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Dkc1 (NM_133419) Rat Tagged ORF Clone
CAT＃: RR200986
- TrueORF®
Dkc1 (Myc-DDK-tagged ORF) - Rat dyskeratosis congenita 1, dyskerin (Dkc1), (10 ug)
"NM_133419" in other vectors (3)
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CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Nap57 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200986 representing NM_133419
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGCGGAAGCCGCCATGACGTTCCCGAAGAAGCACAAGAAGAAGAAAGAGCGGAAGCCGCTGC CGGAAGCTGACGTGGCTGAGATCCAGCACGCGGAAGATTTCCTGATCAAACCGGAGTCCAAGGCGGCTCA GTTGGACACCTCCCAGTGGCCCCTGCTGCTCAAGAACTTTGACAGACTGAACGTGCGGACGACCCACTAC ACGCCCATCCCGTGCGGCTCCAACCCGCTGAAGAGGGAGATCGGGGAGTACGTCAGGACCGGCTTCATCA ACCTGGACAAGCCATCCAACCCCTCTTCGCACGAGGTCGTGGCCTGGATCCGCCGCATCCTGCGCGTGGA GAAGACGGGGCACAGCGGGACACTTGACCCCAAGGTCACCGGGTGCCTCATCGTCTGCATCGAGCGCGCC ACGCGCCTGGTCAAGTCACAGCAGAGCGCGGGCAAAGAGTATGTCGGGGTCGTGCGGCTGCACAATGCTA TCGAGGGGACCGCCCAGCTCTCCAGGGCCCTGGAGACCCTGACAGGCGCCCTGTTCCAGCGTCCCCCGCT CATCGCAGCAGTGAAGCGGCAGCTGCGCGTGCGCACAATCTATGAGAGCCGTGTTGTCGAGTATGACCCT GAGCGCAGGCTGGGTGTGTTCTGGGTGAGCTGCGAGGCTGGCACCTACATCCGGACGCTGTGCGTGCACC TGGGGCTGCTGCTGGGCGTCGGGGGGCAGATGCAGGAGCTGCGGCGCGTGCGCTCGGGGGTCGTGGGCGA GCGGGACCACATGGTGACCATGCACGACGTCCTGGACGCGCAGTATCTATACGACCACCACCGGGACGAG AGCTACCTGCGCCGCGTGGTTTTCCCACTGGAGAAGCTGCTGACGTCGCACAAGCGCCTGGTGATGAAGG ACAGTGCGGTCAACGCCATCTGCTACGGCGCGAAGATCATGCTCCCCGGCCTCCTGCGCTACGAGGACGG CATCGAGGTCAACCAGGAGGTCGTGGTCATCACCACCAAGGGCGAGGCCGTCTGCGTGGCCATCGCACTG ATGACGACGGCTGTGATCTCCACGTGCGACCACGGGGTAGTAGCGAAGATCAAGCGCGTCATCATGGAGC GGGACACCTACCCCCGCAAGTGGGGCCTGGGGCCGAAGGCAAGCCAGAAGAAGCAGCTGATCAAGCAGGG CCTCCTGGACAAACATGGCCGACCCACGGACGGCACCCCCGCCTCCTGGACGCGCGACTACGTGGACTAC AGTGACTCCAGCAAGAAAGCGACGGCCGCCGAAGCCACGCCGGGCCCTGGGGTCACCGCGGACGCCGCCA GCATCGTGAAAAGGAAACGCGATAGCGACAGCGACGCCGACGAGGCGACGCCCACTACCACGCCCCGGGT GAAGAAGGAGAAGAAGAAGAAAAAGGAGAAGGCGGACGGCGGGGAGGAGGCTGCGGAGGACGGGGACGGC GACGCCACAAGGAAGAAAAAGAAGAAGAAGGCAAGGGCGGCAGAGGAGCTCAGTGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200986 representing NM_133419
Red=Cloning site Green=Tags(s) MADAEAAMTFPKKHKKKKERKPLPEADVAEIQHAEDFLIKPESKAAQLDTSQWPLLLKNFDRLNVRTTHY TPIPCGSNPLKREIGEYVRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERA TRLVKSQQSAGKEYVGVVRLHNAIEGTAQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESRVVEYDP ERRLGVFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVVGERDHMVTMHDVLDAQYLYDHHRDE SYLRRVVFPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGLLRYEDGIEVNQEVVVITTKGEAVCVAIAL MTTAVISTCDHGVVAKIKRVIMERDTYPRKWGLGPKASQKKQLIKQGLLDKHGRPTDGTPASWTRDYVDY SDSSKKATAAEATPGPGVTADAASIVKRKRDSDSDADEATPTTTPRVKKEKKKKKEKADGGEEAAEDGDG DATRKKKKKKARAAEELSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133419 |
| ORF Size | 1527 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133419.1, NP_596910.1 |
| RefSeq Size | 1803 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 170944 |
| UniProt ID | P40615 |
| MW | 56.6 kDa |
| Gene Summary | nucleolar protein expressed in liver; colocalized with Nopp140 protein; may be involved with rRNA processing [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200986 | Dkc1 (untagged ORF) - Rat dyskeratosis congenita 1, dyskerin (Dkc1), (10 ug) |
CNY 6,560.00 |
|
| RR200986L3 | Lenti ORF clone of Dkc1 (Myc-DDK-tagged ORF) - Rat dyskeratosis congenita 1, dyskerin (Dkc1), (10 ug) |
CNY 8,460.00 |
|
| RR200986L4 | Lenti ORF clone of Dkc1 (mGFP-tagged ORF) - Rat dyskeratosis congenita 1, dyskerin (Dkc1), (10 ug) |
CNY 9,220.00 |
