Spag5 (NM_001044224) Rat Tagged ORF Clone
CAT#: RR203082
- TrueORF®
Spag5 (Myc-DDK-tagged ORF) - Rat sperm associated antigen 5 (Spag5), (10 ug)
"NM_001044224" in other vectors (1)
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CNY 19,100.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Deepest |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR203082 representing NM_001044224
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGGGTGAAAACACTGAATCTCGGCTTGTCGCCTTCGCCCCAATCGGGGAAACCAGCTATGAGAA CTCCTCTCCGAGAGCTTAAACTACAGCCCGAGGCCCTCGCTGATTCAGGGAAAGGTCCTATGTTCTCCTC GCTGACCCCATACCTGCGCAGGCTGGATCTGAAGGAAAGGCGCAACAGCTCATCTCCAGTGAATTTTGTT AATGCAGAGAACTCATCTCCAGTGGATTTTGTCAATACTGAGAACACATCTCCAGTGGATTTTGACAATA CTGAGAACAACTTCCTTTCAGAACAGTTCAGCCATCCTTCAGAGTACATAGAAGCTTGTCAGCGTGAATC AGATCTAACTCCTGAAAGCAGCTCTCCTTTCAACACACTGGAGAAGGCAGTGGAAGCAATGGATGATTTT GTGGCAGAACCAATAGATGACGGTATAGTTAAGACCATGGTTCTTTTACCCTTTTCCTTAGGGCAGCAGC AAGACCTGATGCTCCAGGCTCACTTAGATACCACAGCAGAGAGAAATAAAAGCTCTCTAAAAGAATCTTT GGGGCTAGAAGACCTGGTGGAAAAGGAGGCGGCACCTTGTGTGGAAGACAGCTTACCAGAAACTGTTGCT ATCAGGCCTGAGCAACCCATCTCTCAGGACCCTCCATTAAGAGACAGTAACACTGAAGCTGCACCTGCGG GCTCAGTTCCTTCTGAAAATGTCTTGGGTTTCACTGTGGCTTGTCTCTCTCCCTCAACTGTCTTGACCAA AGATTTCTCTGTCGATCATGTCGATCCTGGGGAGGAAACTGCAGAGAACAGAGTTATACAGGAAGTGGAA AAGAGTTTTCTCGCACTCCCTGAGGAGGCTGGATTGGGAGATCAGGCACTTGCTGTAAATGCAGAAGACG TCTCATCCTTATACCTGTCATCAAGTCTAGTAGAAATGGGACCCCAGGAGGCTCCAGGCCCAACAGCAGA AGATGCCAGTAGGATTCCTGACTTGGAGTCAGAGACTTGGATGTCCCCATTGGCCTGGCTAGAAAAAGGT GTGAACACGTCAGTCATGCTACAAAGTCTCCGCCAGAGTTTACCCTTTTCAAAATCTGTGCTTCAGGATG CTGCCGTTGGCAATACACCCCACTCCACTTATTCTGTGGGAACTTCCTTTACTCCTCCAGTGCCACTGGA AGTAGGCACCAAGGACAGTACTTCAGAGACAGAGCGCCTCCTCTTGGGCTGCCGTCCTCCAGATCTGGCA GCCTTGTCCCGGCACGACTTGGAAGAGAACCTGTTGAACGCCCTTGTCTTGCTGGAAGTTCTTTCTCACC AGCTGCAAGCCTGGAAGAGTCAGCTGACTGTCCCGAGCCGGCAAGCTCAAGACAGCAGCACACAGACCGA CAGCTCTGCAGCTGTGGTCACTAAGACACCTAAACATCTTCAGGACAGCAAGGAGATTCGACAGGCTCTA CTGCAAGCCAGGAATGTCATACATTCATGGGGGCTAGTCTCTAGAGACCTGATGTCCTTGCTTCACCTGT CCCTGACACATGTGCAAGATGACAGAGTGACAGTGAGTCAGGAGTCTCAGCGTGCAGAGACCCTGGTTTC CTCCTGTTCTCGTGTACTGAAGAAATTGAGGGCAAAGCTCCAGAGCCTCAAAACAGAATGGGAGGAGGCA CGGCACAGGGAGGAAATGGCCCTTAAAGGCAAGGATGCGGCAGAGGCAGTACTGGAGGCTTTCCGTGCAC ACGCTAGCCAGCGCATCAGCCAACTGGAACAGGGTATCACATCTGTGCAGGAATTCAGAGGCCTTCTACA GGAGGCACAGACCCAACTGATAGGGCTTCACACAGAGCAAGAAGAGCTGGCTCAGCACACAGTGAGTCTG ACTTCTGCCTTGCAGCAGGACTGGACATCAGTCCAACAGAATTATGGGACATGGGCAGCTTTGCTGAGTC GGTCTCGAGAACTCACCAAGAAACTCACAGCCAAGAGCCGGCAAGCCCTTCAGGAACGTGATGCTGCAAT TGAGGAAAAGGATCAGGTTGTGAAGGAAGTGGAACAAGTCTCTGCCCATTTAGAGGACTGTAAAGGCCAA ATAGAACAACTGAAGTTGGAAAATAGTCGCCTTACTGCAGATCTCTCGGCTCAGCTGCAGACTCTGGCTA GCACAGAGAACGAACTAAAGGAACTACAGAGTCAGCATTCCCATTGTGTCCAAGACTTGGCCATGAAGGA TGAACTGCTGTGTCAACTTACCCAGAGCAACAAGGAACAGGCTGCTCAGTGGCAAAAGGAAGAAACGGAA CTGAAACACAGACAAGCAGAACTGCTGCAGCAGAAGGCTGTGTTGGCTAAGGAGGTACAGGACCTGAGGG AGACCATGGAGTTTGTAGATGAAGAGAGTCAAGTTGCTCACCTGGAGCTGGGCCAGATCGAGAGTCAGTT GAAAGCCACACTAGAAGTTCTGCGGGAGCGCAGCCTGCAGTGTGAGACCCTCAAGGACACCGTGGAGAGC CTGAGGGCAGAGCTGGCCAGCACCGAAGCAAAGCATGAGCAACAAGCGCTGGAGAAGACGCACCAGCATT CTAAAGAGCTGTGTCTATTGGCGGAGCAGCTACAGAGTCTCACCCTCTTCTTAGAGGCTAAACTAGAGGA GAACAAGGCAGAATCAGACATCATTCTGCCCAGCACAGGCTGTGCTCCAGCCCAGGAACACCCTCCTTCC AGTGATAGCAGCGTCTCAGAACAGATCCCGACAGCAGTGGTAGATGAAGTGCCAGAACCAGCTCCTGTGC CATTGCTTGGAAGTGTTAAGAGTGCTTTCACCCGCGTAGCCTCAACAGCTCCCTTTCGGCCTACAGAGAC CCCAGCCCTGGAGAAGAGCCTGGCAGAAATGAGTGCTGTGTTACAGGAGCTTCAACGCCTCTGTGCCCTG CTGCAAGAATCTAAAGAGGAAGCCGTTGGTGTCCTGCAGAGGGAAATCTGTGAACTGCACACAAGACTAC AGGCCCAAGAAGAAGAACATCAGGAAGCCCAAAAGGCAAAGGAAGCAGACATAGAGAAGTTGAACCAGGC CTTGTGCTTGCGCCACAAGAATGAGAAGGAACTCCTGGAAGTGATACAGAAGCAGAACGAAAAGATCCTG GGGCAGATAGACACGAGTGGCCAGCTCATAAACCTCAGGGAGGAGGTGACCCAGCTTACACGTTTACTTC GGCGGGCAGAGACAGAGACTAAAGTGCTCCAGGAAGCCCTGGAAGGCCAGGTAGATCCCAGCTGTCAGCT GATGGCTACTAACTGGATCCAGGAAAAAGTGTTTCTCTCGCAGGAGGTGAACAAACTGAGGGCTATGTTC CTGGAGGTGAAAAATGAGAAGAAACAGCTGATGGACAAGTATCTGAGCCATAGGCACATCCTGGAGGAGA ATCTTCGGCGCTCTGACACAGAGTTAAAGAAACTTGATGACACAATTCAGCATATCTATGAGACTCTGTT GTCTATCCCAGAGGTAGTGAAGAGTTGCAAGGAGCTACAAGGATTGCTAGAATTTCTGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203082 representing NM_001044224
Red=Cloning site Green=Tags(s) MWRVKTLNLGLSPSPQSGKPAMRTPLRELKLQPEALADSGKGPMFSSLTPYLRRLDLKERRNSSSPVNFV NAENSSPVDFVNTENTSPVDFDNTENNFLSEQFSHPSEYIEACQRESDLTPESSSPFNTLEKAVEAMDDF VAEPIDDGIVKTMVLLPFSLGQQQDLMLQAHLDTTAERNKSSLKESLGLEDLVEKEAAPCVEDSLPETVA IRPEQPISQDPPLRDSNTEAAPAGSVPSENVLGFTVACLSPSTVLTKDFSVDHVDPGEETAENRVIQEVE KSFLALPEEAGLGDQALAVNAEDVSSLYLSSSLVEMGPQEAPGPTAEDASRIPDLESETWMSPLAWLEKG VNTSVMLQSLRQSLPFSKSVLQDAAVGNTPHSTYSVGTSFTPPVPLEVGTKDSTSETERLLLGCRPPDLA ALSRHDLEENLLNALVLLEVLSHQLQAWKSQLTVPSRQAQDSSTQTDSSAAVVTKTPKHLQDSKEIRQAL LQARNVIHSWGLVSRDLMSLLHLSLTHVQDDRVTVSQESQRAETLVSSCSRVLKKLRAKLQSLKTEWEEA RHREEMALKGKDAAEAVLEAFRAHASQRISQLEQGITSVQEFRGLLQEAQTQLIGLHTEQEELAQHTVSL TSALQQDWTSVQQNYGTWAALLSRSRELTKKLTAKSRQALQERDAAIEEKDQVVKEVEQVSAHLEDCKGQ IEQLKLENSRLTADLSAQLQTLASTENELKELQSQHSHCVQDLAMKDELLCQLTQSNKEQAAQWQKEETE LKHRQAELLQQKAVLAKEVQDLRETMEFVDEESQVAHLELGQIESQLKATLEVLRERSLQCETLKDTVES LRAELASTEAKHEQQALEKTHQHSKELCLLAEQLQSLTLFLEAKLEENKAESDIILPSTGCAPAQEHPPS SDSSVSEQIPTAVVDEVPEPAPVPLLGSVKSAFTRVASTAPFRPTETPALEKSLAEMSAVLQELQRLCAL LQESKEEAVGVLQREICELHTRLQAQEEEHQEAQKAKEADIEKLNQALCLRHKNEKELLEVIQKQNEKIL GQIDTSGQLINLREEVTQLTRLLRRAETETKVLQEALEGQVDPSCQLMATNWIQEKVFLSQEVNKLRAMF LEVKNEKKQLMDKYLSHRHILEENLRRSDTELKKLDDTIQHIYETLLSIPEVVKSCKELQGLLEFLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001044224 |
ORF Size | 3561 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001044224.1, NP_001037689.1 |
RefSeq Size | 3881 bp |
RefSeq ORF | 3564 bp |
Locus ID | 252918 |
MW | 132.4 kDa |
Gene Summary | C-terminal has two leucine zippers which are involved in sperm tail protein interactions [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN203082 | Spag5 (untagged ORF) - Rat sperm associated antigen 5 (Spag5), (10 ug) |
CNY 19,100.00 |