGTGGTGACCCACGGAGGATACTACGGAGAAGG
TCTCAACGCCATCATCGTCTTTGCGGCCTGCTTCCTGCCAGATAGCAGCTCTCCAGACTACCACTACATC
ATGGAGAATCTCTTCCTGTACGTCATCAGCAGCCTAGAGCTGCTAGTGGCTGAGGACTACATGATCGTGT
ACCTGAATGGTGCCACGCCCCGGAGGAGGATGCCGGGCATTGGCTGGCTGAAGAAGTGTTACCACATGAT
TGACAGGAGACTGAGGAAGAATCTAAAGTCCCTGATCATCGTCCACCCCTCCTGGTTCATTCGCACCGTG
CTGGCCATCTCCCGGCCCTTCATCAGTGTCAAGTTCATCAGTAAAATCCAGTATGTGCACAGCCTCGAGG
AGCTGGAGCAGCTCATCCCCATGGAGCACGTGCAGCTGCCAGCCTGTGTCCTGCAATATGAAGAACAGAG
ACTCCGAGCCAAGAGGGAGAGCGCTCGGCCACCGCAGCCCGAGTTCCTCCTGCCCAGGTCTGAAGAAAAA
CCAGAGACTGTGGAAGAAGAGGACAGGGCAGCAGAGGTGACAGAGGACCAGGAAACGAGCATGTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR203203 representing NM_001040190
Red=Cloning site Green=Tags(s)

MGTTEATLRMENVDVRDEWQDEDLPRPLPEDTGEDHLGGTVEDSSSPPSTLNLSGAHRKRKTLVAPEINI
SLDQSEGSLLSDDFLDTPDDLDINVDDIETPDETDSLEFLGNGNELEWEDDTPVATAKNMPGDSADLFGD
GSGEDGSAANGRLWRTVIIGEQEHRIDLHMIRPYMKVVTHGGYYGEGLNAIIVFAACFLPDSSSPDYHYI
MENLFLYVISSLELLVAEDYMIVYLNGATPRRRMPGIGWLKKCYHMIDRRLRKNLKSLIIVHPSWFIRTV
LAISRPFISVKFISKIQYVHSLEELEQLIPMEHVQLPACVLQYEEQRLRAKRESARPPQPEFLLPRSEEK
PETVEEEDRAAEVTEDQETSMS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001040190
ORF Size 1116 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001040190.1, NP_001035280.1
RefSeq Size 2722 bp
RefSeq ORF 1119 bp
Locus ID 362826
UniProt ID Q1M168
MW 42.1 kDa
Gene Summary Functions in the development of neural tissues, particularly the postnatal maturation of the cerebellar cortex. May play a role in neurotransmission through regulation of glutaminase/GLS, an enzyme responsible for the production in neurons of the glutamate neurotransmitter. Alternatively, may regulate the localization of mitochondria within axons and dendrites.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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