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Dnmt3b (NM_001003959) Rat Tagged ORF Clone
CAT＃: RR201942
- TrueORF®
Dnmt3b (Myc-DDK-tagged ORF) - Rat DNA methyltransferase 3B (Dnmt3b), (10 ug)
"NM_001003959" in other vectors (3)
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CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201942 representing NM_001003959
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGAGACAGCAGACATCTTAATGAAGAAGAGGGTGCTAGTGGGTATGAGGACTGTATCATTGTTA ATGGGAACTGTAGTGACCAGTCCTCGGACACGAAGGATGCTCCCTCACCCCCAGTCTTGGAGGCAATCTG CACAGAGCCAGTCTGTACACCAGAGACCAGAGGCCGCAGATCAAGCTCACGGCTGTCTAAGAGGGAGGTC TCCAGCCTTCTGAATTACACGCAGGACGTGGTAGGAGATGGAGATGGTGAAGCGGATGATGGAGATGGCT CTGATATTCTCATGATGCCAAAGCTCACCCGAGAGACCAAGGATGCCAGGACTCCCTCTGAAAGCCCAGC CGTCCGAACCCGAAACAGCAACAGTATCTCCAGCCTGGAGAGGCAAAGAACCTCCCCCAGAATCACGCGA GGCCGGCAGGGCCGCTACCACGTTCAGGAGTACCCCGTGGAGTTCCCAGCTACCAAGTCTCGGAGGCGGC GAGCATCGTCTTCAGCAAGCACGCCATGGTCATCCCCTGCCAGCATCGAGCTCATGGAAGATGTGACACC TAAGAGCAGCAGTACGCCATCGGTTGACTTGAGCCAGGACGGCCCTCAGGAGGGCATGGATGCCACACAG GTGGATGCGGAGAGCAGGGATGGTGACAGCACTGAGTATCAGGATGACAAGGAGTTTGGAATAGGTGACC TTGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCTGCCATGGTGGTGTCCTGGAAAGCCACCTCCAA GCGCCAGGCCATGCCCGGAATGCGCTGGGTACAGTGGTTTGGCGATGGCAAGTTTTCTGAGATCGCTGCT GACAAGCTGGTGGCTCTGGGTCTGTTCAGCCAGCACTTTAATCTGGCCACCTTCAATAAGCTGGTTTCTT ATAGGAAGGCCATGTACCACACTCTGGAGAAAGCCATGGTGCGAGCTGGCAAGACCTTCCCCAGCCGCCC TGGAGACTCACTGGAGGACCAGCTGAAGCCCATGCTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGG ATCGAGGGCCTCAAACCCAACAACAAGCAACCAGAGGTTCATAAGTCGAAGGTGCGTCGTTCAGGCAGTA GGAACTTAGAAGCCAGGAGACGCGAGAACAAAAGTCGAAGACGCACAACCATTGACTTTGCCGCTTCTGA GTACTCCACACCCCCTAAGCGCCTCAAGACAAATAGCTATGGTGGGAAGGACCGAGGGGAAGATGAGGAG AGCCGAGAACGGATGGCTTCTGATGTCACTAACAACAAGGGCAATCTGGAAGACCGCTGTCTGTCCTGCG GTAAGAAGAACCCTGTGTCCTTCCACCCCCTCTTTGAGGGTGGGCTCTGTCAGAGTTGCCGGGATCGCTT CCTGGAGCTCTTCTACATGTACGATGAGGACGGCTATCAGTCCTACTGCACCGTGTGCTGTGAGGGCCGT GAACTGCTTCTGTGCAGTAACACAAGCTGCTGCAGGTGCTTCTGTGTGGAGTGTCTGGAGGTGCTGGTGG GTACAGGCACAGCGGAGGATGCCAAGCTGCAGGAACCCTGGAGCTGCTATATGTGCCTCCCGCAGCGCTG CCATGGGGTCCTGCGGCGCAGGAAGGACTGGAACATGCGCCTGCAAGACTTCTTCACTACTGATCCTGAC CTGGAAGAGTTTGAGCCGCCCAAGTTGTACCCAGCGATTCCTGCAGCCAAAAGGAGGCCCATTAGGGTCC TGTCCCTGTTTGATGGAATTGCAACAGGGTACTTGGTGCTCAAGGACTTGGGTATTAAAGTGGAGAAGTA CGTTGCCTCTGAAGTCTGTGCAGAGTCCATTGCTGTAGGAACCATTAAGCATGAAGGCCAAATCAAATAT GTCAATGACGTCCGGAAAATCACCAAGAAAAATATTGAAGAGTGGGGCCCATTTGACTTGGTGATTGGCG GAAGCCCATGCAATGATCTTTCTAATGTCAATCCTGCCAGGAAAGGCCTGTATGAGGGCACAGGAAGGCT CTTCTTTGAGTTTTACCACTTGCTGAATTATACACGCCCCAAGGAGGGCGACAACCGTCCATTCTTCTGG ATGTTCGAGAATGTTGTGGCCATGAAAGTGAATGACAAGAAAGACATCTCCCGATTCCTGGCATGTAACC CAGTGATGATCGACGCCATCAAGGTTTCTGCTGCTCACAGGGCCCGGTACTTCTGGGGTAATCTACCTGG AATGAACAGGCCTGTGATAGCTTCAAAGAATGATAAGCTCGAGCTACAAGACTGCTTGGAGTTCAGTAGG ACAGCAAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCCATCAGACAGGGGAAAAACCAGC TTTTCCCTGTAGTCATGAATGGCAAGGATGACGTTCTGTGGTGCACTGAGCTCGAAAGGATCTTCGGGTT CCCAGCTCACTACACAGACGTGTCCAACATGGGCCGGGGCGCCCGCCAGAAGCTACTGGGAAGGTCCTGG AGTGTGCCAGTCATCAGACACCTGTTTGCCCCCTTGAAGGACTACTTTGCATGTGAAAGTGTGCCAGTCA TCAGACACCTGTTTGCCCCCTTGAAGGACTACTTTGCATGTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201942 representing NM_001003959
Red=Cloning site Green=Tags(s) MKGDSRHLNEEEGASGYEDCIIVNGNCSDQSSDTKDAPSPPVLEAICTEPVCTPETRGRRSSSRLSKREV SSLLNYTQDVVGDGDGEADDGDGSDILMMPKLTRETKDARTPSESPAVRTRNSNSISSLERQRTSPRITR GRQGRYHVQEYPVEFPATKSRRRRASSSASTPWSSPASIELMEDVTPKSSSTPSVDLSQDGPQEGMDATQ VDAESRDGDSTEYQDDKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMPGMRWVQWFGDGKFSEIAA DKLVALGLFSQHFNLATFNKLVSYRKAMYHTLEKAMVRAGKTFPSRPGDSLEDQLKPMLEWAHGGFKPTG IEGLKPNNKQPEVHKSKVRRSGSRNLEARRRENKSRRRTTIDFAASEYSTPPKRLKTNSYGGKDRGEDEE SRERMASDVTNNKGNLEDRCLSCGKKNPVSFHPLFEGGLCQSCRDRFLELFYMYDEDGYQSYCTVCCEGR ELLLCSNTSCCRCFCVECLEVLVGTGTAEDAKLQEPWSCYMCLPQRCHGVLRRRKDWNMRLQDFFTTDPD LEEFEPPKLYPAIPAAKRRPIRVLSLFDGIATGYLVLKDLGIKVEKYVASEVCAESIAVGTIKHEGQIKY VNDVRKITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYTRPKEGDNRPFFW MFENVVAMKVNDKKDISRFLACNPVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEFSR TAKLKKVQTITTKSNSIRQGKNQLFPVVMNGKDDVLWCTELERIFGFPAHYTDVSNMGRGARQKLLGRSW SVPVIRHLFAPLKDYFACESVPVIRHLFAPLKDYFACE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001003959 |
| ORF Size | 2577 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 4289 bp |
| RefSeq ORF | 2580 bp |
| Locus ID | 444985 |
| MW | 97 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201942 | Dnmt3b (untagged ORF) - Rat DNA methyltransferase 3B (Dnmt3b), (10 ug) |
CNY 10,930.00 |
|
| RR201942L3 | Lenti ORF clone of Dnmt3b (Myc-DDK-tagged ORF) - Rat DNA methyltransferase 3B (Dnmt3b), (10 ug) |
CNY 12,830.00 |
|
| RR201942L4 | Lenti ORF clone of Dnmt3b (mGFP-tagged ORF) - Rat DNA methyltransferase 3B (Dnmt3b), (10 ug) |
CNY 13,970.00 |
