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Nos2 (NM_012611) Rat Tagged ORF Clone
CAT＃: RR201250
- TrueORF®
Nos2 (Myc-DDK-tagged ORF) - Rat nitric oxide synthase 2, inducible (Nos2), (10 ug)
"NM_012611" in other vectors (5)
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CNY 7,984.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | iNos; Nos2a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201250 representing NM_012611
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGCCCCTGGAAGTTTCTCTTCAGAGTCAAATCCTACCAAGGTGACCTGAAAGAGGAAAAGGACA TTAACAACAACGTGGAGAAAACCCCAGGTGCTATTCCCAGCCCAACAACACAGGATGACCCTAAGAGTCA CAAGCATCAAAATGGTTTCCCCCAGTTCCTCACTGGGACTGCACAGAATGTTCCAGAATCCCTGGACAAG CTGCATGTGACTCCATCGACCCGCCCACAGCACGTGAGGATCAAAAACTGGGGCAATGGAGAGATTTTTC ACGACACCCTTCACCACAAGGCCACCTCGGATATCTCTTGCAAGTCCAAATTATGCATGGGGTCCATCAT GAACTCCAAGAGTTTGACCAGAGGACCCAGAGACAAGCCCACCCCAGTGGAGGAGCTTCTGCCTCAAGCC ATTGAATTCATTAACCAGTATTATGGCTCCTTCAAAGAGGCAAAAATAGAGGAACATCTGGCCAGGCTGG AAGCCGTAACAAAGGAAATAGAAACAACAGGAACCTACCAGCTCACTCTGGATGAGCTCATCTTTGCCAC CAAGATGGCCTGGAGGAACGCCCCTCGCTGCATCGGCAGGATTCAGTGGTCCAACCTGCAGGTCTTCGAT GCCCGGAGCTGTAGCACTGCATCAGAAATGTTCCAGCATATCTGCAGACACATACTTTACGCCACTAACA GTGGCAACATCAGGTCGGCCATTACTGTGTTCCCCCAGCGGAGCGATGGGAAGCATGACTTCCGGATCTG GAATTCCCAGCTCATCCGGTACGCTGGCTACCAGATGCCCGATGGCACCATCAGAGGGGATCCTGCCACC TTGGAGTTCACCCAGTTGTGCATCGACCTGGGCTGGAAGCCCCGCTATGGCCGCTTCGATGTGCTGCCTC TGGTCCTGCAGGCTCACGGTCAAGATCCAGAGGTCTTTGAAATCCCTCCTGATCTTGTGCTGGAGGTGAC CATGGAGCATCCCAAGTACGAGTGGTTCCAGGAGCTCGGGCTGAAGTGGTATGCGCTGCCTGCCGTGGCC AACATGCTCCTGGAGGTGGGTGGCCTCGAGTTCCCAGCCTGCCCCTTCAATGGTTGGTACATGGGCACCG AGATTGGAGTCCGAGACTTCTGTGACACACAGCGCTACAACATCCTGGAGGAAGTGGGCAGGAGCATGGG CCTGGAGACCCACACACTGGCCTCCCTCTGGAAAGACCGGGCTGTCACCGAGATCAATGCAGCTGTGCTC CATAGTTTTCAGAAGCAGAATGTGACCATCATGGACCACCACACAGCCTCAGAGTCCTTCATGAAGCACA TGCAGAATGAGTACCGGGCCCGAGGAGGCTGCCCTGCAGACTGGATTTGGCTGGTCCCTCCGGTGTCCGG GAGCATCACCCCTGTGTTCCACCAGGAGATGTTGAACTACGTCCTATCTCCATTCTACTACTACCAGATC GAGCCCTGGAAGACCCACATCTGGCAGGATGAGAAGCTGAGGCCCAGGAGGAGAGAGATCCGGTTCACAG TCTTGGTGAAAGCGGTGTTCTTTGCTTCTGTGCTAATGCGGAAGGTCATGGCTTCCCGCGTCAGAGCCAC AGTCCTCTTTGCTACTGAGACAGGAAAGTCGGAAGCGCTAGCCAGGGACCTGGCTGCCTTGTTCAGCTAC GCCTTCAACACCAAGGTTGTCTGCATGGAACAGTATAAGGCAAACACCTTGGAAGAGGAACAACTACTGC TGGTGGTTACAAGCACATTTGGCAATGGAGACTGCCCCAGCAATGGGCAGACTCTGAAGAAATCTCTGTT CATGATGAAAGAACTCGGGCATACCTTCAGGTATGCGGTATTTGGCCTGGGCTCCAGCATGTACCCTCAG TTCTGTGCCTTTGCTCATGACATCGACCAGAAACTGTCTCACCTGGGAGCCTCCCAGCTTGCCCCAACCG GAGAAGGGGACGAACTCAGCGGGCAGGAGGACGCCTTCCGCAGCTGGGCTGTGCAAACCTTCCGGGCAGC CTGTGAGACGTTCGATGTTCGAAGCAAACATTGCATTCAGATCCCGAAACGCTACACTTCCAACGCAACA TGGGAGCCAGAGCAGTACAAGCTCACCCAGAGCCCAGAGTCTCTAGACCTCAACAAAGCTCTCAGCAGCA TCCACGCCAAGAACGTGTTCACCATGAGGCTGAAATCCCTCCAGAATCTGCAGAGTGAGAAGTCCAGCCG CACCACCCTCCTTGTTCAACTCACCTTCGAGGGCAGCCGAGGCCCCAGCTACCTACCTGGGGAACACCTG GGGATTTTCCCAGGCAACCAGACGGCCCTGGTGCAAGGGATCTTGGAGCGAGTTGTGGATTGTTCTTCGC CAGACCAAACTGTGTGCCTGGAGGTTCTAGATGAGAGTGGCAGCTACTGGGTCAAAGACAAGAGGCTTCC CCCCTGCTCACTCAGGCAAGCCCTCACCTACTTCCTGGACATCACTACCCCTCCCACCCAGCTGCAGCTC CACAAGCTGGCCCGCTTTGCCACGGAAGAGACGCACAGGCAGAGGTTGGAGGCCTTGTGTCAGCCCTCAG AGTACAACGATTGGAAGTTCAGCAACAACCCCACGTTCCTGGAGGTGCTGGAAGAGTTCCCATCATTGCG TGTGCCTGCTGCCTTCCTGCTGTTGCAGCTCCCCATTCTGAAGCCCCGCTACTACTCCATCAGCTCCTCC CAGGACCACACCCCCTCGGAGGTCCACCTCACTGTGGCTGTGGTCACCTATCGCACCCGAGATGGTCAGG GTCCCCTGCACCATGGCGTCTGCAGCACTTGGATCAATAACCTGAAGCCCGAAGACCCAGTGCCCTGCTT TGTGCGGAGTGTCAGTGGCTTCCAGCTCCCTGAGGACCCCTCCCAGCCCTGCATCCTCATTGGGCCCGGT ACAGGCATTGCCCCCTTCCGAAGTTTCTGGCAGCAGCGGCTCCATGACTCTCAGCACAGAGGGCTCAAAG GAGGCCGCATGACCTTGGTGTTTGGGTGCAGGCACCCAGAGGAGGACCACCTCTATCAGGAAGAAATGCA GGAGATGGTCCGCAAGGGAGTGTTGTTCCAGGTGCACACAGGCTACTCCCGGCTGCCCGGAAAACCCAAG GTCTACGTTCAAGACATCCTGCAGAAAGAGCTGGCCGACGAGGTGTTCAGCGTGCTCCACGGGGAGCAGG GCCACCTTTATGTTTGTGGCGATGTGCGCATGGCTCGGGATGTGGCTACCACTTTGAAGAAGCTGGTGGC CGCCAAGCTGAACTTGAGTGAGGAGCAGGTTGAGGATTACTTCTTCCAGCTCAAGAGCCAGAAACGTTAT CATGAGGATATCTTCGGTGCGGTCTTTTCCTATGGAGCAAAAAAGGGCAACACCTTGGAGGAGCCCAAAG GCACAAGACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201250 representing NM_012611
Red=Cloning site Green=Tags(s) MACPWKFLFRVKSYQGDLKEEKDINNNVEKTPGAIPSPTTQDDPKSHKHQNGFPQFLTGTAQNVPESLDK LHVTPSTRPQHVRIKNWGNGEIFHDTLHHKATSDISCKSKLCMGSIMNSKSLTRGPRDKPTPVEELLPQA IEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFD ARSCSTASEMFQHICRHILYATNSGNIRSAITVFPQRSDGKHDFRIWNSQLIRYAGYQMPDGTIRGDPAT LEFTQLCIDLGWKPRYGRFDVLPLVLQAHGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVA NMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRSMGLETHTLASLWKDRAVTEINAAVL HSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQI EPWKTHIWQDEKLRPRRREIRFTVLVKAVFFASVLMRKVMASRVRATVLFATETGKSEALARDLAALFSY AFNTKVVCMEQYKANTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMMKELGHTFRYAVFGLGSSMYPQ FCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHCIQIPKRYTSNAT WEPEQYKLTQSPESLDLNKALSSIHAKNVFTMRLKSLQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHL GIFPGNQTALVQGILERVVDCSSPDQTVCLEVLDESGSYWVKDKRLPPCSLRQALTYFLDITTPPTQLQL HKLARFATEETHRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLRVPAAFLLLQLPILKPRYYSISSS QDHTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWINNLKPEDPVPCFVRSVSGFQLPEDPSQPCILIGPG TGIAPFRSFWQQRLHDSQHRGLKGGRMTLVFGCRHPEEDHLYQEEMQEMVRKGVLFQVHTGYSRLPGKPK VYVQDILQKELADEVFSVLHGEQGHLYVCGDVRMARDVATTLKKLVAAKLNLSEEQVEDYFFQLKSQKRY HEDIFGAVFSYGAKKGNTLEEPKGTRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012611 |
| ORF Size | 3441 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012611.3, NP_036743.3 |
| RefSeq Size | 3793 bp |
| RefSeq ORF | 3444 bp |
| Locus ID | 24599 |
| UniProt ID | Q06518 |
| MW | 130.6 kDa |
| Gene Summary | cytokine-inducible enzmyme involved in nitric oxide (NO) production [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201250 | Nos2 (untagged ORF) - Rat nitric oxide synthase 2, inducible (Nos2), (10 ug) |
CNY 18,430.00 |
|
| RR201250L1 | Lenti ORF clone of Nos2 (Myc-DDK-tagged ORF) - Rat nitric oxide synthase 2, inducible (Nos2), (10 ug) |
CNY 19,950.00 |
|
| RR201250L2 | Lenti ORF clone of Nos2 (mGFP-tagged ORF) - Rat nitric oxide synthase 2, inducible (Nos2), (10 ug) |
CNY 10,384.00 |
|
| RR201250L3 | Lenti ORF clone of Nos2 (Myc-DDK-tagged ORF) - Rat nitric oxide synthase 2, inducible (Nos2), (10 ug) |
CNY 19,950.00 |
|
| RR201250L4 | Lenti ORF clone of Nos2 (mGFP-tagged ORF) - Rat nitric oxide synthase 2, inducible (Nos2), (10 ug) |
CNY 21,950.00 |
