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Csk (NM_001030039) Rat Tagged ORF Clone
CAT＃: RR202254
- TrueORF®
Csk (Myc-DDK-tagged ORF) - Rat c-src tyrosine kinase (Csk), (10 ug)
"NM_001030039" in other vectors (3)
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CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC112926 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202254 representing NM_001030039
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCTATACAGGCCTCCTGGCCATCCGGTACAGAATGTATTGCCAAGTACAACTTCCATGGCACTG CCGAGCAAGACCTTCCCTTCTGCAAAGGAGATGTGCTCACCATTGTGGCTGTCACCAAGGACCCCAACTG GTACAAAGCCAAAAACAAAGTGGGCCGTGAGGGCATCATCCCAGCCAACTATGTCCAGAAGCGTGAGGGT GTGAAGGCAGGCACCAAGCTCAGCCTTATGCCCTGGTTCCACGGCAAGATCACACGGGAGCAGGCGGAGC GGCTTCTCTACCCACCAGAGACAGGCCTGTTCCTGGTGCGGGAAAGCACCAACTACCCTGGGGACTACAC ACTGTGTGTGAGCTGTGAAGGCAAGGTGGAGCACTACCGCATCATGTATCACGCGAGCAAGCTGAGCATT GATGAGGAGGTGTACTTCGAGAACCTCATGCAGCTGGTGGAGCACTACACCACAGATGCCGACGGACTCT GCACTCGCCTCATCAAACCAAAGGTCATGGAGGGCACAGTGGCGGCCCAAGATGAATTCTACCGCAGTGG CTGGGCCCTGAACATGAAGGAACTGAAGCTGCTACAGACGATAGGAAAGGGGGAGTTTGGAGATGTGATG CTGGGGGATTACCGAGGCAACAAAGTTGCAGTCAAGTGCATTAAGAATGATGCTACAGCCCAGGCCTTCC TGGCTGAAGCCTCTGTCATGACGCAGCTTCGGCACAGCAACCTAGTCCAGCTACTGGGTGTGATTGTGGA GGAGAAGGGTGGGCTCTACATCGTCACAGAGTACATGGCCAAGGGGAGTTTGGTGGACTATCTTCGATCA CGTGGTCGTTCGGTGCTAGGCGGAGACTGTCTCCTCAAATTCTCACTAGACGTCTGTGAAGCCATGGAGT ACCTGGAGGGTAACAATTTTGTGCACCGGGACTTGGCTGCCCGGAATGTGCTGGTGTCTGAGGACAACGT GGCCAAAGTCAGTGACTTTGGCCTCACTAAGGAAGCTTCCAGCACTCAGGACACAGGCAAACTGCCAGTC AAGTGGACAGCTCCTGAAGCCTTGAGAGAGAAGAAATTTTCCACCAAGTCTGATGTGTGGAGTTTCGGAA TCCTTCTCTGGGAAATCTATTCCTTCGGGCGAGTGCCTTACCCAAGAATTCCCCTGAAGGACGTCGTCCC TCGGGTGGAAAAGGGCTATAAGATGGACGCTCCGGATGGCTGCCCACCCGCAGTCTATGATGTTATGAAG AACTGCTGGCACCTGGATGCTGCCACGCGGCCCACCTTTCTGCAGCTTCGAGAGCAGCTCGAGCACATCA GAACCCATGAGCTGCACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202254 representing NM_001030039
Red=Cloning site Green=Tags(s) MSAIQASWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREG VKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCEGKVEHYRIMYHASKLSI DEEVYFENLMQLVEHYTTDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVM LGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRS RGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPV KWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYDVMK NCWHLDAATRPTFLQLREQLEHIRTHELHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001030039 |
| ORF Size | 1350 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001030039.1, NP_001025210.1 |
| RefSeq Size | 2108 bp |
| RefSeq ORF | 1353 bp |
| Locus ID | 315707 |
| UniProt ID | P32577 |
| MW | 50.7 kDa |
| Gene Summary | Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202254 | Csk (untagged ORF) - Rat c-src tyrosine kinase (Csk), (10 ug) |
CNY 5,800.00 |
|
| RR202254L3 | Lenti ORF clone of Csk (Myc-DDK-tagged ORF) - Rat c-src tyrosine kinase (Csk), (10 ug) |
CNY 7,700.00 |
|
| RR202254L4 | Lenti ORF clone of Csk (mGFP-tagged ORF) - Rat c-src tyrosine kinase (Csk), (10 ug) |
CNY 8,460.00 |
