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Pam (NM_013000) Rat Tagged ORF Clone
CAT＃: RR202803
- TrueORF®
Pam (Myc-DDK-tagged ORF) - Rat peptidylglycine alpha-amidating monooxygenase (Pam), (10 ug)
"NM_013000" in other vectors (3)
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CNY 12,540.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202803 representing NM_013000
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGACGCGCCCGCAGCGGTCTGCTACTGCTGCTGCTGGGGCTGCTCGCCCTGCAGAGCAGCTGCC TGGCCTTCAGAAGCCCACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGATCATTTTCCAATGAATG CCTTGGTACCATTGGACCAGTCACCCCTCTTGATGCATCAGATTTTGCGCTGGATATTCGCATGCCTGGG GTTACACCTAAAGAGTCTGACACATACTTCTGCATGTCCATGCGTCTGCCTGTGGATGAGGAAGCCTTCG TGATTGACTTCAAGCCTCGTGCCAGCATGGATACTGTCCACCATATGCTGCTGTTTGGATGCAATATGCC CTCGTCCACTGGAAGTTACTGGTTTTGTGATGAAGGAACCTGTACAGATAAAGCCAATATTCTATATGCC TGGGCAAGGAATGCTCCCCCCACCCGGCTCCCGAAAGGTGTTGGATTCAGAGTTGGAGGAGAAACTGGAA GCAAATACTTCGTCCTTCAAGTTCACTATGGCGATATCAGTGCTTTTCGAGATAATCACAAAGACTGCTC TGGCGTGTCCGTACATCTCACACGTGTGCCCCAGCCTTTAATTGCGGGCATGTACCTTATGATGTCTGTT GACACTGTCATACCACCAGGAGAGAAAGTAGTGAATGCTGACATTTCGTGCCAATACAAAATGTATCCAA TGCATGTGTTTGCCTACAGAGTCCACACTCACCATTTAGGTAAGGTGGTGAGCGGATACAGAGTAAGAAA CGGACAGTGGACACTGATTGGACGCCAGAACCCCCAGCTGCCACAGGCTTTCTACCCTGTGGAACACCCC GTTGATGTTACTTTTGGTGATATACTGGCAGCCAGATGTGTGTTCACTGGTGAAGGGAGGACAGAGGCCA CCCATATCGGCGGCACTTCTAGTGACGAAATGTGTAACCTGTACATCATGTATTACATGGAAGCCAAATA TGCACTTTCCTTCATGACCTGTACAAAGAACGTGGCTCCAGATATGTTCAGAACTATCCCAGCAGAGGCC AATATCCCAATTCCTGTCAAACCGGACATGGTTATGATGCACGGGCATCACAAAGAAGCAGAAAACAAAG AAAAGAGTGCTTTAATGCAGCAGCCAAAACAGGGAGAGGAAGAAGTATTAGAGCAGGGTGATTTCTATTC ACTGCTTTCCAAGCTGCTAGGAGAAAGGGAAGATGTTCATGTGCACAAGTATAATCCTACAGAAAAGACA GAATCTGGGTCAGACCTGGTAGCTGAGATTGCAAACGTGGTCCAGAAAAAGGACCTTGGTCGGTCTGACG CCAGAGAAGGTGCAGAGCATGAGGAATGGGGTAATGCTATCCTAGTCAGAGACAGGATCCACAGATTCCA CCAGCTAGAGTCAACTCTGAGGCCAGCTGAGAGCAGAGCTTTCTCGTTCCAGCAGCCTGGCGAAGGCCCT TGGGAACCAGAACCCTCAGGAGATTTCCATGTGGAAGAAGAACTGGACTGGCCTGGAGTGTACTTGTTAC CAGGCCAGGTTTCTGGGGTGGCCCTGGATTCTAAGAATAACCTAGTGATTTTCCACAGAGGTGACCATGT TTGGGATGGAAACTCTTTTGACAGCAAGTTTGTTTACCAGCAAAGAGGTCTTGGGCCAATTGAAGAAGAC ACCATCCTGGTCATTGACCCAAATAATGCTGAAATCCTCCAGTCCAGTGGCAAGAACCTGTTTTATTTAC CACACGGCTTGAGCATAGATACAGATGGAAATTATTGGGTCACAGATGTGGCTCTCCACCAGGTGTTCAA ATTGGACCCGCATAGCAAAGAAGGCCCTCTCTTAATTCTGGGAAGGAGCATGCAACCTGGGAGTGACCAA AATCATTTCTGCCAGCCCACCGATGTGGCTGTGGAGCCCAGTACTGGAGCTGTCTTCGTGTCAGACGGTT ACTGTAACAGTCGGATTGTGCAGTTTTCACCAAGCGGAAAGTTCGTCACCCAGTGGGGAGAAGAGTCCTC TGGAAGCAGTCCTAGGCCAGGCCAGTTCAGTGTTCCTCACAGTTTGGCCCTTGTGCCTCATTTGGACCAG TTGTGTGTGGCAGACAGGGAAAATGGCCGAATCCAATGCTTCAAAACTGACACCAAAGAATTTGTGAGAG AGATTAAGCACGCATCATTTGGAAGGAATGTCTTTGCCATTTCATATATACCAGGTTTCCTCTTTGCCGT AAACGGGAAGCCTTACTTTGGAGACCAAGAGCCCGTGCAAGGATTTGTGATGAACTTTTCCAGTGGGGAA ATTATAGACGTCTTCAAGCCAGTACGCAAGCACTTCGACATGCCTCATGATATTGTGGCTTCTGAAGATG GGACTGTGTACATTGGAGACGCACACACAAACACCGTGTGGAAGTTCACCCTGACTGAAAAAATGGAGCA TCGGTCAGTTAAAAAGGCTGGCATTGAAGTCCAGGAAATCAAAGAAGCCGAGGCAGTTGTTGAACCCAAA GTGGAGAACAAACCCACCTCCTCAGAATTGCAGAAGATGCAAGAGAAACAGAAACTGAGCACAGAGCCCG GCTCGGGAGTGTCCGTGGTTCTCATTACAACCCTTCTGGTTATTCCTGTGCTGGTCCTGCTGGCCATTGT CATGTTTATTCGGTGGAAAAAATCAAGGGCCTTTGGAGATCATGACCGCAAGCTCGAGTCAAGTTCTGGA AGAGTCCTGGGAAGATTCCGAGGAAAGGGAAGCGGCGGCTTAAATCTGGGAAATTTCTTTGCAAGTCGAA AAGGCTACAGCAGAAAAGGGTTTGACCGAGTGAGCACAGAGGGGAGTGACCAAGAGAAAGATGAGGACGA CGGAACTGAGTCTGAAGAGGAGTACTCGGCCCCGCTGCCCAAGCCTGCACCTTCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202803 representing NM_013000
Red=Cloning site Green=Tags(s) MAGRARSGLLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFSNECLGTIGPVTPLDASDFALDIRMPG VTPKESDTYFCMSMRLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYA WARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSVHLTRVPQPLIAGMYLMMSV DTVIPPGEKVVNADISCQYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQNPQLPQAFYPVEHP VDVTFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKYALSFMTCTKNVAPDMFRTIPAEA NIPIPVKPDMVMMHGHHKEAENKEKSALMQQPKQGEEEVLEQGDFYSLLSKLLGEREDVHVHKYNPTEKT ESGSDLVAEIANVVQKKDLGRSDAREGAEHEEWGNAILVRDRIHRFHQLESTLRPAESRAFSFQQPGEGP WEPEPSGDFHVEEELDWPGVYLLPGQVSGVALDSKNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEED TILVIDPNNAEILQSSGKNLFYLPHGLSIDTDGNYWVTDVALHQVFKLDPHSKEGPLLILGRSMQPGSDQ NHFCQPTDVAVEPSTGAVFVSDGYCNSRIVQFSPSGKFVTQWGEESSGSSPRPGQFSVPHSLALVPHLDQ LCVADRENGRIQCFKTDTKEFVREIKHASFGRNVFAISYIPGFLFAVNGKPYFGDQEPVQGFVMNFSSGE IIDVFKPVRKHFDMPHDIVASEDGTVYIGDAHTNTVWKFTLTEKMEHRSVKKAGIEVQEIKEAEAVVEPK VENKPTSSELQKMQEKQKLSTEPGSGVSVVLITTLLVIPVLVLLAIVMFIRWKKSRAFGDHDRKLESSSG RVLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRVSTEGSDQEKDEDDGTESEEEYSAPLPKPAPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013000 |
| ORF Size | 2928 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013000.2, NP_037132.2 |
| RefSeq Size | 3770 bp |
| RefSeq ORF | 2931 bp |
| Locus ID | 25508 |
| UniProt ID | P14925 |
| MW | 108.7 kDa |
| Gene Summary | catalyzes conversion of glycine-extended peptides into biologically active alpha-amidated peptides [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202803 | Pam (untagged ORF) - Rat peptidylglycine alpha-amidating monooxygenase (Pam), (10 ug) |
CNY 10,728.00 |
|
| RR202803L3 | Lenti ORF clone of Pam (Myc-DDK-tagged ORF) - Rat peptidylglycine alpha-amidating monooxygenase (Pam), (10 ug) |
CNY 14,250.00 |
|
| RR202803L4 | Lenti ORF clone of Pam (mGFP-tagged ORF) - Rat peptidylglycine alpha-amidating monooxygenase (Pam), (10 ug) |
CNY 15,680.00 |
