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Tnk2 (NM_001008336) Rat Tagged ORF Clone
CAT＃: RR201546
- TrueORF®
Tnk2 (Myc-DDK-tagged ORF) - Rat tyrosine kinase, non-receptor, 2 (Tnk2), (10 ug)
"NM_001008336" in other vectors (3)
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CNY 16,720.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC94214 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201546 representing NM_001008336
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGGAGGAGGGAACAGGCTGGCTGCTGGAGCTGCTGTCTGAGGTGCAGCTACAACAGTATTTCC TGAGGCTCCGAGATGACCTCAACATTACCCGCCTGTCTCATTTTGAGTATGTCAAAAACGAAGACCTGGA AAAGATTGGCATGGGCCGGCCTGGCCAGAGGCGGCTATGGGAGGCTGTGAAGAGGAGGAAGGCCATGTGC AAACGCAAGTCATGGATGAGCAAGGTGTTCAGTGGAAAGCGGCAGGAGGCTGAGTTCCCTTCCCATCACT CTCAGAGCACCTTCCGGAAGCCCTCCCCCACCCCAGGGGGCCTGGCAGGGGAGGGGACCCTGCAGAGCCT CACCTGCCTCATTGGGGAGAAAGACCTGCGCCTGCTGGAGAAGCTGGGAGATGGCTCCTTTGGCGTGGTG CGCAGGGGTGAATGGGATGCCCCTGCAGGGAAGACGGTGAGTGTGGCCGTGAAGTGCCTGAAACCTGATG TGCTGAGCCAGCCCGAGGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCATTCGCTAGACCACCG AAACCTCATTCGCTTGTACGGTGTGGTGCTCACACCACCCATGAAGATGGTGACAGAGCTGGCCCCTCTG GGATCCTTGTTGGACCGGCTACGTAAACACCAAGGTCATTTCCTCTTGGGGACGCTGAGTCGCTATGCCG TGCAGGTGGCTGAGGGCATGGGCTACCTGGAGTCCAAGCGCTTCATTCACCGAGACCTGGCTGCTCGAAA TCTGCTTTTGGCCACCCGTGACCTGGTCAAGATTGGGGACTTTGGACTGATGCGAGCTCTGCCCCAGAAT GATGGCCACTATGTCATGCAAGAACATCGCAAGGTGCCCTTTGCCTGGTGTGCCCCTGAGAGCCTGAAGA CACGGACTTTCTCCCATGCCAGTGACACCTGGATGTTTGGGGTCACACTGTGGGAGATGTTTACGTATGG CCAGGAGCCCTGGATTGGCCTCAATGGCAGCCAGATCCTGCATAAGATTGACAAGGAAGGGGAGCGCCTG CCCCGGCCTGAGGACTGCCCCCAAGACATCTACAATGTCATGGTCCAGTGCTGGGCCCACAAGCCAGAGG ACAGACCCACATTTGTGGCCCTTCGGGACTTCCTGCTGGAGGCTCAGCCTACAGACATGCGGGCCCTTCA GGACTTCGAGGAGCCGGACAAGCTGCACATCCAGATGAACGACGTCATTACTGTCATTGAGGGAAGGGCT GAGAACTACTGGTGGCGTGGTCAGAACACACGGACGCTGTGTGTGGGACCCTTCCCTCGAAATGTGGTGA CCTCTGTGGCTGGCCTGTCAGCCCAGGACATCAGCCAGCCTCTACAGAATAGCTTCATTCACACAGGGCA TGGCGACAGTGACCCCCGCCACTGCTGGGGGTTCCCTGACAGGATCGATGAACTGTACCTGGGAAACCCC ATGGACCCCCCTGACCTGCTGAGTGTGGAACTGAGCACCTCGCGACCCACCCAGCATTTAGGACGGATGA AAAAGCCAACATATGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGACTTGGCCT GAGGAAGCCAGCCCTGCCCCGAGGGCTCTGGCTGGCAAAGCCCTCAGCCCGGGTGCCAGGCACCAAGGCA GGCCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGCGAGGAGCCTGTAGCCCCAACCCCACGGC CCTGTGCACCTTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCCTGCTGGACAAGACACCACCACAGAG CCCCACACGGGCACTGCCACGACCTTTACATCCTACACCTGTGGTGGACTGGGACGCTCGCCCGCTGCCC CCGCCCCCTGCCTACGATGATGTGGCCCAAGATGAGGATGACTTCGAGGTGTGCTCTATCAACAGCACCC TAGTGAGTGCAGGCCTCCCTACCGGGCCTAGCCAAGGCGAGACCAATTATGCGTTTGTACCTGAGCAGGC CCAGCTGCCCCCTGCCCTGGAGGATAACCTGTTCCTGCCACCGCAGGGTGGAGGCCAGCCACCCAGCTCA GCCCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCC AGCTGACCCCGTCCCCTACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCC TCGGCCCACACGTCCACGTGTGGGACTATCTCCAGCTCCCTTAGGTGAGGAAGAGGCAAGTCGGTGGCCT GGACCCTCCTCCCCTCCCCGAGTGCCTCCCCGGGAACCCCTGTCTCCTCAAGGGTCGAGGACCCCAAGCC CCCTAGTACCACCTGGCAGCTCCCCACTACCGCATCGGCTCTCGAGCTCACCTGGAAAGACCATGCCCAC CACCCAGAGCTTTGCCTCAGACCCTAAGTACGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCT GGCCCCTGCATCCTGCCCATTGTCCGTGATGGCAGGAAGGTCAGCAGCACCCACTATTACCTGCTGCCAG AGCGCCCTCCCTACCTCGAGCGCTACCAGCGCTTCCTGAGGGAGGCCCAGAGCCCCGAGGAGCCGGCCGC CCTGCCCGTTCCCCCACTGCTGCCCCCGCCTAGCACTCCAGCCCCTGCCGCCCCCACTGCCACCGTCCGA CCTATGCCTCAGGCTGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCGCAGC CACCATCCCTGAGGGCCAGTGCACGGCTGCCGCAGAGGGGCTGCCCAGGGGATGGGCAAGAGGCTGCCCG GCCAGCAGACAAAGTCCAGATGCTGCAGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCC CTGAGGAGCCACAGCTGGAGTATTCAGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTTGGGCTGG GTCTTCGGCCACGGGTGGAGTGCCACAAAGTGCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTG TCACCTTCTGGGGTCCTGTGGCCCTGCCCACCACAAACGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201546 representing NM_001008336
Red=Cloning site Green=Tags(s) MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNITRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMC KRKSWMSKVFSGKRQEAEFPSHHSQSTFRKPSPTPGGLAGEGTLQSLTCLIGEKDLRLLEKLGDGSFGVV RRGEWDAPAGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPL GSLLDRLRKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQN DGHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERL PRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRA ENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNP MDPPDLLSVELSTSRPTQHLGRMKKPTYDPVSEDPDPLSSDFKRLGLRKPALPRGLWLAKPSARVPGTKA GRSSGGEVTLIDFGEEPVAPTPRPCAPSLAQLAMDACSLLDKTPPQSPTRALPRPLHPTPVVDWDARPLP PPPAYDDVAQDEDDFEVCSINSTLVSAGLPTGPSQGETNYAFVPEQAQLPPALEDNLFLPPQGGGQPPSS AQTAEIFQALQQECMRQLQVPTGQLTPSPTPGGDDKPQVPPRVPIPPRPTRPRVGLSPAPLGEEEASRWP GPSSPPRVPPREPLSPQGSRTPSPLVPPGSSPLPHRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRA GPCILPIVRDGRKVSSTHYYLLPERPPYLERYQRFLREAQSPEEPAALPVPPLLPPPSTPAPAAPTATVR PMPQAAPDPKANFSTNNSNPGAQPPSLRASARLPQRGCPGDGQEAARPADKVQMLQAMVHGVTTEECQAA LRSHSWSIQRAAQYLKVEQLFGLGLRPRVECHKVLEMFDWNLEQAGCHLLGSCGPAHHKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001008336 |
| ORF Size | 3120 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001008336.1, NP_001008337.1 |
| RefSeq Size | 4250 bp |
| RefSeq ORF | 3123 bp |
| Locus ID | 303882 |
| UniProt ID | Q5U2X5 |
| MW | 115.1 kDa |
| Gene Summary | Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363', thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201546 | Tnk2 (untagged ORF) - Rat tyrosine kinase, non-receptor, 2 (Tnk2), (10 ug) |
CNY 16,720.00 |
|
| RR201546L3 | Lenti ORF clone of Tnk2 (Myc-DDK-tagged ORF) - Rat tyrosine kinase, non-receptor, 2 (Tnk2), (10 ug) |
CNY 18,340.00 |
|
| RR201546L4 | Lenti ORF clone of Tnk2 (mGFP-tagged ORF) - Rat tyrosine kinase, non-receptor, 2 (Tnk2), (10 ug) |
CNY 20,140.00 |
