Shprh (NM_001107470) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202093 representing NM_001107470 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGCCGAAGGAAACGTGCTCCCCCGATGAAGGTGGATGAAGAAAGGCAGCAGCAGCTTCGCTGGA ACATGCACGAGGACCTGAGGAATGAGCCGCTCAGCTTGACTGAGGAAGAGCGGGCCTGCTGGGATGCAGA CTCCTCCGATTGCATCATCATAGATGATGACCCTCCCGAAGGTGCAGCTCACAGAGATAAGAAGAGGTGT ACAGAGTCTGTGAGCATCTTAAACTCCACTGAAAAAGAGACCCCTCTGTCCGTGGCCTTGAATGTTACCA TTTCTCCCCATCCTTCCGATGATTCTTGGAAAGCGTTTCTGGGAGATTTTGTGCTTCAGCTTCTCCCTGA AGAGAGTTTAGTTGAACATTTTTCTGAAAGGACTTTTACGTTGATGAGTTCAGAGTCAAGCAGTCAGCTC CTGATCTATGTTCATTCAGAATGTAACAATGTGGAGAAAGAAGAAAACGTACTTAAAGGACCAGCAAGTA TCTATGGCAAGGGTATTCGAGTGGAATCCTCCTTCAGTAGTGACATGTTACAGGATTTGGCCTGGCTACA GAAGAGAAGAGGAATAAAACTCTACCAGCGACCAGAAGGAACTCATACGATCAAGGTTAGAATTTACCTC TTGGAAGCTGGCCTGACAAGACTAGACTTCATGAGTGATGCAGGCTCCAGAATGAAAAAGTTCAATCAGC TCCTGAAGAGAGTGATGGGAAAGTTATACAGTCTCAGTATTCCAGATGTGTTGGAAGAAGATGAGGAGGA ATCAGAGAGTGAGCCAGAGGGCCAGGACATTGATGAGCTCTATCAGTTTGTGAAGCGAACACACCAGCAA GAGGCAAAGTCAGTCCAAGTGGATGTCCAGCACCCTGCACTGATTCCGGTGCTGAGGCCGTACCAGAGAG AGGCAGTCAACTGGATGCTACAGCAGGAGCAGTTCAGGAGCACTCCTGCTGATAACTCCCTGCACTTCTT GTGGCGAGAAATTGTTACACCTGATGGCTTGAACCTCTACTATAATCCGTACACAGGCTGCATCATTCGG GACTTCCCACATGCTGGGCCACAGTTGCTCGGTGGAATCTTAGCAGATGAGATGGGCCTCGGGAAGACAG TGGAGGTTCTGGCTCTGATTCTGACTCATACCCGACAAGATGTCAGACAGGATGCCCTCACCCTGCCTGA GGGAAAAGTGGTAAATTATTTCATCCCAACTCATTGTCCTAGAGGAAAAGTGAAAAGTAGAGAAATCCAG AATACAGAGTGTGAACCAAAAGAGAAAGTTCACTGCCCTCCCACACGTGTGATGATTCTGACAGCTGTGA AAGAAATGAATGGGAAAAAAGGCGTTTCTATTCTTTCTATCTATAAGTATGTCAGCTCCACATTCAGATA CGACGTTCAGCGGAACCGGAGTCTTCTGAAGCGGATGTTGAAATGTTTAATCTTTGAAGGTCTTGTGAAA CAGATTAAAGGCCGTGGTTTCTCTGGGACTTTTACACTGGGAAAGAATTACAAAGAAGATGTATTTGATA AAACGAAAAAGCAGGCAGTAGGGAGTCCACAAAAAATTGAGAAAGAGTTAAGGAAATCAGTGAACAAGGA TACAGATTCTGAATACATGCCATCAGACATTTATGATGACGATAATGACAACGACGATGACGACGACGAT GATCCTTATTATTATTACTGCAAGGCAAGAAAAAGCCGTAAGTTGAAGAAGAAGCCTGTGTTACTCACTA AAAAGGAAAAGGGTCAGCCTGTCAATCTGGATTCCCAAGTTGAGGCTCCAGCTGCTGGTGACTGTGCAAG CGCCGATGCTCATGTGTCAGAAAGTACATGTGTCTCTGAAAACAGGCAGATCCAAGACGCAGAGGACTGT GTTGAGTCTCCAAGCCCGGCCGCCATGGAGCTGGCACAGTCTAACACTGCAAGTTTCTGTGAGACCCCTG ACTACCGCTTTGAGTGTATATGTGGTGAATTTGACCAGATCGGCCGCAAGCCAAGAGTTCAGTGTCTGAA GTGTCACCTGTGGCAGCATGCAAAGTGTGTGAATTACGAGGAGAAGAATCTGAAGGTTAAGCCTTTTTAC TGCCCTCATTGCCTGGTTGCAATGGAGCCAGTGTCAACCAGAGCGACTCTCATCATCTCCCCAAGCTCCA TTTGCCACCAGTGGGTAGATGAAATCAACCGGCACGTGAGATCGTCATCCCTTCGAGTTTTGGTATATCA AGGAGTGAAGAAAGATGGGTTCTTACAGCCTCATTTTTTGGCAGAACAAGATATAGTTATCATTACCTAT GATGTTCTCCGTTCAGAATTAAACTATGTCGATATCCCACATAGCAATAGTGAAGATGGGCGTCGCCTGA GGAACCAGAAGCGCTACATGGCCATTCCCAGCCCCCTGGTAGCGGTGGAGTGGTGGCGGATCTGTCTGGA TGAAGCGCAGATGGTCGAGTGTCCTACAGTAAAAGCTGCAGAAATGGCCCAGCGTTTGAGTGGCATTAAT CGGTGGTGTGTCAGTGGCACTCCAGTGCAGAGAGGATTGGAAGATCTTTTTGGGCTGGTGGTCTTTCTTG GGATTGAACCTTATTGTGTCAAACACTGGTGGATTCGACTTCTCTATCATCCATACTGCAAGAAAAATCC TCAACACCTGTACAGTTTTATTGCTAAGATAATGTGGAGGTCCGCAAAGAAAGATGTCATTGACCAAATC CAGATCCCACCTCAGACTGAAGAAATGCACTGGCTCCATTTTTCCCCTGTGGAGAGGCATTTCTACCACC GCCAGCATGAGGTGTGCTGCCAGGATGCTGTGGTAAAGCTCAGGAAGATCTCGGACTGGGCCCTGAAGCT GAGCAGCTTGGACAGAAGGACGGTCTCCTCCATCCTGTACCCCCTGCTGAGACTCAGGCAGGCCTGCTGC CACCCACAGGCTGTGCGTGGGGAGTTCCTGCCACTCCAGAAGAGCACCATGACGATGGAGGAGTTGCTGA CATCTTTGCAGAAGAAGTGTGGAACAGAGTGTGAAGAGGCCCACCGGCAGCTAGTTTGTGCCCTTAACGG CTTAGCAGGCATCCACATCATTAAAGGTAGAAGAGCACAGTACAGAGCAGCCCTTGCTTTGCCTCCCCCA GAATGTGTCATTCTTAGTGAATACGCCTTGGCAGCGGAACTCTACAGAGAAGTTTTAAGGTCGTCTGAGG AGCACAAAGGAAAACTCAAAACTGATTCACTTCAAAGACTTCACGCCACACATAACCTGATGGAGCTCCT GGTAGCCAAACACCCAGGGATACCGCCCACCCTGAGAGATGGCAGACTTGAAGAAGAGGCCAAGCAGCTC CGAGAGCACTACATGAGCAAGTGTAACACAGAGGTGGCCGAAGCCCAGCAAGCTTTGCAGCCTGTGCAGC AGTCCATTCGGGAGCTCCAAAGGAAGGTTCATTCTGGTTCTCCTTGGTGGCTGAATGTAGTTCACAGAGC AATGGAGTATGCTGTTGACGAGGAGCTTGTTCAGCGCGTGCGAAATGAGATAAGCAGTAACTACAAGCAC CAAACGGACAAACTCTCCATGTCAGAGAAGTTCCGTGACTGTAGAGGTCTTCAGTTCTTACTTACAACAC AAATGGAAGAGCTGCACAAGTTCCAGAAGCTAGTGAGGGAGGCTGTGAAGAAGCTGGAGAAACCTCCGTC CCGGGATGTGATTGAGTCTGCAACAGTTTGCCACCTGCGGCCAGCTAGACTCCCTCTCAACTGCTGTGTC TTTTGTAAAGCTGATGAATTGTTCACAGAGTATGAGTCAAAGCTATTTTCTAACACTGTCAAAGGCCAGA CCGCCATCTTTGAAGAAATGATAGAAGATGAAGAAGGACTCGTGGATGATAGAGTCCCTACCACTACCAG AGGCCTGTGGGCCGTGAGCGAGACGGAGCGATCTATGAAAGCAATACTATCATTTGCAAGATCACATAGG TTTGACGTTGAATATATAGATGAAGGAAGTGTTTCAATGGATCTCTTCGAGGCATGGAAGAAGGAGTATA AGTTACTTCATGAATACTGGATGACTCTGAGAAACCGCGTATCCGCTGTTGATGAACTTGCAATGGCTAC AGAGCGACTAAGAGTGCGCCATCCCAAGGAGCCAAAGCCAAACCCACCCGTCCACCACATCATTGAACCA CACGAGGTTGAACAGAATCGTATAAAACTAGTAAATGATAAAGCTGTTGCTACTTCCCAGCTTCAGAAGA AACTTGGACAGCTTCTTTACCTGACAAATTTGGAGAAGTCTCAGGACAAAACATCAGGAGGTATTAATCC AGAGCCTTGTCCGATCTGTGCTCGGCAGTTGGGAAAACAGTGGGCAGTGCTGACCTGCGGACACTGCTTC TGTAATGAATGCACTTCAATTATAATTGAACAATACAGTGTGGGATCCCATCGAAGCTCCATTAAGTGTG CTATCTGCCGCCAGACCACATCACACAAAGAAGTCTCCTATGTCTTCACTTCAGAGAAGGCAAACCAAGA GGATGATATCCCTGTGAAGGGTAGCCATTCTACAAAAGTGGAAGCTGTGGTCAGAACTCTGATGAAAATA CAACTTAAAGATCCGGGGGCCAAAGCGCTCGTTTTCTCAACGTGGCAAGATGTGTTAGATATTATTTCAA AAGCTCTCACGGACAACAACATGGGATTCACACAAATCAGTCGCATTAAGACATTCCAGGAGAACCTTTC AGCATTTAAATATGATCCCCAGATCAATATTTTGCTGCTGCCCCTGCATACAGGCTCTAATGGATTAACT ATCATTGAAGCCACTCATGTCCTCCTGGTGGAACCGATACTGAATCCTGCCCATGAGCTTCAGGCCATTG GGAGAGTGCACCGAATTGGACAGACAAAACCTACTATTGTACATAGATTCTTAATTAAAGCAACAATAGA AGAGCGGATGCAGGCAATGCTAAAGACTGCTGAGAGGAGCCACACCAGCTCGTCTGGGAAGCATTCTGAA GCCTCTGCACTGACCGTGGCTGACCTGGCAGACCTGTTCACCAAAGAGAGTGAGGAGCTTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202093 representing NM_001107470 Red=Cloning site Green=Tags(s) MSSRRKRAPPMKVDEERQQQLRWNMHEDLRNEPLSLTEEERACWDADSSDCIIIDDDPPEGAAHRDKKRC TESVSILNSTEKETPLSVALNVTISPHPSDDSWKAFLGDFVLQLLPEESLVEHFSERTFTLMSSESSSQL LIYVHSECNNVEKEENVLKGPASIYGKGIRVESSFSSDMLQDLAWLQKRRGIKLYQRPEGTHTIKVRIYL LEAGLTRLDFMSDAGSRMKKFNQLLKRVMGKLYSLSIPDVLEEDEEESESEPEGQDIDELYQFVKRTHQQ EAKSVQVDVQHPALIPVLRPYQREAVNWMLQQEQFRSTPADNSLHFLWREIVTPDGLNLYYNPYTGCIIR DFPHAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVRQDALTLPEGKVVNYFIPTHCPRGKVKSREIQ NTECEPKEKVHCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSTFRYDVQRNRSLLKRMLKCLIFEGLVK QIKGRGFSGTFTLGKNYKEDVFDKTKKQAVGSPQKIEKELRKSVNKDTDSEYMPSDIYDDDNDNDDDDDD DPYYYYCKARKSRKLKKKPVLLTKKEKGQPVNLDSQVEAPAAGDCASADAHVSESTCVSENRQIQDAEDC VESPSPAAMELAQSNTASFCETPDYRFECICGEFDQIGRKPRVQCLKCHLWQHAKCVNYEEKNLKVKPFY CPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITY DVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGIN RWCVSGTPVQRGLEDLFGLVVFLGIEPYCVKHWWIRLLYHPYCKKNPQHLYSFIAKIMWRSAKKDVIDQI QIPPQTEEMHWLHFSPVERHFYHRQHEVCCQDAVVKLRKISDWALKLSSLDRRTVSSILYPLLRLRQACC HPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGRRAQYRAALALPPP ECVILSEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLVAKHPGIPPTLRDGRLEEEAKQL REHYMSKCNTEVAEAQQALQPVQQSIRELQRKVHSGSPWWLNVVHRAMEYAVDEELVQRVRNEISSNYKH QTDKLSMSEKFRDCRGLQFLLTTQMEELHKFQKLVREAVKKLEKPPSRDVIESATVCHLRPARLPLNCCV FCKADELFTEYESKLFSNTVKGQTAIFEEMIEDEEGLVDDRVPTTTRGLWAVSETERSMKAILSFARSHR FDVEYIDEGSVSMDLFEAWKKEYKLLHEYWMTLRNRVSAVDELAMATERLRVRHPKEPKPNPPVHHIIEP HEVEQNRIKLVNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCF CNECTSIIIEQYSVGSHRSSIKCAICRQTTSHKEVSYVFTSEKANQEDDIPVKGSHSTKVEAVVRTLMKI QLKDPGAKALVFSTWQDVLDIISKALTDNNMGFTQISRIKTFQENLSAFKYDPQINILLLPLHTGSNGLT IIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAERSHTSSSGKHSE ASALTVADLADLFTKESEELE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107470
ORF Size	5103 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107470.1, NP_001100940.1
RefSeq Size	7005 bp
RefSeq ORF	5106 bp
Locus ID	308282
MW	194.7 kDa

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