Duox1 (NM_153739) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203031 representing NM_153739 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTCTACTCAGCTGTGGCCTGGATCCTCCTTTTTGGGGTGCTGGCTTCTTTGGGAGCTCAGAATC CCGTTTCTTGGGAGGTCCAGCGATTTGATGGATGGTATAACAACCTCATGGAGCACAGATGGGGCAGCAA AGGCTCTCGGCTGCAGCGCCTTGTTCCGGCCAGCTATGCAGATGGTGTGTACCAGCCCTTGAGAGAACCC TACCTGCCTAACCCCCGACACCTTAGCAACAGGGTCATGAGAGGTCCCGCAGGGCAGCCGTCTCTTCGGA ACCGTACAGTCCTGGGTGTCTTCTTCGGCTACCACGTGCTTTCAGACCTGGTGAGTGTGGAAACACCAGG CTGCCCCGCAGAGTTCCTCAACATTTACATCCCACGTGGGGACCCAGTGTTTGACCCTGACAAGCGCGGG AACGTGGTGCTGCCCTTTCAGAGGAGCCGCTGGGACCGCAGCACTGGACAGAGCCCTAGCAACCCCCGAG ACCTGACCAACCAGGTGACTGGCTGGCTAGATGGCAGCGCCATCTATGGCTCCTCTCATTCCTGGAGCGA CACTCTGAGGAGCTTCTCTGGAGGACAGCTGGCTTCTGGCCCTGACCCTGCTTTCCCGAGGAACTCCCAG AACTCTCTGCTCATGTGGATGGCACCAGACCCCGCCACGGGGCAGGGCGGGCCACAGGGGCTGTATGCCT TCGGGGCTCAGCGCGGGAACAGGGAGCCCTTTCTGCAGGCACTGGGCTTGTTGTGGTTTCGATACCACAA CCTGTGTGCCAAGAGGCTGGCGCAGGAGCACCCACACTGGGGGGATGAGGAGCTGTTCCAGCATGCTCGC AAGAGGGTCATTGCCACTTACCAGAACATTGCGATGTATGAGTGGCTGCCCAGCTTCCTGAAGCAAACGC CCCCAGAGTATCCAGGATACCATCCATTTCTGGACCCCAGCATCTCCCCAGAGTTCGTGGTGGCCTCAGA GCAGTTCCTCTCTACCATGGTGCCCCCTGGGGTCTACATGAGGAATGCCAGTTGCCACTTCCAGGGGATT GCCAATCGTAACTCAAGTGTCTCTGGAGCTCTCCGGGTTTGCAACAGCTACTGGAGTCGAGAGAACCCCA AGCTACAAAGGGCTGAAGATGTGGATGCATTGCTGCTGGGCATGGCCTCCCAGATTGCTGAGAGAGAGGA CCACCTAGTCGTTGAGGATGTGCAAGATTTTTGGCCCGGACCACTGAAGTTTTCCCGTACAGACTACCTG GCCAGCTGCCTGCAGAGGGGCCGGGATCTAGGTCTACCTTCATACACCAAAGCCAGGGAGGCACTGGGCC TGCCTCCTGTTTCCCACTGGCAGGACATCAACCCTGCACTCTCGAGGAGCAATGGCACTGTGCTGGAGGC CACGGCTGCCCTGTACAATCAGGATCTGTCCCGGCTGGAGTTACTTGCTGGGGGGCTTCTGGAGAGCCAC GGAGACCCTGGACCCCTTTTCAGTGCTATTGTCCTTGACCAGTTTGTCAGGCTACGAGATGGTGACCGCT ATTGGTTTGAAAACAACAGAAATGGATTGTTCTCTAAGGAAGAGATTGCAGAGATCCGAAACACTTCCCT ACGGGATATTCTAGTGGCTGTCACCAACGTGGACCCCAGTGCCCTACAGCCCAGTGTCTTCTTTTGGCTT GCAGGAGACCCCTGTCCACAGCCAAGCCAGCTCAGCACCCAGGGCCTGCCAGCCTGTGCCCCCCTCTTCG TTCGGGACTATTTCAAGGGGAGTGGATTTGGCTTCGGACTCACCATTGGGACCCTTTGCTGCTTCCCCTT AGTCAGCCTCCTCAGTGCCTGGATTGTTGCCCGGCTTCGGATGAGGAATTTCAAGAGGCTCCAGAGACAA GACCGCCAGAGCATCATGTGCGAGAAGCTTGTAGGGGGAGTGGAAGCTTTGGAGTGGCAGGGCCGCAAGG AACCCTGTAGGCCAGTGCTTGTGCACTTACAGCCTGGACAGATCCGAGTCGTGGATGGCAGGCTCACTGT GCTCCGTACCATCCAGCTGCGGCCCCCACAGCAGGTCAACCTCATCCTGTCCAGCAACCGTGGACGCCGC ACCCTGCTGCTCAAGATCCCCAAGGAGTACGACCTGGTGCTGTTGTTTAACATGGAGGAGGAACGGCAGG CACTGGTGGAAAACATCAGGGCAGCTCTGAAGGAGAATGGACTGAGCTTCCAGGAGTGGGAGCTGAGGGA GCAGGAGCTGATGAGGGCAGCAGTGACCAGGCAACAGCGGGGTCACCTCCTGGAGACTTTTTTCAGGCAC CTTTTCTCCCAGGTGTTAGACATCAATCAAGCAGATGCAGGGACTCTGCCACTGGACTCTTCGACAAAAG TGCGTGAGGCTCTGACCTGCGAGCTGAGCAGAGCTGAGTTTGCGGACTCCCTAGGCCTCAAGCCCCAGGA TATGTTTGTGGAGTCCATGTTTTCTCTGGCCGACAAGGACGGCAATGGCTACCTGTCCTTCCGGGAGTTC CTGGACATCCTGGTGGTCTTCATGAAAGGCTCCCCAGAGGAGAAGTCTCGCCTCATGTTCCGAATGTATG ACTTTGACGGGAATGGTCTCATCTCTAAGGATGAGTTCATTAGGATGCTAAGGTCCTTCATCGAGATCTC CAACAACTGCCTGTCCAAGGACCAGCTGGCTGAGGTGGTGGAGTCCATGTTCCGGGAGTCGGGCTTCCAG GACAAGGAGGAGCTGACCTGGGAGGACTTCCATTTCATGCTGCGCGACCACGACAGCGATCTCCGATTCA CACAGCTCTGTGTCAAAGGGGTGGAAGTGCCGGAAGTGATCAAGAACCTCTGCCGGAGAGCCTCCTACAT CAGCCAGGAGAAGATCTGCCCTTCACCTAGAATGAGCGCCCATTGTGCCCGAAACAATACGAAGACAGCG TCATCCCCACAGAGACTGCAGTGCCCCGTGGACACGGACCCTCCTCAAGAGATTCGACGGAGGTTTGGCA AAAAGGTAACTTCATTCCAGCCCTTACTGTTCACTGAGGCGCACCGAGAGAAGTTTCAACGCAGCCGCCG CCACCAAACCGTGCAGCAGTTCAAGCGCTTCATTGAGAACTACAGGCGCCACATTGGCTGTGTGGCAGTG TTCTACACCATCACTGGGGCGCTCTTCCTGGAGAGAGCCTACTACTATGCGTTTGCCGCGCACCACAGCG GCATCACCGATACTACCCGTGTGGGCATCATCCTGTCCCGAGGCACGGCGGCCAGCATCTCCTTCATGTT TTCCTACATTTTGCTCACTATGTGCCGCAACCTCATCACCTTCCTGCGGGAGACCTTCCTCAACCGCTAC ATTCCCTTTGATGCCGCTGTGGACTTCCACCGCTTCATCGCCTCCACAGCCATCATCCTTACAGTCTTAC ACAGTGCTGGCCACGTAGTGAATGTGTACCTGTTCTCCATCAGCCCCCTCAGTGTCCTCTCTTGCCTCTT CCCTGACCTCTTCCACGATGACGGGTCGGAGTTCCCTCAGAAGTATTACTGGTGGTTCTTCCAGACTGTG CCAGGTCTCACTGGGGTCCTGCTGCTCCTGGCCCTGGCCATCATGTATGTCTTCGCTTCCCACCACTTCC GCCGCCGCAGTTTCCGGGGCTTCTGGCTGACCCACCACCTCTACATTTTTCTCTACATCCTGCTCATCAT CCATGGCAGCTTTGCTCTGATCCAGATGCCCCGATTCCACATCTTCTTCCTGGTCCCCGCCATTATCTAT GTGGGGGACAAGCTGGTGAGCCTGAGCCGGAAGAAGGTGGAGATCAGCGTGGTGAAGGCTGAGCTGCTGC CTTCAGGAGTGACCCACCTGCGGTTCCAGAGGCCCCAGGGCTTTGAGTATAAGTCAGGGCAGTGGGTTCG AATTGCATGCCTGGCTCTGGGAACCACCGAGTACCACCCTTTCACACTGACTTCTGCACCCCACGAGGAC ACACTCAGCCTGCACATCCGGGCAGCTGGACCCTGGACCACCCGCCTCAGGGAGATCTACTCACCCCCAA CGGGTGACACCTGTGCCAGATACCCAAAGCTGTACCTCGATGGACCATTTGGAGAAGGCCACCAGGAGTG GCATAAATTTGAGGTGTCAGTACTAGTGGGAGCTGGAATTGGGGTCACCCCCTTTGCCTCCATCCTCAAA GACCTGGTTTTCAAGTCATCAGTCAGCTGCCAAGTGTTCTGTAAGAAGATCTACTTCATCTGGGTGACAA GGACTCAGCGGCAGTTTGAGTGGCTGGCTGACATCATCCGGGAAGTTGAGGAGAATGACAGCCGGGACCT GGTGTCTGTGCACATCTATATCACTCAGCTGGCTGAGAAGTTTGACCTCAGGACCACCATGCTGTACATC TGTGAGAGGCACTTCCAGAAGGTGCTGAACAGGAGTCTGTTCACGGGCCTGCGCTCTGTCACCCACTTTG GTCGCCCTCCCTTTGAGCCCTTTTTCAACTCTCTGCAGGAGGTCCACCCCCAAGTGCGAAAGATTGGAGT ATTTAGCTGTGGTCCCCCTGGCATGACCAAGAACGTGGAGAAGGCCTGTCAGCTCATCAACAAGCAGGAT AGGACTCACTTCTCTCACCATTATGAGAACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203031 representing NM_153739 Red=Cloning site Green=Tags(s) MAVYSAVAWILLFGVLASLGAQNPVSWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLREP YLPNPRHLSNRVMRGPAGQPSLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIYIPRGDPVFDPDKRG NVVLPFQRSRWDRSTGQSPSNPRDLTNQVTGWLDGSAIYGSSHSWSDTLRSFSGGQLASGPDPAFPRNSQ NSLLMWMAPDPATGQGGPQGLYAFGAQRGNREPFLQALGLLWFRYHNLCAKRLAQEHPHWGDEELFQHAR KRVIATYQNIAMYEWLPSFLKQTPPEYPGYHPFLDPSISPEFVVASEQFLSTMVPPGVYMRNASCHFQGI ANRNSSVSGALRVCNSYWSRENPKLQRAEDVDALLLGMASQIAEREDHLVVEDVQDFWPGPLKFSRTDYL ASCLQRGRDLGLPSYTKAREALGLPPVSHWQDINPALSRSNGTVLEATAALYNQDLSRLELLAGGLLESH GDPGPLFSAIVLDQFVRLRDGDRYWFENNRNGLFSKEEIAEIRNTSLRDILVAVTNVDPSALQPSVFFWL AGDPCPQPSQLSTQGLPACAPLFVRDYFKGSGFGFGLTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQRQ DRQSIMCEKLVGGVEALEWQGRKEPCRPVLVHLQPGQIRVVDGRLTVLRTIQLRPPQQVNLILSSNRGRR TLLLKIPKEYDLVLLFNMEEERQALVENIRAALKENGLSFQEWELREQELMRAAVTRQQRGHLLETFFRH LFSQVLDINQADAGTLPLDSSTKVREALTCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYLSFREF LDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKDQLAEVVESMFRESGFQ DKEELTWEDFHFMLRDHDSDLRFTQLCVKGVEVPEVIKNLCRRASYISQEKICPSPRMSAHCARNNTKTA SSPQRLQCPVDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSRRHQTVQQFKRFIENYRRHIGCVAV FYTITGALFLERAYYYAFAAHHSGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRY IPFDAAVDFHRFIASTAIILTVLHSAGHVVNVYLFSISPLSVLSCLFPDLFHDDGSEFPQKYYWWFFQTV PGLTGVLLLLALAIMYVFASHHFRRRSFRGFWLTHHLYIFLYILLIIHGSFALIQMPRFHIFFLVPAIIY VGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHED TLSLHIRAAGPWTTRLREIYSPPTGDTCARYPKLYLDGPFGEGHQEWHKFEVSVLVGAGIGVTPFASILK DLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDSRDLVSVHIYITQLAEKFDLRTTMLYI CERHFQKVLNRSLFTGLRSVTHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINKQD RTHFSHHYENF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_153739
ORF Size	4653 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_153739.1, NP_714961.1
RefSeq Size	5212 bp
RefSeq ORF	4656 bp
Locus ID	266807
UniProt ID	Q8CIY2
MW	177.2 kDa
Gene Summary	human homolog is a NADPH oxidase of the thyroid [RGD, Feb 2006]

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