Eci2 (NM_001006966) Rat Tagged ORF Clone

CAT#: RR202644

  • TrueORF®

Eci2 (Myc-DDK-tagged ORF) - Rat peroxisomal D3,D2-enoyl-CoA isomerase (Peci), (10 ug)



  "NM_001006966" in other vectors (3)

Need custom modification / cloning service?
Get a free quote

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Peci
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR202644 representing NM_001006966
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGTGACCTGGAGTCGGGCGCGGTGCTGGTGTCCGAGTCTCCTGCAGGTCCTCAGATTACCAG
TGACTAAACTGCACCTAGGCAGACCAGCCATGAGAGCCACTCAGCAGGACTTCGAAAATGCAATGAACCA
GGTGAAACTCTTGAAGAAGGACCCAGGAAATGAAGTGAAGCTGAGACTGTATGCGCTGTATAAGCAGGCC
ACAGAAGGACCCTGCACTATGCCTAAACCAGGTGTGTTTGACTTTGTCAATAAAGCCAAGTGGGATGCAT
GGAATGCTCTGGGCAGCCTACCAAAGGAAACTGCCAGGCAGAACTATGTGGACCTCGTATCCAGTTTGAG
TTCCTCATCTGAAGCCTCGAGCCAGGGAAAGGGTGGAGCTGATGGGAAAGCCCAGGAGTCCAAGGGCATC
CTGGTAACGTCTGAAGGTGGCATCACAAAGATCACGTTCAATCGGCCCTCCAAAAAGAACGCCATAACCT
TCCAGATGTATCAGGATATTATACTCGCGCTTAAGAATGCCAGCACGGATGACACAGTCATCACCGTTTT
CACAGGAGCTGGTGACTACTACAGCAGTGGGAATGACCTGACTAACTTCACTAGCGCCTCTGGGGGAATG
GAGGAGGCGGCTAACAAGGGTGCTATAGTACTGAGGGAATTTGTAAACACTTTTATAGACTTTCCCAAGC
CTCTGGTTGCAGTAGTCAATGGCCCAGCTGTGGGAATCTCTGTCACCCTTCTGGGACTATTTGATGCCGT
CTATGCATCGGACAGGGCAACATTTCATACTCCATTCAGCCACCTCGGCCAGAGCCCGGAAGCCTGCTCC
TCTTACACGTTTCCGAAGATGATGGGCTCAGCAAAGGCAGCTGAGATGCTTCTCTTTGGGAAGAAGCTCA
CAGCAAGAGAGGCTTGGGCTCAAGGCCTTGTCACTGAAGTTTTCCCTGAAAGCACTTTTGAGACAGAAGT
CTGGACCAGGCTGAAAACATATGCGAAGCTCCCGCCAAACTCCATGAGAATTTCCAAAGAGTTAATCAGG
AAGAATGAGAAAGAAAAGCTCCATGCAGTTAACGAGGAGGAGTGCACTACTTTACGAGCAAGGTGGCTGT
CAGAGGAGTGCATCAACGCAATCATGAGCTTTGTTACCAGAAAGCCAAAGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR202644 representing NM_001006966
Red=Cloning site Green=Tags(s)

MAAVTWSRARCWCPSLLQVLRLPVTKLHLGRPAMRATQQDFENAMNQVKLLKKDPGNEVKLRLYALYKQA
TEGPCTMPKPGVFDFVNKAKWDAWNALGSLPKETARQNYVDLVSSLSSSSEASSQGKGGADGKAQESKGI
LVTSEGGITKITFNRPSKKNAITFQMYQDIILALKNASTDDTVITVFTGAGDYYSSGNDLTNFTSASGGM
EEAANKGAIVLREFVNTFIDFPKPLVAVVNGPAVGISVTLLGLFDAVYASDRATFHTPFSHLGQSPEACS
SYTFPKMMGSAKAAEMLLFGKKLTAREAWAQGLVTEVFPESTFETEVWTRLKTYAKLPPNSMRISKELIR
KNEKEKLHAVNEEECTTLRARWLSEECINAIMSFVTRKPKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001006966
ORF Size 1173 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001006966.1, NP_001006967.1
RefSeq Size 1610 bp
RefSeq ORF 1176 bp
Locus ID 291075
UniProt ID Q5XIC0
MW 43 kDa
Gene Summary Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...