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Myl2 (NM_001035252) Rat Tagged ORF Clone
CAT＃: RR202291
- TrueORF®
Myl2 (Myc-DDK-tagged ORF) - Rat myosin, light polypeptide 2, regulatory, cardiac, slow (Myl2), (10 ug)
"NM_001035252" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mlc-2; MLC-2s/v; Mlc2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202291 representing NM_001035252
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCAAAGAAAGCCAAGAAGAGGTTAGAGGGCGGAAGCTCCAACGTGTTCTCCATGTTTGAGCAGA CCCAGATCCAGGAGTTCAAGGAGGCCTTCACAATCATGGACCAGAACAGAGACGGCTTCATTGACAAGAA TGACCTAAGGGACACGTTTGCTGCCCTCGGACGAGTGAACGTGAAAAACGAAGAGATCGATGAGATGATC AAAGAGGCTCCAGGTCCAATTAACTTCACTGTGTTCCTCACCATGTTTGGGGAGAAACTTAAAGGAGCTG ACCCGGAGGAGACCATTCTCAACGCCTTCAAGGTGTTTGACCCCGAAGGCAAAGGGTCGCTGAAGGCCGA CTATGTCCGGGAGATGCTGACCACGCAAGCAGAGAGGTTCTCCAAAGAAGAGATCGACCAGATGTTCGCA GCTTTTCCCCCTGACGTCACCGGCAACCTTGATTATAAGAATTTGGTTCACATCATCACCCACGGAGAAG AGAAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202291 representing NM_001035252
Red=Cloning site Green=Tags(s) MSPKKAKKRLEGGSSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMI KEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGSLKADYVREMLTTQAERFSKEEIDQMFA AFPPDVTGNLDYKNLVHIITHGEEKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001035252 |
| ORF Size | 498 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001035252.2, NP_001030329.2 |
| RefSeq Size | 677 bp |
| RefSeq ORF | 501 bp |
| Locus ID | 363925 |
| UniProt ID | P08733 |
| MW | 18.9 kDa |
| Gene Summary | Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (PubMed:15331360). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202291 | Myl2 (untagged ORF) - Rat myosin, light polypeptide 2, regulatory, cardiac, slow (Myl2), (10 ug) |
CNY 3,990.00 |
|
| RR202291L3 | Lenti ORF clone of Myl2 (Myc-DDK-tagged ORF) - Rat myosin, light polypeptide 2, regulatory, cardiac, slow (Myl2), (10 ug) |
CNY 6,080.00 |
|
| RR202291L4 | Lenti ORF clone of Myl2 (mGFP-tagged ORF) - Rat myosin, light polypeptide 2, regulatory, cardiac, slow (Myl2), (10 ug) |
CNY 6,650.00 |
