Spag5 (NM_001044224) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Deepest
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203082 representing NM_001044224 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGGGTGAAAACACTGAATCTCGGCTTGTCGCCTTCGCCCCAATCGGGGAAACCAGCTATGAGAA CTCCTCTCCGAGAGCTTAAACTACAGCCCGAGGCCCTCGCTGATTCAGGGAAAGGTCCTATGTTCTCCTC GCTGACCCCATACCTGCGCAGGCTGGATCTGAAGGAAAGGCGCAACAGCTCATCTCCAGTGAATTTTGTT AATGCAGAGAACTCATCTCCAGTGGATTTTGTCAATACTGAGAACACATCTCCAGTGGATTTTGACAATA CTGAGAACAACTTCCTTTCAGAACAGTTCAGCCATCCTTCAGAGTACATAGAAGCTTGTCAGCGTGAATC AGATCTAACTCCTGAAAGCAGCTCTCCTTTCAACACACTGGAGAAGGCAGTGGAAGCAATGGATGATTTT GTGGCAGAACCAATAGATGACGGTATAGTTAAGACCATGGTTCTTTTACCCTTTTCCTTAGGGCAGCAGC AAGACCTGATGCTCCAGGCTCACTTAGATACCACAGCAGAGAGAAATAAAAGCTCTCTAAAAGAATCTTT GGGGCTAGAAGACCTGGTGGAAAAGGAGGCGGCACCTTGTGTGGAAGACAGCTTACCAGAAACTGTTGCT ATCAGGCCTGAGCAACCCATCTCTCAGGACCCTCCATTAAGAGACAGTAACACTGAAGCTGCACCTGCGG GCTCAGTTCCTTCTGAAAATGTCTTGGGTTTCACTGTGGCTTGTCTCTCTCCCTCAACTGTCTTGACCAA AGATTTCTCTGTCGATCATGTCGATCCTGGGGAGGAAACTGCAGAGAACAGAGTTATACAGGAAGTGGAA AAGAGTTTTCTCGCACTCCCTGAGGAGGCTGGATTGGGAGATCAGGCACTTGCTGTAAATGCAGAAGACG TCTCATCCTTATACCTGTCATCAAGTCTAGTAGAAATGGGACCCCAGGAGGCTCCAGGCCCAACAGCAGA AGATGCCAGTAGGATTCCTGACTTGGAGTCAGAGACTTGGATGTCCCCATTGGCCTGGCTAGAAAAAGGT GTGAACACGTCAGTCATGCTACAAAGTCTCCGCCAGAGTTTACCCTTTTCAAAATCTGTGCTTCAGGATG CTGCCGTTGGCAATACACCCCACTCCACTTATTCTGTGGGAACTTCCTTTACTCCTCCAGTGCCACTGGA AGTAGGCACCAAGGACAGTACTTCAGAGACAGAGCGCCTCCTCTTGGGCTGCCGTCCTCCAGATCTGGCA GCCTTGTCCCGGCACGACTTGGAAGAGAACCTGTTGAACGCCCTTGTCTTGCTGGAAGTTCTTTCTCACC AGCTGCAAGCCTGGAAGAGTCAGCTGACTGTCCCGAGCCGGCAAGCTCAAGACAGCAGCACACAGACCGA CAGCTCTGCAGCTGTGGTCACTAAGACACCTAAACATCTTCAGGACAGCAAGGAGATTCGACAGGCTCTA CTGCAAGCCAGGAATGTCATACATTCATGGGGGCTAGTCTCTAGAGACCTGATGTCCTTGCTTCACCTGT CCCTGACACATGTGCAAGATGACAGAGTGACAGTGAGTCAGGAGTCTCAGCGTGCAGAGACCCTGGTTTC CTCCTGTTCTCGTGTACTGAAGAAATTGAGGGCAAAGCTCCAGAGCCTCAAAACAGAATGGGAGGAGGCA CGGCACAGGGAGGAAATGGCCCTTAAAGGCAAGGATGCGGCAGAGGCAGTACTGGAGGCTTTCCGTGCAC ACGCTAGCCAGCGCATCAGCCAACTGGAACAGGGTATCACATCTGTGCAGGAATTCAGAGGCCTTCTACA GGAGGCACAGACCCAACTGATAGGGCTTCACACAGAGCAAGAAGAGCTGGCTCAGCACACAGTGAGTCTG ACTTCTGCCTTGCAGCAGGACTGGACATCAGTCCAACAGAATTATGGGACATGGGCAGCTTTGCTGAGTC GGTCTCGAGAACTCACCAAGAAACTCACAGCCAAGAGCCGGCAAGCCCTTCAGGAACGTGATGCTGCAAT TGAGGAAAAGGATCAGGTTGTGAAGGAAGTGGAACAAGTCTCTGCCCATTTAGAGGACTGTAAAGGCCAA ATAGAACAACTGAAGTTGGAAAATAGTCGCCTTACTGCAGATCTCTCGGCTCAGCTGCAGACTCTGGCTA GCACAGAGAACGAACTAAAGGAACTACAGAGTCAGCATTCCCATTGTGTCCAAGACTTGGCCATGAAGGA TGAACTGCTGTGTCAACTTACCCAGAGCAACAAGGAACAGGCTGCTCAGTGGCAAAAGGAAGAAACGGAA CTGAAACACAGACAAGCAGAACTGCTGCAGCAGAAGGCTGTGTTGGCTAAGGAGGTACAGGACCTGAGGG AGACCATGGAGTTTGTAGATGAAGAGAGTCAAGTTGCTCACCTGGAGCTGGGCCAGATCGAGAGTCAGTT GAAAGCCACACTAGAAGTTCTGCGGGAGCGCAGCCTGCAGTGTGAGACCCTCAAGGACACCGTGGAGAGC CTGAGGGCAGAGCTGGCCAGCACCGAAGCAAAGCATGAGCAACAAGCGCTGGAGAAGACGCACCAGCATT CTAAAGAGCTGTGTCTATTGGCGGAGCAGCTACAGAGTCTCACCCTCTTCTTAGAGGCTAAACTAGAGGA GAACAAGGCAGAATCAGACATCATTCTGCCCAGCACAGGCTGTGCTCCAGCCCAGGAACACCCTCCTTCC AGTGATAGCAGCGTCTCAGAACAGATCCCGACAGCAGTGGTAGATGAAGTGCCAGAACCAGCTCCTGTGC CATTGCTTGGAAGTGTTAAGAGTGCTTTCACCCGCGTAGCCTCAACAGCTCCCTTTCGGCCTACAGAGAC CCCAGCCCTGGAGAAGAGCCTGGCAGAAATGAGTGCTGTGTTACAGGAGCTTCAACGCCTCTGTGCCCTG CTGCAAGAATCTAAAGAGGAAGCCGTTGGTGTCCTGCAGAGGGAAATCTGTGAACTGCACACAAGACTAC AGGCCCAAGAAGAAGAACATCAGGAAGCCCAAAAGGCAAAGGAAGCAGACATAGAGAAGTTGAACCAGGC CTTGTGCTTGCGCCACAAGAATGAGAAGGAACTCCTGGAAGTGATACAGAAGCAGAACGAAAAGATCCTG GGGCAGATAGACACGAGTGGCCAGCTCATAAACCTCAGGGAGGAGGTGACCCAGCTTACACGTTTACTTC GGCGGGCAGAGACAGAGACTAAAGTGCTCCAGGAAGCCCTGGAAGGCCAGGTAGATCCCAGCTGTCAGCT GATGGCTACTAACTGGATCCAGGAAAAAGTGTTTCTCTCGCAGGAGGTGAACAAACTGAGGGCTATGTTC CTGGAGGTGAAAAATGAGAAGAAACAGCTGATGGACAAGTATCTGAGCCATAGGCACATCCTGGAGGAGA ATCTTCGGCGCTCTGACACAGAGTTAAAGAAACTTGATGACACAATTCAGCATATCTATGAGACTCTGTT GTCTATCCCAGAGGTAGTGAAGAGTTGCAAGGAGCTACAAGGATTGCTAGAATTTCTGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203082 representing NM_001044224 Red=Cloning site Green=Tags(s) MWRVKTLNLGLSPSPQSGKPAMRTPLRELKLQPEALADSGKGPMFSSLTPYLRRLDLKERRNSSSPVNFV NAENSSPVDFVNTENTSPVDFDNTENNFLSEQFSHPSEYIEACQRESDLTPESSSPFNTLEKAVEAMDDF VAEPIDDGIVKTMVLLPFSLGQQQDLMLQAHLDTTAERNKSSLKESLGLEDLVEKEAAPCVEDSLPETVA IRPEQPISQDPPLRDSNTEAAPAGSVPSENVLGFTVACLSPSTVLTKDFSVDHVDPGEETAENRVIQEVE KSFLALPEEAGLGDQALAVNAEDVSSLYLSSSLVEMGPQEAPGPTAEDASRIPDLESETWMSPLAWLEKG VNTSVMLQSLRQSLPFSKSVLQDAAVGNTPHSTYSVGTSFTPPVPLEVGTKDSTSETERLLLGCRPPDLA ALSRHDLEENLLNALVLLEVLSHQLQAWKSQLTVPSRQAQDSSTQTDSSAAVVTKTPKHLQDSKEIRQAL LQARNVIHSWGLVSRDLMSLLHLSLTHVQDDRVTVSQESQRAETLVSSCSRVLKKLRAKLQSLKTEWEEA RHREEMALKGKDAAEAVLEAFRAHASQRISQLEQGITSVQEFRGLLQEAQTQLIGLHTEQEELAQHTVSL TSALQQDWTSVQQNYGTWAALLSRSRELTKKLTAKSRQALQERDAAIEEKDQVVKEVEQVSAHLEDCKGQ IEQLKLENSRLTADLSAQLQTLASTENELKELQSQHSHCVQDLAMKDELLCQLTQSNKEQAAQWQKEETE LKHRQAELLQQKAVLAKEVQDLRETMEFVDEESQVAHLELGQIESQLKATLEVLRERSLQCETLKDTVES LRAELASTEAKHEQQALEKTHQHSKELCLLAEQLQSLTLFLEAKLEENKAESDIILPSTGCAPAQEHPPS SDSSVSEQIPTAVVDEVPEPAPVPLLGSVKSAFTRVASTAPFRPTETPALEKSLAEMSAVLQELQRLCAL LQESKEEAVGVLQREICELHTRLQAQEEEHQEAQKAKEADIEKLNQALCLRHKNEKELLEVIQKQNEKIL GQIDTSGQLINLREEVTQLTRLLRRAETETKVLQEALEGQVDPSCQLMATNWIQEKVFLSQEVNKLRAMF LEVKNEKKQLMDKYLSHRHILEENLRRSDTELKKLDDTIQHIYETLLSIPEVVKSCKELQGLLEFLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001044224
ORF Size	3561 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001044224.1, NP_001037689.1
RefSeq Size	3881 bp
RefSeq ORF	3564 bp
Locus ID	252918
MW	132.4 kDa
Gene Summary	C-terminal has two leucine zippers which are involved in sperm tail protein interactions [RGD, Feb 2006]

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