Kdm6b (NM_001108829) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Jmjd3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202151 representing NM_001108829 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCTGCCGCACTCTCAGGCCCCTCAGGAGAGGAAGGCCTCAGCCCTGGAGGCAAGCGCAGGAGAG GCTGCAGCTCTGAACAGGAGCAGCGGCACTCGCTGCCTCACCCTCATCCATACCCAGCTCCTGCCTACAC TGCTCATCCGCCCAGCCACCGGCTGGGCCCGGCCACACCCCTTGGTCCAGGTCCCCGACCCCCAGGAGCA GAGAGCCATGGCTGCCTGCCTGCCACCCGTCCCCCCGGAAGTGACCTTAGAGAGAGCAGAGTTCAGAGGT CGCGGATGGACTCCAGCGTTTCACCAGCAGCATCTACCGCCTGCGTGCCTTACGCCCCTTCCCGGCCCCC TGGCCTCCCCGGCACCAGCAGCAGCAGCAGTAGCAGTAACAACACTGGTCTTCGGGGTGTGGAGCCGAGC CCAGGCATTCCTGGCGCTGACCATTACCAAAACCCTGCGTTGGAGATAGCCCCTCACCAGGCCCGCCTGG GTCCCTCCGCACACAGCAGTCGGAAACCATTTTTGACAGCCCCTGCTGCCGCTCCCCACTTATCCCTACC ATCCGGGACCCCTTCATCCCCTCCACCCCCATGTCCTCGCCTCTTGCGCCCTCCACCACCCCCTGCTTGG ATGAAGGGCTCAGCCTGCCGCTCAGCTCGAGAGGATGGAGAGATCTTAGGGGAGCTCTTCTTTGGTGCTG AGGGACCTCCCCGTCCTCCTCCCCCACCCCTTCCCCACCGTGATGGCTTCTTGGGGCCTCCAAACCCCCG CTTTTCTGTGGGCACTCAGGATTCGCATACCCCTCCCACTCCCCCAACCACCACCAGCAGCAGCAGCAGC AGCAGCAGCAGCAACAGCCACAGCAGTAGTCCTACTGGGCCTGTGCCCTTCCCACCACCTCCCTATCTGG CCAGAAGTATAGACCCCCTTCCCAGGCCATCTAGCCCAACACTGAGCTCCCAGGACCCACCTCTTCCACC ACTGACTCTTGCCCTGCCTCCAGCCCCTCCCTCCTCCTGCCACCAAAATACCTCAGGAAGCTTCAGGCGC TCGGAGAGCCCCCGGCCCAGGGTCTCCTTCCCAAAGACCCCCGAGGTGGGGCAGGGGCCACCTCCAGGCT CTCTGAGTAAAGCCCCCCAGCCTGTGCCACCTGGGGTTGGAGAGCTGCCTGCCCGAGGCCCGAGGCTCTT TGATTTTCCACCAACTCCACTGGAGGACCAGTTTGAAGAGCCAGCTGAATTCAAGATCCTCCCTGATGGG CTAGCTAACATCATGAAGATGCTGGATGAATCCATTAGAAAGGAGGAAGAGCAGCAGCAGCAAGAGGCAG GCGCGGTTCCCCCACCCCCACTCAAAGAGCCCTTTGCATCTCTACGGTCTTCACTTCCCAGTGACACAGC CCCAGCTGCCACCACTGCTGCTGCAACTCCCACCACCACCACCACCACCACCACCCAAGAAGAGGAGAAG AAGCCACCACCAGCCCTGCCACCACCACCGCCTCTAGCCAAGTTTCCTCCACCACCCCAGCCCCCACCAC CTCCACCAGCCAGCCCAGCCAGCCTGCTCAAATCATTGGCCTCTGTTCTTGAGGGGCAAAAGTACTGTTA TCGGGGGACTGGAGCAGCCGTTTCAACCAGGCCCGGGTCCGTGCCCGCCACTCAGTATTCCCCTAGTCCT GCATCAGGTGCTACCGCCCCACCACCCACTTCAGTGGCCCCTAGCGCCCAGGGCTCCCCCAAGCCCTCTG TTTCCTCGTCATCTCAGTTCTCTACCTCAGGTGGGCCTTGGGTCCGAGAGCGCAGGGCAGGTGAAGAGCC AGCACCAGGCCCCGTGACCCCTGCCCAGTTGCCCCCACCTCTGCCGCTGCCCCCTGCTCGTTCTGAGTCT GAGGTGCTAGAAGAAATCAGTCGGGCTTGTGAGACCCTTGTAGAGCGGGTGGGCCGGAGTGCCATCAACC CAGCAGACCCAGTGGACATAGCAGACCCAGTGGACAGTGGGACTGAGCCACAGCCGCCTCCTGCACAGGC CAAGGAGGAGAGTGGGGGAGTGGCAGTGGCGGCAGCAGGTCCAGGTAGTGGTAAGCGTCGTCAGAAGGAG CATCGGAGGCACAGACGGACCTGTAGGGACAGTGTGGGTCGTAGGCCCCGTGAGGGGAGGGCCAAGGCCA AGGCCAAGGCTCCCAAAGAAAAAAGCCGAAGGGTGCTGGGGAACTTGGACTTGCAGAGTGAAGAGATCCA GGGCCGGGAGAAGGCCCGGCCTGATGTAGGTGGGGCTTCCAAAGTCAAGACGCCCACAACTCCAGCGCCC CCACCTGCTCCTGCCCCCTCTGCTCAGCCAACACCCCCATCAGCTCCTGTCCCTGGGAAGAAGACTCGAG AGGAAGCTCCAGGGCCTCCAGGTGTGAGCCGTGCAGATATGCTGAAGCTTCGGTCGCTTAGTGAGGGGCC ACCTAAGGAGCTGAAGATCAGGCTCATCAAGGTGGAGAGTGGGGACAAGGAGACCTTCATCGCCTCTGAG GTAGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGCGCTGCTGATGTCATGCGTGCCA GCAAGAATGCCAAGGTGAAAGGGAAATTCCGAGAGTCCTACCTTTCCCCTGCCCAGTCTGTGAAACCGAA GATCAACACTGAGGAGAAGCTGCCCCGGGAAAAACTCAACCCTCCTACACCCAGCATTTATTTGGAGAGC AAACGAGACGCCTTCTCGCCGGTCCTGCTGCAGTTCTGTACAGATCCCCGGAACCCCATCACTGTCATCA GGGGCCTGGCAGGTTCACTTCGGCTCAACTTAGGCCTTTTTTCCACCAAGACTCTGGTGGAGGCGAGCGG TGAACACACAGTGGAGGTCCGTACCCAAGTGCAGCAGCCCTCAGACGAGAACTGGGATCTGACAGGTACC AGACAGATCTGGCCCTGTGAGAGCTCCCGTTCACACACCACCATCGCTAAATATGCACAGTACCAGGCTT CGTCCTTCCAGGAGTCACTGCAGGAGGAGAAAGAGAGTGAGGATGAGGAATCCGAGGAACCAGACAGCAC TACAGGAACCTCTCCCAGCAGTGCACCAGACCCCAAGAACCATCACATCATCAAGTTTGGCACAAACATC GACCTGTCTGATGCCAAGAGGTGGAAGCCACAACTACAGGAGCTGCTGAAACTGCCTGCCTTCATGCGAG TAACATCCACAGGCAACATGCTTAGCCATGTGGGACACACCATCCTGGGCATGAACACCGTGCAGCTATA CATGAAGGTTCCTGGCAGTCGAACGCCAGGCCACCAGGAGAATAACAATTTTTGCTCTGTCAATATCAAC ATTGGCCCCGGGGACTGCGAGTGGTTCGCGGTACATGAGCACTATTGGGAGACCATCAGCGCCTTCTGCG ATCGGCATGGTGTGGATTACTTGACTGGTTCCTGGTGGCCAATCTTGGATGACCTCTATGCGTCCAATAT TCCTGTTTACCGCTTCGTGCAGCGCCCTGGAGACCTTGTGTGGATTAATGCAGGGACTGTGCATTGGGTG CAGGCTACTGGCTGGTGCAACAACATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAATACCAGCTGG CCCTGGAGCGATATGAGTGGAACGAGGTGAAGAACGTCAAGTCCATTGTACCCATGATTCATGTGTCCTG GAACGTAGCTCGAACGGTCAAGATCAGTGATCCTGACTTGTTCAAAATGATCAAGTTCTGCCTCCTGCAG TCAATGAAGCACTGCCAGGTGCAGCGGGAGAGCCTGGTGCGGGCAGGGAAGAAAATCGCTTACCAAGGCC GCGTCAAGGACGAGCCTGCCTACTACTGCAACGAATGCGATGTGGAGGTGTTCAACATCCTGTTCGTTAC AAGTGAGAATGGCAGCCGAAACACGTACCTGGTGCACTGCGAGGGCTGTGCGCGCCGTCGCAGTGCGGGC CTACAGGGCGTGGTGGTGCTAGAGCAGTACCGCACAGAGGAGCTGGCACAGGCCTATGATGCCTTCACAC TGGCTCCTGCCAGCACGTCGCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202151 representing NM_001108829 Red=Cloning site Green=Tags(s) MPPAALSGPSGEEGLSPGGKRRRGCSSEQEQRHSLPHPHPYPAPAYTAHPPSHRLGPATPLGPGPRPPGA ESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSNNTGLRGVEPS PGIPGADHYQNPALEIAPHQARLGPSAHSSRKPFLTAPAAAPHLSLPSGTPSSPPPPCPRLLRPPPPPAW MKGSACRSAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHTPPTPPTTTSSSSS SSSSNSHSSSPTGPVPFPPPPYLARSIDPLPRPSSPTLSSQDPPLPPLTLALPPAPPSSCHQNTSGSFRR SESPRPRVSFPKTPEVGQGPPPGSLSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDG LANIMKMLDESIRKEEEQQQQEAGAVPPPPLKEPFASLRSSLPSDTAPAATTAAATPTTTTTTTTQEEEK KPPPALPPPPPLAKFPPPPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSP ASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWVRERRAGEEPAPGPVTPAQLPPPLPLPPARSES EVLEEISRACETLVERVGRSAINPADPVDIADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKE HRRHRRTCRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGASKVKTPTTPAP PPAPAPSAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASE VEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLES KRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGT RQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNI DLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNIN IGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWV QATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQ SMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAG LQGVVVLEQYRTEELAQAYDAFTLAPASTSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108829
ORF Size	4083 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108829.1, NP_001102299.1
RefSeq Size	4679 bp
RefSeq ORF	4086 bp
Locus ID	363630
MW	146.3 kDa

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