Ptpro (NM_017336) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GLEPP1; RPTP-BK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202260 representing NM_017336 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCACCTGCCTAGGGAAAGGCGCGGGGGCCGCCGCCTGCTACCTCTGCTCGGGCTCTTTGTGCTGC TCAAGATTGTTTCAACGTTCCACGTGACTGTCCGAGACGACAACAACATCGTTGTGTCTTTAGAAGCTTC TGATGTAGTCAGCCCAACCTCCGTGTATGTTGTGAGGGTAGCTGGCGAATCCAAAAACTATTTCTTCGAA TTTGAGGAATCCAATGGCACACTGCCTCCTCCTGTGGTCTTTAAGGCCACGTATCATGGTCTTTATTACA TCATCAGTCTGGTGGTAGTCAGTGGGAATGTGGTCACCAAACCATCCCGATCAATCACCGTGCTGACAAA ACCCTTGCCCGTAACCAGTGTGTCTATATATGACTATAAGCCTTCTCCTGAGACAGGAGTACTGTTTGAA ATTCACTATCCGGAAAAATACAACGTGTTCAGCAGAGTGAACATCAGCTACTGGGAGGGGAAGGATTTCA GGACGATGCTGTACAAAGACTTCCTGAAGGGGAAAACCGTGTTTAATCACTGGCTCCCGGGGATCTGCTA CAGTAACATCACTTTCCAGCTGGTTTCTGAGGCGACCTTTAACAAAAGCACCCTGGTGGAGTGCAGTGGT GTGAGTCACGAACCCAAACAACACAGAACAGCGCCGTATCCCCCTCGAAACATCTCTGTTCGCTTTGTCA ACTTGAACAAGAACAACTGGGAAGAACCAAGCGGGAGCTTCCCCGAGGACTCGTTCATCAAACCGCAAGA CGCGATAGGAAGAGACAGACCCTTCCATTTCCCCGAGGAAACCCCTGAGACGCCCGCCAGCAATGCGTCT TCCGGCTGGCCTGACCCGAACGGCACTGAGTATGAGAGCACATCTCAGCCCTACTGGTGGGACAGTGCAT CCGCGGCCCCTGAAAACGAGGAGGACTTCGTCAGCGTGCTGCCAGCGGACTATGACACTGAGGCCACGCT CGGCAGGACAGAGAAACCCACAGCTGACCCTTTCTCTGCCTTCCCCGTGCAGATGACTCTGAGCTGGTTA CCACCAAAACCGCCTACAGCCTTCGATGGCTTCAATATCCTCATAGAGAGGGAAGGGAACTTTACCGACT ATTTGACAGTGGATGAAGAAGCCCACGAGTTTGTTGCCGAACTGAAGGAGCCTGGAAAGTACAGACTCTC AGTGACAACCTTCAGCTCCTCGGGAGCCTGTGAGACTCGGAAAAGCCAGTCAGCAAAATCGCTCAGCTTC TACATCAGCCCCACAGGAGAGTGGATTGAAGAACTGACTGAGAAACCGCAGCACGTGAGTGTCCACGTCT TAAGCTCAACCACTGCCTTGTTGTCGTGGACATCTTCCCAGGAGAACTACAACAGCACCATTGTGTCTGT GGTGTCCCTGACATGCCAGAAACAGAAAGAGAGCCAGCGGCTGGAGAAGCAGTATTGTACCCAGGTGAAC TCAAGCAAACCTGTAATTGAGAACCTGGTTCCCGGTGCCCAGTACCAGGTTGTGATGTACCTAAGAAAAG GCCCTTTGATTGGGCCACCTTCTGATCCTGTGACATTTGCCATTGTTCCCACGGGGATAAAGGATTTAAT GCTCTATCCATTGGGTCCCACGGCAGTAGTGCTGAGCTGGACCCGACCTTACTTGGGAGTGTTCAGAAAA TACGTGGTTGAAATGTTTTATTTCAACCCCACCACCATGACCTCCGAGTGGACGACCTACTACGAAATAG CAGCGACCGTCTCCTTAACATCGTCCGTGAGAATAGCAAATCTATTGCCGGCGTGGTACTACAACTTTCG CGTAACCATGGTGACGTGGGGAGATCCAGAACTGAGCTGCTGTGACAGTTCCACCATCAGCTTCATAACG GCCCCCGTCGCTCCAGAAATCACGTCTGTGGAGTACTTCAACAGCCTGCTGTACATCAGCTGGACCTACG GGGACGCCACCACTGACCTGTCCCATTCGAGAATGCTACACTGGATGGTCGTGGCAGAAGGGAGGAGGAA AATTAAAAAGAGTGTGACACGCAATGTCATGACGGCCATCCTTAGCCTGCCTCCAGGAGATATCTACAAC CTGTCTGTCACAGCCTGCACTGAGAGGGGGAGTAACACCTCCATGCTCCGCCTTGTCAAGCTAGAACCAG CTCCTCCAAAATCACTCTTCGCAGTGAACAAAACGCAGACATCGGTCACCCTGCTTTGGGTTGAGGAGGG CGTAGCTGATTTCTTTGAAGTCCTCTGTCAGAAGCTCGGGTCTGGCCACGATGGCAAACTCCAGGAACCG GTAGCTGTTTCCTCCCACGTGGTGACCATCTCCAGCCTCCTCCCAGCCACTGCCTACAACTGCAGTGTCA CCAGCTTCAGCCACGACAGTCCCAGTGTCCCTACGTTCATAGCTGTCTCCACAATGGTTACAGAGGTGAA CCCTAATGTGGTGGTGATCTCGGTGCTGGCCATCCTCAGCATACTTTTAATTGGACTGCTGTTAGTGACC CTTGTCATTCTGAGAAAGAAGCACCTGCAGATGGCCAGGGAGTGTGGGGCTGGCACGTTTGTCAATTTTG CATCCTTGGAGAGGGAAGGGAAACTCCCCTACAGTTGGCGTAGGAGTGTCTTTACTTTCTTAACCCTGCT GCCCTCATGTCTGTGGACTGACTATCTGTTGGCATTTTATATTAACCCTTGGAGTAAAAATGGCTTAAAG AAGAGGAAACTGACGAACCCTGTTCAGCTGGACGATTTTGATTCTTACATCAAGGACATGGCCAAGGACT CTGACTATAAATTCTCTCTTCAGTTTGAGGAGTTGAAGTTGATTGGACTGGATATTCCACACTTTGCTGC AGATCTTCCGCTGAACCGATGTAAAAACCGCTATACAAACATCCTGCCGTACGACTTCAGCAGAGTGAGG CTGGTCTCCATGAATGAAGAAGAAGGAGCAGACTACATTAATGCCAATTATATTCCTGGCTACAACTCAC CTCAGGAGTACATTGCCACCCAGGGACCACTGCCGGAAACCAGAAATGATTTCTGGAAGATGGTCCTGCA GCAGAAGTCTCGCATCATTGTCATGCTCACTCAGTGCAATGAGAAAAGAAGGGTAAAATGCGACCACTAC TGGCCATTCACGGAAGAACCCATTGCTTATGGAGACATCACCGTGGAGATGGTCTCTGAGGAAGAACAGG AGGACTGGGCCAGCAGACACTTCCGGATCAATTATGCTGACGAGGCCCAGGATGTGATGCACTTTAACTA CACGGCCTGGCCAGACCATGGCGTGCCTCCAGCAAATGCTGCCGAGAGCATCCTGCAGTTCGTGTACACT GTCCGGCAGCAAGCCACCAAGAGCAAAGGACCCATGATCATCCACTGCAGCGCGGGCGTGGGACGGACAG GCACCTTCATAGCCCTTGACAGGCTCTTGCAACACATCCGAGATCACGAATTTGTGGACATCTTGGGGCT GGTGTCAGAAATGCGCTCATACCGAATGTCGATGGTACAGACAGAGGAGCAGTACATCTTCATCCATCAG TGCGTGCAGCTGATGTGGCTGAAGAAGAAGCAACAGTTCTGCATCAGTGACGTCATCTACGAGAACGTCA GCAAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202260 representing NM_017336 Red=Cloning site Green=Tags(s) MGHLPRERRGGRRLLPLLGLFVLLKIVSTFHVTVRDDNNIVVSLEASDVVSPTSVYVVRVAGESKNYFFE FEESNGTLPPPVVFKATYHGLYYIISLVVVSGNVVTKPSRSITVLTKPLPVTSVSIYDYKPSPETGVLFE IHYPEKYNVFSRVNISYWEGKDFRTMLYKDFLKGKTVFNHWLPGICYSNITFQLVSEATFNKSTLVECSG VSHEPKQHRTAPYPPRNISVRFVNLNKNNWEEPSGSFPEDSFIKPQDAIGRDRPFHFPEETPETPASNAS SGWPDPNGTEYESTSQPYWWDSASAAPENEEDFVSVLPADYDTEATLGRTEKPTADPFSAFPVQMTLSWL PPKPPTAFDGFNILIEREGNFTDYLTVDEEAHEFVAELKEPGKYRLSVTTFSSSGACETRKSQSAKSLSF YISPTGEWIEELTEKPQHVSVHVLSSTTALLSWTSSQENYNSTIVSVVSLTCQKQKESQRLEKQYCTQVN SSKPVIENLVPGAQYQVVMYLRKGPLIGPPSDPVTFAIVPTGIKDLMLYPLGPTAVVLSWTRPYLGVFRK YVVEMFYFNPTTMTSEWTTYYEIAATVSLTSSVRIANLLPAWYYNFRVTMVTWGDPELSCCDSSTISFIT APVAPEITSVEYFNSLLYISWTYGDATTDLSHSRMLHWMVVAEGRRKIKKSVTRNVMTAILSLPPGDIYN LSVTACTERGSNTSMLRLVKLEPAPPKSLFAVNKTQTSVTLLWVEEGVADFFEVLCQKLGSGHDGKLQEP VAVSSHVVTISSLLPATAYNCSVTSFSHDSPSVPTFIAVSTMVTEVNPNVVVISVLAILSILLIGLLLVT LVILRKKHLQMARECGAGTFVNFASLEREGKLPYSWRRSVFTFLTLLPSCLWTDYLLAFYINPWSKNGLK KRKLTNPVQLDDFDSYIKDMAKDSDYKFSLQFEELKLIGLDIPHFAADLPLNRCKNRYTNILPYDFSRVR LVSMNEEEGADYINANYIPGYNSPQEYIATQGPLPETRNDFWKMVLQQKSRIIVMLTQCNEKRRVKCDHY WPFTEEPIAYGDITVEMVSEEEQEDWASRHFRINYADEAQDVMHFNYTAWPDHGVPPANAAESILQFVYT VRQQATKSKGPMIIHCSAGVGRTGTFIALDRLLQHIRDHEFVDILGLVSEMRSYRMSMVQTEEQYIFIHQ CVQLMWLKKKQQFCISDVIYENVSKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017336
ORF Size	3648 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017336.1, NP_059032.1
RefSeq Size	4871 bp
RefSeq ORF	3651 bp
Locus ID	50677
MW	137.6 kDa
Gene Summary	may regulate glomerular epithelial cell function; may play a role in neural cell development [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.