Shprh (NM_001107470) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202093 representing NM_001107470 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGCCGAAGGAAACGTGCTCCCCCGATGAAGGTGGATGAAGAAAGGCAGCAGCAGCTTCGCTGGA ACATGCACGAGGACCTGAGGAATGAGCCGCTCAGCTTGACTGAGGAAGAGCGGGCCTGCTGGGATGCAGA CTCCTCCGATTGCATCATCATAGATGATGACCCTCCCGAAGGTGCAGCTCACAGAGATAAGAAGAGGTGT ACAGAGTCTGTGAGCATCTTAAACTCCACTGAAAAAGAGACCCCTCTGTCCGTGGCCTTGAATGTTACCA TTTCTCCCCATCCTTCCGATGATTCTTGGAAAGCGTTTCTGGGAGATTTTGTGCTTCAGCTTCTCCCTGA AGAGAGTTTAGTTGAACATTTTTCTGAAAGGACTTTTACGTTGATGAGTTCAGAGTCAAGCAGTCAGCTC CTGATCTATGTTCATTCAGAATGTAACAATGTGGAGAAAGAAGAAAACGTACTTAAAGGACCAGCAAGTA TCTATGGCAAGGGTATTCGAGTGGAATCCTCCTTCAGTAGTGACATGTTACAGGATTTGGCCTGGCTACA GAAGAGAAGAGGAATAAAACTCTACCAGCGACCAGAAGGAACTCATACGATCAAGGTTAGAATTTACCTC TTGGAAGCTGGCCTGACAAGACTAGACTTCATGAGTGATGCAGGCTCCAGAATGAAAAAGTTCAATCAGC TCCTGAAGAGAGTGATGGGAAAGTTATACAGTCTCAGTATTCCAGATGTGTTGGAAGAAGATGAGGAGGA ATCAGAGAGTGAGCCAGAGGGCCAGGACATTGATGAGCTCTATCAGTTTGTGAAGCGAACACACCAGCAA GAGGCAAAGTCAGTCCAAGTGGATGTCCAGCACCCTGCACTGATTCCGGTGCTGAGGCCGTACCAGAGAG AGGCAGTCAACTGGATGCTACAGCAGGAGCAGTTCAGGAGCACTCCTGCTGATAACTCCCTGCACTTCTT GTGGCGAGAAATTGTTACACCTGATGGCTTGAACCTCTACTATAATCCGTACACAGGCTGCATCATTCGG GACTTCCCACATGCTGGGCCACAGTTGCTCGGTGGAATCTTAGCAGATGAGATGGGCCTCGGGAAGACAG TGGAGGTTCTGGCTCTGATTCTGACTCATACCCGACAAGATGTCAGACAGGATGCCCTCACCCTGCCTGA GGGAAAAGTGGTAAATTATTTCATCCCAACTCATTGTCCTAGAGGAAAAGTGAAAAGTAGAGAAATCCAG AATACAGAGTGTGAACCAAAAGAGAAAGTTCACTGCCCTCCCACACGTGTGATGATTCTGACAGCTGTGA AAGAAATGAATGGGAAAAAAGGCGTTTCTATTCTTTCTATCTATAAGTATGTCAGCTCCACATTCAGATA CGACGTTCAGCGGAACCGGAGTCTTCTGAAGCGGATGTTGAAATGTTTAATCTTTGAAGGTCTTGTGAAA CAGATTAAAGGCCGTGGTTTCTCTGGGACTTTTACACTGGGAAAGAATTACAAAGAAGATGTATTTGATA AAACGAAAAAGCAGGCAGTAGGGAGTCCACAAAAAATTGAGAAAGAGTTAAGGAAATCAGTGAACAAGGA TACAGATTCTGAATACATGCCATCAGACATTTATGATGACGATAATGACAACGACGATGACGACGACGAT GATCCTTATTATTATTACTGCAAGGCAAGAAAAAGCCGTAAGTTGAAGAAGAAGCCTGTGTTACTCACTA AAAAGGAAAAGGGTCAGCCTGTCAATCTGGATTCCCAAGTTGAGGCTCCAGCTGCTGGTGACTGTGCAAG CGCCGATGCTCATGTGTCAGAAAGTACATGTGTCTCTGAAAACAGGCAGATCCAAGACGCAGAGGACTGT GTTGAGTCTCCAAGCCCGGCCGCCATGGAGCTGGCACAGTCTAACACTGCAAGTTTCTGTGAGACCCCTG ACTACCGCTTTGAGTGTATATGTGGTGAATTTGACCAGATCGGCCGCAAGCCAAGAGTTCAGTGTCTGAA GTGTCACCTGTGGCAGCATGCAAAGTGTGTGAATTACGAGGAGAAGAATCTGAAGGTTAAGCCTTTTTAC TGCCCTCATTGCCTGGTTGCAATGGAGCCAGTGTCAACCAGAGCGACTCTCATCATCTCCCCAAGCTCCA TTTGCCACCAGTGGGTAGATGAAATCAACCGGCACGTGAGATCGTCATCCCTTCGAGTTTTGGTATATCA AGGAGTGAAGAAAGATGGGTTCTTACAGCCTCATTTTTTGGCAGAACAAGATATAGTTATCATTACCTAT GATGTTCTCCGTTCAGAATTAAACTATGTCGATATCCCACATAGCAATAGTGAAGATGGGCGTCGCCTGA GGAACCAGAAGCGCTACATGGCCATTCCCAGCCCCCTGGTAGCGGTGGAGTGGTGGCGGATCTGTCTGGA TGAAGCGCAGATGGTCGAGTGTCCTACAGTAAAAGCTGCAGAAATGGCCCAGCGTTTGAGTGGCATTAAT CGGTGGTGTGTCAGTGGCACTCCAGTGCAGAGAGGATTGGAAGATCTTTTTGGGCTGGTGGTCTTTCTTG GGATTGAACCTTATTGTGTCAAACACTGGTGGATTCGACTTCTCTATCATCCATACTGCAAGAAAAATCC TCAACACCTGTACAGTTTTATTGCTAAGATAATGTGGAGGTCCGCAAAGAAAGATGTCATTGACCAAATC CAGATCCCACCTCAGACTGAAGAAATGCACTGGCTCCATTTTTCCCCTGTGGAGAGGCATTTCTACCACC GCCAGCATGAGGTGTGCTGCCAGGATGCTGTGGTAAAGCTCAGGAAGATCTCGGACTGGGCCCTGAAGCT GAGCAGCTTGGACAGAAGGACGGTCTCCTCCATCCTGTACCCCCTGCTGAGACTCAGGCAGGCCTGCTGC CACCCACAGGCTGTGCGTGGGGAGTTCCTGCCACTCCAGAAGAGCACCATGACGATGGAGGAGTTGCTGA CATCTTTGCAGAAGAAGTGTGGAACAGAGTGTGAAGAGGCCCACCGGCAGCTAGTTTGTGCCCTTAACGG CTTAGCAGGCATCCACATCATTAAAGGTAGAAGAGCACAGTACAGAGCAGCCCTTGCTTTGCCTCCCCCA GAATGTGTCATTCTTAGTGAATACGCCTTGGCAGCGGAACTCTACAGAGAAGTTTTAAGGTCGTCTGAGG AGCACAAAGGAAAACTCAAAACTGATTCACTTCAAAGACTTCACGCCACACATAACCTGATGGAGCTCCT GGTAGCCAAACACCCAGGGATACCGCCCACCCTGAGAGATGGCAGACTTGAAGAAGAGGCCAAGCAGCTC CGAGAGCACTACATGAGCAAGTGTAACACAGAGGTGGCCGAAGCCCAGCAAGCTTTGCAGCCTGTGCAGC AGTCCATTCGGGAGCTCCAAAGGAAGGTTCATTCTGGTTCTCCTTGGTGGCTGAATGTAGTTCACAGAGC AATGGAGTATGCTGTTGACGAGGAGCTTGTTCAGCGCGTGCGAAATGAGATAAGCAGTAACTACAAGCAC CAAACGGACAAACTCTCCATGTCAGAGAAGTTCCGTGACTGTAGAGGTCTTCAGTTCTTACTTACAACAC AAATGGAAGAGCTGCACAAGTTCCAGAAGCTAGTGAGGGAGGCTGTGAAGAAGCTGGAGAAACCTCCGTC CCGGGATGTGATTGAGTCTGCAACAGTTTGCCACCTGCGGCCAGCTAGACTCCCTCTCAACTGCTGTGTC TTTTGTAAAGCTGATGAATTGTTCACAGAGTATGAGTCAAAGCTATTTTCTAACACTGTCAAAGGCCAGA CCGCCATCTTTGAAGAAATGATAGAAGATGAAGAAGGACTCGTGGATGATAGAGTCCCTACCACTACCAG AGGCCTGTGGGCCGTGAGCGAGACGGAGCGATCTATGAAAGCAATACTATCATTTGCAAGATCACATAGG TTTGACGTTGAATATATAGATGAAGGAAGTGTTTCAATGGATCTCTTCGAGGCATGGAAGAAGGAGTATA AGTTACTTCATGAATACTGGATGACTCTGAGAAACCGCGTATCCGCTGTTGATGAACTTGCAATGGCTAC AGAGCGACTAAGAGTGCGCCATCCCAAGGAGCCAAAGCCAAACCCACCCGTCCACCACATCATTGAACCA CACGAGGTTGAACAGAATCGTATAAAACTAGTAAATGATAAAGCTGTTGCTACTTCCCAGCTTCAGAAGA AACTTGGACAGCTTCTTTACCTGACAAATTTGGAGAAGTCTCAGGACAAAACATCAGGAGGTATTAATCC AGAGCCTTGTCCGATCTGTGCTCGGCAGTTGGGAAAACAGTGGGCAGTGCTGACCTGCGGACACTGCTTC TGTAATGAATGCACTTCAATTATAATTGAACAATACAGTGTGGGATCCCATCGAAGCTCCATTAAGTGTG CTATCTGCCGCCAGACCACATCACACAAAGAAGTCTCCTATGTCTTCACTTCAGAGAAGGCAAACCAAGA GGATGATATCCCTGTGAAGGGTAGCCATTCTACAAAAGTGGAAGCTGTGGTCAGAACTCTGATGAAAATA CAACTTAAAGATCCGGGGGCCAAAGCGCTCGTTTTCTCAACGTGGCAAGATGTGTTAGATATTATTTCAA AAGCTCTCACGGACAACAACATGGGATTCACACAAATCAGTCGCATTAAGACATTCCAGGAGAACCTTTC AGCATTTAAATATGATCCCCAGATCAATATTTTGCTGCTGCCCCTGCATACAGGCTCTAATGGATTAACT ATCATTGAAGCCACTCATGTCCTCCTGGTGGAACCGATACTGAATCCTGCCCATGAGCTTCAGGCCATTG GGAGAGTGCACCGAATTGGACAGACAAAACCTACTATTGTACATAGATTCTTAATTAAAGCAACAATAGA AGAGCGGATGCAGGCAATGCTAAAGACTGCTGAGAGGAGCCACACCAGCTCGTCTGGGAAGCATTCTGAA GCCTCTGCACTGACCGTGGCTGACCTGGCAGACCTGTTCACCAAAGAGAGTGAGGAGCTTGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107470
Insert Size	5106 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107470.1, NP_001100940.1
RefSeq Size	7005 bp
RefSeq ORF	5106 bp
Locus ID	308282

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