Dio2 (NM_031720) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Untagged ORF Clone
Synonyms	5DII; DIOII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201204 representing NM_031720 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTCCTCAGCGTAGACTTGCTGATCACTCTTCAGATTCTGCCAGTCTTTTTCTCCAACTGCCTCT TCCTGGCGCTCTATGACTCGGTCATTCTGCTCAAGCACGTGGCGCTGCTTCTCAGCCGCTCCAAGTCCAC TCGCGGAGAGTGGAGGCGCATGCTGACCTCAGAAGGACTACGCTGTGTCTGGAACAGCTTTCTCCTAGAC GCCTACAAACAGGTTAAATTGGGTGAAGATGCTCCCAATTCCAGTGTGGTGCACGTGTCCAATCCTGAAG CAGGTAACAATTGTGCCTCAGAGAAGACGGCGGATGGGGCTGAATGCCACCTTCTTGACTTTGCCAGTGC AGAGCGCCCACTGGTGGTCAACTTTGGTTCAGCGACCTGACCACCTTTTACTAGGCAACTGCCAGCCTTC CGCCAGTTGGTGGAAGAGTTCTCCTCGGTGGCTGACTTCCTGTTGGTATACATTGATGAGGCTCACCCTT CAGATGGCTGGGCAGTGCCTGGGGACTCCTCTATGTCTTTTGAGGTTAAGAAGCACCGGAACCAAGAGGA CCGATGTGCTGCAGCCCACCAGCTCCTGGAGCGTTTCTCCTTGCCGCCCCAGTGTCAAGTTGTGGCTGAC CGCATGGACAATAATGCCAACGTAGCTTATGGGGTAGCCTTTGAACGTGTGTGCATCGTGCAGAGACGGA AAATTGCTTACTTAGGAGGGAAGGGCCCCTTCAGCTATAACCTGCAAGAAGTCCGAAGTTGGCTGGAGAA GAATTTCAGCAAGAGATGAATTCTAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201204 representing NM_031720 Red=Cloning site Green=Tags(s) MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGEWRRMLTSEGLRCVWNSFLLD AYKQVKLGEDAPNSSVVHVSNPEAGNNCASEKTADGAECHLLDFASAERPLVVNFGSAT*PPFTRQLPAF RQLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSMSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVAD RMDNNANVAYGVAFERVCIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNFSKR*ILD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031720
ORF Size	798 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation	This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031720.4, NP_113908.3
RefSeq Size	1829 bp
RefSeq ORF	789 bp
Locus ID	65162
UniProt ID	P70551
MW	29.8 kDa
Gene Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is expressed in a few tissues in rat, including brain, pituitary and brown adipose tissue. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. Studies in rat suggest that this gene may play an important role in the regulation of lipid metabolism and thermogenesis, and in osteoarthritis pathogenesis by enhancing the chondrocyte hypertrophy and inflammatory response. This protein is a selenoprotein containing the non-standard amino acid, selenocysteine (Sec), which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Unlike the other two members (DIO1 and DIO3) of this enzyme family, the mRNA for this gene contains an additional in-frame UGA codon that has been reported (in human) to function either as a Sec or a stop codon, resulting in two potential isoforms with one or two Sec residues; however, only the upstream Sec (conserved with the single Sec residue found at the active site in DIO1 and DIO3) was shown to be essential for enzyme activity (PMID:10403186). In addition, the lack of conservation of the protein extension past the second TGA codon suggests that the one-Sec containing isoform represents the canonical form. [provided by RefSeq, Oct 2018]

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